55 research outputs found
FUNCTIONAL POLYMORPHISM OF THE PRO-INFLAMMATORY CYTOKINE GENES IN PULMONARY TUBERCULOSIS
In the present time, incidence of pulmonary tuberculosis (TB) becomes broader, due to spreading resistance of Mycobacterium tuberculosis (MBT) to anti-tuberculosis drugs and infection with highly virulent strains of M. tuberculosis. The MBT antigens can cause dysfunction of the receptors and modulate the cytokine secreting function of immunocompetent cells. Polymorphic genes of pro-inflammatory cytokines involved in the mechanisms of defense responses of innate immunity, determine the degree of resistance to individual mycobacterial infection, as well as severity and duration of the disease in cases of clinical manifestations. The aim of the study was to investigate the connections between allelic polymorphisms of IL2, IFNG and TNFA genes and changes in secretion of the corresponding pro-inflammatory cytokines IL-2, IFNΞ³, and TNFΞ± in vitro in patients with the newly diagnosed pulmonary tuberculosis (TB), depending on the clinical form of the disease.A total of 334 patients (220 men and 114 women) aged 23 to 50 years with newly diagnosed infiltrative and disseminated TB were enrolled into the study. The control group consisted of 183 healthy donors (130 men and 53 women) of corresponding age. The material of the research included DNA extracted from the whole blood and supernatants of culture suspensions of mononuclear leukocytes isolated from venous blood in healthy volunteers and patients with TB. The evaluation of cytokines secretion was performed by measuring their concentration in the blood mononuclear cell culture supernatants. using enzyme-linked immunosorbent assay (ELISA). To study polymorphic regions of cytokine genes, a polymerase chain reaction (PCR) was applied. Analysis of the obtained data was carried out by means of the program Statistica for Windows Version 6.0 (StatSoft Inc., USA).It was found that the imbalance of secretion of pro-inflammatory cytokines in TB patients was associated with the polymorphic variants of genes of these cytokines. It was found that the hypo-secretion of IL-2 is determined by the carriage of the G allele and genotype GG (T-330G) of the IL2 gene in both the control group and in patients with TB, regardless of the clinical form. In patients with DTB carriers of the homozygous genotype TT (T-330G) of the IL2gene, increased protein secretion was established. The maximum secretion of TNFΠ± was recorded in patients with the AA genotype (G-308A) of the TNFA gene in the control group and in ITB patients; the minimum concentration of TNFΞ± was associated with the carrier of the homozygous GG genotype (G-308A) of the TNFA gene in all the examined groups. In patients with ITB and DTB, an increase in IFNΞ³ secretion by mononuclear blood leukocytes is not associated with the carrier of polymorphism +874A/T of the IFNG gene.Reduced secretion of IL-2 and TNFΞ± in TB patients is associated with polymorphisms of their genes β (T-330G) of IL2 gene and (G-308A) of TNFA gene, respectively. The polymorphism (+874A/T) of the IFNG gene does not have a modulatory effect on the secretion of IFNΞ³ in patients with TB, regardless of clinical form of the disease
Bio-nanotechnology application in wastewater treatment
The nanoparticles have received high interest in the ο¬eld of medicine and water puriο¬cation, however, the nanomaterials produced by chemical and physical methods are considered hazardous, expensive, and leave behind harmful substances to the environment. This chapter aimed to focus on green-synthesized nanoparticles and their medical applications. Moreover, the chapter highlighted the applicability of the metallic nanoparticles (MNPs) in the inactivation of microbial cells due to their high surface and small particle size. Modifying nanomaterials produced by green-methods is safe, inexpensive, and easy. Therefore, the control and modiο¬cation of nanoparticles and their properties were also discussed
Evolution of Sex-Specific Traits through Changes in HOX-Dependent doublesex Expression
Analysis in Drosophila suggests that evolutionary changes in the spatial regulation of the transcription factor doublesex play a key role in the origin, diversification, and loss of sex-specific structures
Monte Carlo Simulations of Metal-Poor Star Clusters
Metal-poor globular clusters (GCs) can provide a probe of the earliest epoch
of star formation in the Universe, being the oldest stellar systems observable.
In addition, young and intermediate-age low-metallicity GCs are present in
external galaxies. Nevertheless, inferring their evolutionary status by using
integrated properties may suffer from large \emph{intrinsic} uncertainty caused
by the discrete nature of stars in stellar systems, especially in the case of
faint objects. In this paper, we evaluate the \emph{intrinsic} uncertainty (due
to statistical effects) affecting the integrated colours and mass--to--light
ratios as a function of the cluster integrated visual magnitude (),
which represents a quantity directly measured. Our approach is based on Monte
Carlo techniques for randomly generating stars distributed according to the
cluster's mass function. Integrated colours and mass--to--light ratios in
different photometric bands are checked to be in good agreement with the
observational values of low-metallicity Galactic clusters. We present
integrated colours and mass--to--light ratios as a function of age for
different assumptions on the cluster total magnitude. We find that the
emph{intrinsic} uncertainty cannot be neglected. In particular, in models with
the broad-band colours show an \emph{intrinsic} uncertainty so
high as to prevent precise age evaluation of the cluster. Finally, the present
predictions are compared with recent results available in the literature,
showing in some cases non-negligible differences.Comment: 18 pages, 12 figures, A&A accepte
Π‘ΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΠΠ‘ΠΠ’-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ Π΄Π°Π½Π½ΡΡ ΠΏΡΠΈ ΠΎΡΡΡΡΡ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΡ ΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΡΠΎΠ²ΠΎΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΈΡ
Background The use of neuroimaging methods is an integral part of the process of assisting patients with acute cerebrovascular events (ACVE), and computed tomography (CT) is the Β«gold standardΒ» for examining this category of patients. The capabilities of the analysis of CT images may be significantly expanded with modern methods of machine learning including the application of the principles of radiomics. However, since the use of these methods requires large arrays of DICOM (Digital Imaging and Communications in Medicine)-images, their implementation into clinical practice is limited by the lack of representative sample sets. Inaddition, at present, collections (datasets) of CT images of stroke patients, that are suitable for machine learning, are practically not available in the public domain.Aim of study Regarding the aforesaid, the aim of this work was to create a DICOM images dataset of native CT and CT-angiography of patients with different types of stroke. Material and meth ods The collection was based on the medical cases of patients hospitalized in the Regional Vascular Center of the N.V. Sklifosovsky Research Institute for Emergency Medicine. We used a previously developed specialized platform to enter clinical data on the stroke cases, to attach CT DICOMimages to each case, to contour 3D areas of interest, and to tag (label) them. A dictionary was developed for tagging, where elements describe the type of lesion, location, and vascular territory.Results A dataset of clinical cases and images was formed in the course of the work. It included anonymous information about 220 patients, 130 of them with ischemic stroke, 40 with hemorrhagic stroke, and 50 patients without cerebrovascular disorders. Clinical data included information about type of stroke, presence of concomitant diseases and complications, length of hospital stay, methods of treatment, and outcome. The results of 370 studies of native CT and 102 studies of CT-angiography were entered for all patients. The areas of interest corresponding to direct and indirect signs of stroke were contoured and tagged by radiologists on each series of images.Conclusion The resulting collection of images will enable the use of various methods of data analysis and machine learning in solving the most important practical problems including diagnosis of the stroke type, assessment of lesion volume, and prediction of the degree of neurological deficit.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π½Π΅ΠΉΡΠΎΠ²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π΅ΠΎΡΡΠ΅ΠΌΠ»Π΅ΠΌΠΎΠΉ ΡΠ°ΡΡΡΡ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΎΠΊΠ°Π·Π°Π½ΠΈΡ ΠΏΠΎΠΌΠΎΡΠΈ Π±ΠΎΠ»ΡΠ½ΡΠΌ Ρ ΠΎΡΡΡΡΠΌΠΈ Π½Π°ΡΡΡΠ΅Π½ΠΈΡΠΌΠΈ ΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠ³ΠΎ ΠΊΡΠΎΠ²ΠΎΠΎΠ±ΡΠ°ΡΠ΅Π½ΠΈΡ (ΠΠΠΠ), ΠΏΡΠΈ ΡΡΠΎΠΌ Π·ΠΎΠ»ΠΎΡΡΠΌ ΡΡΠ°Π½Π΄Π°ΡΡΠΎΠΌ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π΄Π°Π½Π½ΠΎΠΉ ΠΊΠ°ΡΠ΅Π³ΠΎΡΠΈΠΈ Π±ΠΎΠ»ΡΠ½ΡΡ
ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½Π°Ρ ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΡ (ΠΠ’). ΠΠ½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎ ΡΠ°ΡΡΠΈΡΠΈΡΡ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π° ΠΠ’-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΌΠ°ΡΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΏΡΠΈΠ½ΡΠΈΠΏΠΎΠ² ΡΠ°Π΄ΠΈΠΎΠΌΠΈΠΊΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ, ΡΠ°ΠΊ ΠΊΠ°ΠΊ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΡΡΠ΅Π±ΡΠ΅Ρ Π½Π°Π»ΠΈΡΠΈΡ Π±ΠΎΠ»ΡΡΠΈΡ
ΠΌΠ°ΡΡΠΈΠ²ΠΎΠ² DICOM (Digital Imaging and Communications in Medicine)-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ, ΠΈΡ
Π²Π½Π΅Π΄ΡΠ΅Π½ΠΈΠ΅ Π² ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΡΡ ΠΏΡΠ°ΠΊΡΠΈΠΊΡ ΠΎΠ³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΎ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠΎΠΉ Π½Π°Π±ΠΎΡΠ° ΡΠ΅ΠΏΡΠ΅Π·Π΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΡ
Π²ΡΠ±ΠΎΡΠΎΠΊ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π² Π½Π°ΡΡΠΎΡΡΠ΅Π΅ Π²ΡΠ΅ΠΌΡ Π² ΠΎΡΠΊΡΡΡΠΎΠΌ Π΄ΠΎΡΡΡΠΏΠ΅ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈ Π½Π΅ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»Π΅Π½Ρ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΠ΅ ΠΠ’-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΡ Π±ΠΎΠ»ΡΠ½ΡΡ
c ΠΠΠΠ, ΠΊΠΎΡΠΎΡΡΠ΅ Π±ΡΠ»ΠΈ Π±Ρ ΠΏΡΠΈΠ³ΠΎΠ΄Π½Ρ Π΄Π»Ρ ΠΌΠ°ΡΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ.Π¦Π΅Π»Ρ Π ΡΠ²ΡΠ·ΠΈ Ρ Π²ΡΡΠ΅ΡΠΊΠ°Π·Π°Π½Π½ΡΠΌ, ΡΠ΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ ΡΠ²Π»ΡΠ»ΠΎΡΡ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ DICOM-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΠΠ’ ΠΈ ΠΠ’-Π°Π½Π³ΠΈΠΎΠ³ΡΠ°ΡΠΈΠΈ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΡΠΈΠΏΠ°ΠΌΠΈ ΠΠΠΠ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΡΠ½ΠΎΠ²ΠΎΠΉ Π΄Π»Ρ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΡΡΠ°Π»ΠΈ ΠΈΡΡΠΎΡΠΈΠΈ Π±ΠΎΠ»Π΅Π·Π½ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² ΡΠ΅Π³ΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΉ ΡΠΎΡΡΠ΄ΠΈΡΡΡΠΉ ΡΠ΅Π½ΡΡ ΠΠΠ Π‘Π ΠΈΠΌ. Π.Π. Π‘ΠΊΠ»ΠΈΡΠΎΡΠΎΠ²ΡΠΊΠΎΠ³ΠΎ. ΠΠ»Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»Π°ΡΡ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π½Π°Ρ Π½Π°ΠΌΠΈ ΡΠ°Π½Π΅Π΅ ΡΠΏΠ΅ΡΠΈΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΠΏΠ»Π°ΡΡΠΎΡΠΌΠ°, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡΠ°Ρ Π²Π²ΠΎΠ΄ΠΈΡΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΎ ΡΠ»ΡΡΠ°ΡΡ
ΠΠΠΠ, ΠΏΡΠΈΠΊΡΠ΅ΠΏΠ»ΡΡΡ ΠΊ ΠΊΠ°ΠΆΠ΄ΠΎΠΌΡ ΡΠ»ΡΡΠ°Ρ DICOM-ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΎΠΊΠΎΠ½ΡΡΡΠΈΠ²Π°ΡΡ ΠΈ ΡΠ΅Π³ΠΈΡΠΎΠ²Π°ΡΡ (ΡΠ°Π·ΠΌΠ΅ΡΠ°ΡΡ) 3D-ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ°. ΠΠ»Ρ ΡΠ΅Π³ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΡΠ»ΠΎΠ²Π°ΡΡ, ΡΠ»Π΅ΠΌΠ΅Π½ΡΡ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ ΠΎΠΏΠΈΡΡΠ²Π°ΡΡ ΡΠΈΠΏ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ, Π»ΠΎΠΊΠ°Π»ΠΈΠ·Π°ΡΠΈΡ ΠΈ Π±Π°ΡΡΠ΅ΠΉΠ½ ΠΊΡΠΎΠ²ΠΎΡΠ½Π°Π±ΠΆΠ΅Π½ΠΈΡ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ Π Ρ
ΠΎΠ΄Π΅ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»Π° ΡΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π° ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΡ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ»ΡΡΠ°Π΅Π² ΠΈ ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ, Π²ΠΊΠ»ΡΡΠ°ΡΡΠ°Ρ Π°Π½ΠΎΠ½ΠΈΠΌΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΎ 220 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ°Ρ
, ΠΈΠ· Π½ΠΈΡ
130 - Ρ ΠΈΡΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈΠ½ΡΡΠ»ΡΡΠΎΠΌ, 40 - Ρ Π³Π΅ΠΌΠΎΡΡΠ°Π³ΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΈΠ½ΡΡΠ»ΡΡΠΎΠΌ, Π° ΡΠ°ΠΊΠΆΠ΅ 50 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ Π±Π΅Π· ΡΠ΅ΡΠ΅Π±ΡΠΎΠ²Π°ΡΠΊΡΠ»ΡΡΠ½ΠΎΠΉ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ. ΠΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄Π°Π½Π½ΡΠ΅ Π²ΠΊΠ»ΡΡΠ°Π»ΠΈ ΡΠ²Π΅Π΄Π΅Π½ΠΈΡ ΠΎ ΡΠΈΠΏΠ΅ ΠΠΠΠ, Π½Π°Π»ΠΈΡΠΈΠΈ ΡΠΎΠΏΡΡΡΡΠ²ΡΡΡΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ ΠΈ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ, Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ, ΡΠΏΠΎΡΠΎΠ±Π΅ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΈ ΠΈΡΡ
ΠΎΠ΄Π΅. ΠΡΠ΅Π³ΠΎ Π΄Π»Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Π±ΡΠ»ΠΈ Π²Π²Π΅Π΄Π΅Π½Ρ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ 370 ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π½Π°ΡΠΈΠ²Π½ΠΎΠΉ ΠΠ’ ΠΈ 102 ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΠ’-Π°Π½Π³ΠΈΠΎΠ³ΡΠ°ΡΠΈΠΈ. ΠΠ° ΠΊΠ°ΠΆΠ΄ΠΎΠΉ ΡΠ΅ΡΠΈΠΈ ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ Π²ΡΠ°ΡΠΎΠΌ-ΡΠΊΡΠΏΠ΅ΡΡΠΎΠΌ Π±ΡΠ»ΠΈ ΠΎΠΊΠΎΠ½ΡΡΡΠ΅Π½Ρ ΠΈ ΠΏΡΠΎΡΠ΅Π³ΠΈΡΠΎΠ²Π°Π½Ρ ΠΎΠ±Π»Π°ΡΡΠΈ ΠΈΠ½ΡΠ΅ΡΠ΅ΡΠ°, ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΠ΅ ΠΏΡΡΠΌΡΠΌ ΠΈ ΠΊΠΎΡΠ²Π΅Π½Π½ΡΠΌ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌ ΠΠΠΠ.ΠΡΠ²ΠΎΠ΄ Π‘ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Π½Π°Ρ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΡ ΠΈΠ·ΠΎΠ±ΡΠ°ΠΆΠ΅Π½ΠΈΠΉ ΠΏΠΎΠ·Π²ΠΎΠ»ΠΈΡ Π² ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΌ ΠΏΡΠΈΠΌΠ΅Π½ΠΈΡΡ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π°Π½Π°Π»ΠΈΠ·Π° Π΄Π°Π½Π½ΡΡ
ΠΈ ΠΌΠ°ΡΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ΅Π½ΠΈΡ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠΈ Π²Π°ΠΆΠ½Π΅ΠΉΡΠΈΡ
ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π΄Π°Ρ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΡΠΈΠΏΠ° ΠΠΠΠ, ΠΎΡΠ΅Π½ΠΊΠΈ ΠΎΠ±ΡΠ΅ΠΌΠ° ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ, ΠΏΡΠΎΠ³Π½ΠΎΠ·Π° ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π½Π΅Π²ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΡΠΈΡΠΈΡΠ°
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