Microencapsulation As A Potential Control Technique Against Sabellid Worms In Abalone Culture

We have developed a novel application for lipid-walled microcapsules (LWMs) in the potential control of sabellid infestations in abalone aquaculture. The use of LWMs takes advantage of the filter-feeding nature of the worms, versus the herbivory of the host abalone. Initial observations indicated that the pest was capable of feeding on particles ranging from 3-30 mu m in size. Lipid-walled microcapsules were prepared using different combinations of lipids (tristearin, tripalmitin, and fish oil) to encapsulate water-based solutions. Feeding experiments using worm-infested shells indicated that in a relatively short time (30-60 min) most of the worms (80-95%) fed on the LWMs and that large numbers of LWMs were ingested. Fecal pellets containing LWMs were observed in the rectums of worms within 15-30 min. Feeding efficiency was examined using different concentrations of LWMs. The sabellid worm was an efficient feeder. At low particle densities (2.6 x 10(4) particles/mL), 66.7% of the worms had eaten modest levels of LWMs. An asymptote in particle density in relation to feeding occurred at 2.6 x 10(5) particles/mL, with 83% of the worms feeding on large numbers of particles. In separate observations, LWMs composed of tripalmitin and fish oil were observed in various stages of digestion in the stomach, rectum, and fecal pellets of the worms. Microcapsules were also observed in the digestive tract of mud worms, Polydora spp. that were also inhabiting abalone shell. The utility of LWMs for delivery of toxins to the sabellid pest holds much promise in ridding the industry of this nuisance species

Transmission Of Withering Syndrome In Black Abalone, Haliotis Cracherodii Leach

Withering syndrome (WS) has been associated with catastrophic declines in black abalone populations in southern and central California. In an effort to identify the etiological agent of WS and to characterize the progression of this disease, we initiated a transmission study in which abalone from Ano Nuevo Island, a location free of WS, shared aquaria with animals from Vandenberg Airforce Base, a location where WS is epizootic. The mean incubation period of WS (time to develop overt signs of the disease) was 245 days with a mean time to death after development of clinical signs of 42 days. Median time to death was 41 wk in the experimentally exposed Ano Nuevo Island abalone and 16 wk in the positive control Vandenberg abalone. Cumulative mortality was significantly different between the negative control (unexposed) Ano Nuevo Island abalone (25% mortality) and both the exposed Ano Nuevo Island abalone (85% mortality; P = 0.0001) and the positive control Vandenberg abalone (100% mortality; P = 0.0001). In addition, significant differences in prevalences of a recently described Rickettsiales-like procaryote (RLP), Candidatus Xenohaliotis californiensis, were observed between negative control animals (no RLPs) and those with WS (both the experimentally exposed Ano Nuevo Island and Vandenberg abalone were infected with RLPs; P \u3c 0.001). All abalone infected with the RLP had signs of WS, including decreased condition indices, foot muscle atrophy, and digestive gland degeneration (P \u3c 0.05). No correlation between intensity of RLP infection and degree of WS was observed (P \u3e 0.05), suggesting a complex relationship between the RLP and clinical disease in black abalone. Despite this, these data in conjunction with a lack of observation of any other significant pathogens in the abalone provides evidence that the RLP infecting abalone ( Candidatus Xenohaliotis californiensis ) is the etiological agent of WS

\u27Candidatus Xenohaliotis californiensis\u27, a newly described pathogen of abalone, Haliotis spp., along the west coast of North America

Withering syndrome is a fatal disease of wild and cultured abalone. Haliotis spp., that inhabit the west coast of North America. The aetiological agent of withering syndrome has recently been identified as a member of the family Rickettsiaceae in the order Rickettsiales. Using a combination of morphological, serological, life history and genomic (16S rDNA) characterization. we have identified this bacterium as a unique taxon and propose the provisional status of \u27Candidatus Xenohaliotis californiensis\u27, The Gram-negative. obligate intracellular pleomorphic bacterium is found within membrane-bound vacuoles in the cytoplasm of abalone gastrointestinal epithelial cells. The bacterium is not cultivable on synthetic media or in fish cell lines (e.g. CHSE-214) and may be controlled by tetracyclines (oxytetracycline) but not by chloramphenicol, clarithromycin or sarafloxicin. Phylogenetic analysis based on the 16S rDNA of \u27Candidatus Xenohaliotis californiensis\u27 places it in the alpha-subclass of the class Proteobacteria but not to the four recognized subtaxa of the alpha-Proteobacteria (alpha-1, alpha-2, alpha-3 and alpha-4). The bacterium can be detected in tissue squashes stained with propidium iodide, microscopic examination of stained tissue sections, PCR or in situ hybridization. \u27Candidatus Xenohaliotis californiensis\u27 can be differentiated from other closely related alpha-Proteobacteria by its unique 16S rDNA sequence

A Brownian particle in a microscopic periodic potential

We study a model for a massive test particle in a microscopic periodic potential and interacting with a reservoir of light particles. In the regime considered, the fluctuations in the test particle's momentum resulting from collisions typically outweigh the shifts in momentum generated by the periodic force, and so the force is effectively a perturbative contribution. The mathematical starting point is an idealized reduced dynamics for the test particle given by a linear Boltzmann equation. In the limit that the mass ratio of a single reservoir particle to the test particle tends to zero, we show that there is convergence to the Ornstein-Uhlenbeck process under the standard normalizations for the test particle variables. Our analysis is primarily directed towards bounding the perturbative effect of the periodic potential on the particle's momentum.Comment: 60 pages. We reorganized the article and made a few simplifications of the conten

Can stellar mass black holes be quark stars?

We investigate the possibility that stellar mass black holes, with masses in the range of $3.8M_{\odot}$ and $6M_{\odot}$, respectively, could be in fact quark stars in the Color-Flavor-Locked (CFL) phase. Depending on the value of the gap parameter, rapidly rotating CFL quark stars can achieve much higher masses than standard neutron stars, thus making them possible stellar mass black hole candidates. Moreover, quark stars have a very low luminosity and a completely absorbing surface - the infalling matter on the surface of the quark star is converted into quark matter. A possibility of distinguishing CFL quark stars from stellar mass black holes could be through the study of thin accretion disks around rapidly rotating quark stars and Kerr type black holes, respectively. Furthermore, we show that the radiation properties of accretion disks around black holes and CFL quark stars are also very similar. However, strange stars exhibit a low luminosity, but high temperature bremsstrahlung spectrum, which, in combination with the emission properties of the accretion disk, may be the key signature to differentiate massive strange stars from black hole.Comment: 27 pages, 5 figures, accepted for publication in MNRA

The Hubble Constant

I review the current state of determinations of the Hubble constant, which gives the length scale of the Universe by relating the expansion velocity of objects to their distance. There are two broad categories of measurements. The first uses individual astrophysical objects which have some property that allows their intrinsic luminosity or size to be determined, or allows the determination of their distance by geometric means. The second category comprises the use of all-sky cosmic microwave background, or correlations between large samples of galaxies, to determine information about the geometry of the Universe and hence the Hubble constant, typically in a combination with other cosmological parameters. Many, but not all, object-based measurements give $H_0$ values of around 72-74km/s/Mpc , with typical errors of 2-3km/s/Mpc. This is in mild discrepancy with CMB-based measurements, in particular those from the Planck satellite, which give values of 67-68km/s/Mpc and typical errors of 1-2km/s/Mpc. The size of the remaining systematics indicate that accuracy rather than precision is the remaining problem in a good determination of the Hubble constant. Whether a discrepancy exists, and whether new physics is needed to resolve it, depends on details of the systematics of the object-based methods, and also on the assumptions about other cosmological parameters and which datasets are combined in the case of the all-sky methods.Comment: Extensively revised and updated since the 2007 version: accepted by Living Reviews in Relativity as a major (2014) update of LRR 10, 4, 200

Simpson's Paradox, Lord's Paradox, and Suppression Effects are the same phenomenon – the reversal paradox

This article discusses three statistical paradoxes that pervade epidemiological research: Simpson's paradox, Lord's paradox, and suppression. These paradoxes have important implications for the interpretation of evidence from observational studies. This article uses hypothetical scenarios to illustrate how the three paradoxes are different manifestations of one phenomenon – the reversal paradox – depending on whether the outcome and explanatory variables are categorical, continuous or a combination of both; this renders the issues and remedies for any one to be similar for all three. Although the three statistical paradoxes occur in different types of variables, they share the same characteristic: the association between two variables can be reversed, diminished, or enhanced when another variable is statistically controlled for. Understanding the concepts and theory behind these paradoxes provides insights into some controversial or contradictory research findings. These paradoxes show that prior knowledge and underlying causal theory play an important role in the statistical modelling of epidemiological data, where incorrect use of statistical models might produce consistent, replicable, yet erroneous results

A primary care, multi-disciplinary disease management program for opioid-treated patients with chronic non-cancer pain and a high burden of psychiatric comorbidity

BACKGROUND: Chronic non-cancer pain is a common problem that is often accompanied by psychiatric comorbidity and disability. The effectiveness of a multi-disciplinary pain management program was tested in a 3 month before and after trial. METHODS: Providers in an academic general medicine clinic referred patients with chronic non-cancer pain for participation in a program that combined the skills of internists, clinical pharmacists, and a psychiatrist. Patients were either receiving opioids or being considered for opioid therapy. The intervention consisted of structured clinical assessments, monthly follow-up, pain contracts, medication titration, and psychiatric consultation. Pain, mood, and function were assessed at baseline and 3 months using the Brief Pain Inventory (BPI), the Center for Epidemiological Studies-Depression Scale scale (CESD) and the Pain Disability Index (PDI). Patients were monitored for substance misuse. RESULTS: Eighty-five patients were enrolled. Mean age was 51 years, 60% were male, 78% were Caucasian, and 93% were receiving opioids. Baseline average pain was 6.5 on an 11 point scale. The average CESD score was 24.0, and the mean PDI score was 47.0. Sixty-three patients (73%) completed 3 month follow-up. Fifteen withdrew from the program after identification of substance misuse. Among those completing 3 month follow-up, the average pain score improved to 5.5 (p = 0.003). The mean PDI score improved to 39.3 (p < 0.001). Mean CESD score was reduced to 18.0 (p < 0.001), and the proportion of depressed patients fell from 79% to 54% (p = 0.003). Substance misuse was identified in 27 patients (32%). CONCLUSIONS: A primary care disease management program improved pain, depression, and disability scores over three months in a cohort of opioid-treated patients with chronic non-cancer pain. Substance misuse and depression were common, and many patients who had substance misuse identified left the program when they were no longer prescribed opioids. Effective care of patients with chronic pain should include rigorous assessment and treatment of these comorbid disorders and intensive efforts to insure follow up

Focal non granulomatous orchitis in a patient with Crohn’s disease

Crohn’s disease is a systemic disease and sometimes involves the testicle, usually leading to granulomatous lesions. We report herein a case of focal non-granulomatous orchitis in a 21-year-old patient with active Crohn’s disease treated by an anti-tumor necrosis factor monoclonal antibody. This circumscribed testicular lesion mimicked a tumor, leading to orchiectomy. Pre-operative blood tests (i.e. alpha-fetoprotein, lactate dehydrogenase and human chorionic gonadotrophin) were strictly normal Pathological examination of the testicle revealed a focal inflammatory infiltrate predominantly composed of lymphocytes accompanied by few plasma cells, lacking giant cells or granulomas. Importantly, intratubular germ cell neoplasia, atrophy or lithiasis were not observed. After discussing and excluding other plausible causes (burnt-out /regressed germ cell tumor, infection, vascular or traumatic lesions, iatrogenic effects), we concluded that this particular case of orchitis was most likely an extra-digestive manifestation of inflammatory bowel disease. To our knowledge, this is the first described case of focal non-granulomatous orchitis associated with Crohn’s disease. Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/211774728416011