The healing effects of the topical mesenchymal stem cells application on colonic anastomosis subjected to ischemia reperfusion injury

Intestinal ischemia reperfusion injury (IRI) is a challenging problem and it adversely affects the healing of colonic anastomosis. Our experimental study aimed to investigate the role of mesenchymal stem cells (MSC) administration in the healing of colonic anastomosis. A total of 33 rats were grouped as Control, IRI and MSC treatment groups. Three rats were reserved for obtaining MSCs. Colonic resection and anastomosis procedure was performed in all groups. Anastomotic line was wrapped with MSCs impregnated spongostan after colonic anastomosis in the rats of the MSC treatment group. All rats were sacrificed and anastomotic line were sampled for examination on the post operative seventh day. Tissue hydroxyproline (HP) levels and anastomotic bursting pressures were statistically compared. Anastomotic bursting pressures were found to be significantly high in MSC treatment group rats. The lowest anastomotic bursting pressure was detected in IRI group rats. Hydroxyproline content of the anastomotic sites were also found to be significantly higher in the rats of the MSC treatment group when compared with the IRI group rats. Our study showed that the detrimental effects of IRI on the healing process of colonic anastomosis in an experimental model may be alleviated with the treatment of MSCs. © 2021, Veteriner Fakultesi Dergisi. All rights reserved

Razlike u vremenu provedenom ispred ekrana između sveučilišnih studenata u Turskoj kao pokazatelj sjedilačkog stila života i neaktivnosti

The aim of the present study was to examine the screen time differences as an indicator of sedentary lifestyle in terms of some socio-demographic variables among university students. A total of 2209 university student (Mage = 20.80, SD = 1.61 years) participated in this study. The demographic information and screen time data were collected by using a survey form. The screen time refers to the combined time spent on watching TV/video, watching/playing/working on a computer, and playing video games. Two x three factorial ANOVA revealed significant gender and socio-economic status differences in the screen time. The male students and those with a high socio-economic status had higher screen time. In addition, one-way ANOVA showed significant screen time differences in terms of parental education level and place of residence (p < .001). The students living in dormitories and those whose parents had lower education level had lower screen time. Also, the students who had an opportunity to access technological devices in their place of residence and in their bedrooms had higher screen time than the students who did not have these facilities (p<.001). The screen time as a sedentary behavior among university students is quite high and these findings can be taken into account as a warning indicating increases in the sedentary lifestyle of these young adults.Cilj istraživanja bio je utvrditi, na uzorku sveučilišnih studenata, razlike u vremenu provedenom ispred ekrana kao pokazatelju sjedilačkog stila života s obzirom na neke društveno-demografske varijable. U istraživanju je sudjelovalo ukupno 2209 sveučilišnih studenata (Mdob = 20,80, SD = 1,61 godina). Demografski podaci i podaci o vremenu provedenom ispred ekrana prikupljeni su s pomoću anketnog obrasca. Vrijeme provedeno ispred ekrana odnosi se na kombinaciju vremena provedenog gledajući televiziju/video, gledajući računalo/igrajući se/radeći na njemu, te igrajući videoigre. 2 x 3 faktorska ANOVA otkrila je značajne razlike u vremenu provedenom ispred ekrana prema spolu i društveno-ekonomskom statusu. Kod studenata muškog spola i onih s visokim društveno-ekonomskim statusom zabilježene su više vrijednosti. Jednosmjerna ANOVA dodatno je pokazala značajne razlike s obzirom na obrazovanje roditelja i mjesto stanovanja (p < ,001). Studenti u studentskim domovima i oni čiji su roditelji na niskoj obrazovnoj razini imaju niže vrijednosti za vrijeme provedeno ispred ekrana. Također, studenti koji imaju mogućnost pristupa tehnološkim uređajima u svom domu i vlastitoj sobi imaju više vrijednosti od onih koji nemaju takve mogućnosti (p<,001). Vrijednost zabilježena za vrijeme provedeno ispred ekrana kao pokazatelj sjedilačkog ponašanja kod sveučilišnih studenata sasvim je visoka, pa se rezultati mogu uzeti u obzir kao upozorenje koje ukazuje na uzlazni trend s obzirom na sjedilački stil života mladih odraslih

Healing effects of single-dose triptolide in rats with severe acute pancreatitis

Aim: Severe acute pancreatitis (SAP) carries high morbidity and mortality risk. If theproinflammatory response phase of SAP cannot be controlled, it may result in multiorganfailure (MOF). Nuclear factor-kappa B (NF-?B) activation plays an important role in thedevelopment of MOF. In this study, it was aimed to investigate the healing effects of triptolide,an anti-inflammatory and immunosuppressive agent in rats with SAP.Material and Methods: A total of 20 Wistar-Albino rats were divided into two groups as theSAP and triptolide treatment (TT) groups. SAP was induced by intraperitoneal injection ofcerulean (50 mg/kg) in both groups. TT group was administered a single dose (0.2 mg/kg)triptolide 24 hour after the induction of SAP. Serum ALT, AST, GGT, Lipase, Glucose, ALPand amylase levels and pancreatic tissue samples were examined.Results: Serum glucose and amylase levels were found to be significantly lower in the TTgroup (p=0.011 and p=0.035, respectively). There was no significant difference between thegroups in terms of other biochemical parameters. Pancreatic edema, acinar cell degeneration,fat necrosis, intrapancreatic&perivascular inflammation, inflammation in the peripancreatic fattissue were common histopathological findings in both groups. There was no significantdifference between the groups in terms of histopathologic changes.Conclusion: Cerulein-induced pancreatitis is a successful method for experimental SAP. Thehealing effects of single-dose triptolide treatment are not evident in the early phase of SAP.The therapeutic effects of triptolide on inflammatory and oxidative stress were not significantlyapproved by histopathological and biochemical parameters by the pancreatic tissue

Investigation of the Interobserver Variability of the Prostate Imaging for Recurrence Reporting (PI-RR) Score in Evaluating Local Recurrence in Prostate Cancer After Radiotherapy: Are Tumor Size and Prostate-specific Antigen Important?

INTRODUCTION: The aim of the study was to investigate the interobserver variability of the newly developed Prostate Imaging for Relapse Reporting (PI-RR) to assess recurrence in patients with prostate cancer who received radiotherapy. METHODS: A total of 81 patients who underwent multiparametric magnetic resonance imaging (mpMRI) examination between January 2018 and November 2022 due to the suspicion of local recurrence were retrospectively evaluated. All the mpMRI images were independently reviewed by three radiologists. None of the observers were blinded to clinical and pathological data (including primary tumor location). It was examined whether interobserver agreement increased according to the prostate-specific antigen (PSA) cut-off value being taken as 0,5 or 0,6 (µg/L) and the size of the tissue with tumor suspicion being greater than 1 cm or 1,5 cm. RESULTS: The mean age of the patients was 68,3 +- 6,6 years. The mean PSA was 2,32 +- 2,95 (µg/L), and the mean tumor size was 1,23 +- 0,77 (cm). A very high level of agreement was detected between the gold standard and observers 1, 2, and 3 (&#954;: 0,921 / 0,873 / 0,825, respectively). The agreement between the observers and the gold standard was also increased in the evaluation of patients with a PSA value above 0,5 (µg/L). Furthermore, patients with a tumor size greater than 1 cm had a very high level of agreement between the gold standard and the observers. DISCUSSION AND CONCLUSION: PI-RR detection rate of relapse tissue and interobserver agreement were correlated with PSA level and size of relapse tissue

Congenital arterial thrombosis in newborn: A case report

Neonatal thrombosis is a serious event that can cause mortality or severe morbidity. Although catheters are the most common cause of neonatal thrombosis, spontaneous events can also occur. Arterial thrombosis is very rare and accounts for approximately half of all thrombotic events in neonates. Genetic prothrombotic risk factors may affect the occurence of neonatal thrombosis. In this report, a case of left brachial, radial, and ulnar arterial thrombosis associated with methylene-tetrahydrofolate reductase (MTHFR) gene C677T and A1298C polymorphism heterozygosity is presented. Plasma homocysteine level and other prothrombotic components were normal. Standard heparin, aspirin, vitamin B12, B6 and folic acid were initiated for treatment. However, the left arm of the patient was amputated at the shoulder because its capillary stream could not be observed. We suggest that MTHFR gene C677T and A1298C polymorphism heterozygosity might be investigated in neonates with congenital arterial thrombosis in spite of normal serum homocysteine levels. © Trakya University Faculty of Medicine

INCIDENTAL GALLBLADDER CANCERS BETWEEN 2013-2017 YEARS IN DEPARTMENT OF GENERAL SURGERY OF KIRIKKALE UNIVERSITY MEDICAL FACULTY

AMAÇ: Safra kesesi tümörleri sıklıkla yaşlılarda görülen agresif seyirli tümörlerdir. Genellikle ilerlemiş halde tanı konabilen safra kesesi tümörlerine erken dönemde kolesistektomi yapılmaksızın tanı konulması oldukça zordur. Tüm gastrointestinal sistem maligniteleri içinde 5. Sıklıkta görülen safra kesesi tümörlerinin tüm evrelerdeki safra kesesi tümörleri göz önüne alındığında tanı sonrası 5 yıllık yaşam oranı %5 olarak görülmektedir. GEREÇ VE YÖNTEMLER: Bu çalışmada Kırıkkale Üniversitesi Tıp Fakültesi Genel Cerrahi Anabilim Dalı'nda 2013 -2017 tarihleri arasında taşlı veya taşsız akut ve kronik kolesistit tanısıyla ameliyat edilen 867 hastanın histopatolojik sonuçları incelenmiştir. BULGULAR: Bu dönemde 867 hasta içinde 7 malignite olgusu saptanmış olup 6 tanesi adenokarsinom, 1 tanesi nöroendokrin tümör tanısı almıştır. Serimizde malignite oranı % 0,8 olarak bulunmuştur.SONUÇ: Maligniteden ve bilier komplikasyonlardan kaçınmak için safra kesesi taşlarına erken ameliyat önerilebileceğini düşünmekteyiz.OBJECTIVE: Gallbladder cancer is an aggressive malignancy that is more frequently seen in the elderly. Diagnosis of gallbladder cancers at early stages is almost impossible without cholecystectomy, thus these cancers are usually diagnosed at an advanced stage. Gallbladder cancers are 5th among malignancies of biliary system. When all stages of gallbladder cancers are encountered 5 year survival is only 5%. MATERIAL AND METHODS: The histopathologic results of 867 patients that underwent cholecystectomy in Department of Surgery of Kirikkale University Medical Faculty between 2013 and 2017 with diagnoses of chronic and acute calculous cholecystitis as well as acute acalculous cholecystitis were evaluated retrospectively.RESULTS: Seven malignancies were diagnosed among 867 patients. Six of the patients were diagnosed with adenocarcinoma of the gallbladder while histopathological examination revealed neuroendocrine tumor in 1 patient. The incidence of malignancy was found to be 0,8% in this series. CONCLUSION: Cholecytstectomy for calculous cholecystitis can be offered earlier to patients in order to avoid late on-set gallbladder cancer and biliary complications

Aktievärderingsmodeller : Vågar man lita på dem?

Purpose: The purpose of this essay is to apply "Discount Dividend model" and "Discounted Cash Flow model" in reality during periods of economic boom alternatively recession. Method: A quantitative research method as well as secondary data and data collection method is used. This data consists of the company’s annual reports. Totally, 20 companies of different sizes under diverse economic conditions are involved in this data. Conclusion: After reviewing the companies’ calculations, it is noted that the both models that are used reflect better stock-exchange rate during an economic recession

The Effects of Flaxseed Oil on Cadmium-Induced Oxidative Stress in Rats

In the present study, the effects of flaxseed oil on the oxidant-antioxidant system in cadmium intoxication were investigated in rats. Forty-eight male Wistar albino rats were divided into four equal groups (group 1). No treatment was applied to the control group. On the other hand, groups 2, 3, and 4 were administered with 0.1 ml/rat/day (similar to 500 mg/kg bw) flaxseed oil by gavage into the stomach, 50 ppm of cadmium (similar to 4 mg/kg bw) in ad libitum drinking water, and 0.1 ml/rat/day flaxseed oil plus 50 ppm of cadmium, respectively, for 30 days. At the end of the study, malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase activities were measured in blood and tissue (liver, lung, kidney, brain, heart, and testes) samples. While malondialdehyde and nitric oxide levels increased in the group given cadmium compared to the control group; in the meantime, there were some significant changes in antioxidant enzyme activities. These changes were observed, the trends of decrease or increase compared to the control group. There were positive changes in parameters of the group given with flaxseed oil plus cadmium compared to the group receiving cadmium alone, in other words, values were seen coming close to control group. As a result, cadmium exposure caused oxidative damage to erythrocytes and organs at varying rates, while flaxseed oil reduced the severity of cadmium-induced lipid peroxidation. Therefore, it was concluded that flaxseed oil can be used among compounds as a therapeutic agent or food additive for prophylaxis in cadmium intoxication. In the present study, the effects of flaxseed oil on the oxidant-antioxidant system in cadmium intoxication were investigated in rats. Forty-eight male Wistar albino rats were divided into four equal groups (group 1). No treatment was applied to the control group. On the other hand, groups 2, 3, and 4 were administered with 0.1&nbsp;ml/rat/day (&sim;500&nbsp;mg/kg bw) flaxseed oil by gavage into the stomach, 50&nbsp;ppm of cadmium (&sim;4&nbsp;mg/kg bw) in ad libitum drinking water, and 0.1&nbsp;ml/rat/day flaxseed oil plus 50&nbsp;ppm of cadmium, respectively, for 30&nbsp;days. At the end of the study, malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase activities were measured in blood and tissue (liver, lung, kidney, brain, heart, and testes) samples. While malondialdehyde and nitric oxide levels increased in the group given cadmium compared to the control group; in the meantime, there were some significant changes in antioxidant enzyme activities. These changes were observed, the trends of decrease or increase compared to the control group. There were positive changes in parameters of the group given with flaxseed oil plus cadmium compared to the group receiving cadmium alone, in other words, values were seen coming close to control group. As a result, cadmium exposure caused oxidative damage to erythrocytes and organs at varying rates, while flaxseed oil reduced the severity of cadmium-induced lipid peroxidation. Therefore, it was concluded that flaxseed oil can be used among compounds as a therapeutic agent or food additive for prophylaxis in cadmium intoxication.</p