Segmentation Design of Spa Customers

Import 05/08/2014Předmětem této diplomové práce je na základně analýzy zjištěných údajů z písemného dotazníkového šetření rozdělit zákazníky lázní do skupin se stejnými či podobnými charakteristikami, chováním a názory a následně navrhnout významné segmenty zákazníků. Diplomová práce se skládá ze tří stěžejních částí. První teoreticko - metodologická část bude zaměřena na popis firmy spolu s popisem jednotlivých prvků marketingového mixu lázní. Následně se budeme v této části věnovat výkladu teoretických poznatků souvisejících s tématem práce, které budou čerpány z odborných publikací. Druhá, aplikačně - ověřovací, část bude obsahovat popis metodiky sběru dat, na kterou bude navazovat analýza segmentace zákazníků lázní. Na základě analýzy segmentačních kritérií budou vypracovány návrhy a doporučení, které tvoří třetí stěžejní část diplomové práce.The subject of this diploma thesis is - on basis of the analysis of the data gathered from the written questionnaire survey – to divide customers of spa into groups with the same or similar characteristics, behavior and attitudes and then to propose significant customers segments. The diploma thesis contains of three main parts. First theoretical - methodological part will focus on the description of the company and the description of the individual elements of the marketing mix of spa. Then this part will focus on theoretical interpretation of knowledge related to the topic of the diploma thesis, which will be gathered from professional publications. The second main part will include the describtion of the methodology of data collection, which will be followed by the segmentation analysis of the customers of spa resorts. Suggestions and recommendations will be created on the basis of analysis of segmentation criteria that form the third main part of this diploma thesis.116 - Katedra marketingu a obchoduvelmi dobř

Ontogeny and evolution of body size and sexual size dimorphism in reptiles

Monitor lizards (Varanidae) are morphologically very uniform in body shape, but much diversified in body size along both phylogenetic and ontogenetic axes. A striking sexual size dimorphism exists in monitor lizards; they are capable of fast growth, metabolism and sexual maturation. I collected the data concerning body size of particular species and verified the validity of Rench's rule, which said that there is bigger difference in body size of a conspecific male and females growing with larger body size of the species. Males are markedly bigger than females. In the next step, I focused on the model species of monitor lizards, Varanus indicus. I monitored its ontogeny very carefully. I found that this monitor lizard has pronounced sexual size dimorphism, but there are only small differences in body shape. It is capable of rapid growth and sexual maturation. The sexual dimorphism in body shape is only poor, but still measurable right in those places where the selection pressures were expected. Blood sampling monitored biochemical and haematological parameters. The concentrations of the biochemical parameters revealed the economy of resources of particular sexes partially, the costs of body growth and reproduction. Although both sexes produce the same amount of biomass (the body growth of males vs....Skupina Varanidae je tvarově velmi uniformní, ale velikostně vykazuje obrovskou variabilitu, a to jak na fylogenetické tak i na ontogenetické úrovni. Pohlavní dvojtvárnost ve velikosti (SSD) je u varanů velmi výrazná, tato skupina je schopna velmi rychlého růstu, metabolismu a pohlavního dospívání. Na základě dat o velikosti jednotlivých druhů se mi podařilo ověřit platnost Renschova pravidla, kdy s rostoucí velikostí druhu se zvětšuje rozdíl ve velikosti samců a samic, samci jsou nápadně větším pohlavím. V dalším kroku jsem se již zaměřila na modelový druh varana mangrovového (Varanus indicus). Sledovala jsem velmi detailně jeho ontogenezi. Zjistila jsem, že je tento varan poměrně značně dimorfní ve velikosti a částečně i ve tvaru těla, je schopný enormně rychlého růstu a pohlavního dospívání. Oproti velikostní pohlavní dvojtvárnosti je tvarová jen slabá, avšak měřitelná v těch místech, kde byl předpokládán selekční tlak. Odběry krve sledovaly biochemické a hematologické parametry. Koncentrace biochemických parametrů poodhalily hospodaření jednotlivých pohlaví se zdroji, nákladnost růstu a reprodukce. Ačkoliv obě pohlaví produkují obdobné množství biomasy (růst samců vs. reprodukce samic), zdá se, že investice do reprodukce jsou kvalitativně náročnější. Hodnocením hematologických parametrů jsem...Department of ZoologyKatedra zoologieFaculty of SciencePřírodovědecká fakult

Risk management in construction project delivery methods - hotel buildings

Předmětem této diplomové práce je charakteristika teoretických znalostí risk managementu, použití metod a technik řízení rizik v reálných stavebních projektech a porovnání rizik jednotlivých dodavatelských systémů použitých na tyto projekty. Řízení rizik je nedílnou součástí správného managementu, je nezbytné pro dosažení dobrého obchodu a výsledků projektu a efektivního zadávání zakázek. Tato práce je zaměřena na konkrétní stavební projekty hotelů a na řešení všech rizik, kterým tyto projekty můžou v jednotlivých fázich životního cyklu čelit. Hlavním cílem této diplomové práce je analyzovat vztah a míru rizika v jednotlivé fázi projektu k dosažení žádoucích výsledků, prostřednictvím stanovení a zavedení procesů řízení rizik, a určit metodu dodavatelských systémů, která by byla nejvhodnější a nejvýhodnější pro projekty hotelů.The task of this diploma thesis is to describe some theoretical knowledge of risk management, to apply risk management tools and techniques to real construction projects and to compare risks of possibly used delivery methods of these projects. Managing risk is an integral part of good management, and fundamental to achieving good business and project outcomes and the effective procurement of goods and services. This work is concentrated on specific projects of hotels and the handling of all risks the projects might face in each phase of the project life cycle. The main aim of this work is to analyse relation and extent of risks in each stage of project to achieve desirable results of the project by defining and implementing management processes of project risk and to determine which of the delivery methods would have been the best solution for projects of hotels

Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin Protein

INTRODUCTION: Hemojuvelin (Hjv) is a key component of the signaling cascade that regulates liver hepcidin (Hamp) expression. The purpose of this study was to determine Hjv protein levels in mice and rats subjected to iron overload and iron deficiency. METHODS: C57BL/6 mice were injected with iron (200 mg/kg); iron deficiency was induced by feeding of an iron-deficient diet, or by repeated phlebotomies. Erythropoietin (EPO)-treated mice were administered recombinant EPO at 50 U/mouse. Wistar rats were injected with iron (1200 mg/kg), or fed an iron-deficient diet. Hjv protein was determined by immunoblotting, liver samples from Hjv-/- mice were used as negative controls. Mouse plasma Hjv content was determined by a commercial ELISA kit. RESULTS: Liver crude membrane fraction from both mice and rats displayed a major Hjv-specific band at 35 kDa, and a weaker band of 20 kDa. In mice, the intensity of these bands was not changed following iron injection, repeated bleeding, low iron diet or EPO administration. No change in liver crude membrane Hjv protein was observed in iron-treated or iron-deficient rats. ELISA assay for mouse plasma Hjv did not show significant difference between Hjv+/+ and Hjv-/- mice. Liver Hamp mRNA, Bmp6 mRNA and Id1 mRNA displayed the expected response to iron overload and iron deficiency. EPO treatment decreased Id1 mRNA, suggesting possible participation of the bone morphogenetic protein pathway in EPO-mediated downregulation of Hamp mRNA. DISCUSSION: Since no differences between Hjv protein levels were found following various experimental manipulations of body iron status, the results indicate that, in vivo, substantial changes in Hamp mRNA can occur without noticeable changes of membrane hemojuvelin content. Therefore, modulation of hemojuvelin protein content apparently does not represent the limiting step in the control of Hamp gene expression

The works of "Rudolfinian composers" included in the printed book of organ tablatures Tabulaturbuch (1607)

Tématem mé diplomové práce jsou skladby obsažené v tištěné sbírce varhanních intabulací s názvem Tahulaturhuch von allerhand ausserlesnen, sch6nen, lieblichen Praeludtjs, Toccaten, Motteten, Canzonetten, Madrigalien und Fugen von 4, 5, und 6 Stimmen: dessgleichen kunstlichen Passomezzen und Gagliardenl ), která je uložena v hudebním oddělení Národní knihovny v Praze pod signaturou Pu 59 C 8985. Zaměřila jsem se především na přepis a analýzu děl skladatelů, u nichž existuje osobní vazba na Prahu v době vlády Rudolfa II., a kteří tedy bývají často označováni jako skladatelé "rudo lflnští" .Ústav hudební vědyInstitute of MusicologyFilozofická fakultaFaculty of Art

Termination of an apartment lease

Diplomová práce se zabývá skončením nájmu bytu. Práce je rozdělena do tří hlavních kapitol. Nejprve je proveden historický exkurz, v rámci kterého je pojednáno o právní úpravě skončení nájmu bytu v zákoně č. 40/1964 Sb., občanský zákoník. Dále je v práci podrobně rozebrána aktuálně účinná úprava této problematiky obsažená v zákoně č. 89/2012 Sb., občanský zákoník. Poslední část práce je zaměřena na právní úpravu skončení nájmu bytu v kanadské provincii Ontario. Ve všech částech práce je pojednáno o právech a povinnostech pronajímatele a nájemce, která těmto stranám nájemního vztahu v souvislosti se skončením nájmu bytu vznikají.ObhájenoThe thesis focuses on the termination of an apartment lease. The thesis is divided into three main chapters. First, a historical excursion is made, in which the legal regulation of the termination of the lease of an apartment in Act No. 40/1964 Coll., Civil Code, is discussed. Next, the thesis discusses in detail the currently effective regulation of this issue contained in Act No. 89/2012 Coll., Civil Code. The last part of the thesis focuses on the legal regulation of the termination of an apartment lease in the Canadian province of Ontario. All parts of the thesis deal with the rights and obligations of the landlord and tenant that arise for these parties to the tenancy relationship in connection with the termination of an apartment lease

Agricultural in terms of Sustainable Development of Agrarian Sector

The sustainability discourse clearly gives priority to the so-called "three-pillar model", according to which sustainable development should equally try to reach ecological, economic, and social goals. An increasing variety of methods is being proposed to address the question of the measurement of sustainable development. The concept of sustainable development can be interpreted in many different ways, but at its core is an approach to development that looks to balance different, and often competing, needs against an awareness of the environmental, social and economic limitations we face as asociety. Submitting theses deal with measuring sustainable agriculture and introduces a practical methodology for evaluating the sustainability of agriculture system by means of composite indicators. This methodology was based on calculating seven sustainability indicators that cover the three components of the sustainability concept (economic, social and environmental). The methodological proposal implemented in theses allowed an integrated vision of agricultural sustainability and careful selection of sustainability indicators, carried out on the basis of reliability criteria and applicability. The evaluation of agricultural sustainability/production system sustainability using the methods suggested is a potentially useful tool for public decision-makers who are tasked with designing and implementing agricultural policy. The results demonstrated the usefulness of analyzing several sustainability indicators in conjunction, in order to obtain more robust results. Such information can help to improve current agricultural policies, such as income policy, agricultural structure policy and rural development policy, with the aim of improving the sustainability of the agricultural sector

Financial Analysis for Rodinný pivovar Bernard, a. s.

Objective of this work is to undertake financial analysis and overall assessment of the financial situation of Rodinný pivovar Bernard, a. s. The theoretical part describes the methodology that is later used in the practical. The work is used publicly available information from the years 2006 to 2010

Ontogeny and evolution of body size and sexual size dimorphism in reptiles

Monitor lizards (Varanidae) are morphologically very uniform in body shape, but much diversified in body size along both phylogenetic and ontogenetic axes. A striking sexual size dimorphism exists in monitor lizards; they are capable of fast growth, metabolism and sexual maturation. I collected the data concerning body size of particular species and verified the validity of Rench's rule, which said that there is bigger difference in body size of a conspecific male and females growing with larger body size of the species. Males are markedly bigger than females. In the next step, I focused on the model species of monitor lizards, Varanus indicus. I monitored its ontogeny very carefully. I found that this monitor lizard has pronounced sexual size dimorphism, but there are only small differences in body shape. It is capable of rapid growth and sexual maturation. The sexual dimorphism in body shape is only poor, but still measurable right in those places where the selection pressures were expected. Blood sampling monitored biochemical and haematological parameters. The concentrations of the biochemical parameters revealed the economy of resources of particular sexes partially, the costs of body growth and reproduction. Although both sexes produce the same amount of biomass (the body growth of males vs. the..

Phenotypes of Saccharomyces cerevisiaecells lacking Isw2 chromatin remodelling factor

8 SUMMARY nd invasive growth. We showed that this phenotype is caused by de-repression that ncentrations of pheromone. Detailed analysis however uncovered some differences birth scar, rea that is commonly prohibited for budding. Based on our microarray data and double eletion mutant analyses, we identified one gene that is responsible for this phenotype. w2Δ cells as well as rth-scar phenotype as well as CWI pathway activation. These data prove, that de-regulation of expression of the daughter cell-specific gene DSE1 is The non-essential Saccharomyces cerevisiae protein Isw2 is a subunit of the ATP- dependent chromatin remodelling complex Isw2 that regulates the structure of chromatin, and thus plays an important role in regulation of transcription. Absence of the Isw2p induces the α-mating type-specific aberrant "shmoo-like" morphology a of a-specific genes in MATα cells and production of inappropriate a-factor which in turn activates the pheromone response pathway of isw2Δ MATα cells. Our results showed isw2Δ MATα cells can express the MATa-specific genes at the level which enables them to mate with other MATα cells. Invasion of isw2Δ MATα cells depends neither on the cell surface protein Flo11, nor on the invasive growth-specific transcription factor Tec1. On the other hand it requires components of the..