Mixed regime of light-matter interaction revealed by phase sensitive measurements of the dynamical Franz-Keldysh effect

The speed of ultra-fast optical switches is generally limited by the intrinsic electronic response time of the material. Here we show that the phase content of selected electromagnetic pulses can be used to measure the timescales characteristic for the different regimes of matter-light interactions. By means of combined single cycle THz pumps and broadband optical probes, we explore the field-induced opacity in GaAs (the Franz-Keldysh effect). Our phase-resolved measurements allow to identify a novel quasi-static regime of saturation where memory effects are of relevance

Oncogenomic Approaches in Exploring Gain of Function of Mutant p53

Cancer is caused by the spatial and temporal accumulation of alterations in the genome of a given cell. This leads to the deregulation of key signalling pathways that play a pivotal role in the control of cell proliferation and cell fate. The p53 tumor suppressor gene is the most frequent target in genetic alterations in human cancers. The primary selective advantage of such mutations is the elimination of cellular wild type p53 activity. In addition, many evidences in vitro and in vivo have demonstrated that at least certain mutant forms of p53 may possess a gain of function, whereby they contribute positively to cancer progression. The fine mapping and deciphering of specific cancer phenotypes is taking advantage of molecular-profiling studies based on genome-wide approaches. Currently, high-throughput methods such as array-based comparative genomic hybridization (CGH array), single nucleotide polymorphism array (SNP array), expression arrays and ChIP-on-chip arrays are available to study mutant p53-associated alterations in human cancers. Here we will mainly focus on the integration of the results raised through oncogenomic platforms that aim to shed light on the molecular mechanisms underlying mutant p53 gain of function activities and to provide useful information on the molecular stratification of tumor patients

Manipulation of Charge Delocalization in a Bulk Heterojunction Material Using a Mid-Infrared Push Pulse

In organic bulk heterojunction materials, charge delocalization has been proposed to play a vital role in the generation of free carriers by reducing the Coulomb attraction via an interfacial charge transfer exciton (CTX). Pump-push-probe (PPP) experiments produced evidence that the excess energy given by a push pulse enhances delocalization, thereby increasing photocurrent. However, previous studies have employed near-IR push pulses in the range 0.4-0.6 eV which is larger than the binding energy of a typical CTX. This raises the doubt that the push pulse may directly promote dissociation without involving delocalized states. Here, we perform PPP experiments with mid-IR push pulses at energies that are well below the binding energy of a CTX state (0.12-0.25 eV). We identify three types of CTX: delocalized, localized, and trapped. The excitation resides over multiple polymer chains in delocalized CTXs, while is restricted to a single chain (albeit maintaining a degree of intrachain delocalization) in localized CTXs. Trapped CTXs are instead completely localized. The pump pulse generates a hot delocalized CTX, which relaxes to a localized CTX, and eventually to trapped states. We find that photo-exciting localized CTXs with push pulses resonant to the mid-IR charge transfer absorption can promote delocalization and contribute to the formation of long-lived charge separated states. On the other hand, we found that trapped CTX are non-responsive to the push pulses. We hypothesize that delocalized states identified in prior studies are only accessible in systems where there is significant interchain electronic coupling or regioregularity that supports either interchain or intrachain polaron delocalization. This emphasizes the importance of engineering the micromorphology and energetics of the donor-acceptor interface to exploit a full potential of a material for photovoltaic applications

Photon number statistics uncover the fluctuations in non-equilibrium lattice dynamics

Fluctuations of the atomic positions are at the core of a large class of unusual material properties ranging from quantum para-electricity to high temperature superconductivity. Their measurement in solids is the subject of an intense scientific debate focused on seeking a methodology capable of establishing a direct link between the variance of the atomic displacements and experimentally measurable observables. Here we address this issue by means of non-equilibrium optical experiments performed in shot-noise limited regime. The variance of the time dependent atomic positions and momenta is directly mapped into the quantum fluctuations of the photon number of the scattered probing light. A fully quantum description of the non-linear interaction between photonic and phononic fields is benchmarked by unveiling the squeezing of thermal phonons in $\alpha$-quartz.Comment: 7 pages (main text), 5 figures, 11 pages (supplementary information

Laboratory and beam test results of TOFFEE ASIC and ultra fast silicon detectors

In this report we present measurements performed on the full custom ASIC TOFFEE, designed to pre-amplify and discriminate signals of Ultra Fast Silicon Detectors. The ASIC has been characterized in laboratory with custom test boards, and with infrared laser light hitting the sensor emulating a minimum ionizing particle signal. Laser measurements show that a jitter term better than 50 (40) ps is achievable with a 10 (12) fC input charge.We also present some preliminary results on the TOFFEE performances, as obtained during recent beam tests with a 180 GeV/c pion beam, on the SPS-H8 beam line at CERN.Peer Reviewe

TP63 and TP73 in cancer, an unresolved “family” puzzle of complexity, redundancy and hierarchy

AbstractTP53 belongs to a small gene family that includes, in mammals, two additional paralogs, TP63 and TP73. The p63 and p73 proteins are structurally and functionally similar to p53 and their activity as transcription factors is regulated by a wide repertoire of shared and unique post-translational modifications and interactions with regulatory cofactors. p63 and p73 have important functions in embryonic development and differentiation but are also involved in tumor suppression. The biology of p63 and p73 is complex since both TP63 and TP73 genes are transcribed into a variety of different isoforms that give rise to proteins with antagonistic properties, the TA-isoforms that act as tumor-suppressors and DN-isoforms that behave as proto-oncogenes. The p53 family as a whole behaves as a signaling “network” that integrates developmental, metabolic and stress signals to control cell metabolism, differentiation, longevity, proliferation and death. Despite the progress of our knowledge, the unresolved puzzle of complexity, redundancy and hierarchy in the p53 family continues to represent a formidable challenge

Measurement methods for the assessment of noise impact of large vessels

The noise emissions evaluation of large vessels is often an underestimated issue. In recent years, the evolution of ship design has been characterized, both for commercial and cruise ships, by the growing tonnage and the increasing number of sound sources present on board. Consequently, the determination of acoustic emissions of sources so large and complex re-quires the development of measurement techniques that need to be always adapted to the spe-cific context of analysis and operational procedures of the vessel. Despite there being many references that can be found on technical standards, the majority of measurement methods are devoted to specific types of ships, mainly of small size, and it’s difficult to fit these evaluation procedures to large vessels. In the present paper a comparison between different measurement techniques and evaluation methods is reported, with reference to airborne noise emission in the external environment of large vessels during procedures of navigation, manoeuvring and moor-ing

Hippo and rassf1a Pathways: A Growing Affair

First discovered in Drosophila, the Hippo pathway regulates the size and shape of organ development. Its discovery and study have helped to address longstanding questions in developmental biology. Central to this pathway is a kinase cascade leading from the tumor suppressor Hippo (Mst1 and Mst2 in mammals) to the Yki protein (YAP and TAZ in mammals), a transcriptional coactivator of target genes involved in cell proliferation, survival, and apoptosis. A dysfunction of the Hippo pathway activity is frequently detected in human cancers. Recent studies have highlighted that the Hippo pathway may play an important role in tissue homoeostasis through the regulation of stem cells, cell differentiation, and tissue regeneration. Recently, the impact of RASSF proteins on Hippo signaling potentiating its proapoptotic activity has been addressed, thus, providing further evidence for Hippo's key role in mammalian tumorigenesis as well as other important diseases

The Mini-EUSO multi-level trigger algorithm and its performance

The Mini-EUSO telescope is designed by the JEM-EUSO Collaboration to observe the UV emis- sion of the Earth from the vantage point of the International Space Station in low Earth orbit. The main goal of the mission is to map the Earth in the UV, thus increasing the technological readi- ness level of future EUSO experiments and to lay the groundwork for the detection of Extreme Energy Cosmic Rays (EECRs) from space. Due to its high time resolution of 2.5 μs, Mini-EUSO is capable of detecting a wide range of UV phenomena in the Earth's atmosphere. In order to maximise the scientific return of the mission, it is necessary to implement a multi-level trigger logic for data selection on various different timescales. This logic is key to the success of the mission and thus must be thoroughly tested and integrated into the data processing system prior to launch. This article introduces the motivation behind the trigger design and details the testing of the logic through simulations and data taken at the TurLab facility