10 research outputs found
e5: euskararen komunitatearen garapenerako marko interpretatibo berria
Lan honetan «Euskararen Etorkizuneko Eszenarioak Elkarrekin Eraikitzen» (e5) egitasmoa aurkeztuko da. e5 bi helburu nagusiren inguruan ardaztu zen: batetik, hurrengo hamarkadetarako euskalgintzaren aro berria konfiguratzen laguntzea, eta bestetik,
euskararen biziberritze prozesua ulertzeko eta garatzeko marko interpretatibo berri bat
lantzea. Artikuluan KOHiKoGa Eredua (Kapitaletan Oinarritutako Hizkuntz Komunitatearen Garapena) izendatu dugun marko berriaren oinarri teoriko-metodologikoak aurkeztuko ditugu eta e5 egitasmoa nola egikaritu zen azaldu. Azkenik, marko berri
hau maila mikroan euskara biziberritzeko tresna egokia dela iradokiko dugu
A Drastic Shift in Lipid Adducts in Colon Cancer Detected by MALDI-IMS Exposes Alterations in Specific K+ Channels
Even though colorectal cancer (CRC) is one of the most preventable cancers, it is one of the deadliest, and recent data show that the incidence in people <50 years has unexpectedly increased. While new techniques for CRC molecular classification are emerging, no molecular feature is as yet firmly associated with prognosis. Imaging mass spectrometry (IMS) lipidomic analyses have demonstrated the specificity of the lipid fingerprint in differentiating pathological from healthy tissues. During IMS lipidomic analysis, the formation of ionic adducts is common. Of particular interest is the [Na+]/[K+] adduct ratio, which already functions as a biomarker for homeostatic alterations. Herein, we show a drastic shift of the [Na+]/[K+] adduct ratio in adenomatous colon mucosa compared to healthy mucosa, suggesting a robust increase in K+ levels. Interrogating public databases, a strong association was found between poor diagnosis and voltage-gated potassium channel subunit beta-2 (KCNAB2) overexpression. We found this overexpression in three CRC molecular subtypes defined by the CRC Subtyping Consortium, making KCNAB2 an interesting pharmacological target. Consistently, its pharmacological inhibition resulted in a dramatic halt in commercial CRC cell proliferation. Identification of potential pharmacologic targets using lipid adduct information emphasizes the great potential of IMS lipidomic techniques in the clinical field.This study was supported in part by the Institute of Health Carlos III (CP12/03338 and PI16/02200), Basque Government (IT1162-19), and the EC (European Regional Development Fund, ERDF, CP12/03338). A.M.B. and J.B-E. hold predoctoral fellowships of the Govern Balear (Direcció General d’Innovació i Recerca, FPI/2160/2018 and FPI/1787/2015, respectively), co-funded by the ESF (European Social Fund). K.P.-R. contract was supported by the Govern Balear (Servei d’Ocupació de les IIles Balears and Garantia Juvenil, JQ-SP 18/17), co-funded by the ESF. G.B.-C. and D.H.L.’s contracts were supported by the Institute of Health Carlos III, co-funded by ERDF (Miguel Servet II program, CPII17/00005 and PI16/02200, respectively). We are grateful to SGiker Lipidomic Service (UPV/EHU, MICINN, GV/EG, ESF) for the expert advice and technical and human support in MALDI-IMS analysis. The publication fee in this Open Access journal is fully supported by the Liberi Call of the IdISBa
Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age
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Open AccessArticle
Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age
by Diego Delgado
1, Ane Miren Bilbao
2, Maider Beitia
1, Ane Garate
1, Pello Sánchez
1, Imanol González-Burguera
3,4, Amaia Isasti
4,5, Maider López De Jesús
4,5,6, Jone Zuazo-Ibarra
7, Alejandro Montilla
7 [OrcID] , María Domercq
7 [OrcID] , Estibaliz Capetillo-Zarate
7,8, Gontzal García del Caño
3,4 [OrcID] , Joan Sallés
4,5,6, Carlos Matute
7 and Mikel Sánchez
1,2,*
1
Advanced Biological Therapy Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain
2
Arthroscopic Surgery Unit, Hospital Vithas Vitoria, 01008 Vitoria-Gasteiz, Spain
3
Department of Neurosciences, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01008 Vitoria-Gasteiz, Spain
4
Bioaraba, Neurofarmacología Celular y Molecular, 01008 Vitoria-Gasteiz, Spain
5
Department of Pharmacology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), 01008 Vitoria-Gasteiz, Spain
6
Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), 28029 Madrid, Spain
7
Achucarro Basque Center for Neuroscience, CIBERNED and Departamento de Neurociencias, Universidad del País Vasco (UPV/EHU), 48940 Leioa, Spain
8
IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Francesca Santilli
Int. J. Mol. Sci. 2021, 22(4), 1725; https://doi.org/10.3390/ijms22041725
Received: 24 November 2020 / Revised: 12 January 2021 / Accepted: 3 February 2021 / Published: 9 February 2021
(This article belongs to the Section Molecular Endocrinology and Metabolism)
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Abstract
Platelet-rich plasma (PRP) is a biologic therapy that promotes healing responses across multiple medical fields, including the central nervous system (CNS). The efficacy of this therapy depends on several factors such as the donor’s health status and age. This work aims to prove the effect of PRP on cellular models of the CNS, considering the differences between PRP from young and elderly donors. Two different PRP pools were prepared from donors 65–85 and 20–25 years old. The cellular and molecular composition of both PRPs were analyzed. Subsequently, the cellular response was evaluated in CNS in vitro models, studying proliferation, neurogenesis, synaptogenesis, and inflammation. While no differences in the cellular composition of PRPs were found, the molecular composition of the Young PRP showed lower levels of inflammatory molecules such as CCL-11, as well as the presence of other factors not found in Aged PRP (GDF-11). Although both PRPs had effects in terms of reducing neural progenitor cell apoptosis, stabilizing neuronal synapses, and decreasing inflammation in the microglia, the effect of the Young PRP was more pronounced. In conclusion, the molecular composition of the PRP, conditioned by the age of the donors, affects the magnitude of the biological response.This work was funded by the Provincial Council of Alava through the AlavaInnova Program, Basque Government through the GAITEK Program, Ministry of Economy, Industry and Competitiveness (CTQ2017-85686-R), Spanish Ministry of Education and Science (PID2019-109724RB-I00), Basque Government (IT1203-19, IT1230-19, and KK-2020/00034) and Centro de Investigación Biomédica en Red en Enfermedades Neurodegenerativas (CIBERNED)
Microarray and Mass Spectrometry-Based Methodology for Lipid Profiling of Tissues and Cell Cultures
The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers in the context of different diseases and as indicators of the cell's homeostasis. However, further advances are required to unveil the complex relationships between lipid classes and lipid species with proteins. Here, we present a new methodology that combines microarrays with mass spectrometry to obtain the lipid fingerprint of samples of a different nature in a standardized and fast way, with minimal sample consumption. As a proof of concept, we use the methodology to obtain the lipid fingerprint of 20 rat tissues and to create a lipid library for tissue classification. Then, we combine those results with immunohistochemistry and enzymatic assays to unveil the relationship between some lipid species and two enzymes. Finally, we demonstrate the performance of the methodology to explore changes in lipid composition of the nucleus accumbens from mice subjected to two lipid diets.Impact de la composition lipidique membranaire sur la transmission dopaminergique dépendante du récepteur D2 et la motivatio
Deciphering the Lipid Architecture of the Rat Sciatic Nerve Using Imaging Mass Spectrometry
Knowledge on the normal structure
and molecular composition of
the peripheral nerves is essential to understand their pathophysiology
and to select the regeneration strategies after injury. However, the
precise lipid composition of the normal peripheral nerve is still
poorly known. Here, we present the first study of distribution of
individual lipids in the mature sciatic nerve of rats by imaging mass
spectrometry. Both positive and negative ion modes were used to detect,
identify and in situ map 166 molecular species of mainly glycerophospholipids,
sphingomyelins, sulfatides, and diacyl and triacylglycerols. In parallel,
lipid extracts were analyzed by LC-MS/MS to verify and complement
the identification of lipids directly from the whole tissue. Three
anatomical regions were clearly identified by its differential lipid
composition: the nerve fibers, the connective tissue and the adipose
tissue that surrounds the nerve. Unexpectedly, very little variety
of phosphatidylcholine (PC) species was found, being by far PC 34:1
the most abundant species. Also, a rich composition on sulfatides
was detected in fibers, probably due to the important role they play
in the myelin cover around axons, as well as an abundance of storage
lipids in the adipose and connective tissues. The database of lipids
here presented for each region and for the whole sciatic nerve is
a first step toward understanding the variety of the peripheral nerves’
lipidome and its changes associated with different diseases and mechanical
injuries
Optimized Protocol To Analyze Changes in the Lipidome of Xenografts after Treatment with 2Hydroxyoleic Acid
Influence of the Cation Adducts in the Analysis of Matrix-Assisted Laser Desorption Ionization Imaging Mass Spectrometry Data from Injury Models of Rat Spinal Cord
Imaging mass spectrometry
(IMS) is quickly becoming a technique
of reference to visualize the lipid distribution in tissue sections.
Still, many questions remain open, and data analysis has to be optimized
to avoid interpretation pitfalls. Here we analyze how the variation
on the [Na<sup>+</sup>]/[K<sup>+</sup>] relative abundance affects
the detection of lipids between sections of spinal cord of (uninjured)
control rats and of models of spinal cord demyelination and traumatic
contusion injury. The [M + Na]<sup>+</sup>/[M + K]<sup>+</sup> adducts
ratio remained approximately constant along transversal and longitudinal
sections of spinal cord from control animals, but it strongly changed
depending on the type of lesion. A substantial increase in the abundance
of [M + Na]<sup>+</sup> adducts was observed in samples from spinal
cord with demyelination, while the intensity of the [M + K]<sup>+</sup> adducts was stronger in those sections from mechanically injured
spinal cords. Such changes masked the modifications in the lipid profile
due to the injury and only after summing the signal intensity of all
adducts and corresponding monoprotonated molecular ions of each detected
lipid in a single variable, it was possible to unveil the real changes
in the lipid profile due to the lesion. Such lipids included glycerophospholipids
(both diacyl and aryl-acyl), sphingolipids, and nonpolar lipids (diacyl
and triacylglycerols), which are the main lipid classes detected in
positive-ion mode. Furthermore, the results demonstrate the sensitivity
of the technique toward modification in tissue homeostasis and that
the [M + Na]<sup>+</sup>/[M + K]<sup>+</sup> ratio may be used to
detect alterations in such homeostasis
MALDI-IMS files of 53 experiments over sections of nevus, primary melanoma and melanoma metastasis in imzml format
<p>MALDI-IMS files of 53 experiments over sections of nevus, primary melanoma and melanoma metastasis in imzml format. The experiments were recorded in negative-ion mode with a MALDI LTQ-orbitrap XL (ThermoFisher) at 25 um/pixel of spatial resolution. We also uploaded three .ppt with a comparison between the eosin-hematoxilin images, the IHC (MelanA and HMB45) and the segmentation images</p>