88 research outputs found

    Microarray analysis of mRNA from cumulus cells following in vivo or in vitro maturation of mouse cumulus-oocyte complexes

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    The IVM of mammalian cumulus–oocyte complexes (COCs) yields reduced oocyte developmental competence compared with oocytes matured in vivo. Altered cumulus cell function during IVM is implicated as one cause for this difference. We have conducted a microarray analysis of cumulus cell mRNA following IVM or in vivo maturation (IVV). Mouse COCs were sourced from ovaries of 21-day-old CBAB6F1 mice 46 h after equine chorionic gonadotrophin (5 IU, i.p.) or from oviducts following treatment with 5 IU eCG (61 h) and 5 IU human chorionic gonadotrophin (13 h). IVM was performed in α-Minimal Essential Medium with 50 mIU FSH for 17 h. Three independent RNA samples were assessed using the Affymetrix Gene Chip Mouse Genome 430 2.0 array (Affymetrix, Santa Clara, CA, USA). In total, 1593 genes were differentially expressed, with 811 genes upregulated and 782 genes downregulated in IVM compared with IVV cumulus cells; selected genes were validated by real-time reverse transcription–polymerase chain reaction (RT-PCR). Surprisingly, haemoglobin α (Hba-a1) was highly expressed in IVV relative to IVM cumulus cells, which was verified by both RT-PCR and western blot analysis. Because haemoglobin regulates O2 and/or nitric oxide availability, we postulate that it may contribute to regulation of these gases during the ovulatory period in vivo. These data will provide a useful resource to determine differences in cumulus cell function that are possibly linked to oocyte competence.Karen L. Kind, Kelly M. Banwell, Kathryn M. Gebhardt, Anne Macpherson, Ashley Gauld, Darryl L. Russell and Jeremy G. Thompso

    Towards understanding the impacts of the pet food industry on world fish and seafood supplies

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    The status of wild capture fisheries has induced many fisheries and conservation scientists to express concerns about the concept of using forage fish after reduction to fishmeal and fish oil, as feed for farmed animals, particularly in aquaculture. However, a very large quantity of forage fish is being also used untransformed (fresh or frozen) globally for other purposes, such as the pet food industry. So far, no attempts have been made to estimate this quantum, and have been omitted in previous fishmeal and fish oil exploitation surveys. On the basis of recently released data on the Australian importation of fresh or frozen fish for the canned cat food industry, here we show that the estimated amount of raw fishery products directly utilized by the cat food industry equates to 2.48 million metric tonnes per year. This estimate, plus the previously reported global fishmeal consumption for the production of dry pet food suggest that 13.5% of the total 39.0 million tonnes of wild caught forage fish is used for purposes other than human food production. This study attempts to bring forth information on the direct use of fresh or frozen forage fish in the pet food sector that appears to have received little attention to this date and that needs to be considered in the global debate on the ethical nature of current practices on the use of forage fish, a limited biological resource.<br /

    Cognitive radio scenarios for satellite communications: The CoRaSat approach

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    This paper presents initial results of the recently kicked-off FP7 ICT STREP project "CoRaSat" (Cognitive Radio for Satellite Communications) [1]. Focus is put on the preliminary identification of the scenarios which are suitable for the applicability of Cognitive Radio technology over Satellite Communications (SatCom). The considered frequency bands include Ka-band, Ku-band, C-band and S-band, where regulatory and coordination constraints exist. An initial mapping of broadband and narrowband SatCom use cases on each identified scenario is also provided. Moreover, several challenges associated to the applicability of Cognitive Radio over SatCom in the identified scenarios are presented, which form the basis of the market/business, regulatory, standardization and technological framework of CoRaSat. Furthermore, ongoing and future work of the CoRaSat project is outlined. © 2013 Authors

    Cognitive radio scenarios for satellite communications: The CoRaSat approach

    No full text
    This paper presents initial results of the recently kicked-off FP7 ICT STREP project "CoRaSat" (Cognitive Radio for Satellite Communications) [1]. Focus is put on the preliminary identification of the scenarios which are suitable for the applicability of Cognitive Radio technology over Satellite Communications (SatCom). The considered frequency bands include Ka-band, Ku-band, C-band and S-band, where regulatory and coordination constraints exist. An initial mapping of broadband and narrowband SatCom use cases on each identified scenario is also provided. Moreover, several challenges associated to the applicability of Cognitive Radio over SatCom in the identified scenarios are presented, which form the basis of the market/business, regulatory, standardization and technological framework of CoRaSat. Furthermore, ongoing and future work of the CoRaSat project is outlined. © 2013 Authors
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