797 research outputs found
Unusual development of light-reflecting pigment cells in intact and regenerating tail in the periodic albino mutant of Xenopus laevis
Unusual light-reflecting pigment cells, âwhite pigment cellsâ, specifically appear in the periodic albino mutant (ap/ap) of Xenopus laevis and localize in the same place where melanophores normally differentiate in the wild-type. The mechanism responsible for the development of unusual pigment cells is unclear. In this study, white pigment cells in the periodic albino were compared with melanophores in the wild-type, using a cell culture system and a tail-regenerating system. Observations of both intact and cultured cells demonstrate that white pigment cells are unique in (1) showing characteristics of melanophore precursors at various stages of development, (2) accumulating reflecting platelets characteristic of iridophores, and (3) exhibiting pigment dispersion in response to α-melanocyte stimulating hormone (α-MSH) in the same way that melanophores do. When a tadpole tail is amputated, a functionally competent new tail is regenerated. White pigment cells appear in the mutant regenerating tail, whereas melanophores differentiate in the wild-type regenerating tail. White pigment cells in the mutant regenerating tail are essentially similar to melanophores in the wild-type regenerating tail with respect to their localization, number, and response to α-MSH. In addition to white pigment cells, iridophores which are never present in the intact tadpole tail appear specifically in the somites near the amputation level in the mutant regenerating tail. Iridophores are distinct from white pigment cells in size, shape, blue light-induced fluorescence, and response to α-MSH. These findings strongly suggest that white pigment cells in the mutant arise from melanophore precursors and accumulate reflecting platelets characteristic of iridophores
Breakdown of the Two-Step Model in K-Shell Photoemission and Subsequent Decay Probed by the Molecular-Frame Photoelectron Angular Distributions of CO_2
We report results of measurements and of Hartree-Fock level calculations of molecular-frame photoelectron angular distributions (MFPADs) for C 1s photoemission from CO2. The agreement between the measured and calculated MFPADs is on average reasonable. The measured MFPADs display a weak but definite asymmetry with respect to the O+ and CO+ fragment ions at certain energies, providing evidence for an overlap of gerade and ungerade final ionic states giving rise to a partial breakdown of the two-step model of core-level photoionization and its subsequent Auger decay
Excitonic instability and electric-field-induced phase transition towards a two dimensional exciton condensate
We present an InAs-GaSb-based system in which the electric-field tunability
of its 2D energy gap implies a transition towards a thermodynamically stable
excitonic condensed phase. Detailed calculations show a 3 meV BCS-like gap
appearing in a second-order phase transition with electric field. We find this
transition to be very sharp, solely due to exchange interaction, and so, the
exciton binding energy is greatly renormalized even at small condensate
densities. This density gradually increases with external field, thus enabling
the direct probe of the Bose-Einstein to BCS crossover.Comment: LaTex, 11 pages, 3 ps figures, To appear in PR
Drag in paired electron-hole layers
We investigate transresistance effects in electron-hole double layer systems
with an excitonic condensate. Our theory is based on the use of a minimum
dissipation premise to fix the current carried by the condensate. We find that
the drag resistance jumps discontinuously at the condensation temperature and
diverges as the temperature approaches zero.Comment: 12 pages, 1 Figure, .eps file attache
Dissociative photoionization of the NO molecule studied by photoelectron-photon coincidence technique
Low-energy photoelectronâvacuum ultraviolet (VUV) photon coincidences have been measured using synchrotron radiation excitation in the inner-valence region of the nitric oxide molecule. The capabilities of the coincidence set-up were demonstrated by detecting the 2sâ1 â 2pâ1 radiative transitions in coincidence with the 2s photoelectron emission in Ne. In NO, the observed coincidence events are attributed to dissociative photoionization with excitation, whereby photoelectron emission is followed by fragmentation of excited NO+ ions into O+ + N* or N+ + O* and VUV emission from an excited neutral fragment. The highest coincidence rate occurs with the opening of ionization channels which are due to correlation satellites of the 3Ï photoionization. The decay time of VUV photon emission was also measured, implying that specific excited states of N atoms contribute significantly to observed VUV emission
Germline mutations and somatic inactivation of TRIM28 in Wilms tumour
© 2018 Halliday et al. http://creativecommons.org/licenses/by/4.0/ Wilms tumour is a childhood tumour that arises as a consequence of somatic and rare germline mutations, the characterisation of which has refined our understanding of nephrogenesis and carcinogenesis. Here we report that germline loss of function mutations in TRIM28 predispose children to Wilms tumour. Loss of function of this transcriptional co-repressor, which has a role in nephrogenesis, has not previously been associated with cancer. Inactivation of TRIM28, either germline or somatic, occurred through inactivating mutations, loss of heterozygosity or epigenetic silencing. TRIM28-mutated tumours had a monomorphic epithelial histology that is uncommon for Wilms tumour. Critically, these tumours were negative for TRIM28 immunohistochemical staining whereas the epithelial component in normal tissue and other Wilms tumours stained positively. These data, together with a characteristic gene expression profile, suggest that inactivation of TRIM28 provides the molecular basis for defining a previously described subtype of Wilms tumour, that has early age of onset and excellent prognosis
Temporal blastemal cell gene expression analysis in the kidney reveals new Wnt and related signaling pathway genes to be essential for Wilms' tumor onset
Wilms' tumors (WTs) originate from metanephric blastema cells that are unable to complete differentiation, resulting in triphasic tumors composed of epithelial, stromal and blastemal cells, with the latter harboring molecular characteristics similar to those of the earliest kidney development stages. Precise regulation of Wnt and related signaling pathways has been shown to be crucial for correct kidney differentiation. In this study, the gene expression profile of Wnt and related pathways was assessed in laser-microdissected blastemal cells in WTs and differentiated kidneys, in human and in four temporal kidney differentiation stages (i.e. E15.5, E17.5, P1.5 and P7.5) in mice, using an orthologous cDNA microarray platform. A signaling pathway-based gene signature was shared between cells of WT and of earliest kidney differentiation stages, revealing genes involved in the interruption of blastemal cell differentiation in WT. Reverse transcription-quantitative PCR showed high robustness of the microarray data demonstrating 75 and 56% agreement in the initial and independent sample sets, respectively. The protein expression of CRABP2, IGF2, GRK7, TESK1, HDGF, WNT5B, FZD2 and TIMP3 was characterized in WTs and in a panel of human fetal kidneys displaying remarkable aspects of differentiation, which was recapitulated in the tumor. Taken together, this study reveals new genes candidate for triggering WT onset and for therapeutic treatment targets
Photoelectron Angular Distributions for Two-photon Ionization of Helium by Ultrashort Extreme Ultraviolet Free Electron Laser Pulses
Phase-shift differences and amplitude ratios of the outgoing and
continuum wave packets generated by two-photon ionization of helium atoms are
determined from the photoelectron angular distributions obtained using velocity
map imaging. Helium atoms are ionized with ultrashort extreme-ultraviolet
free-electron laser pulses with a photon energy of 20.3, 21.3, 23.0, and 24.3
eV, produced by the SPring-8 Compact SASE Source test accelerator. The measured
values of the phase-shift differences are distinct from scattering phase-shift
differences when the photon energy is tuned to an excited level or Rydberg
manifold. The difference stems from the competition between resonant and
non-resonant paths in two-photon ionization by ultrashort pulses. Since the
competition can be controlled in principle by the pulse shape, the present
results illustrate a new way to tailor the continuum wave packet.Comment: 5 pages, 1 table, 3 figure
Ring-shaped spatial pattern of exciton luminescence formed due to the hot carrier transport in a locally photoexcited electron-hole bilayer
A consistent explanation of the formation of a ring-shaped pattern of exciton
luminescence in GaAs/AlGaAs double quantum wells is suggested. The pattern
consists of two concentric rings around the laser excitation spot. It is shown
that the luminescence rings appear due to the in-layer transport of hot charge
carriers at high photoexcitation intensity. Interestingly, one of two causes of
this transport might involve self-organized criticality (SOC) that would be the
first case of the SOC observation in semiconductor physics. We test this cause
in a many-body numerical model by performing extensive molecular dynamics
simulations. The results show good agreement with experiments. Moreover, the
simulations have enabled us to identify the particular kinetic processes
underlying the formation of each of these two luminescence rings.Comment: 14 pages, 16 figures. Final versio
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