Étude du rôle du médecin généraliste dans la réduction de l'affectivité négative (analyse qualitative à partir du discours des patients)

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Médecine (341722104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Genotyping by Sequencing Using Specific Allelic Capture to Build a High-Density Genetic Map of Durum Wheat.

Targeted sequence capture is a promising technology which helps reduce costs for sequencing and genotyping numerous genomic regions in large sets of individuals. Bait sequences are designed to capture specific alleles previously discovered in parents or reference populations. We studied a set of 135 RILs originating from a cross between an emmer cultivar (Dic2) and a recent durum elite cultivar (Silur). Six thousand sequence baits were designed to target Dic2 vs. Silur polymorphisms discovered in a previous RNAseq study. These baits were exposed to genomic DNA of the RIL population. Eighty percent of the targeted SNPs were recovered, 65% of which were of high quality and coverage. The final high density genetic map consisted of more than 3,000 markers, whose genetic and physical mapping were consistent with those obtained with large arrays

Polymorphisms available for bait design.

<p>Polymorphisms available for bait design.</p

Description of the bait design.

<p>Orange lines represent the durum wheat genome, with the targeted SNP in brackets. Bait sequences are represented in grey. The number of SNPs targeted by each type of bait is specified.</p

Efficiency of the genotyping by capture protocol.

<p>Efficiency of the genotyping by capture protocol.</p

Features of the DS durum wheat genetic map.

<p>Features of the DS durum wheat genetic map.</p

Correlations between putative physical and genetic positions.

<p>The 14 durum wheat chromosomes are shown separately, with the putative physical position on the X-axis (bp) and the genetic position on the Y-axis (cM). The chromosome name is given at the top left of each plot. The number of markers is given in brackets. A red line represents the fitted polynomial model and a grey area represents the 95% confidence interval. The two vertical grey lines are maximum and minimum values of the physical positions.</p

Detection of a bonus SNP on the homeolog of a targeted contig.

<p>A portion of A and B genomes were represented, with an SNP on the A genome (in red) and an SNP on the B genome (in blue). Divergences between both genomes are represented in green. The bait shown in grey was designed initially to capture a portion of the A genome, but captured also the homeologous portion of the B genome, with the related bonus SNP.</p

Early human impact on soils and hydro-sedimentary systems: Multi-proxy geoarchaeological analyses from La Narse de la Sauvetat (France)

International audienceWe analysed the late-Holocene pedo-sedimentary archives of La Narse de la Sauvetat, a hydromorphic depression in the southern Limagne plain (central France), where chronologically accurate studies are scarce. The multi-proxy geoarchaeological and palaeoenvironmental analysis of two cores from different areas of the basin was carried out through sedimentological, geochemical, micromorphological and malacological investigations. Integration of these datasets supported by a robust radiocarbon-based chronology allowed discussion of socio-environmental interactions and anthropogenic impacts from Late Neolithic to Early Middle Ages. Until the Middle Bronze Age, there was no clear evidence of anthropogenic impact on soils and hydro-sedimentary dynamics of the catchment, but two peaks of high alluvial activity probably related to the 4.2 and 3.5 kyr. BP climate events were first recorded in Limagne. Significant anthropogenic impacts started in the Late Bronze Age with increased erosion of the surrounding volcanic slopes. However, a major threshold was reached c. 2600 cal BP with a sharp increase in the catchment erosion interpreted as resulting from strong anthropogenic environmental changes related to agricultural activities and drainage. This implies an anthropogenic forcing on soils and hydro-sedimentary systems much earlier than was usually considered in Limagne. These impacts then gradually increased during Late Iron Age and Roman periods, but environmental effects were certainly contained by progress in agricultural management. Late Antiquity environmental changes are consistent with regional trend to drainage deterioration in lowlands, but marked asynchrony in this landscape change suggests that societal factors implying differential land management were certainly predominant here