Self-Adjointness of Generalized MIC-Kepler System

We have studied the self-adjointness of generalized MIC-Kepler Hamiltonian, obtained from the formally self-adjoint generalized MIC-Kepler Hamiltonian. We have shown that for \tilde l=0, the system admits a 1-parameter family of self-adjoint extensions and for \tilde l \neq 0 but \tilde l <{1/2}, it has also a 1-parameter family of self-adjoint extensions.Comment: 11 pages, Latex, no figur

Measurement of Dielectric Suppression of Bremsstrahlung

In 1953, Ter-Mikaelian predicted that the bremsstrahlung of low energy photons in a medium is suppressed because of interactions between the produced photon and the electrons in the medium. This suppression occurs because the emission takes place over on a long distance scale, allowing for destructive interference between different instantaneous photon emission amplitudes. We present here measurements of bremsstrahlung cross sections of 200 keV to 20 MeV photons produced by 8 and 25 GeV electrons in carbon and gold targets. Our data shows that dielectric suppression occurs at the predicted level, reducing the cross section up to 75 percent in our data.Comment: 11 pages, format is postscript file, gzip-ed, uuencode-e

Bremsstrahlung Suppression due to the LPM and Dielectric Effects in a Variety of Materials

The cross section for bremsstrahlung from highly relativistic particles is suppressed due to interference caused by multiple scattering in dense media, and due to photon interactions with the electrons in all materials. We present here a detailed study of bremsstrahlung production of 200 keV to 500 MeV photons from 8 and 25 GeV electrons traversing a variety of target materials. For most targets, we observe the expected suppressions to a good accuracy. We observe that finite thickness effects are important for thin targets.Comment: 52 pages, 13 figures (incorporated in the revtex LaTeX file

Coordination as a function of skill level in the gymnastics longswing

The purpose of this study was to investigate the nature of inter-joint coordination at different levels of skilled performance to: (1) distinguish learners who were successful versus unsuccessful in terms of their task performance; (2) investigate the pathways of change during the learning of a new coordination pattern and (3) examine how the learner’s coordination patterns relate to those of experts in the longswing gymnastics skill. Continuous relative phase of hip and shoulder joint motions was examined for longswings performed by two groups of novices, successful (n = 4) and unsuccessful (n = 4) over five practice sessions, and two expert gymnasts. Principal component analysis showed that during longswing positions where least continuous relative phase variability occurred for expert gymnasts, high variability distinguished the successful from the unsuccessful novice group. Continuous relative phase profiles of successful novices became more out-of-phase over practice and less similar to the closely in-phase coupling of the expert gymnasts. Collectively, the findings support the proposition that at the level in inter-joint coordination a technique emerges that facilitates successful performance but is not more like an expert’s movement coordination. This finding questions the appropriateness of inferring development towards a “gold champion” movement coordination

The regulatory subunit of PKA-I remains partially structured and undergoes β-aggregation upon thermal denaturation

Background: The regulatory subunit (R) of cAMP-dependent protein kinase (PKA) is a modular flexible protein that responds with large conformational changes to the binding of the effector cAMP. Considering its highly dynamic nature, the protein is rather stable. We studied the thermal denaturation of full-length RIα and a truncated RIα(92-381) that contains the tandem cyclic nucleotide binding (CNB) domains A and B. Methodology/Principal Findings: As revealed by circular dichroism (CD) and differential scanning calorimetry, both RIα proteins contain significant residual structure in the heat-denatured state. As evidenced by CD, the predominantly α-helical spectrum at 25°C with double negative peaks at 209 and 222 nm changes to a spectrum with a single negative peak at 212-216 nm, characteristic of β-structure. A similar α→β transition occurs at higher temperature in the presence of cAMP. Thioflavin T fluorescence and atomic force microscopy studies support the notion that the structural transition is associated with cross-β-intermolecular aggregation and formation of non-fibrillar oligomers. Conclusions/Significance: Thermal denaturation of RIα leads to partial loss of native packing with exposure of aggregation-prone motifs, such as the B' helices in the phosphate-binding cassettes of both CNB domains. The topology of the β-sandwiches in these domains favors inter-molecular β-aggregation, which is suppressed in the ligand-bound states of RIα under physiological conditions. Moreover, our results reveal that the CNB domains persist as structural cores through heat-denaturation. © 2011 Dao et al

Лапароскопическая резекция локализованных образований паренхимы подковообразной почки с использованием 3D-печати: клиническое наблюдение

The use of 3D-computer technologies (3D-modeling, 3D-printing) in the surgical treatment of patients with kidney abnormalities contributes to a better understanding of anatomy at the preoperative planning stage and improves intraoperative navigation. We present a clinical observation using 3D-printing during performing organ-preserving laparoscopic surgery in a patient with localized formations of the horseshoe kidney parenchyma.Использование трехмерных (3D) компьютерных технологий (3D-моделирование, 3D-печать) в хирургическом лечении пациентов с заболеваниями аномалийных почек способствует лучшему пониманию анатомии на этапе предоперационного планирования и улучшает интраоперационную навигацию. Мы приводим клиническое наблюдение с использованием 3D-печати при выполнении органосохраняющей операции из лапароскопического доступа у пациента с локализованными образованиями паренхимы аномалийной подковообразной почки

Single-cell Atlas of common variable immunodeficiency shows germinal center-associated epigenetic dysregulation in B-cell responses

Common variable immunodeficiency (CVID), the most prevalent symptomatic primary immunodeficiency, displays impaired terminal B-cell differentiation and defective antibody responses. Incomplete genetic penetrance and ample phenotypic expressivity in CVID suggest the participation of additional pathogenic mechanisms. Monozygotic (MZ) twins discordant for CVID are uniquely valuable for studying the contribution of epigenetics to the disease. Here, we generate a single-cell epigenomics and transcriptomics census of naïve-to-memory B cell differentiation in a CVID-discordant MZ twin pair. Our analysis identifies DNA methylation, chromatin accessibility and transcriptional defects in memory B-cells mirroring defective cell-cell communication upon activation. These findings are validated in a cohort of CVID patients and healthy donors. Our findings provide a comprehensive multi-omics map of alterations in naïve-to-memory B-cell transition in CVID and indicate links between the epigenome and immune cell cross-talk. Our resource, publicly available at the Human Cell Atlas, gives insight into future diagnosis and treatments of CVID patients

A constitutive representation for linear aging, environmental-dependent viscoelastic materials

This study is concerned with developing a constitutive law for aging and environmental-dependent materials. The development rests on the assumption that the mechanical properties of the aforementioned materials can be mathematically represented by a functional of the strain and environmental histories, and this functional depends on the present time and the time the material is created. This constitutive assumption leads to two equivalent integral forms of the constitutive law after asserting that the functional is linear in the strain history. The first form of the integral law contains a material response functional analogous to the creep and relaxation functions of classical linear viscoelasticity. The second integral law has the same basic mathematical form but the physical interpretation of the mechanical response functional is different. It is demonstrated that both forms of the aging law reduce to the same non-aging law which is the usual starting for an analysis of only environmental-dependent materials. In the Appendix an operator algebra is presented for convenience in manipulating the integral laws for aging materials. Diese Untersuchung beschäftigt sich mit der Darstellung eines Werkstoffgesetzes für Altern und für umgebungsabhängige Stoffe. Die Entwicklung beruht auf der Annahme, daß die mechanischen Eigenschaften der oben erwähnten Stoffe mathematisch durch ein Funktional der Verzerrungs- und der Umgebungsgeschichten dargestellt werden kann und daß dieses Funktional von der augenblicklichen Zeit und der Zeit der Erzeugung des Stoffes abhängt. Diese Werkstoffannahme führt, unter der Voraussetzung einer linearen Abhängigkeit des Funktionals von der Verzerrungsgeschichte, auf zwei äquivalente Integraldarstellungen des Werkstoffgesetzes. Die erste Darstellung des Integralgesetzes enthält ein Materialantwort-Funktional analog der Kriech- und Relaxationsfunktionen der klassischen linearen Viskoelastizität. Das zweite Gesetz hat die gleiche mathematische Gestalt, aber verschiedene physikalische Interpretation des mechanischen Antwort-Funktionals. Es wird gezeigt, daß für nicht-alternde Stoffe beide Darstellungen dasselbe Gesetz, das der übliche Ausgangspunkt einer Untersuchung umgebungsbeeinflußter Stoffe ist, ergeben. Im Anhang werden einige für das Rechnen mit den Integralgesetzen alternder Stoffe handliche Sätze der Operatorenrechnung angegeben.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41700/1/707_2005_Article_BF01179657.pd

Adiabatic following and slow optical pulse propagation in rubidium vapor

Short pulses of narrow-line low-intensity dye-laser light nearly resonant with the Zeeman-split 2P1/2 esonance line (7948 A) of rubidium were observed to propagate through dilute rubidium vapor as slowly as (1/14)c. These slow pulse velocities showed that most of the energy in the propagating wave was contained by the vapor as coherent atomic excitation. The observed pulse velocities vp are in good agreement with the equation vp=dw/dk for the group velocity obtained from linear-dispersion theory. Also, the experimental results are quantitatively explained by adiabatic following, in which the pseudomoments of the atoms remain aligned along the effective field of the laser light. The adiabatic-following model allows for a direct comparison of our results with the work on self-induced transparency. For high-intensity light, adiabatic following predicts a nonlinear pulse velocity and the possibility of observing self-steepening.Peer reviewedElectrical and Computer Engineerin

Comet-FISH for the evaluation of plant DNA damage after mutagenic treatments

The aim of this study was to perform a comparative investigation of the actions of three mutagens that are widely used in plant mutagenesis using the comet-FISH technique. The comet-FISH technique was used for the analysis of DNA damage and the kinetics of repair within specific DNA sequences. FISH with rDNA and telomeric/centromeric DNA probes was applied to comets that were obtained from an alkaline/neutral comet assay. Migration within specific DNA sequences was analysed after treatment with two chemical mutagens-maleic hydrazide (MH) and N-nitroso-N-methylurea (MNU), and γ-rays. Barley was used as a model plant in this study. The possible utility of specific DNA sequences in a comparative assessment of the distribution of DNA damage within a plant genome was evaluated. This study proved that the comet-FISH technique is suitable for a detailed quantification of DNA damage and repair within specific DNA sequences in plant mutagenesis. The analysis of FISH signals demonstrated that the involvement of specific DNA sequences in DNA damage was different and was dependent on the mutagen used. We showed that 5S rDNA and telomeric DNA sequences are more sensitive to mutagenic treatment, which was expressed by a stronger fragmentation and migration in comparison to the other probes used in the study. We found that 5S rDNA and telomeric DNA probes are more suitable for testing the genotoxicity of environmental factors. A comparison of the involvement of specific chromosome domains in direct DNA breakage/repair and in chromosome aberration formation after mutagen treatment indicates the compatibility of the results