Chiral Perturbation Theory for phi -> rho gamma gamma and phi -> omega gamma gamma

We predict differential decay distributions for phi->rho gamma gamma and phi -> omega gamma gamma using chiral perturbation theory. We also consider the isospin violating decay phi -> omega pi^0. Experimental information on these decays can be used to determine couplings in the heavy vector meson chiral Lagrangian.Comment: It was shown by P. Ko et al., hep-ph/9510205 (Phys. Lett. B366 (1996) 287), that there is a etaprime pole contribution that dominates over what we calculate

Isonitrile-responsive and bioorthogonally removable tetrazine protecting groups.

In vivo compatible reactions have a broad range of possible applications in chemical biology and the pharmaceutical sciences. Here we report tetrazines that can be removed by exposure to isonitriles under very mild conditions. Tetrazylmethyl derivatives are easily accessible protecting groups for amines and phenols. The isonitrile-induced removal is rapid and near-quantitative. Intriguingly, the deprotection is especially effective with (trimethylsilyl)methyl isocyanide, and serum albumin can catalyze the elimination under physiological conditions. NMR and computational studies revealed that an imine-tautomerization step is often rate limiting, and the unexpected cleavage of the Si-C bond accelerates this step in the case with (trimethylsilyl)methyl isocyanide. Tetrazylmethyl-removal is compatible with use on biomacromolecules, in cellular environments, and in living organisms as demonstrated by cytotoxicity experiments and fluorophore-release studies on proteins and in zebrafish embryos. By combining tetrazylmethyl derivatives with previously reported tetrazine-responsive 3-isocyanopropyl groups, it was possible to liberate two fluorophores in vertebrates from a single bioorthogonal reaction. This chemistry will open new opportunities towards applications involving multiplexed release schemes and is a valuable asset to the growing toolbox of bioorthogonal dissociative reactions

A method for the ultrastructural preservation of tiny percutaneous needle biopsy material from skeletal muscle

Skeletal muscle biopsies require transecting the muscle fibers resulting, in structural damage near the cut ends. Classically, the optimal ultrastructural preservation has been obtained by the use of relatively large biopsies in which the tissue fibers are restrained by ligating to a suitable retaining support prior to excision, and by examining regions at some distance from the cut ends. However, these methods require invasive surgical procedures. In the present study, we present and substantiate an alternative approach that allows for the excellent ultrastructural preservation of needle biopsy samples, even the very small samples obtained through tiny percutaneous needle biopsy (TPNB). TPNB represents an advantage, relative to standard muscle biopsy techniques and to other needle biopsies currently in use, as in addition to not requiring a skin incision, it leaves no scars in the muscle and requires an extremely brief recovery period. It is most appropriate for obtaining repeated samples in horizontal studies, e.g., in order to follow changes with athletic training and/or aging in a single individual and for studies of sarcopenic muscles in elderly patients. Due to the small size of the sample, TPNB may present limited usefulness for classical pathology diagnostics. However, it offers the major advantage of allowing multiple samples within a single session and this may be useful under specific circumstances

Phosphorus efficiency in brazilian soybean cultivars.

The primary goal of this study was to evaluate the phosphorus uptake and use efficiency in Brazilian soybean cultivars, besides root morphology and architecture characteristics related to phosphorus uptake, carrying out two greenhouse experiments. The experiment 1 was completely randomised, with 56 treatments (soybean cultivars) and 3 replicates. Experiment 2 was completely randomised design with three replicates, and the ten cultivars (greater and lower efficiency) were selected for this assay according to their ability to absorb phosphorus less available determined in experiment 1. The study was carried out at Center of Nuclear Energy in Agriculture, University of São Paulo, Brazil, between February 2011 and August 2012. The isotopic dilution technique was used in the first experiment to assess the phosphorus availability in the soil and to determine the ability of plants to access labile phosphorus by measuring the specific activity of plants grown in soil labeled with radioactive phosphorus. Nine cultivars showed greater phosphorus uptake and used efficiency. The second experiment evaluated the root morphology and architecture. The cultivars with greater uptake efficiency have root morphology and architecture characteristics that favour acquisition of phosphorus from soil compartments that are inaccessible to other cultivars. Phosphorus uptake by plants was not affected by soybean seeds phosphorus content. Identification of these cultivars is very important because it could enable soybean farming in low fertility soils, reducing fertiliser dependence

Leptonic Flavor and CP Violation

We discuss how neutrino oscillation experiments can probe new sources of leptonic flavor and CP violation.Comment: 8 pages, latex, no figures. Invited talk given at KAON 2001, Pisa, Italy, June 12 - 17, 200

Measurement of the K_L \to \pi\mu\nu form factor parameters with the KLOE detector

Using 328 pb^{-1}of data collected at DAFNE corresponding to $\sim$ 1.8 million $K_L\to \pi\mu\nu$ decays, we have measured the $K_{\mu 3}$ form factor parameters. The structure of the $K-\pi$ vector-current provides information about the dynamics of the strong interaction; its knowledge is necessary for evaluation of the phase-space integral required for measuring the CKM matrix element $V_{us}$ and for testing lepton universality in kaon decays. Using a new parametrization for the vector and scalar form factors, we find $\lambda_+$=\pt(25.7\pm 0.6),-3, and $\lambda_0$=\pt(14.0\pm 2.1),-3,. Our result for $\lambda_0$, together with recent lattice calculations of $f_\pi$, $f_K$ and $f(0)$, satisfies the Callan-Trieman relatio

A global fit to determine the pseudoscalar mixing angle and the gluonium content of the eta' meson

We update the values of the eta-eta' mixing angle and of the eta' gluonium content by fitting our measurement R_phi = BR(phi to eta' gamma)/ BR(phi to eta gamma) together with several vector meson radiative decays to pseudoscalars (V to P gamma), pseudoscalar mesons radiative decays to vectors (P to V gamma) and the eta' to gamma gamma, pi^0 to gamma gamma widths. From the fit we extract a gluonium fraction of Z^2_G = 0.12 +- 0.04, the pseudoscalar mixing angle psi_P = (40.4 +- 0.6) degree and the phi-omega mixing angle psi_V = (3.32 +- 0.09) degree. Z^2_G and psi_P are fairly consistent with those previously published. We also evaluate the impact on the eta' gluonium content determination of future experimental improvements of the eta' branching ratios and decay width.Comment: 13 pages, 7 figures to submit to JHE