Variabilité protéique observée par électrophorèse bidimensionnelle sur neuf isolats d'Heterodera schachtii provenant de six pays européens et sur deux isolats d'H. trifolii f.sp. beta

La variabilité protéique de neuf isolats européens d'#Heterodera schachtii et de deux isolats d'#H. trifolii f. sp. #beta élevés en conditions contrôlées sur colza, #Brassica napus L. var. #napus$ cv. Samouraï a été étudiée par électrophorèse bidimensionnelle (E2D). Les gels électrophorétiques sont comparés à l'aide d'un système informatique d'analyse d'images. Les indices de similarité (F) et les distances génétiques (D = 1-F) sont calculés à partir des taches homologues. Le dendrogramme est obtenu avec la méthode UPGMA. Les résultats montrent une assez grande similitude protéique des deux espèces. Les principales différences interspécifiques sont mises en évidence, ainsi que deux zones communes caractéristiques. Concernant la variabilité infraspécifique, des taches caratéristiques de certains isolats ont été repérées. La possibilité d'utiliser l'E2D pour identifier des protéines liées à la virulence est envisagée, ce qui nécessitera l'étude d'un grand nombre d'isolats. (Résumé d'auteur

A transient network of telechelic polymers and microspheres : structure and rheology

We study the structure and dynamics of a transient network composed of droplets of microemulsion connected by telechelic polymers. The polymer induces a bridging attraction between droplets without changing their shape. A viscoelastic behaviour is induced in the initially liquid solution, characterised in the linear regime by a stretched exponential stress relaxation. We analyse this relaxation in the light of classical theories of transient networks. The role of the elastic reorganisations in the deformed network is emphasized. In the non linear regime, a fast relaxation dynamics is followed by a second one having the same rate as in the linear regime. This behaviour, under step strain experiments, should induce a non monotonic behaviour in the elastic component of the stress under constant shear rate. However, we obtain in this case a singularity in the flow curve very different from the one observed in other systems, that we interpret in terms of fracture behaviour.Comment: 9 pages, 4 figure

Casimir forces in modulated systems

For the first time we present analytical results for the contribution of electromagnetic fluctuations into thermodynamic properties of modulated systems, like cholesteric or smectic liquid crystalline films. In the case of small dielectric anisotropy we have derived explicit analytical expressions for the chemical potential of such systems. Two limiting cases were specifically considered: (i) the Van der Waals (VdW) limit, i.e., in the case when the retardation of the electromagnetic interactions can be neglected; and (ii) the Casimir limit, i.e. when the effects of retardation becomes considerable. It was shown that in the Casimir limit, the film chemical potential oscillates with the thickness of the film. This non-monotonic dependence of the chemical potential on the film thickness can lead to step-wise wetting phenomena, surface anchoring reorientation and other important effects. Applications of the results may concern the various systems in soft matter or condensed matter physics with multilayer or modulated structures.Comment: 13 page

Bebida contendo abacaxi (Ananas comosus) e beterraba (Beta vulgaris) para crianças: tratar termicamente ou não?

Edição dos Resumos do VI Congresso Latinoamericano e XII Congresso Brasileiro de Higienistas de Alimentos, II Encontro Nacional de Vigilância das Zoonoses, IV Encontro do Sistema Brasileiro de Inspeção de Produtos de Origem Animal, Gramado, abr. 2013

Entropic phase separation of linked beads

We study theoretically a model system of a transient network of microemulsion droplets connected by telechelic polymers and explain recent experimental findings. Despite the absence of any specific interactions between either the droplets or polymer chains, we predict that as the number of polymers per drop is increased, the system undergoes a first order phase separation into a dense, highly connected phase, in equilibrium with dilute droplets, decorated by polymer loops. The phase transition is purely entropic and is driven by the interplay between the translational entropy of the drops and the configurational entropy of the polymer connections between them. Because it is dominated by entropic effects, the phase separation mechanism of the system is extremely robust and does not depend on the particlular physical realization of the network. The discussed model applies as well to other polymer linked particle aggregates, such as nano-particles connected with short DNA linkers

Time scales in shear banding of wormlike micelles

Transient stress and birefringence measurements are performed on wormlike micellar solutions that "shear band", i.e. undergo flow-induced coexistence of states of different viscosities along a constant stress "plateau". Three well-defined relaxation times are found after a strain rate step between two banded flow states on the stress plateau. Using the Johnson-Segalman model, we relate these time scales to three qualitatively different stages in the evolution of the bands and the interface between them: band destabilization, reconstruction of the interface, and travel of the fully formed interface. The longest timescale is then used to estimate the magnitude of the (unknown) "gradient" terms that must be added to constitutive relations to explain the history independence of the steady flow and the plateau stress selection

Dynamic Fluctuation Phenomena in Double Membrane Films

Dynamics of double membrane films is investigated in the long-wavelength limit including the overdamped squeezing mode. We demonstrate that thermal fluctuations essentially modify the character of the mode due to its nonlinear coupling to the transversal shear hydrodynamic mode. The corresponding Green function acquires as a function of the frequency a cut along the imaginary semi-axis. Fluctuations lead to increasing the attenuation of the squeezing mode it becomes larger than the `bare' value.Comment: 7 pages, Revte

Coexistence and Phase Separation in Sheared Complex Fluids

We demonstrate how to construct dynamic phase diagrams for complex fluids that undergo transitions under flow, in which the conserved composition variable and the broken-symmetry order parameter (nematic, smectic, crystalline, etc.) are coupled to shear rate. Our construction relies on a selection criterion, the existence of a steady interface connecting two stable homogeneous states. We use the (generalized) Doi model of lyotropic nematic liquid crystals as a model system, but the method can be easily applied to other systems, provided non-local effects are included.Comment: 4 pages REVTEX, 5 figures using epsf macros. To appear in Physical Review E (Rapid Communications