A view of the implanted SiC damage by Rutherford backscattering spectroscopy, spectroscopic ellipsometry, and transmission electron microscopy

4H-SiC single crystalline substrates were implanted at room temperature with 150 keV Al+ ions using fluences of 4 1014, 1 1015, and 2 1015 cm−2 with current density of 2.5 A cm−2. The samples were subsequently annealed at 1100 °C in N2 for 1 h in order to analyze their structural recovery. The disorder induced in both sublattices by the Al+ ions was studied by backscattering spectrometry in channeling geometry with a 3.5 MeV He2+ beam. The results were compared with the optical properties of the samples measured by spectroscopic ellipsometry. In a previous work, we concluded that during the postimplantation annealing of a highly damaged SiC crystalline material the short distance order can be recovered, while the long distance disorder remains. We also presented the possibility to have grains of different polytypes oriented faraway from the original direction. Now, this alternative is confirmed by the cross-sectional transmission and high resolution electron microscopy studies, carried out to obtain information about the crystal structure.Ministerio de Cienncias y Tecnología español-MAT2002-02843 (70% de fondos FEDER)Acuerdo de Colaboración Científica Húngaro-Españo-lE27 / 2001Fondo de Investigaciones Científicas de Hungría (OTKA)-T032029, T047011, T043704 y K6172

Altered myogenesis and premature senescence underlie human TRIM32-related myopathy

TRIM32 is a E3 ubiquitin -ligase containing RING, B-box, coiled-coil and six C-terminal NHL domains. Mutations involving NHL and coiled-coil domains result in a pure myopathy (LGMD2H/STM) while the only described mutation in the B-box domain is associated with a multisystemic disorder without myopathy (Bardet-Biedl syndrome type11), suggesting that these domains are involved in distinct processes. Knock-out (T32KO) and knockin mice carrying the c.1465G > A (p.D489N) involving the NHL domain (T32KI) show alterations in muscle regrowth after atrophy and satellite cells senescence. Here, we present phenotypical description and functional characterization of mutations in the RING, coiled-coil and NHL domains of TRIM32 causing a muscle dystrophy. Reduced levels of TRIM32 protein was observed in all patient muscle studied, regardless of the type of mutation (missense, single amino acid deletion, and frameshift) or the mutated domain. The affected patients presented with variable phenotypes but predominantly proximal weakness. Two patients had symptoms of both muscular dystrophy and Bardet-Biedl syndrome. The muscle magnetic resonance imaging (MRI) pattern is highly variable among patients and families. Primary myoblast culture from these patients demonstrated common findings consistent with reduced proliferation and differentiation, diminished satellite cell pool, accelerated senescence of muscle, and signs of autophagy activation.Health Institute Carlos III PI16-01843 JR15/00042FEDER PI16-01843 JR15/00042Fundación Progreso y Salud, Junta de Andalucía PI-0085-2016Australian National Health and Medical Research Council (NHMRC) APP1122952 APP111751

Altered myogenesis and premature senescence underlie human TRIM32-related myopathy

TRIM32 is a E3 ubiquitin -ligase containing RING, B-box, coiled-coil and six C-terminal NHL domains. Mutations involving NHL and coiled-coil domains result in a pure myopathy (LGMD2H/STM) while the only described mutation in the B-box domain is associated with a multisystemic disorder without myopathy (Bardet-Biedl syndrome type11), suggesting that these domains are involved in distinct processes. Knock-out (T32KO) and knockin mice carrying the c.1465G > A (p.D489N) involving the NHL domain (T32KI) show alterations in muscle regrowth after atrophy and satellite cells senescence. Here, we present phenotypical description and functional characterization of mutations in the RING, coiled-coil and NHL domains of TRIM32 causing a muscle dystrophy. Reduced levels of TRIM32 protein was observed in all patient muscle studied, regardless of the type of mutation (missense, single amino acid deletion, and frameshift) or the mutated domain. The affected patients presented with variable phenotypes but predominantly proximal weakness. Two patients had symptoms of both muscular dystrophy and Bardet-Biedl syndrome. The muscle magnetic resonance imaging (MRI) pattern is highly variable among patients and families. Primary myoblast culture from these patients demonstrated common findings consistent with reduced proliferation and differentiation, diminished satellite cell pool, accelerated senescence of muscle, and signs of autophagy activation.Health Institute Carlos III PI16-01843 JR15/00042FEDER PI16-01843 JR15/00042Fundación Progreso y Salud, Junta de Andalucía PI-0085-2016Australian National Health and Medical Research Council (NHMRC) APP1122952 APP111751

Intelligence architecture to detect patterns in scanned documents

La Inteligencia Artificial (en adelante IA) puede ser útil para aumentar la eficiencia en el abordaje de los casos que los Poderes Judiciales deben resolver. Sin embargo, los sistemas judiciales en países de Latinoamérica cuentan con ciertos desafíos para la aplicación de sistemas de IA que deben ser superados si se desea aprovechar los beneficios de la automatización inteligente. Los documentos, a lo largo de su ciclo de vida, suelen estar sujetos a escaneos, impresiones y retrabajos en ambos sentidos. En esta presentación se propone una forma alternativa a la de los reconocedores ópticos de caracteres (OCR), basada en IA aplicada a la detección de patrones en imágenes, que puede contribuir a paliar las dificultades mencionadas; no sólo en el ámbito del caso de estudio aplicado, sino en muy diversos dominios de sistemas de gestión de documentos públicos en la región.Artificial Intelligence (AI hereinafter) can be useful to increase efficiency in dealing with cases that the Judiciary must resolve. However, judicial systems in Latin American countries have certain challenges for the application of AI systems that must be overcome if the benefits of intelligent automation are to be exploited. Documents, throughout their life cycle, are often subject to scanning, printing and reworking in both directions. In this paper we propose an alternative way to OCR, based on AI applied to image pattern detection, which can contribute to alleviate the difficulties mentioned above, not only in the applied case study, but also in many different domains of public document management systems in the regionSociedad Argentina de Informática e Investigación Operativ

Efectividad de la vacunación contra la fiebre porcina clásica en cerdos tratados con aflatoxina b 1.

Para determinar si la intoxicación con aflatoxinas en cerdos es una de las causas de falla vacunal o reversión patogénica de la cepa vacunal en la fiebre porcina clásica, se formaron cuatro grupos de cerdos a los que se les administró 0, 0.4, 0.6 6 1.0 p

Efecto del entrenamiento de fuerza en deportistas femeninas de deportes colectivos mediante tecnología isoinercial

The aim of this research is to analyse the effect of a four-week strength programme using isoinertial technology on performance improvement in women in team sports on an intermittent basis. The sample was composed of six women with an average age of 21 (±1.79 years). The tests of V-cut direction changes, explosive force counter movement jump and repeated sprint ability over 40 m were performed. The main results show that all the variables analysed are improved by training with isoinertial technology. They present significant differences (p<.05) in the variables of explosive strength and repeated sprint ability. Therefore, a strength training program using isoinertial technology during four weeks of training is sufficient for the improvement of direction changes, resistance to repeated sprinting and explosive leg strength.El objetivo de esta investigación es analizar el efecto de un programa de fuerza mediante tecnología isoinercial durante cuatro semanas, en la mejora del rendimiento en mujeres de deportes colectivos con carácter intermitente. La muestra estaba compuesta por seis mujeres con una edad media de 21±1.79 años. Se realizaron los test de cambios de dirección V-cut, fuerza explosiva counter movement jump y repeated sprint ability sobre 40 m. Los principales resultados muestran que se mejoraron todas las variables analizadas mediante el entrenamiento con tecnología isoinercial. Además, presentaron diferencias significativas (p<.05) en las variables de fuerza explosiva y de repeated sprint ability. Por lo tanto, un programa de entrenamiento de fuerza a través de tecnología isoinercial durante cuatro semanas de entrenamiento es suficiente para la mejora de los cambios de dirección, la resistencia a la repetición de esprines y la fuerza explosiva de piernas

Effect of the addition of alginate and/or tetracycline on brushite cement properties

Calcium phosphate cements have the advantage that they can be prepared as a paste that sets in a few minutes and can be easily adapted to the shape of the bone defect, which facilitates its clinical application. In this research, six formulations of brushite (dicalcium phosphate dihydrated) cement were obtained and the effect of the addition of sodium alginate was analyzed, such as its capacity as a tetracycline release system. The samples that contain sodium alginate set in 4 or 5 min and showed a high percentage of injectability (93%). The cements exhibit compression resistance values between 1.6 and 2.6 MPa. The drug was released in a range between 12.6 and 13.2% after 7 days. The antimicrobial activity of all the cements containing antibiotics was proven. All samples reached values of cell viability above 70 percent. We also observed that the addition of the sodium alginate and tetracycline improved the cell viability.Imaging- and therapeutic targets in neoplastic and musculoskeletal inflammatory diseas

Centralized Inverted Decoupling Control

This paper presents a new methodology of multivariable centralized control based on the structure of inverted decoupling. The method is presented for general n×n processes, obtaining very simple general expressions for the controller elements with a complexity independent of the system size. The possible configurations and realizability conditions are stated. Then, the specification of performance requirements is carried out from simple open loop transfer functions for three common cases. As a particular case, it is shown that the resulting controller elements have PI structure or filtered derivative action plus a time delay when the process elements are given by first order plus time delay systems. Comparisons with other works demonstrate the effectiveness of this methodology through the use of several simulation examples and an experimental lab process

Heterozygous CAPN3 missense variants causing autosomal-dominant calpainopathy in seven unrelated families

[Aims] Recessive variants in CAPN3 gene are the cause of the commonest form of autosomal recessive limb girdle muscle dystrophy. However, two distinct in-frame deletions in CAPN3 (NM_000070.3:c.643_663del21 and c.598_621del15) and more recently, Gly445Arg and Arg572Pro substitutions have been linked to autosomal dominant (AD) forms of calpainopathy. We report 21 affected individuals from seven unrelated families presenting with an autosomal dominant form of muscular dystrophy associated with five different heterozygous missense variants in CAPN.[Methods] We have used massively parallel gene sequencing (MPS) to determine the genetic basis of a dominant form of limb girdle muscular dystrophy in affected individuals from seven unrelated families.[Results] The c.700G> A, [p.(Gly234Arg)], c.1327T> C [p.(Ser443Pro], c.1333G> A [p.(Gly445Arg)], c.1661A> C [p.(Tyr554Ser)] and c.1706T> C [p.(Phe569Ser)] CAPN3 variants were identified. Affected individuals presented in young adulthood with progressive proximal and axial weakness, waddling walking and scapular winging or with isolated hyperCKaemia. Muscle imaging showed fatty replacement of paraspinal muscles, variable degrees of involvement of the gluteal muscles, and the posterior compartment of the thigh and minor changes at the mid-leg level. Muscle biopsies revealed mild myopathic changes. Western blot analysis revealed a clear reduction in calpain 3 in skeletal muscle relative to controls. Protein modelling of these variants on the predicted structure of calpain 3 revealed that all variants are located in proximity to the calmodulin-binding site and are predicted to interfere with proteolytic activation.[Conclusions] We expand the genotypic spectrum of CAPN3-associated muscular dystrophy due to autosomal dominant missense variants.This study was funded in part by Instituto de Salud Carlos III through the project PI14/00738 to M. O. (co-funded by European Regional Development Fund. ERDF, a way to build Europe). We thank CERCA Programme / Generalitat de Catalunya for institutional support NGL (APP1117510) and GR (APP1122952) are supported by the Australian National Health and Medical Research Council (NHMRC). This work is also funded by an NHMRC Project Grant (APP1080587).Peer reviewe