Repetibilidade e variabilidade de medições de produção de metano e dióxido de carbono em bovinos alojados em câmaras de respiração de circuito aberto

Las pruebas de recuperación de gases son necesarias cuando se emplea la técnica de cámara de respiración para medir los gases de efecto invernadero exhalados por animales domésticos. Se obtuvo un conjunto de datos de 98 mediciones individuales de producción de metano y dióxidos de carbono de ganado alojado en dos cámaras de respiración para evaluar la recolección y repetibilidad de las mediciones realizadas. Se realizó un análisis de varianza para evaluar si existían diferencias estadísticamente significativas entre cámaras y entre animales. Los resultados mostraron las ocurrencias de variaciones en la produccion de metano entre las camaras.Estas variaciones pueden deberse muy probablemente a la fuga de aire de las cámaras oa las incertidumbres en los conductos de muestra de aire y las mediciones de flujo.Se requieren experimentos de recuperación de gases cuando la técnica de cámaras de respiración es empleada para la medición de gases de efecto invernadero exhalados por los animales domésticos. Un juego de 98 mediciones de metano y dióxidos de carbono producidos por bovinos alojados en dos cámaras de respiración fueron para estimar la escasez y repetibilidad de las mediciones realizadas. Se realizó un análisis de varianza de los datos para evaluar si existían diferencias estadísticamente significativas entre las cámaras y entre los animales. Los resultados mostraron las ocurrencias de variaciones en la medicion de metano entre las camaras.Estas variaciones pueden deberse probablemente a fugas de aire de las cámaras oa la incertidumbre en el ducto de prueba de aire o en la medición del flujo de aire.Experimentos de recuperação de gases são necessários quando a técnica da câmara de respiração é used to mid gases of efeito estufa exalados by animais domestics. Um conjunto de 98 medições de metano y dióxido de carbono producido por bovinos alojados em duas câmaras de respiração foi used to estimar a consumere e repetibilidad das medições used. Uma análise de variância dos dados foi realizado para avaliar se havia diferenças estatisticamente significativas entre as câmaras e entre os animais. Os resultados muestran una ocorrência de variações na medição de metano entre as câmaras. Essas variações são provavelmente devido a vazamentos de ar das câmaras ou incerteza no duto de amostragem de ar ou na medição do fluxo de ar

Analysis of the productive and immune response of lambs infected with gastrointestinal nematodes and fed with saccharin

Objective: To determine the productive traits and immune response in Blackbelly lambs infected with gastrointestinal nematodes (GIN) fed with saccharin. Design / methodology / approach: Three treatments (T) were randomly assigned to 18 Blackbelly lambs, with an initial live weight (LW) of 13.9 + 3.2 kg. In T1 anthelmintic + base diet (CTah) was used; T2 without anthelmintic + base diet (STah) and T3 were grazing lambs without anthelmintic (STPS). The experimental design was completely randomized with measures repeated over time and the averages of the treatments were compared using Lsmeans. Evaluations included live weight (LW), egg per gram of feces (EPG), packed cell volume (PCV), total plasma protein (TPP), differential leukocyte count (WBC), and IgA level by ELISA with Haemonchus contortus and Trichostrongylus colubriformis antigens Results: The STah and CTah lambs showed a higher number of EPG (885 ± 142) and LW (29.73 ± 5.06 kg). While grazing lambs (STPS) showed the lowest PCV (26.4 ± 0.5%) compared to STah and CTah lambs (27.4 to 28.4%), caused by the high prevalence of H. contortus. The IgA level in grazing lambs was from 20.2 to 24.5% with respect to the positive standard, RPS, while the stabled lambs (STah and CTah) showed values ??close to 5% RPS. Study limitations / implications: Due to anthelmintic resistance problems, it was not possible to keep lambs free of infection in grazing, so this group was not included. Findings / conclusions: Feeding with saccharin allows increasing the resilience of sheep and achieving adequate weight gains in parasitized lambsObjective: Determine the productive and immune response of Blackbelly lambs infectedwith gastrointestinal nematodes (GIN) and fed with saccharin.Design/ methodology/ approach: A total of 18 Blackbelly lambs, with an initial live weight (LW) of 13.9 + 3.2 kg, were randomly assigned to three different treatments (T): T1, anthelmintic treatment + basal diet (CTah); T2, basal diet without anthelmintic treatment (STah); and T3, grazing lambs without anthelmintic treatment (STPS). This experiment followed a completely randomized design with repeated measures over time; mean values were compared using Lsmeans. The parameters evaluated included live weight (LW), fecal egg count per gram (FEC), packed-cell volume (PCV), plasma protein (PP), white blood celldifferential count (LEU), and IgA concentration by ELISA with Haemonchus contortusand Trichostrongylus colubriformis antigens.Results: STah and CTah lambs showed higher FEC (885 ± 142) and LW (29.73 ± 5.06kg). Grazing lambs (STPS) had lower PCV (26.4 ± 0.5%) compared to the STah andCTah lambs (27.4 to 28.4%) due to the high prevalence of H. contortus. The IgAconcentration in grazing lambs ranged from 20.2 to 24.5% of the positive standardserum titer. The feedlot lambs (STah and CTah) showed values close to 5%.Study limitations/ implications: Due to anthelmintic resistance problems, it wasimpossible to maintain grazing lambs free of infection; therefore, this group was notincluded.Findings/ conclusions: Saccharin increases sheep resilience and achieves adequateweight gains in parasitized lambs

Reduction of Enteric Methane Emissions in Heifers Fed Tropical Grass-Based Rations Supplemented with Palm Oil

Vegetable oils have been shown to reduce enteric methane (CH4) production by up to 20%. However, when the level of incorporation exceeds the threshold of 70 g/kg DM, dry matter intake (DMI) and nutrient digestibility may be reduced. The objective of this study was to determine the effects of the incorporation of three levels of palm oil (PO) on enteric CH4 emissions, rumen fermentation and apparent digestibility in heifers fed low-quality grass. Four rumen-cannulated heifers (Bos taurus × Bos indicus) were randomly assigned to four treatments: control (CON) and three increasing PO levels: 20, 40 and 60 g/kg in a 4 × 4 Latin square design with four periods of 22 days (14 days of adaptation to the ration), 5 days of feces and rumen fluid sampling (day 18, 4 h postprandial) and the last 3 days for measurements of CH4 in respiration chambers. With the exception of CP (p = 0.04), starch (p = 0.002) and EE (p < 0.001), the intake of nutrients was not affected by the inclusion of PO (p > 0.05). The apparent digestibility (AD) of nutrients was not affected by the inclusion of PO (p > 0.05), except for starch, which reduced its AD as the PO level was increased (p < 0.05). The gross energy intake was higher in PO-containing rations (p = 0.001), on the other hand, the digestible energy intake was similar between treatments (p > 0.05). In situ ruminal digestion kinetics and the potential degradability remained unchanged (p > 0.05), however, the effective degradability decreased with the inclusion of PO in the rations (p < 0.05). The ruminal pH and molar proportions of acetic, isovaleric and valeric acid were not different between treatments (p > 0.05). The ruminal concentration of propionic acid increased as the PO level increased, reaching its highest molar proportion with 60 g/kg PO (p < 0.05), however, the acetic/propionic ratio and the molar proportions of butyric acid and isobutyric acid decreased as the PO level increased (p < 0.05). The total daily CH4 production was lower in diets containing 20, 40 and 60 g/kg PO compared to the CON diet (p < 0.001). The production of CH4 per kg DMI and DOMI was greater (p < 0.05) for the CON diet compared to all three rations containing PO. The emission intensity, Ym, energy lost as CH4, emission factor (EF) and kg CO2 eq/year were reduced as an effect of the inclusion of PO (p < 0.05). Based on the results obtained, it is concluded that the incorporation of PO in cattle rations has the potential to reduce enteric methane emissions by 4% for every 10 g/kg PO in the ration, without affecting DMI, apparent digestibility or the consumption of digestible nutrient fractions

Reduction of Enteric Methane Emissions in Heifers Fed Tropical Grass-Based Rations Supplemented with Palm Oil

Vegetable oils have been shown to reduce enteric methane (CH4) production by up to 20%. However, when the level of incorporation exceeds the threshold of 70 g/kg DM, dry matter intake (DMI) and nutrient digestibility may be reduced. The objective of this study was to determine the effects of the incorporation of three levels of palm oil (PO) on enteric CH4 emissions, rumen fermentation and apparent digestibility in heifers fed low-quality grass. Four rumen-cannulated heifers (Bos taurus × Bos indicus) were randomly assigned to four treatments: control (CON) and three increasing PO levels: 20, 40 and 60 g/kg in a 4 × 4 Latin square design with four periods of 22 days (14 days of adaptation to the ration), 5 days of feces and rumen fluid sampling (day 18, 4 h postprandial) and the last 3 days for measurements of CH4 in respiration chambers. With the exception of CP (p = 0.04), starch (p = 0.002) and EE (p p > 0.05). The apparent digestibility (AD) of nutrients was not affected by the inclusion of PO (p > 0.05), except for starch, which reduced its AD as the PO level was increased (p p = 0.001), on the other hand, the digestible energy intake was similar between treatments (p > 0.05). In situ ruminal digestion kinetics and the potential degradability remained unchanged (p > 0.05), however, the effective degradability decreased with the inclusion of PO in the rations (p p > 0.05). The ruminal concentration of propionic acid increased as the PO level increased, reaching its highest molar proportion with 60 g/kg PO (p p 4 production was lower in diets containing 20, 40 and 60 g/kg PO compared to the CON diet (p 4 per kg DMI and DOMI was greater (p m, energy lost as CH4, emission factor (EF) and kg CO2 eq/year were reduced as an effect of the inclusion of PO (p < 0.05). Based on the results obtained, it is concluded that the incorporation of PO in cattle rations has the potential to reduce enteric methane emissions by 4% for every 10 g/kg PO in the ration, without affecting DMI, apparent digestibility or the consumption of digestible nutrient fractions

Epidemiology of intra-abdominal infection and sepsis in critically ill patients: "AbSeS", a multinational observational cohort study and ESICM Trials Group Project

PURPOSE: To describe the epidemiology of intra-abdominal infection in an international cohort of ICU patients according to a new system that classifies cases according to setting of infection acquisition (community-acquired, early onset hospital-acquired, and late-onset hospital-acquired), anatomical disruption (absent or present with localized or diffuse peritonitis), and severity of disease expression (infection, sepsis, and septic shock). METHODS: We performed a multicenter (n = 309), observational, epidemiological study including adult ICU patients diagnosed with intra-abdominal infection. Risk factors for mortality were assessed by logistic regression analysis. RESULTS: The cohort included 2621 patients. Setting of infection acquisition was community-acquired in 31.6%, early onset hospital-acquired in 25%, and late-onset hospital-acquired in 43.4% of patients. Overall prevalence of antimicrobial resistance was 26.3% and difficult-to-treat resistant Gram-negative bacteria 4.3%, with great variation according to geographic region. No difference in prevalence of antimicrobial resistance was observed according to setting of infection acquisition. Overall mortality was 29.1%. Independent risk factors for mortality included late-onset hospital-acquired infection, diffuse peritonitis, sepsis, septic shock, older age, malnutrition, liver failure, congestive heart failure, antimicrobial resistance (either methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, extended-spectrum beta-lactamase-producing Gram-negative bacteria, or carbapenem-resistant Gram-negative bacteria) and source control failure evidenced by either the need for surgical revision or persistent inflammation. CONCLUSION: This multinational, heterogeneous cohort of ICU patients with intra-abdominal infection revealed that setting of infection acquisition, anatomical disruption, and severity of disease expression are disease-specific phenotypic characteristics associated with outcome, irrespective of the type of infection. Antimicrobial resistance is equally common in community-acquired as in hospital-acquired infection.status: publishe

Epidemiology of intra-abdominal infection and sepsis in critically ill patients: "AbSeS", a multinational observational cohort study and ESICM Trials Group Project

Purpose To describe the epidemiology of intra-abdominal infection in an international cohort of ICU patients according to a new system that classifies cases according to setting of infection acquisition (community-acquired, early onset hospital-acquired, and late-onset hospital-acquired), anatomical disruption (absent or present with localized or diffuse peritonitis), and severity of disease expression (infection, sepsis, and septic shock). Methods We performed a multicenter (n = 309), observational, epidemiological study including adult ICU patients diagnosed with intra-abdominal infection. Risk factors for mortality were assessed by logistic regression analysis. Results The cohort included 2621 patients. Setting of infection acquisition was community-acquired in 31.6%, early onset hospital-acquired in 25%, and late-onset hospital-acquired in 43.4% of patients. Overall prevalence of antimicrobial resistance was 26.3% and difficult-to-treat resistant Gram-negative bacteria 4.3%, with great variation according to geographic region. No difference in prevalence of antimicrobial resistance was observed according to setting of infection acquisition. Overall mortality was 29.1%. Independent risk factors for mortality included late-onset hospital-acquired infection, diffuse peritonitis, sepsis, septic shock, older age, malnutrition, liver failure, congestive heart failure, antimicrobial resistance (either methicillin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, extended-spectrum beta-lactamase-producing Gram-negative bacteria, or carbapenem-resistant Gram-negative bacteria) and source control failure evidenced by either the need for surgical revision or persistent inflammation. Conclusion This multinational, heterogeneous cohort of ICU patients with intra-abdominal infection revealed that setting of infection acquisition, anatomical disruption, and severity of disease expression are disease-specific phenotypic characteristics associated with outcome, irrespective of the type of infection. Antimicrobial resistance is equally common in community-acquired as in hospital-acquired infection

Antimicrobial Lessons From a Large Observational Cohort on Intra-abdominal Infections in Intensive Care Units

evere intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by disease-specific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. In this study, we report on resistance profiles of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Enterococcus faecium in distinct European geographic regions based on an observational cohort study on intra-abdominal infections in intensive care unit (ICU) patients. Resistance against aminopenicillins, fluoroquinolones, and third-generation cephalosporins in E. coli, K. pneumoniae and P. aeruginosa is problematic, as is carbapenem-resistance in the latter pathogen. For E. coli and K. pneumoniae, resistance is mainly an issue in Central Europe, Eastern and South-East Europe, and Southern Europe, while resistance in P. aeruginosa is additionally problematic in Western Europe. Vancomycin-resistance in E. faecalis is of lesser concern but requires vigilance in E. faecium in Central and Eastern and South-East Europe. In the subcohort of patients with secondary peritonitis presenting with either sepsis or septic shock, the appropriateness of empiric antimicrobial therapy was not associated with mortality. In contrast, failure of source control was strongly associated with mortality. The relevance of these new insights for future recommendations regarding empiric antimicrobial therapy in intra-abdominal infections is discussed.Severe intra-abdominal infection commonly requires intensive care. Mortality is high and is mainly determined by diseasespecific characteristics, i.e. setting of infection onset, anatomical barrier disruption, and severity of disease expression. Recent observations revealed that antimicrobial resistance appears equally common in community-acquired and late-onset hospital-acquired infection. This challenges basic principles in anti-infective therapy guidelines, including the paradigm that pathogens involved in community-acquired infection are covered by standard empiric antimicrobial regimens, and second, the concept of nosocomial acquisition as the main driver for resistance involvement. In this study, we report on resistance profiles of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterococcus faecalis and Enterococcus faecium in distinct European geographic regions based on an observational cohort study on intra-abdominal infections in intensive care unit (ICU) patients. Resistance against aminopenicillins, fluoroquinolones, and third-generation cephalosporins in E. coli, K. pneumoniae and P. aeruginosa is problematic, as is carbapenem-resistance in the latter pathogen. For E. coli and K. pneumoniae, resistance is mainly an issue in Central Europe, Eastern and South-East Europe, and Southern Europe, while resistance in P. aeruginosa is additionally problematic in Western Europe. Vancomycin-resistance in E. faecalis is of lesser concern but requires vigilance in E. faecium in Central and Eastern and South-East Europe. In the subcohort of patients with secondary peritonitis presenting with either sepsis or septic shock, the appropriateness of empiric antimicrobial therapy was not associated with mortality. In contrast, failure of source control was strongly associated with mortality. The relevance of these new insights for future recommendations regarding empiric antimicrobial therapy in intra-abdominal infections is discussed

Poor timing and failure of source control are risk factors for mortality in critically ill patients with secondary peritonitis

Purpose: To describe data on epidemiology, microbiology, clinical characteristics and outcome of adult patients admitted in the intensive care unit (ICU) with secondary peritonitis, with special emphasis on antimicrobial therapy and source control. Methods: Post hoc analysis of a multicenter observational study (Abdominal Sepsis Study, AbSeS) including 2621 adult ICU patients with intra-abdominal infection in 306 ICUs from 42 countries. Time-till-source control intervention was calculated as from time of diagnosis and classified into 'emergency' (&lt; 2 h), 'urgent' (2-6 h), and 'delayed' (&gt; 6 h). Relationships were assessed by logistic regression analysis and reported as odds ratios (OR) and 95% confidence interval (CI). Results: The cohort included 1077 cases of microbiologically confirmed secondary peritonitis. Mortality was 29.7%. The rate of appropriate empiric therapy showed no difference between survivors and non-survivors (66.4% vs. 61.3%, p = 0.1). A stepwise increase in mortality was observed with increasing Sequential Organ Failure Assessment (SOFA) scores (19.6% for a value ≤ 4-55.4% for a value &gt; 12, p &lt; 0.001). The highest odds of death were associated with septic shock (OR 3.08 [1.42-7.00]), late-onset hospital-acquired peritonitis (OR 1.71 [1.16-2.52]) and failed source control evidenced by persistent inflammation at day 7 (OR 5.71 [3.99-8.18]). Compared with 'emergency' source control intervention (&lt; 2 h of diagnosis), 'urgent' source control was the only modifiable covariate associated with lower odds of mortality (OR 0.50 [0.34-0.73]). Conclusion: 'Urgent' and successful source control was associated with improved odds of survival. Appropriateness of empirical antimicrobial treatment did not significantly affect survival suggesting that source control is more determinative for outcome

International Nosocomial Infection Control Consortiu (INICC) report, data summary of 43 countries for 2007-2012. Device-associated module

We report the results of an International Nosocomial Infection Control Consortium (INICC) surveillance study from January 2007-December 2012 in 503 intensive care units (ICUs) in Latin America, Asia, Africa, and Europe. During the 6-year study using the Centers for Disease Control and Prevention's (CDC) U.S. National Healthcare Safety Network (NHSN) definitions for device-associated health care–associated infection (DA-HAI), we collected prospective data from 605,310 patients hospitalized in the INICC's ICUs for an aggregate of 3,338,396 days. Although device utilization in the INICC's ICUs was similar to that reported from ICUs in the U.S. in the CDC's NHSN, rates of device-associated nosocomial infection were higher in the ICUs of the INICC hospitals: the pooled rate of central line–associated bloodstream infection in the INICC's ICUs, 4.9 per 1,000 central line days, is nearly 5-fold higher than the 0.9 per 1,000 central line days reported from comparable U.S. ICUs. The overall rate of ventilator-associated pneumonia was also higher (16.8 vs 1.1 per 1,000 ventilator days) as was the rate of catheter-associated urinary tract infection (5.5 vs 1.3 per 1,000 catheter days). Frequencies of resistance of Pseudomonas isolates to amikacin (42.8% vs 10%) and imipenem (42.4% vs 26.1%) and Klebsiella pneumoniae isolates to ceftazidime (71.2% vs 28.8%) and imipenem (19.6% vs 12.8%) were also higher in the INICC's ICUs compared with the ICUs of the CDC's NHSN