Microarrays de DNA en el cáncer oral

Uno de los principales objetivos en la investigación sobre el cáncer en la actualidad es el estudio de marcadores que puedan predecir el pronóstico o la respuesta al tratamiento de forma individual. El número de genes implicados en los distintos pasos de la carcinogénesis oral aumenta a medida que se investiga sobre el tema. Los microarrays de DNA permiten el análisis simultáneo de la expresión de cientos de genes de un tejido en un solo experimento. El formato paralelo del ensayo permite el estudio de diferencias en la expresión genética entre células normales y enfermas, puesto que la actividad de cada gen en el microarray puede ser comparada en dos poblaciones celulares distintas. El objetivo de este trabajo es hacer una breve revisión de los estudios realizados por diversos autores que han intentado identificar genes relacionados con el cáncer oral, así como clasificarlo en subgrupos según los patrones de expresión genética; lo que permitirá una precoz detección, mejor diagnóstico y pronóstico del cáncer oral.One of the principal aims of modern cancer research is to identify markers allowing individual prediction of prognosis or response to treatment. In this connection, the number of genes thought to be involved in the different stages of different types of oral cancer increases apace. DNA microarrays allow simultaneous evaluation of the expression of hundreds of genes in a single assay. The parallel format of microassay slides is designed to allow rapid comparison of gene expression between two samples, for example tumor cells and healthy cells. This article reviews studies that have aimed to identify genes related to oral cancer, and to classify these genes into groups that are commonly co-expressed. These studies suggest that DNA microarrays are set to become routine tools in the detection, diagnosis, characterization and treatment of oral cancers

Area selection for the conservation of butterflies in the Iberian Peninsula and Balearic Islands

Coverage provided by the network of protected areas in the Iberian Peninsula and Balearic Islands was tested by measuring the coincidence between the squares protected by the network and the butterfly species recorded for such UTM grid squares. Five species were found to be absent in the network. The protected areas with the highest numbers of butterfly species were Ordesa National Park and Monte Perdido and the Posets-Maladeta Natural Park. Priority areas were selected using WORLDMAP software and showed that the all species of butterflies in the Iberian Peninsula and Balearic Islands can be found within 16 squares of 10×10 km (nine of them not within the network of protected areas). More specific area selections were also carried out: eight squares supported the total number of threatened species, five hosted all the Iberian endemisms and 13 harboured the rare butterfly species. This study detected 16 squares that are not currently protected but are important for butterfly conservation in the Iberian Peninsula and Balearic Islands

Modelling the scope to conserve an endemic-rich mountain butterfly taxon in a changing climate

Taxa restricted to mountains may be vulnerable to global warming, unless local-scale topographic variation and conservation actions can protect them against expected changes to the climate. We tested how climate change will affect the 19 mountain-restricted Erebia species of the Iberian Peninsula, of which 7 are endemic. To examine the scope for local topographic variation to protect against warming, we applied species distribution models (HadGEM2 and MPI) at two spatial scales (10 × 10 and 1 × 1 km) for two representative concentration pathways (RCP4.5 and RCP8.5) in 2050 and 2070. We also superimposed current and future ranges on the protected area (PA) network to identify priority areas for adapting Erebia conservation to climate change. In 10 × 10 km HadGEM2 models, climatically suitable areas for all species decreased in 2050 and 2070 (average −95.7%). Modelled decreases at 1 × 1 km were marginally less drastic (−95.3%), and 14 out of 19 species were still expected to lose their entire climatically favourable range by 2070. The PA network is well located to conserve the species that are expected to retain some climatically suitable areas in 2070. However, we identify 25 separate 10 × 10 km squares where new PAs would help to adapt the network to expected range shifts or contractions by Erebia. Based on our results, adapting the conservation of range-restricted mountain taxa to projected climate change will require the implementation of complementary in situ and ex situ measures alongside urgent climate change mitigationBiology Department from Universidad Autonoma de Madrid, Grant/Award Number: SBPLY/17/180501/000492; European Regional Development Fund; MCIU/AEI/ FEDER, UE, Grant/Award Number: RTI2018-096739-B-C21; NexTdive project, Grant/Award Number: PID2021-124187NBI00; Spanish Ministry of Science and Innovatio

Journey in teaching, as a teaching initiative in Architecture

In this paper, thanks to the experience of travel in the context of the Master of Conservation, is to analyze the figure of the student, as a traveler and not as a tourist, so this active travel is involved and how they may impact on improving his training as an architect. This remote mere spectator role also favors making efforts to know, identify, reflect, drawing, photograph or analyze what has been visited from a personal point of view and no doubt much more critical and free.En el presente trabajo, gracias a la experiencia del viaje en el contexto del Máster de Conservación, se propone analizar la figura del estudiante, en tanto que viajero y no turista, de modo que este participa activamente del viaje y cómo puede repercutir en la mejora de su formación como arquitecto. Este papel alejado del mero espectador, favorece también la realización de actividades encaminadas a conocer, identificar, reflexionar, dibujar, fotografiar o analizar aquello que se ha visitado desde un punto de vista personal y sin lugar a duda mucho más crítico y libre

Synthesis and Characterization of a New Cobalt(II) Complex with 2-(2-Pyridyl)Imino-N-(2-Thiazolin-2-yl)Thiazolidine (PyTT)

The compound aquanitrate-кObis[2-(2-pyridy)-imin-кN-N-(2-thiazin-кN-2-y)thiazidine]cbat() nitrate has been isolated and characterized by single crystal X-ray diffraction, IR spectroscopy, UV-Vis-NIR diffuse reflectance and magnetic susceptibility measurements. The environment around the cobalt atom may be described as a distorted octahedral geometry with the ligand-metal-ligand bite angles varying between 84.07(8)° and 98.66(8)°.The metallic atom is coordinated to two thiazoline nitrogens [av. Co-N =2.067 Å], two imino nitrogens [av. Co-N =2.122 Å], one oxygen atom of the nitrate group monodentate [Co-O(1)= 2.249(2) Å] and the oxygen atom of the water molecule [Co-O(IW)= 2.105(2) Å]. Electronic UV-Vis-NIR spectral data and the calculated magnetic moment are indicative of octahedral Co(ll) complexes. In the same way as other PyTT complexes, the organic moiety preserves the imino-thiazolidine form detected in the structure of PyTT

Synthetic matrix enhances transplanted satellite cell engraftment in dystrophic and aged skeletal muscle with comorbid trauma

Muscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promotes MuSC survival, proliferation, and differentiation. Furthermore, we establish a biomaterial-mediated cell delivery strategy for treating muscle trauma, where intramuscular injections may not be applicable. Delivery of MuSCs in the engineered matrix significantly improved in vivo cell survival, proliferation, and engraftment in nonirradiated and immunocompetent muscles of aged and dystrophic mice compared to collagen gels and cell-only controls. This platform may be suitable for treating craniofacial and limb muscle trauma, as well as postoperative wounds of elderly and dystrophic patients.Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the NIH under award numbers R21AR072287 (to Y.C.J.) and R01AR062368 (to A.J.G.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. This work was also funded by the Parker H. Petit Institute for Bioengineering and Bioscience Seed Grant Program (to A.J.G. and Y.C.J.)

Synthetic matrix enhances transplanted satellite cell engraftment in dystrophic and aged skeletal muscle with comorbid trauma

Muscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engineered a synthetic hydrogel-based matrix with optimal mechanical, cell-adhesive, and protease-degradable properties that promotes MuSC survival, proliferation, and differentiation. Furthermore, we establish a biomaterial-mediated cell delivery strategy for treating muscle trauma, where intramuscular injections may not be applicable. Delivery of MuSCs in the engineered matrix significantly improved in vivo cell survival, proliferation, and engraftment in nonirradiated and immunocompetent muscles of aged and dystrophic mice compared to collagen gels and cell-only controls. This platform may be suitable for treating craniofacial and limb muscle trauma, as well as postoperative wounds of elderly and dystrophic patients.Research reported in this publication was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases of the NIH under award numbers R21AR072287 (to Y.C.J.) and R01AR062368 (to A.J.G.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. This work was also funded by the Parker H. Petit Institute for Bioengineering and Bioscience Seed Grant Program (to A.J.G. and Y.C.J.)

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

Myc genes are a family of proto-oncogenes whose proteins are implicated in the regulation of cell proliferation, differentiation and apoptosis, and in regulating the activity of genes involved in cell division. The aim of the present study was to establish a quantitative description of the expression of c-myc and evaluate its relationship with other clinical and prognostic factors, as well as to establish a multivariate survival prediction model. This is a retrospective study of 68 patients diagnosed with oral squamous cell carcinoma (OSCC). We constructed a tissue microarray for investigating the expression of c-myc by immunohistochemistry. Statistical analyses were carried out, and a multivariate model that predicts survival was established. The average expression of c-myc was 50.32 (SD, 26.05) with a range from 6.60 to 99.48; similar for initial and advanced tumor stages. Non-smoking patients had higher levels of c-myc, showing statistically significant differences (Kruskal-Wallis chi2=5.975; p=0.05). We found no statistically significant relationship between the quantitative expression of c-myc and any other clinical or pathological parameters. For each unit of increase of c-myc, the risk increased by 1.15 (p<0.001; HR, 1.150; 95% CI, 1062-1245). Further study of this protein, which may have a significant diagnostic, prognostic and therapeutic value is warranted. Its determination can be valuable when used together with other markers to assess the prognosis of OSCC patients

Effectiveness of the Natura 2000 network in protecting Iberian endemic fauna

The Iberian Peninsula is a major European region of biodiversity, as it harbours more than 30% of European endemic species. Despite a number of studies having evaluated the ability of nature reserves to protect certain taxa, there is still a lack of knowledge on how Iberian endemic fauna are represented in these reserves. We detected biodiversity hotspots of Iberian endemicity and evaluated the effectiveness of the Natura 2000 network (N2000) in representing 249 endemic species from eight animal taxonomic groups (amphibians, mammals, freshwater fishes, reptiles, water beetles, butterflies, lacewings and dung beetles). We found that only the 10% of these Iberian endemic species are considered species of community interest (i.e. species included in the Annexes of the Habitats Directive). We conducted gap analyses and null models of representativeness in N2000. Generally, N2000 is effective in its representation of Iberian endemic fauna, although we detected species and few hotspots of endemism that were still not represented. It is necessary to declare a few new protected areas, thus enhancing N2000's effectiveness in the conservation of the Iberian endemic fauna. Although the aim of N2000 is to protect species listed in the Birds and Habitats Directives, the conservation status of endemic species from one of the most important areas of Europe in terms of biodiversity, could be also a concern for the European Union. Our results are useful in the context of the recent European Commission mandate calling for a ‘fitness check’ of the Birds and Habitats Directives. This approach could be also applicable to other regions with high value of endemicity.DS‐F was supported by a post‐doctoral contract funded by Universidad de Castilla‐La Mancha and the European Social Fund (ESF). PA was supported by a ‘Ramón y Cajal’ contract (RYC‐2011‐07670, MINECO). This research was partially funded by project POII11‐0277‐5747 (Junta de Castilla‐La Mancha).Peer Reviewe