Gekeimte Samen als Futtermittel - Analytik

Bis August 2005 dürfen im ökologischen Landbau, wenn eine ausschließliche Versorgung mit ökologischen Futtermitteln nicht möglich ist, im begrenzten Umfang konventionelle zugesetzt werden. Für den Zeitraum danach ist zu klären, ob eine ausreichende Nährstoff- und Eiweißversorgung über den Einsatz von Getreidekeimlingen gewährleistet werden kann. Die während des Keimprozesses bei Getreide auftretenden Veränderungen der für die Fütterung relevanten Inhaltsstoffe wurden untersucht. Die Keimung erfolgte sowohl unter optimierten Bedingungen in Feuchtekammern als auch unter praxisrelevanten Bedingungen in Schalen und im Keimautomat. Dabei zeigte sich, dass mit beginnender Keimung sprunghafte Veränderungen im Enzymstatus nachweisbar sind, während stoffliche Veränderungen später einsetzen und langsamer verlaufen. Das stärkeabbauende Enzym a-Amylase konnte als sensibler Indikator für den Keimungsfortschritt verwendet werden. Während der Keimung stiegen die Aktivitäten der stärkeabbauenden Enzyme und der Stärkegehalt wurde reduziert. Erwartungsgemäß stiegen die Zuckergehalte. Bei unverändertem Rohstickstoffgehalt kam es zu einer Abnahme des Proteinstickstoffgehaltes zu Gunsten der freien Aminosäuren. Der Rohfettgehalt stieg und es erhöhte sich der Gehalt an mehrfach ungesättigten Fettsäuren. Von den Aminosäuren erhöhte sich während der Keimung besonders der Gehalt von Lysin. Die deutlichsten Veränderungen durch die Keimung wurden bei den Vitaminen beobachtet. Von den 8 untersuchten Vitaminen A, B1, B2, B6, C, D3, E und K1 stiegen 6 deutlich an. Bemerkenswert war die Verringerung der Viskosität in Roggenkeimlingen, wodurch der Einsatz dieser Getreideart in der Fütterung interessant wird. Erwähnenswert ist, dass die Phytinsäure, die die Verdaulichkeit des Futters beeinträchtigt, während der Keimung stark abnimmt. Aus ernährungsphysiologischer Sicht treten vorteilhafte Veränderungen während der Keimung auf, die zu einer Verbesserung des Futterwertes beitragen

Einsatz von gekeimtem Getreide als Futtermittel

Die EU-Verordnung 1804/1999 regelt die Einbeziehung der tierischen Erzeugung in den Geltungsbereich der Verordnung (EWG) Nr. 2092/91 über den ökologischen Landbau und die entsprechende Kennzeichnung der landwirtschaftlichen Erzeugnisse und Lebensmittel. Danach müssen im ökologischen Landbau alle Tiere nach den Grundregeln dieser Verordnung gehalten werden. Das Futter soll den ernährungsphysiologischen Bedarf der Tiere decken und aus dem ökologischen Anbau stammen. Dafür dürfen bis August 2005 im begrenzten Umfang konventionelle Futtermittel zugesetzt werden, wenn eine ausschließliche Versorgung mit Futtermitteln aus dem ökologischen Anbau nicht möglich ist. Für die Geflügelfütterung bedeutet das einen zulässigen Höchstanteil an konventionellem Futter von 20% im Jahr (max. 25% Trockenmasse am Tag). Bislang werden dafür besonders die konventionellen Komponenten Maiskleber und Kartoffeleiweiß, die als Nebenprodukte bei der Stärkegewinnung anfallen, eingesetzt. Vergleichbare ökologische Produkte sind z. Z. nicht verfügbar. Unter diesem Aspekt ist zu klären, ob eine ausreichende Nährstoff- und Eiweißversorgung über den Einsatz von 20% Getreidekeimlingen in der Futterration gewährleistet werden kann, die damit zu 100% ökologischer Herkunft ist. In einem, im Rahmen des Bundesprogramms ökologischer Landbau, geförderten Projekt werden alle nachfolgend aufgeführten Parameter analysiert. Proteine, Stärken, Nichtstärkepolysaccharide, Zucker und Fette als wertgebende Inhaltsstoffe sowie Auswuchs, Pilzbefall und Mykotoxine als dominierende Schadfaktoren in Getreide stehen dabei im Mittelpunkt des Interesses. Ziel ist es, Kriterien für die optimale Prozessführung der Keimung und eine hohe Produktqualität der Keimlinge zu sichern, um eine hochwertige Futterkomponente aus dem ökologischen Landbau für die Tierernährung bereitzustellen

Human helminth therapy to treat inflammatory disorders - where do we stand?

Parasitic helminths have evolved together with the mammalian immune system over many millennia and as such they have become remarkably efficient modulators in order to promote their own survival. Their ability to alter and/or suppress immune responses could be beneficial to the host by helping control excessive inflammatory responses and animal models and pre-clinical trials have all suggested a beneficial effect of helminth infections on inflammatory bowel conditions, MS, asthma and atopy. Thus, helminth therapy has been suggested as a possible treatment method for autoimmune and other inflammatory disorders in humans

Time-Action Analysis (TAA) of the Surgical Technique Implanting the Collum Femoris Preserving (CFP) Hip Arthroplasty. TAASTIC trial Identifying pitfalls during the learning curve of surgeons participating in a subsequent randomized controlled trial (An observational study)

<p>Abstract</p> <p>Background</p> <p>Two types of methods are used to assess learning curves: outcome assessment and process assessment. Outcome measures are usually dichotomous rare events like complication rates and survival or require an extensive follow-up and are therefore often inadequate to monitor individual learning curves. Time-action analysis (TAA) is a tool to objectively determine the level of efficiency of individual steps of a surgical procedure.</p> <p>Methods/Design</p> <p>We are currently using TAA to determine the number of cases needed for surgeons to reach proficiency with a new innovative hip implant prior to initiating a multicentre RCT. By analysing the unedited video recordings of the first 20 procedures of each surgeon the number and duration of the actions needed for a surgeon to achieve his goal and the efficiency of these actions is measured. We constructed a taxonomy or list of actions which together describe the complete surgical procedure. In the taxonomy we categorised the procedure in 5 different Goal Oriented Phases (GOP):</p> <p>1. the incision phase</p> <p>2. the femoral phase</p> <p>3. the acetabulum phase</p> <p>4. the stem phase</p> <p>5. the closure pase</p> <p>Each GOP was subdivided in Goal Oriented Actions (GOA) and each GOA is subdivided in Separate Actions (SA) thereby defining all the necessary actions to complete the procedure. We grouped the SAs into GOAs since it would not be feasible to measure each SA. Using the video recordings, the duration of each GOA was recorded as well as the amount of delay. Delay consists of repetitions, waiting and additional actions. The nett GOA time is the total GOA time – delay and is a representation of the level of difficulty of each procedure. Efficiency is the percentage of nett GOA time during each procedure.</p> <p>Discussion</p> <p>This allows the construction of individual learning curves, assessment of the final skill level for each surgeon and comparison of different surgeons prior to participation in an RCT. We believe an objective and comparable assessment of skill level by process assessment can improve the value of a surgical RCT in situations where a learning curve is expected.</p

Association of Genetic Variants With Primary Open-Angle Glaucoma Among Individuals With African Ancestry.

Importance:Primary open-angle glaucoma presents with increased prevalence and a higher degree of clinical severity in populations of African ancestry compared with European or Asian ancestry. Despite this, individuals of African ancestry remain understudied in genomic research for blinding disorders. Objectives:To perform a genome-wide association study (GWAS) of African ancestry populations and evaluate potential mechanisms of pathogenesis for loci associated with primary open-angle glaucoma. Design, Settings, and Participants:A 2-stage GWAS with a discovery data set of 2320 individuals with primary open-angle glaucoma and 2121 control individuals without primary open-angle glaucoma. The validation stage included an additional 6937 affected individuals and 14 917 unaffected individuals using multicenter clinic- and population-based participant recruitment approaches. Study participants were recruited from Ghana, Nigeria, South Africa, the United States, Tanzania, Britain, Cameroon, Saudi Arabia, Brazil, the Democratic Republic of the Congo, Morocco, Peru, and Mali from 2003 to 2018. Individuals with primary open-angle glaucoma had open iridocorneal angles and displayed glaucomatous optic neuropathy with visual field defects. Elevated intraocular pressure was not included in the case definition. Control individuals had no elevated intraocular pressure and no signs of glaucoma. Exposures:Genetic variants associated with primary open-angle glaucoma. Main Outcomes and Measures:Presence of primary open-angle glaucoma. Genome-wide significance was defined as P &lt; 5 × 10-8 in the discovery stage and in the meta-analysis of combined discovery and validation data. Results:A total of 2320 individuals with primary open-angle glaucoma (mean [interquartile range] age, 64.6 [56-74] years; 1055 [45.5%] women) and 2121 individuals without primary open-angle glaucoma (mean [interquartile range] age, 63.4 [55-71] years; 1025 [48.3%] women) were included in the discovery GWAS. The GWAS discovery meta-analysis demonstrated association of variants at amyloid-β A4 precursor protein-binding family B member 2 (APBB2; chromosome 4, rs59892895T&gt;C) with primary open-angle glaucoma (odds ratio [OR], 1.32 [95% CI, 1.20-1.46]; P = 2 × 10-8). The association was validated in an analysis of an additional 6937 affected individuals and 14 917 unaffected individuals (OR, 1.15 [95% CI, 1.09-1.21]; P &lt; .001). Each copy of the rs59892895*C risk allele was associated with increased risk of primary open-angle glaucoma when all data were included in a meta-analysis (OR, 1.19 [95% CI, 1.14-1.25]; P = 4 × 10-13). The rs59892895*C risk allele was present at appreciable frequency only in African ancestry populations. In contrast, the rs59892895*C risk allele had a frequency of less than 0.1% in individuals of European or Asian ancestry. Conclusions and Relevance:In this genome-wide association study, variants at the APBB2 locus demonstrated differential association with primary open-angle glaucoma by ancestry. If validated in additional populations this finding may have implications for risk assessment and therapeutic strategies

The Noise Exposure Structured Interview (NESI): an instrument for the comprehensive estimation of lifetime noise exposure

Lifetime noise exposure is generally quantified by self report. The accuracy of retrospective self report is limited by respondent recall, but is also bound to be influenced by reporting procedures. Such procedures are of variable quality in current measures of lifetime noise exposure, and off-the-shelf instruments are not readily available. The Noise Exposure Structured Interview (NESI) represents an attempt to draw together some of the stronger elements of existing procedures and to provide solutions to their outstanding limitations. Reporting is not restricted to pre-specified exposure activities, and instead encompasses all activities that the respondent has experienced as noisy (defined based on sound level estimated from vocal effort). Changing exposure habits over time are reported by dividing the lifespan into discrete periods in which exposure habits were approximately stable, with life milestones used to aid recall. Exposure duration, sound level, and use of hearing protection are reported for each life period separately. Simple-to-follow methods are provided for the estimation of free-field sound level, the sound level emitted by personal listening devices, and the attenuation provided by hearing protective equipment. An energy-based means of combining the resulting data is supplied, along with a primarily energy-based method for incorporating firearm-noise exposure. Finally, the NESI acknowledges the need of some users to tailor the procedures; this flexibility is afforded and reasonable modifications are described. Competency needs of new users are addressed through detailed interview instructions (including troubleshooting tips) and a demonstration video. Limited evaluation data are available and future efforts at evaluation are proposed

Retinoic Acid Mediates Regulation of Network Formation by COUP-TFII and VE-Cadherin Expression by TGFβ Receptor Kinase in Breast Cancer Cells

Tumor development, growth, and metastasis depend on the provision of an adequate vascular supply. This can be due to regulated angiogenesis, recruitment of circulating endothelial progenitors, and/or vascular transdifferentiation. Our previous studies showed that retinoic acid (RA) treatment converts a subset of breast cancer cells into cells with significant endothelial genotypic and phenotypic elements including marked induction of VE-cadherin, which was responsible for some but not all morphological changes. The present study demonstrates that of the endothelial-related genes induced by RA treatment, only a few were affected by knockdown of VE-cadherin, ruling it out as a regulator of the RA-induced endothelial genotypic switch. In contrast, knockdown of the RA-induced gene COUP-TFII prevented the formation of networks in Matrigel but had no effect on VE-cadherin induction or cell fusion. Two pan-kinase inhibitors markedly blocked RA-induced VE-cadherin expression and cell fusion. However, RA treatment resulted in a marked and broad reduction in tyrosine kinase activity. Several genes in the TGFβ signaling pathway were induced by RA, and specific inhibition of the TGFβ type I receptor blocked both RA-induced VE-cadherin expression and cell fusion. Together these data indicate a role for the TGFβ pathway and COUP-TFII in mediating the endothelial transdifferentiating properties of RA