1,652 research outputs found
Ovarian steroids regulate tachykinin and tachykinin receptor gene expression in the mouse uterus
<p>Abstract</p> <p>Background</p> <p>In the mouse uterus, pregnancy is accompanied by changes in tachykinin and tachykinin receptor gene expression and in the uterotonic effects of endogenous tachykinins. In this study we have investigated whether changes in tachykinin expression and responses are a result of changes in ovarian steroid levels.</p> <p>Methods</p> <p>We quantified the mRNAs of tachykinins and tachykinin receptors in uteri from ovariectomized mice and studied their regulation in response to estrogen and progesterone using real-time quantitative RT-PCR. Early (3 h) and late (24 h) responses to estrogen were evaluated and the participation of the estrogen receptors (ER), ERalpha and ERbeta, was analyzed by treating mice with propylpyrazole triol, a selective ERalpha agonist, or diarylpropionitrile, a selective agonist of ERbeta.</p> <p>Results</p> <p>All genes encoding tachykinins (Tac1, Tac2 and Tac4) and tachykinin receptors (Tacr1, Tacr2 and Tacr3) were expressed in uteri from ovariectomized mice. Estrogen increased Tac1 and Tacr1 mRNA after 3 h and decreased Tac1 and Tac4 expression after 24 h. Tac2 and Tacr3 mRNA levels were decreased by estrogen at both 3 and 24 h. Most effects of estrogen were also observed in animals treated with propylpyrazole triol. Progesterone treatment increased the levels of Tac2.</p> <p>Conclusion</p> <p>These results show that the expression of tachykinins and their receptors in the mouse uterus is tightly and differentially regulated by ovarian steroids. Estrogen effects are mainly mediated by ERalpha supporting an essential role for this estrogen receptor in the regulation of the tachykinergic system in the mouse uterus.</p
Understanding pseudo-albinism in sole (Solea senegalensis): a transcriptomics and metagenomics approach
Pseudo-albinism is a pigmentation disorder observed in flatfish aquaculture with a complex, multi-factor aetiology. We tested the hypothesis that pigmentation abnormalities are an overt signal of more generalised modifications in tissue structure and function, using as a model the Senegalese sole and two important innate immune barriers, the skin and intestine, and their microbiomes. Stereological analyses in pseudo-albino sole revealed a significantly increased mucous cell number in skin (P < 0.001) and a significantly thicker muscle layer and lamina propria in gut (P < 0.001). RNA-seq transcriptome analysis of the skin and gut identified 573 differentially expressed transcripts (DETs, FDR < 0.05) between pseudo-albino and pigmented soles (one pool/tissue from 4 individuals/phenotype). DETs were mainly linked to pigment production, skin structure and regeneration and smooth muscle contraction. The microbiome (16 S rRNA analysis) was highly diverse in pigmented and pseudo-albino skin but in gut had low complexity and diverged between the two pigmentation phenotypes. Quantitative PCR revealed significantly lower loads of Mycoplasma (P < 0.05) and Vibrio bacteria (P < 0.01) in pseudo-albino compared to the control. The study revealed that pseudo-albinism in addition to pigmentation changes was associated with generalised changes in the skin and gut structure and a modification in the gut microbiome.Agência financiadora
H2020 European Funds MSCA-RISE project
691102
Portuguese national funds from FCT - Foundation for Science and Technology
UID/Multi/04326/2019
Portuguese national funds from the operational programme CRESC Algarve 2020
EMBRC. PT ALG-01-0145-FEDER-022121
Portuguese national funds from the operational programme COMPETE 2020
EMBRC. PT ALG-01-0145-FEDER-022121
European Union (EU)
654008
Fundacao para a Ciencia e a Tecnologia (FCT)
SFRH/BPD/84033/2012
Portuguese Institute for Employment and Vocational Training
0068/ET/18info:eu-repo/semantics/publishedVersio
Development of a metric of aquatic invertebrates for volunteers (MAIV): a simple and friendly biotic metric to assess ecological quality of streams
Citizen science activities, involving local people in volunteer-supported and sustainable
monitoring programs, are common. In this context, the objective of the present work was to develop a
simple Metric of Aquatic Invertebrates for Volunteers (MAIV), including a user-friendly tool that can
be easily accessed by volunteers, and to evaluate the e ciency of a volunteer monitoring program
following an audit procedure. To obtain MAIV values, macroinvertebrate communities were reduced
to 18 surrogate taxa, which represented an acceptable compromise between simplicity, e ciency, and
reproducibility of the data, compared to the regular Water Framework Directive monitoring. When
compared to results obtained with the National Classification System of Portugal, MAIV accurately
detected moderate, poor, and bad ecological status. Thus, MAIV can be used by volunteers as a
complement to the o cial monitoring program, as well as a prospective early warning tool for
local problems related to ecological quality. Volunteers were students supervised by their teachers.
Results obtained by volunteers were compared to results obtained by experts on macroinvertebrate
identification to measure the e ciency of the procedure, by counting gains and losses on sorting,
and identification. Characteristics of groups of volunteers (age and school level) did not influence
significantly the e ciency of the procedure, and generally results of volunteers and experts matched.info:eu-repo/semantics/publishedVersio
Project-led Engineering Education : assessment model and rounding errors analysis
This paper presents an analysis of the assessment model used in an interdisciplinary Project-Led
Education (PLE) process implemented in the Integrated Master Course on Industrial Management
and Engineering (IME) at University of Minho. PLE is an innovative educational methodology
which makes use of active learning, promoting higher levels of motivation and students’
autonomy. The assessment model is based on multiple evaluation components with different
weights. Each component can be evaluated by several teachers involved in different Project
Supporting Courses (PSC). This model can be affected by different types of errors, namely: (1)
rounding errors, and (2) non-uniform criteria of rounding the grades. A rigorous analysis of the
assessment model was made and the rounding errors involved on each project component
characterised. This resulted in a global maximum error of 0.308 on the individual student project
grade, in a 0 to 100 scale. This analysis intended to improve not only the reliability of the
assessment results, but also teachers’ awareness to this problem. Recommendations are also made
in order to improve the assessment model and reduce the rounding errors as much as possible
Measurement rounding errors in an assessment model of project led Engineering Education
This paper analyzes the rounding errors that
occur in the assessment of an interdisciplinary Project-Led
Education (PLE) process implemented in the Integrated
Master degree on Industrial Management and Engineering
(IME) at University of Minho. PLE is an innovative educational
methodology which makes use of active learning,
promoting higher levels of motivation and students’ autonomy.
The assessment model is based on multiple evaluation
components with different weights. Each component can be
evaluated by several teachers involved in different Project
Supporting Courses (PSC). This model can be affected by
different types of errors, namely: (1) rounding errors, and
(2) non-uniform criteria of rounding the grades. A rigorous
analysis of the assessment model was made and the rounding
errors involved on each project component were characterized
and measured. This resulted in a global maximum
error of 0.308 on the individual student project grade, in a 0
to 100 scale. This analysis intended to improve not only the
reliability of the assessment results, but also teachers’
awareness of this problem. Recommendations are also made
in order to improve the assessment model and reduce the
rounding errors as much as possible
Shear strength of adhesively bonded polyolefins with minimal surface preparation
Interest in polyethylene and polypropylene bonding has increased in the last years. However, adhesive joints with adherends which are of low surface energy and which are chemically inert present several difficulties. Generally, their high degree of chemical resistance to solvents and dissimilar solubility parameters limit the usefulness of solvent bonding as a viable assembly technique. One successful approach to adhesive bonding of these materials involves proper selection of surface pre-treatment prior to bonding. With the correct pre-treatment it is possible to glue these materials with one or more of several adhesives required by the applications involved. A second approach is the use of adhesives without surface pre-treatment, such as hot melts, high tack pressure-sensitive adhesives, solvent-based specialty adhesives and, more recently, structural acrylic adhesives as such 3M DP-8005® and Loctite 3030®.
In this paper, the shear strengths of two acrylic adhesives were evaluated using the lap shear test method ASTM D3163 and the block shear test method ASTM D4501. Two different industrial polyolefins (polyethylene and polypropylene) were used for adherends. However, the focus of this study was to measure the shear strength of polyethylene joints with acrylic adhesives. The effect of abrasion was also studied. Some test specimens were manually abraded using 180 and 320 grade abrasive paper. An additional goal of this work was to examine the effect of temperature and moisture on mechanical strength of adhesive joints
Software methodologies for the engineering of service-oriented industrial automation: the continuum project
Indexado InspecService-orientation represents a new wave of features and solutions by bringing closer Information Technology to the industrial domain, particularly factory shop floors. The service-oriented automation software entities (designated here by bots) used in such approach requires a short set of methodologies and software targeting their specification for both computer systems and embedded automation devices. The present work explains the adopted methodologies and software developments for the engineering of service-based automation systems. The main contents focus on the specification of a framework for the development of bots and supporting engineering tools that are part of the Continuum project. The paper also does an overview over the engineering steps from the system design to the operation, and focuses the importance of the maintenance of automation bots. Such applications will contribute to decrease the development time and reduce the components' interdependency, offering enough flexibility for automatic reconfiguration of shop-floor layouts.The authors would like to thank the European Commission and the partners of the EU IST FP6 project “Service-Oriented Cross-layer infrastructure for Distributed smart Embedded devices” (SOCRADES), the EU FP6 “Network of Excellence for Innovative Production Machines and Systems” (I*PROMS), and the European ICT FP7 project “Cooperating Objects Network of Excellence” (CONET) for their support
Strategic environmental assessment in TrĂłia (Portugal)
Proceedings of the First International Conference on Coastal Conservation and Management in the Atlantic and Mediterranean, p. 91-98Over the last two decades, the TrĂłia peninsula (SW coast of Portugal) has been designated by the Portuguese authorities both as a protected area and as an area of tourism development. The private investor that took over the tourism developments in TrĂłia asked the Institute of Marine Research (IMAR) to undertake an SEA, to help frame
the design and management of the future resort. SEA results were changed by local/central government decisions,
granting “more” than stakeholder/owner’s proposal, in terms of loads and densities. This process, as well as the main
characteristics and results of the SEA, are presented and discussed
Libro de Caualleria de Jineta con la doctrina de los mehores autores [Manuscrito] :]dedicado Alexmo.S.D. francisco fernandez de Cordoua
Contiene: Libro de Caualleria de Jineta con la doctrina de los mehores autores : h. IV-VI, VIII-XIII, 123 h. -- Arte y destresa de torear : h. 124-135, h. [136-173]Enc. en perg. con restos de correĂllasEn la port.: "Con su tabla de los Capitulos al principio del libro"PerteneciĂł a la biblioteca de Sebastián Soto CortĂ©s y, posteriormente, a la de Roque PidalAEn la contracub. ant.: "TraducciĂłn del primo del autor D. Juan Suárez de Somoza y Torres hecha en Madrid en 1678. Ăšnica conocida. 3.000 pts.
Production, immobilization and synthesis of pharmacological derivatives of lipase B from Candida antarctica in Pichia pastoris
Lipase B from Candida antarctica (CALB) is widely used because of its excellent enantioselectivity. Producing this recombinant lipase in Pichia pastoris has advantages since it can be cultured in simple media and can reach high cell densities. This capability is especially important when using a constitutive promoter for lipase production, as here. The PPGK promoter is similar to the well-known PGAP promoter and also circumvents the need for inducing production with methanol, which is a hazard when used on a large scale and would increase the downstream production costs, which could be prohibitive for pharmaceutical products.
This study tested two main fermentation strategies: continuous and fed-batch. In both cultures, different specific growth rates occurred (0.05, 0.10, 0.15 and 0.18h–1), and process parameters (qP, qS, YX/S, YP/X, YP/S) were evaluated in order to properly compare them. The highest specific production rate achieved with a continuous culture was 57.71 U/gX.h with µ=0.15 h–1 and 16 U/gX.h with µ=0.14 h–1 for a fed-batch culture. Productivity decreased dramatically near the µmax (0.18 h–1) for P. pastoris (57.6% lower). The best strategy for production was calculated over a three-month period. In both cases, the enzyme is secreted to the supernatant and purification is needed to ensure that only LIPB participates in further reactions. The immobilization process is ideal because purification and concentration is achieved in only one step, reusability is made possible, and in certain cases, stability and efficiency are boosted. Hydrophobic core-shell polymeric supports synthesized by a combined suspension and emulsion polymerization process have shown good potential for lipase immobilization procedures and were used in this study, compared to traditional supports such as Accurel, in order to determine their efficiency.
After the enzyme was immobilized, the reactions included the resolution of (±)-1,3,5-O-benzyl-myo-inositol (DL-1) via acylation using vinyl acetate in hexane, and resolution of (±)-1,2-O- isopropylidene-3,6-di-O-benzyl-myo-inositol (DL-2) via acylation using vinyl acetate (solvent-free system). The support used directly affected the reaction, but trends were observed. In general, the recombinant lipase produced (LIPB) had higher resolutions than the commercial lipase (CALB, Novozym 435). In the resolution of DL-1 and DL-2 via transesterification (using different media), LIPB immobilized in Accurel or PS-co-DVB/PS-co-DVB showed more activity per enzyme molecule than CALB immobilized in similar supports, while when immobilized in PMMA-co-DVB/ PMMA-co-DVB the activities of the two enzymes were similar. The recombinant LIPB immobilized on PS-co-DVB proved to be the most efficient in the enantioselective resolution of both racemic derivatives, DL-1 and DL-2. The productivity for DL-2 resolution was 50% higher than the commercial Novozym 435, and the new derivative was operationally more stable than Novozym 435. The products obtained had a high level of purity (ee of 99% for both derivatives). Both products of the enantio-selective reaction, L-2 and L-5, obtained from the racemic derivatives (DL-1 and DL-2, respectively), are intermediates from different pharmacological pathways involved in the synthesis of building blocks for drugs that inhibit the etiological agent of Chagas disease, Trypanosoma cruzi
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