An End to a Means: How DNA-End Structure Shapes the Double-Strand Break Repair Process

Endogenously-arising DNA double-strand breaks (DSBs) rarely harbor canonical 5'-phosphate, 3'-hydroxyl moieties at the ends, which are, regardless of the pathway used, ultimately required for their repair. Cells are therefore endowed with a wide variety of enzymes that can deal with these chemical and structural variations and guarantee the formation of ligatable termini. An important distinction is whether the ends are directly "unblocked" by specific enzymatic activities without affecting the integrity of the DNA molecule and its sequence, or whether they are "processed" by unspecific nucleases that remove nucleotides from the termini. DNA end structure and configuration, therefore, shape the repair process, its requirements, and, importantly, its final outcome. Thus, the molecular mechanisms that coordinate and integrate the cellular response to blocked DSBs, although still largely unexplored, can be particularly relevant for maintaining genome integrity and avoiding malignant transformation and cancer.España, Junta de Andalucía SAF2017-89619-R, CVI-7948European Research Council (ERC-CoG-2014-647359

Informative discourse and migration. Analysis of television­production routines and sociocultural diversity in Andalusia

Las limitaciones y rutinas de la producción periodística son aspectos que, con frecuencia, quedan rele­gados en el abordaje del tratamiento de la diversidad en los medios pero que tienen un gran peso en la construcción de los imaginarios y en los discursos multimodales sobre la migración. A partir del análi­sis de las noticias de Canal Sur Televisión y de entrevistas a los profesionales de este medio, se estudia cómo se construye mediáticamente la diversidad en esta cadena pública autonómica, atendiendo a los lí­mites impuestos por: los criterios periodísticos, la toma de decisiones profesionales, la influencia de los discursos sociales y políticos, así como por el cumplimiento de las recomendaciones del libro de estilo.Como herramientas metodológicas, se combinan el análisis de contenido y la entrevista en profundidad, un total de dieciséis, realizada a periodistas y a operadores de cámara de Málaga, Cádiz, Almería, Alge­ciras y Granada.imitations and daily routine of press activity are aspects that are usually put away when we speak about the approach to the diversity within the media, but, on the other hand, they have great importance when it comes to build an imaginary and in the multimodal discourses about migration. Using the analysis ofCanal Sur Televisión ́s news together with the interviews to professional workers of the media, it ́s been studied the way to build, speaking about the media world, the diversity in this regional public channel, attending to the boundaries established by: press criteria, making professional decisions, influence of so­cial and politics discourses and the observance of the Style Guide. As methodological tools, analysis of contents and in­ depth interviews to journalists and cameramen from Málaga, Cádiz, Almería, Algecirasy Granada are combined, a total of sixteen.735-75

Hand hygiene technique quality evaluation in nursing and medicine students of two academic courses

Objetivo:assim como as mãos dos professionais de saúde, as mãos dos estudantes de Enfermagem e Medicina durante os estágios clínicos podem funcionar como veículo de transmissão das infecções hospitalares.Método:estudo descritivo com estudantes de Graduação em Enfermagem e Medicina. Avaliou-se a qualidade da técnica de higiene das mãos por meio de prova visual a partir de solução hidro-alcoólica marcada com fluoresceína e lâmpada de luz ultravioleta.Resultados:avaliaram-se 546 alunos, 73,8% da Graduação em Medicina e 26,2% da Enfermagem. As áreas das mãos com correta distribuição de sabão foram as palmas (92,9%); as áreas não esfregadas corretamente foram os polegares (55,1%). Em ambas as mãos, a higienização foi muito boa em 24,7%, boa em 29,8%, regular em 25,1% e má em 20,3%. Os piores foram: os homens, os estudantes de Enfermagem e os estudantes do primeiro ano de escola. Não houve diferenças significativas por idade.Conclusões:a técnica de higiene de mãos não foi realizada de modo eficaz. O papel educativo é fundamental para sedimentar as bases de boas práticas na higienização das mãos, em conhecimentos teóricos e no desenvolvimento de habilidades e reforço de boas práticasObjective: because they are health professionals, nursing and medical students' hands during internships can function as a transmission vehicle for hospital-acquired infections.Method: a descriptive study with nursing and medical degree students on the quality of the hand hygiene technique, which was assessed via a visual test using a hydroalcoholic solution marked with fluorescence and an ultraviolet lamp.Results: 546 students were assessed, 73.8% from medicine and 26.2% from nursing. The area of the hand with a proper antiseptic distribution was the palm (92.9%); areas not properly scrubbed were the thumbs (55.1%). 24.7% was very good in both hands, 29.8% was good, 25.1% was fair, and 20.3% was poor. The worst assessed were the male, nursing and first year students. There were no significant differences in the age groups.Conclusions: hand hygiene technique is not applied efficiently. Education plays a key role in setting a good practice base in hand hygiene, theoretical knowledge, and in skill development, as well as good practice reinforcement.Objetivo:al igual que los profesionales sanitarios, las manos de los estudiantes de enfermería y medicina durante las estancias clínicas pueden funcionar como vehículo de trasmisión de las infecciones nosocomiales.Método:estudio descriptivo con estudiantes de Grado de Enfermería y de Medicina. Se evaluó la calidad de la técnica de la higiene de manos por prueba visual con solución hidroalcohólica marcada con fluoresceína y lámpara de luz ultravioleta.Resultados:se evaluó a 546 alumnos, Grado de Medicina 73,8% y 26,2% de Enfermería. Las zonas de las manos con una correcta distribución de desinfectante fueron las palmas (92,9%); las no frotadas correctamente fueron los pulgares (55,1%). En ambas manos fue muy buena en el 24,7%, buena en el 29,8%, regular en 25,1% y mala en 20,3%. Los peores han sido: los hombres, los estudiantes de enfermería y los de primer año de estudio. No hubo diferencias significativas por edad.Conclusiones:no se realizó la técnica de higiene de manos de una manera eficaz. El papel de la educación es fundamental para sentar las bases de buenas prácticas en higiene de manos, en conocimientos teóricos y en el desarrollo de habilidades y el refuerzo de buenas práctica

Evaluación de la calidad de la técnica de higiene de manos en alumnos de enfermería y medicina en dos cursos académicos

Objetivo: al igual que los profesionales sanitarios, las manos de los estudiantes de enfermería y medicina durante las estancias clínicas pueden funcionar como vehículo de trasmisión de las infecciones nosocomiales. Método: estudio descriptivo con estudiantes de Grado de Enfermería y de Medicina. Se evaluó la calidad de la técnica de la higiene de manos por prueba visual con solución hidroalcohólica marcada con fluoresceína y lámpara de luz ultravioleta. Resultados: se evaluó a 546 alumnos, Grado de Medicina 73,8% y 26,2% de Enfermería. Las zonas de las manos con una correcta distribución de desinfectante fueron las palmas (92,9%); las no frotadas correctamente fueron los pulgares (55,1%). En ambas manos fue muy buena en el 24,7%, buena en el 29,8%, regular en 25,1% y mala en 20,3%. Los peores han sido: los hombres, los estudiantes de enfermería y los de primer año de estudio. No hubo diferencias significativas por edad. Conclusiones: no se realizó la técnica de higiene de manos de una manera eficaz. El papel de la educación es fundamental para sentar las bases de buenas prácticas en higiene de manos, en conocimientos teóricos y en el desarrollo de habilidades y el refuerzo de buenas prácticasObjective: because they are health professionals, nursing and medical students’ hands during internships can function as a transmission vehicle for hospital-acquired infections. Method: a descriptive study with nursing and medical degree students on the quality of the hand hygiene technique, which was assessed via a visual test using a hydroalcoholic solution marked with fluorescence and an ultraviolet lamp. Results: 546 students were assessed, 73.8% from medicine and 26.2% from nursing. The area of the hand with a proper antiseptic distribution was the palm (92.9%); areas not properly scrubbed were the thumbs (55.1%). 24.7% was very good in both hands, 29.8% was good, 25.1% was fair, and 20.3% was poor. The worst assessed were the male, nursing and first year students. There were no significant differences in the age groups. Conclusions: hand hygiene technique is not applied efficiently. Education plays a key role in setting a good practice base in hand hygiene, theoretical knowledge, and in skill development, as well as good practice reinforcement.Objetivo: assim como as mãos dos professionais de saúde, as mãos dos estudantes de Enfermagem e Medicina durante os estágios clínicos podem funcionar como veículo de transmissão das infecções hospitalares. Método: estudo descritivo com estudantes de Graduação em Enfermagem e Medicina. Avaliou-se a qualidade da técnica de higiene das mãos por meio de prova visual a partir de solução hidro-alcoólica marcada com fluoresceína e lâmpada de luz ultravioleta. Resultados: avaliaram-se 546 alunos, 73,8% da Graduação em Medicina e 26,2% da Enfermagem. As áreas das mãos com correta distribuição de sabão foram as palmas (92,9%); as áreas não esfregadas corretamente foram os polegares (55,1%). Em ambas as mãos, a higienização foi muito boa em 24,7%, boa em 29,8%, regular em 25,1% e má em 20,3%. Os piores foram: os homens, os estudantes de Enfermagem e os estudantes do primeiro ano de escola. Não houve diferenças significativas por idade. Conclusões: a técnica de higiene de mãos não foi realizada de modo eficaz. O papel educativo é fundamental para sedimentar as bases de boas práticas na higienização das mãos, em conhecimentos teóricos e no desenvolvimento de habilidades e reforço de boas práticaspeerReviewe

Scattering of 11 Be around the Coulomb barrier

Preliminary results on the 11Be+120Sn quasielastic scattering as well as the 11Be → 10Be + n breakup channel are presented in this work. The angular distributions of these channels were measured at REX-ISOLDE-CERN. The accuracy and angular range of the presented results provide stronger constrains to the theoretical interpretation than existing published results. We compare these new data with coupled-channel (CC) and continuum-discretized coupled-channel (CDCC) calculations. The role played by transfer and breakup channels in the elastic scattering is discussed.CICYT FPA2007- 62170MEC FINURA (FPA2007-63074) CPAN (CSD 2007-00042)DGICYT FPA2006-13807-c02-01 CSD-2007-0004

Phenotypic and environmental correlates of natal dispersal in a long-lived territorial vulture

Natal dispersal, the movement between the birth and the first breeding site, has been rarely studied in long-lived territorial birds with a long-lasting pre-breeding stage. Here we benefited from the long-term monitoring programs of six populations of Egyptian vultures (Neophron percnopterus) from Spain and France to study how the rearing environment determines dispersal. For 124 vultures, we recorded a median dispersal distance of 48km (range 0-656km). Linear models were used to assess the effect of population and individual traits on dispersal distance at two spatial scales. Dispersal distances were inversely related to vulture density in the natal population, suggesting that birds perceive the abundance of conspecifics as a signal of habitat quality. This was particularly true for declining populations, so increasing levels of opportunistic philopatry seemed to arise in high density contexts as a consequence of vacancies created by human-induced adult mortality. Females dispersed further than males, but males were more sensitive to the social environment, indicating different dispersal tactics. Both sexes were affected by different individual attributes simultaneously and interactively with this social context. These results highlight that complex phenotype-by-environment interactions should be considered for advancing our understanding of dispersal dynamics in long-lived organisms.Funds were partially provided by Diputación General de Aragón, Junta de Andalucía, Comunidad de Bardenas and Projects CGL2009-12753-C02-02, CGL2009-12753-C02-01/BOS and CGL2010-15726 of Spanish Ministerio of Economía y Competitividad. ACA was supported by a contract Juan de la Cierva Incorporación (IJCI-2014-20744; Ministerio de Economía y Competitividad, Spain) and a Post-Doc contract Programa Viçent Mut of Govern Balear, Spain (PD/039/2017). EA was supported by La Caixa-Severo Ochoa International PhD Program 2015, JM was supported by a Basque Government predoctoral fellowship PRE_2018_2_0112 (2017)

Alternative Eco-Friendly Methods in the Control of Post-Harvest Decay of Tropical and Subtropical Fruits

The effectiveness on several fruits by the application of alternative methods against fungi is summarized in the present chapter. Several investigations have reported the efficacy of these technologies for controlling fungal infections. Currently, high post-harvest loses have been reported due to several factors such as inefficient management, lack of training for farmers, and problems with appropriate conditions for storage of fruits and vegetables. Even now, in many countries, post-harvest disease control is led by the application of chemical fungicides. However, in this time, awareness about fungi resistance, environmental, and health issues has led to the research of eco-friendly and effective alternatives for disease management. The pathogen establishment on fruits can be affected by the application of GRAS compounds like chitosan, essential oils, salts, among others; besides, their efficacy can be enhanced by their combination with other technologies like ultrasound. Thus, the applications of these alternatives are suitable approaches for post-harvest management of fruits

Poly[ADP-Ribose] Polymerase-1 Expression Is Related To Cold Ischemia, Acute Tubular Necrosis, and Delayed Renal Function In Kidney Transplantation

Cold ischemia time especially impacts on outcomes of expanded-criteria donor (ECD) transplantation. Ischemia-reperfusion (IR) injury produces excessive poly[ADP-Ribose] Polymerase-1 (PARP-1) activation. The present study explored the hypothesis that increased tubular expression of PARP-1 contributes to delayed renal function in suboptimal ECD kidney allografts and in non-ECD allografts that develop posttransplant acute tubular necrosis (ATN)

DVINO: A RISC-V vector processor implemented in 65nm technology

This paper describes the design, verification, implementation and fabrication of the Drac Vector IN-Order (DVINO) processor, a RISC-V vector processor capable of booting Linux jointly developed by BSC, CIC-IPN, IMB-CNM (CSIC), and UPC. The DVINO processor includes an internally developed two-lane vector processor unit as well as a Phase Locked Loop (PLL) and an Analog-to-Digital Converter (ADC). The paper summarizes the design from architectural as well as logic synthesis and physical design in CMOS 65nm technology.The DRAC project is co-financed by the European Union Regional Development Fund within the framework of the ERDF Operational Program of Catalonia 2014-2020 with a grant of 50% of total eligible cost. The authors are part of RedRISCV which promotes activities around open hardware. The Lagarto Project is supported by the Research and Graduate Secretary (SIP) of the Instituto Politecnico Nacional (IPN) from Mexico, and by the CONACyT scholarship for Center for Research in Computing (CIC-IPN).Peer ReviewedArticle signat per 43 autors/es: Guillem Cabo∗, Gerard Candón∗, Xavier Carril∗, Max Doblas∗, Marc Domínguez∗, Alberto González∗, Cesar Hernández†, Víctor Jiménez∗, Vatistas Kostalampros∗, Rubén Langarita∗, Neiel Leyva†, Guillem López-Paradís∗, Jonnatan Mendoza∗, Francesco Minervini∗, Julian Pavón∗, Cristobal Ramírez∗, Narcís Rodas∗, Enrico Reggiani∗, Mario Rodríguez∗, Carlos Rojas∗, Abraham Ruiz∗, Víctor Soria∗, Alejandro Suanes‡, Iván Vargas∗, Roger Figueras∗, Pau Fontova∗, Joan Marimon∗, Víctor Montabes∗, Adrián Cristal∗, Carles Hernández∗, Ricardo Martínez‡, Miquel Moretó∗§, Francesc Moll∗§, Oscar Palomar∗§, Marco A. Ramírez†, Antonio Rubio§, Jordi Sacristán‡, Francesc Serra-Graells‡, Nehir Sonmez∗, Lluís Terés‡, Osman Unsal∗, Mateo Valero∗§, Luís Villa† // ∗Barcelona Supercomputing Center (BSC), Barcelona, Spain. Email: [email protected]; †Centro de Investigación en Computación, Instituto Politécnico Nacional (CIC-IPN), Mexico City, Mexico; ‡ Institut de Microelectronica de Barcelona, IMB-CNM (CSIC), Spain. Email: [email protected]; §Universitat Politecnica de Catalunya (UPC), Barcelona, Spain. Email: [email protected] (author's final draft