Characterization of Archaeal Community in Contaminated and Uncontaminated Surface Stream Sediments

Archaeal communities from mercury and uranium-contaminated freshwater stream sediments were characterized and compared to archaeal communities present in an uncontaminated stream located in the vicinity of Oak Ridge, TN, USA. The distribution of the Archaea was determined by pyrosequencing analysis of the V4 region of 16S rRNA amplified from 12 streambed surface sediments. Crenarchaeota comprised 76% of the 1,670 archaeal sequences and the remaining 24% were from Euryarchaeota. Phylogenetic analysis further classified the Crenarchaeota as a Freshwater Group, Miscellaneous Crenarchaeota group, Group I3, Rice Cluster VI and IV, Marine Group I and Marine Benthic Group B; and the Euryarchaeota into Methanomicrobiales, Methanosarcinales, Methanobacteriales, Rice Cluster III, Marine Benthic Group D, Deep Sea Hydrothermal Vent Euryarchaeota 1 and Eury 5. All groups were previously described. Both hydrogen- and acetate-dependent methanogens were found in all samples. Most of the groups (with 60% of the sequences) described in this study were not similar to any cultivated isolates, making it difficult to discern their function in the freshwater microbial community. A significant decrease in the number of sequences, as well as in the diversity of archaeal communities was found in the contaminated sites. The Marine Group I, including the ammonia oxidizer Nitrosopumilus maritimus, was the dominant group in both mercury and uranium/nitrate-contaminated sites. The uranium-contaminated site also contained a high concentration of nitrate, thus Marine Group I may play a role in nitrogen cycle

Simulation for optimum crop production in irrigation systems adopting diversified crops during the dry season

Paper presented at the Philippine Society of Agricultural Engineers 40th Annual Convention, Punta Villa, Iloilo City, Philippines, 25-28 April 199

Baseline estimates of soil organic carbon by proximal sensing: Comparing design-based, model-assisted and model-based inference

For baselining and to assess changes in soil organic carbon (C) we need efficient soil sampling designs and methods for measuring C stocks. Conventional analytical methods are time-consuming, expensive and impractical, particularly for measuring at depth. Here we demonstrate the use of proximal soil sensors for estimating the total soil organic C stocks and their accuracies in the 0-10 cm, 0-30 cm and 0-100 cm layers, and for mapping the stocks in each of the three depth layers across 2837 ha of grazing land. Sampling locations were selected by probability sampling, which allowed design-based, model-assisted and model-based estimation of the total organic C stock in the study area. We show that spectroscopic and gamma attenuation sensors can produce accurate measures of soil organic C and bulk density at the sampling locations, in this case every 5 cm to a depth of 1 m. Interpolated data from a mobile multisensor platform were used as covariates in Cubist to map soil organic C. The Cubist map was subsequently used as a covariate in the model-assisted and model-based estimation of the total organic C stock. The design-based, model-assisted and model-based estimates of the total organic C stocks in the study area were similar. However, the variances of the model-assisted and model-based estimates were smaller compared to those of the design-based method. The model-based method produced the smallest variances for all three depth layers. Maps helped to assess variability in the C stock of the study area. The contribution of the spectroscopic model prediction error to our uncertainty about the total soil organic C stocks was relatively small. We found that in soil under unimproved pastures, remnant vegetation and forests there is good rationale for measuring soil organic C beyond the commonly recommended depth of 0-30 cm