Phylogenomics and systematics in Pseudomonas

© 2015 Gomila, Peña, Mulet, Lalucat and García-Valdés. The genus Pseudomonas currently contains 144 species, making it the genus of Gram-negative bacteria that contains the largest number of species. Currently, multilocus sequence analysis (MLSA) is the preferred method for establishing the phylogeny between species and genera. Four partial gene sequences of housekeeping genes (16S rRNA, gyrB, rpoB, and rpoD) were obtained from 112 complete or draft genomes of strains related to the genus Pseudomonas that were available in databases. These genes were analyzed together with the corresponding sequences of 133 Pseudomonas type strains of validly published species to assess their correct phylogenetic assignations. We confirmed that 30% of the sequenced genomes of non-type strains were not correctly assigned at the species level in the accepted taxonomy of the genus and that 20% of the strains were not identified at the species level. Most of these strains had been isolated and classified several years ago, and their taxonomic status has not been updated by modern techniques. MLSA was also compared with indices based on the analysis of whole-genome sequences that have been proposed for species delineation, such as tetranucleotide usage patterns (TETRA), average nucleotide identity (ANIm, based on MUMmer and ANIb, based on BLAST) and genome-to-genome distance (GGDC). TETRA was useful for discriminating Pseudomonas from other genera, whereas ANIb and GGDC clearly separated strains of different species. ANIb showed the strongest correlation with MLSA. The correct species classification is a prerequisite for most diversity and evolutionary studies. This work highlights the necessity for complete genomic sequences of type strains to build a phylogenomic taxonomy and that all new genome sequences submitted to databases should be correctly assigned to species to avoid taxonomic inconsistencies.Financial support was obtained from the Spanish MINECO through projects CGL2011-24318 and Consolider CSD2009-00006, as well as funds for competitive research groups from the Government of the Balearic Islands (the last two funds with FEDER cofunding). MG and AP were supported by a postdoctoral contract from the University of the Balearic IslandsPeer Reviewe

Uncertainty management at the airport transit view

Air traffic networks, where airports are the nodes that interconnect the entire system, have a time-varying and stochastic nature. An incident in the airport environment may easily propagate through the network and generate system-level effects. This paper analyses the aircraft flow through the Airport Transit View framework, focusing on the airspace/airside integrated operations. In this analysis, we use a dynamic spatial boundary associated with the Extended Terminal Manoeuvring Area concept. Aircraft operations are characterised by different temporal milestones, which arise from the combination of a Business Process Model for the aircraft flow and the Airport Collaborative Decision-Making methodology. Relationships between factors influencing aircraft processes are evaluated to create a probabilistic graphical model, using a Bayesian network approach. This model manages uncertainty and increases predictability, hence improving the system's robustness. The methodology is validated through a case study at the Adolfo Suárez Madrid-Barajas Airport, through the collection of nearly 34,000 turnaround operations. We present several lessons learned regarding delay propagation, time saturation, uncertainty precursors and system recovery. The contribution of the paper is two-fold: it presents a novel methodological approach for tackling uncertainty when linking inbound and outbound flights and it also provides insight on the interdependencies among factors driving performance

Atividade leishmanicida do extrato de Echinaster (Othilia) echinophorus

In this study, a methanolic extract from Echinaster (Othilia) echinophorus was evaluated for activity against Leishmania amazonensis. The extract showed activity against the promastigote and amastigote forms with IC50 values of 62.9 and 37.5 μg.mL-1 respectively. This extract showed a moderate toxicity on macrophages from BALB/c mice. A dose of 100 mg/kg/day was effective when administered during 15 days by intraperitoneal route to BALB/c mice infected experimentally.Neste estudo descreve-se o efeito de um extrato metanólico de Echinaster echinophorus spp. no parasita Leishmania amazonensis. Em testes com as formas promastigotas e amastigotas, o IC50 do extrato foi 62,9 e 37,5 μg.mL-1, respectivamente. O extrato também tem toxicidade moderada em macrófagos de camundongos BALB/c. O tratamento de camundongos BALB/c infectados com L. amazonensis com doses diárias de 100 mg/kg/dia via intraperitoneal durante 15 dias mostrou-se relativamente efetivo no controle da infecção. Esta investigação confirma a importância de produtos naturais como fonte para a descoberta de fármacos com funções anti-Leishmania

Draft genome sequence of Pseudomonas stutzeri strain B1SMN1, a nitrogen-fixing and naphthalene-degrading strain isolated from wastewater

Peer Reviewe

Modelo de Tutoría 4.0 y elaboración de protocolo de investigación

Introduction. The objective of this work was to establish the incidence of tutoring 4.0 on the development of the research protocol.  Method. An exploratory, cross-sectional, and correlational study was carried out with a sample of 100 students from a public university enrolled in the professional practice and social service system through a technology, device, or electronic network.  Results. Show that the elaboration of the antecedents of the problem was the hidden layer that explains the semantic weight of the tutoring on the elaboration of the protocol, although the research design limited the findings to the research sample, suggesting the extension of the model to others. hidden layers that the literature identifies as project financing. Discussion. The proposed model yields similar results to the reported literature regarding knowledge management as a determinant of knowledge production and transfer. Conclusion. Contrast of the model in other samples is recommended.Introducción - El objetivo de este trabajo fue establecer la incidencia de la tutoría 4.0 en el desarrollo del protocolo de investigación. Método - Se realizó un estudio exploratorio, transversal y correlacional con una muestra de 100 estudiantes de una universidad pública matriculados en el sistema de práctica profesional y servicio social a través de una tecnología, dispositivo o red electrónica. Resultados - Demostrar que la elaboración de los antecedentes del problema fue la capa oculta que explica el peso semántico de la tutoría en la elaboración del protocolo, aunque el diseño de investigación limitó los hallazgos a la nuestra de investigación, sugiriendo la extensión del modelo a otras capas ocultas que la literatura identifica como financiamiento de proyectos. Discusión - El modelo propuesto arroja resultados similares a la literatura reportada sobre la gestión del conocimiento como determinante de la producción y transferencia de conocimiento. Conclusión - Se recomienda el contraste del modelo en otras muestras

Degradació aeròbica d’hidrocarburs aromàtics per Pseudomonas i Roseobacters

Abstract not availabl

Consideraciones sobre la situación del 16 y 17 de febrero de 1989 en Canarias

Ponencia presentada en: II Simposio Nacional de Predictores, celebrado en 1990 en Madrid los días 20-22 de noviembr

Anaplasma phagocytophilum MSP4 and HSP70 proteins are involved in interactions with host cells during pathogen infection.

Anaplasma phagocytophilum transmembrane and surface proteins play a role during infection and multiplication in host neutrophils and tick vector cells. Recently, A. phagocytophilum Major surface protein 4 (MSP4) and Heat shock protein 70 (HSP70) were shown to be localized on the bacterial membrane, with a possible role during pathogen infection in ticks. In this study, we hypothesized that A. phagocytophilum MSP4 and HSP70 have similar functions in tick-pathogen and host-pathogen interactions. To address this hypothesis, herein we characterized the role of these bacterial proteins in interaction and infection of vertebrate host cells. The results showed that A. phagocytophilum MSP4 and HSP70 are involved in host-pathogen interactions, with a role for HSP70 during pathogen infection. The analysis of the potential protective capacity of MSP4 and MSP4-HSP70 antigens in immunized sheep showed that MSP4- HSP70 was only partially protective against pathogen infection. This limited protection may be associated with several factors, including the recognition of non-protective epitopes by IgG in immunized lambs. Nevertheless, these antigens may be combined with other candidate protective antigens for the development of vaccines for the control of human and animal granulocytic anaplasmosis. Focusing on the characterization of host protective immune mechanisms and protein-protein interactions at the host-pathogen interface may lead to the discovery and design of new effective protective antigens