Анализ конструкций камер приема и пуска средств очистки и диагностики трубопровода

В выпускной квалификационной работе проведен анализ различных конструкций камер запуска-приема очистных устройств и диагностических снарядов для магистрального нефтепровода. Рассчитана необходимая толщина стенки элементов камеры.In the final qualification work, an analysis was made of various pig trap station was considered using for the main oil pipeline. The required thickness of the wall of the chamber elements was calculated

Increased peri-ductal collagen micro-organization may contribute to raised mammographic density

BACKGROUND: High mammographic density is a therapeutically modifiable risk factor for breast cancer. Although mammographic density is correlated with the relative abundance of collagen-rich fibroglandular tissue, the causative mechanisms, associated structural remodelling and mechanical consequences remain poorly defined. In this study we have developed a new collaborative bedside-to-bench workflow to determine the relationship between mammographic density, collagen abundance and alignment, tissue stiffness and the expression of extracellular matrix organising proteins. METHODS: Mammographic density was assessed in 22 post-menopausal women (aged 54–66 y). A radiologist and a pathologist identified and excised regions of elevated non-cancerous X-ray density prior to laboratory characterization. Collagen abundance was determined by both Masson’s trichrome and Picrosirius red staining (which enhances collagen birefringence when viewed under polarised light). The structural specificity of these collagen visualisation methods was determined by comparing the relative birefringence and ultrastructure (visualised by atomic force microscopy) of unaligned collagen I fibrils in reconstituted gels with the highly aligned collagen fibrils in rat tail tendon. Localised collagen fibril organisation and stiffness was also evaluated in tissue sections by atomic force microscopy/spectroscopy and the abundance of key extracellular proteins was assessed using mass spectrometry. RESULTS: Mammographic density was positively correlated with the abundance of aligned periductal fibrils rather than with the abundance of amorphous collagen. Compared with matched tissue resected from the breasts of low mammographic density patients, the highly birefringent tissue in mammographically dense breasts was both significantly stiffer and characterised by large (>80 μm long) fibrillar collagen bundles. Subsequent proteomic analyses not only confirmed the absence of collagen fibrosis in high mammographic density tissue, but additionally identified the up-regulation of periostin and collagen XVI (regulators of collagen fibril structure and architecture) as potential mediators of localised mechanical stiffness. CONCLUSIONS: These preliminary data suggest that remodelling, and hence stiffening, of the existing stromal collagen microarchitecture promotes high mammographic density within the breast. In turn, this aberrant mechanical environment may trigger neoplasia-associated mechanotransduction pathways within the epithelial cell population. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13058-015-0664-2) contains supplementary material, which is available to authorized users

Controls on tidal sedimentation and preservation : Insights from numerical tidal modelling in the Late Oligocene–Miocene South China Sea, Southeast Asia

Numerical tidal modelling, when integrated with other geological datasets, can significantly inform the analysis of physical sedimentation processes and the depositional and preservational record of ancient tide-influenced shoreline–shelf systems. This is illustrated in the Oligo–Miocene of the South China Sea (SCS), which experienced significant changes in basin physiography and where tide-influenced, shoreline–shelf deposition is preserved in ca 10 sub-basins. Palaeogeographic reconstructions, palaeotidal modelling and regional sedimentary facies analysis have been integrated in order to evaluate the spatial–temporal evolution and physiographic controls on tidal sedimentation and preservation during the ca 25 Myr Oligo–Miocene record in the SCS. Palaeotidal modelling, using an astronomically forced and global tidal model (Fluidity) at a maximum 10 km resolution, indicates that spring tides along Late Oligocene–Middle Miocene coastlines were predominantly mesotidal– macrotidal and capable of transporting sand, which reflects two main conditions: (1) increased tidal inflow through wider ocean connections to the Pacific Ocean; and (2) tidal amplification resulting from constriction of the tidal wave in a ‘blind gulf’ type of basin morphology. Since the Middle–Late Miocene, a reduction in the amplitude and strength of tides in the SCS was mainly due to diminishing tidal inflow from the Pacific Ocean caused by the northward movement of the Philippines and Izu-Bonin-Mariana arc. Sensitivity tests to palaeogeographic and palaeobathymetric uncertainty indicate that regional–scale (100–1000s 29 km) palaeogeographic changes influencing tidal inflow versus outflow can override local30 scale (1–100s km) changes to tidal resonance and convergence effects (funnelling and shoaling), such as shelf width and shoreline geometry. Palaeotidal model results compare favourably to the distribution and sedimentary fabric of Oligo–Miocene, tide-influenced, shoreline–shelf successions in peripheral SCS basins. However, the preservation potential of tidal deposits is lower in open coastline environments, probably due to enhanced reworking during storms and river floods

Large-scale genome-wide analysis identifies genetic variants associated with cardiac structure and function

BACKGROUND: Understanding the genetic architecture of cardiac structure and function may help to prevent and treat heart disease. This investigation sought to identify common genetic variations associated with inter-individual variability in cardiac structure and function. METHODS: A GWAS meta-analysis of echocardiographic traits was performed, including 46,533 individuals from 30 studies (EchoGen consortium). The analysis included 16 traits of left ventricular (LV) structure, and systolic and diastolic function. RESULTS: The discovery analysis included 21 cohorts for structural and systolic function traits (n = 32,212) and 17 cohorts for diastolic function traits (n = 21,852). Replication was performed in 5 cohorts (n = 14,321) and 6 cohorts (n = 16,308), respectively. Besides 5 previously reported loci, the combined meta-analysis identified 10 additional genome-wide significant SNPs: rs12541595 near MTSS1 and rs10774625 in ATXN2 for LV end-diastolic internal dimension; rs806322 near KCNRG, rs4765663 in CACNA1C, rs6702619 near PALMD, rs7127129 in TMEM16A, rs11207426 near FGGY, rs17608766 in GOSR2, and rs17696696 in CFDP1 for aortic root diameter; and rs12440869 in IQCH for Doppler transmitral A-wave peak velocity. Findings were in part validated in other cohorts and in GWAS of related disease traits. The genetic loci showed associations with putative signaling pathways, and with gene expression in whole blood, monocytes, and myocardial tissue. CONCLUSION: The additional genetic loci identified in this large meta-analysis of cardiac structure and function provide insights into the underlying genetic architecture of cardiac structure and warrant follow-up in future functional studies. FUNDING: For detailed information per study, see Acknowledgments.This work was supported by a grant from the US National Heart, Lung, and Blood Institute (N01-HL-25195; R01HL 093328 to RSV), a MAIFOR grant from the University Medical Center Mainz, Germany (to PSW), the Center for Translational Vascular Biology (CTVB) of the Johannes Gutenberg-University of Mainz, and the Federal Ministry of Research and Education, Germany (BMBF 01EO1003 to PSW). This work was also supported by the research project Greifswald Approach to Individualized Medicine (GANI_MED). GANI_MED was funded by the Federal Ministry of Education and Research and the Ministry of Cultural Affairs of the Federal State of Mecklenburg, West Pomerania (contract 03IS2061A). We thank all study participants, and the colleagues and coworkers from all cohorts and sites who were involved in the generation of data or in the analysis. We especially thank Andrew Johnson (FHS) for generation of the gene annotation database used for analysis. We thank the German Center for Cardiovascular Research (DZHK e.V.) for supporting the analysis and publication of this project. RSV is a member of the Scientific Advisory Board of the DZHK. Data on CAD and MI were contributed by CARDIoGRAMplusC4D investigators. See Supplemental Acknowledgments for consortium details. PSW, JFF, AS, AT, TZ, RSV, and MD had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis