Antithrombotic/anticoagulant and anticancer activities of selected medicinal plants from South Africa

Nine plants available in the Eastern Cape Province of South Africa were tested for antithrombotic and/or anticoagulant activity. Organic (methanol) and aqueous (distilled water) extractions were performed onthe various plant parts. The thrombin assay and clotting time assays (thrombin-induced and CaCl2-induced) were utilised. Several extracts displayed activity, but in most cases this was due to the presence of tannins. Only the aqueous extracts displayed activity after tannin removal. The Sutherlandia frutescens leaf extract displayed antithrombotic activity, with an IC50 value of 2.17 mg/ml. Gloriosa superba and Zantedeschia aethiopica leaf extracts displayed anticoagulant properties by inhibiting thrombin-induced clotting, with IC50 values of 2.97 and 3.05 mg/ml, respectively. The Leonotisleonurus root extract was found to decrease the CaCl2-induced clotting time by 50% at 8.88 mg/ml. A decrease in this value accompanied by a decrease in fibrin formation was preferable for the CaCl2-induced assay, since decreased fibrin formation may have a role in the prevention of cancer metastasis. As tannins were found to contribute minimally to the anticoagulant effect of L. leonurus, the cytotoxicity potential of the extracts of this species against five cell lines was determined. Only the organic extract yielded significant cytotoxity

Containerless processing of beryllium

Melting and solidification of a beryllium alloy containing 1.5% BeO by weight in the weightless environment of space has produced cast beryllium with a relatively uniform dispersion of BeO throughout. Examination of the cast material shows that it is coarse grained, although the BeO is not heavily agglomerated in the flight specimen. Ground based comparison experiments show extreme agglomeration and segregation of BeO, resulting in large zones which are practically free of the oxide. Several postulated hypotheses for the failure to grain refine the beryllium are formulated. These are: (1) spherodization of the BeO particles during specimen preparation and during the molten phase of the experiment; (2) loss of nucleation potency through aging in the molten phase; and (3) inability of BeO to act as a grain refiner for beryllium. Further investigation with non spherodized particles and shorter dwell times molten may delineate which of these hypotheses are valid. The results of this flight experiment indicate that the weightless environment of space is an important asset in conducting research to find grain refiners for beryllium and other metals for which cast dispersions of grain refining agents cannot be prepared terrestrially due to gravitationally driven settling and agglomeration

MWC 656 is unlikely to contain a black hole

Context. MWC 656 was reported as the first known Be star with a black-hole (BH) companion in a 60 d period. The mass of the proposed BH companion is estimated to be between 4 - 7 MSun. This estimate is based on radial velocity (RV) measurements derived from the Fe ii 4583 emission line of the Be star disc and from the He ii 4686 emission line, assumed to be formed in a disc around the putative BH. Aims. Using new high-resolution spectroscopic data, we investigate whether MWC 656 truly contains a BH. Methods. We used the cross-correlation method to calculate the RVs of both the Be star and the He ii 4686 emission line and we derive a new orbital solution. We also performed disentangling to look for the spectral signature of a companion. Results. We derive an orbital period of 59.028 +- 0.011 d and a mass ratio q = M_Heii/M_Be = 0.12 +- 0.03, much lower than the previously reported q = 0.41 +- 0.07. Adopting a mass of the Be star of M_Be = 7.8 +- 2.0MSun, the companion has a mass of 0.94 +- 0.34MSun. For the upper limit of M_Be = 16MSun and q = 0.15, the companion has a mass 2.4MSun. Performing disentangling on mock spectra shows that the spectral signature of a non-degenerate stellar companion with such a low mass cannot be retrieved using our data. Conclusions. Our measurements do not support the presence of a BH companion in MWC 656. The derived upper limit on the mass of the companion rather indicates that it is a neutron star, a white dwarf, or a hot helium star. Far-UV data will help to reject or confirm a hot helium-star companion.Comment: 6 pages, 2 + 6 figures, 3 + 1 tables, accepted for publication in A&

Nonlinear Dynamics of Composite Fermions in Nanostructures

We outline a theory describing the quasi-classical dynamics of composite fermions in the fractional quantum Hall regime in the potentials of arbitrary nanostructures. By an appropriate parametrization of time we show that their trajectories are independent of their mass and dispersion. This allows to study the dynamics in terms of an effective Hamiltonian although the actual dispersion is as yet unknown. The applicability of the theory is verified in the case of antidot arrays where it explains details of magnetoresistance measurements and thus confirms the existence of these quasiparticles.Comment: submitted to Europhys. Lett., 4 pages, postscrip

Explicitly correlated trial wave functions in Quantum Monte Carlo calculations of excited states of Be and Be-

We present a new form of explicitly correlated wave function whose parameters are mainly linear, to circumvent the problem of the optimization of a large number of non-linear parameters usually encountered with basis sets of explicitly correlated wave functions. With this trial wave function we succeeded in minimizing the energy instead of the variance of the local energy, as is more common in quantum Monte Carlo methods. We applied this wave function to the calculation of the energies of Be 3P (1s22p2) and Be- 4So (1s22p3) by variational and diffusion Monte Carlo methods. The results compare favorably with those obtained by different types of explicitly correlated trial wave functions already described in the literature. The energies obtained are improved with respect to the best variational ones found in literature, and within one standard deviation from the estimated non-relativistic limitsComment: 19 pages, no figures, submitted to J. Phys.

Diametral compression test method to analyse relative surface stresses in thermally sprayed coated and uncoated circular disc specimens.

In firsts of its investigation, a diametral compression destructive testing method (also known as Brazilian test) was performed on thermally sprayed coated and uncoated circular disc specimens to compare relative surface stresses. The coating investigated had about 250 μm thickness deposited on 4.76 mm thick Hastelloy®X substrate discs of 20 mm diameter. In the instrumented experiment (diametral compression test) strain gauge rosettes were used to measure strains on two circular surfaces of disc specimen (coated and uncoated sides) and converted to stress values for analysis. Where comparisons were made, the experimental and finite element simulation results were in some agreement with overall understanding of the diametral compression testing behaviour. For coated specimen, test results convey that higher stresses exist within the uncoated side of the specimen rather than the coated side. Although the methods proposed would be deemed most comparable to real life scenarios (e.g. to quantify coating delamination strength and failure mechanics), this type of experimental investigation has certain advantages and limitations

Understanding surgical antimicrobial prescribing behaviour in the hospital setting: a systematic review and meta-ethnography protocol.

This is the final version. Available from BMC via the DOI in this record. Availability of data and materials: Not applicable.BACKGROUND: Surgical specialities use extensive amounts of antimicrobials, and misuse has been widely reported, making them a key target for antimicrobial stewardship initiatives. Interventions informed by, and tailored to, a clear understanding of the contextual barriers to appropriate antimicrobial use are more likely to successfully improve practice. However, this approach has been under utilised. Our aim is to synthesise qualitative studies on surgical antimicrobial prescribing behaviour (APB) in hospital settings to explain how and why contextual factors act and interact to influence APB amongst surgical teams. We will develop new theory to advance understanding and identify knowledge gaps to inform further research. METHODS: The meta-ethnography will follow the seven-phase method described by Noblit and Hare. We will conduct a comprehensive search using eight databases (AMED, CINAHL, EMBASE, MEDLINE, MEDLINE-in-process, Web of Science, Cochrane Library and PsycINFO) with no date restrictions; forwards and backwards citation searches; and contacting first authors of relevant papers. Studies will be dual screened and included if they use recognised qualitative methods and analysis; focus on contextual factors associated with surgical APB within hospital settings; are available in full in English; and are relevant to the research question. Any disagreements between reviewers will be resolved through discussion to reach consensus. Included studies will be read repeatedly to illuminate key concepts and the relationship between key concepts across studies. Then, key concepts will be sorted into conceptual categories or 'piles' which will be further abstracted to form a conceptual framework explaining surgical APB. During the synthesis, emerging interpretations will be discussed with stakeholders (including authors of included studies where possible; surgical and stewardship practitioners; and patient representatives) to ensure new knowledge is meaningful. DISCUSSION: This research has several strengths: (1) the protocol has been written with reference to established guidance maximising rigour and transparency; (2) the multi-disciplinary research team bring varied interpretative repertoires and relevant methodological skills; and (3) stakeholders will be involved to ensure that findings are relevant, and disseminated via suitable channels, to support improved patient care. SYSTEMATIC REVIEW REGISTRATION: PROSPERO CRD42020184343.Health Education England/National Institute for Health Researc