217 research outputs found
Going deeper into the toxicokinetics of synthetic cannabinoids: in vitro contribution of human carboxylesterases
Synthetic cannabinoids (SC) are new psychoactive substances known to cause intoxications and fatalities. One reason may be the limited data available concerning the toxicokinetics of SC, but toxicity mechanisms are insufficiently understood so far. Human carboxylesterases (hCES) are widely known to play a crucial role in the catalytic hydrolysis of drugs (of abuse). The aim of this study was to investigate the in vitro contribution of hCES to the metabolism of the 13 SC 3,5-AB-5F-FUPPYCA, AB-5F-P7AICA, A-CHMINACA, DMBA-CHMINACA, MBA-CHMINACA, MDMB-4F-BINACA, MDMB-4en-PINACA, MDMB-FUBICA, MDMB-5F-PICA, MMB-CHMICA, MMB-4en-PICA, MMB-FUBINACA, and MPhP-5F-PICA. The SC were incubated with recombinant hCES1b, hCES1c, or hCES2 and analyzed by liquid chromatography–ion trap mass spectrometry to assess amide or ester hydrolysis in an initial activity screening. Enzyme kinetic studies were performed if sufficient hydrolysis was observed. No hydrolysis of the amide linker was observed using those experimental conditions. Except for MDMB-5F-PICA, ester hydrolysis was always detected if an ester group was present in the head group. In general, SC with a terminal ester bearing a small alcohol part and a larger acyl part showed higher affinity to hCES1 isozymes. Due to the low hydrolysis rates, enzyme kinetics could not be modeled for the SC with a tert-leucine-derived moiety, but hydrolysis reactions of MPhP-5F-PICA and of those containing a valine-derived moiety followed classic Michaelis–Menten kinetics. In conclusion, drug–drug/drug–food interactions or hCES polymorphisms may prolong the half-life of SC and the current results help to estimate the risk of toxicity in the future after combining them with activity and clinical data
Comparison of Three Untargeted Data Processing Workflows for Evaluating LC-HRMS Metabolomics Data
The evaluation of liquid chromatography high-resolution mass spectrometry (LC-HRMS)
raw data is a crucial step in untargeted metabolomics studies to minimize false positive findings.
A variety of commercial or open source software solutions are available for such data processing.
This study aims to compare three different data processing workflows (Compound Discoverer 3.1,
XCMS Online combined with MetaboAnalyst 4.0, and a manually programmed tool using R) to
investigate LC-HRMS data of an untargeted metabolomics study. Simple but highly standardized
datasets for evaluation were prepared by incubating pHLM (pooled human liver microsomes)
with the synthetic cannabinoid A-CHMINACA. LC-HRMS analysis was performed using normaland reversed-phase chromatography followed by full scan MS in positive and negative mode.
MS/MS spectra of significant features were subsequently recorded in a separate run. The outcome of
each workflow was evaluated by its number of significant features, peak shape quality, and the results
of the multivariate statistics. Compound Discoverer as an all-in-one solution is characterized by its
ease of use and seems, therefore, suitable for simple and small metabolomic studies. The two open
source solutions allowed extensive customization but particularly, in the case of R, made advanced
programming skills necessary. Nevertheless, both provided high flexibility and may be suitable for
more complex studies and questions
Addendum: Hemmer, S., et al. Comparison of Three Untargeted Data Processing Workflows for Evaluating LC-HRMS Metabolomics Data. Metabolites 2020, 10, 378
The authors wish to make the following comment to this paper [...
How to Study the Metabolism of New Psychoactive Substances for the Purpose of Toxicological Screenings — A Follow-Up Study Comparing Pooled Human Liver S9, HepaRG Cells, and Zebrafish Larvae
The new psychoactive substances (NPS) market continues to be very dynamic. A large number of compounds belonging to diverse chemical groups continue to emerge. This makes their detection in biological samples challenging for clinical and forensic toxicologists. Knowledge of the metabolic fate of NPS is crucial for developing comprehensive screening procedures. As human studies are not feasible due to ethical concerns, the current study aimed to compare the NPS' metabolic pattern in incubations with pooled human liver S9 fraction (pHLS9), human liver HepaRG cells, and zebrafish larvae. The latter model was recently shown to be a promising preclinical surrogate for human hepatic metabolism of a synthetic cannabinoid. However, studies concerning other NPS classes are still missing and therefore an amphetamine-based N-methoxybenzyl (NBOMe) compound, a synthetic cathinone, a pyrrolidinophenone analog, a lysergamide, as well as another synthetic cannabinoid were included in the current study. Liquid chromatography coupled to Orbitrap-based high-resolution tandem mass spectrometry was used to analyze metabolic data. Zebrafish larvae were found to produce the highest number of phase I but also phase II metabolites (79 metabolites in total), followed by HepaRG cells (66 metabolites). Incubations with pHLS9 produced the least metabolites (57 metabolites). Furthermore, the involvement of monooxygenases and esterases in the metabolic phase I transformations of 4F-MDMB-BINACA was elucidated using single-enzyme incubations. Several cytochrome P450 (CYP) isozymes were shown to contribute, and CYP3A5 was involved in all CYP-catalyzed reactions, while amide and ester hydrolysis were catalyzed by the human carboxylesterase (hCES) isoforms hCES1b and/or hCES1c. Finally, metabolites were compared to those present in human biosamples if data were available. Overall, the metabolic patterns in HepaRG cells provided the worst overlap with that in human biosamples. Zebrafish larvae experiments agreed best with data found in human plasma and urine analysis. The current study underlines the potential of zebrafish larvae as a tool for elucidating the toxicokinetics of NPS in the future
Überblick über die neueren Arbeiten auf dem Gebiet des Wasserstoff-und Tritiumverhaltens in Hochtemperaturreaktoren
This report comprises the contributions of members of the "Institut für Reaktor-Entwicklung" (IRE) to the "Und Seminar on Hydrogen and Tritium Behaviour in High Temperature Reactors", which was held March 8, 1978, at KFA Jülich. At the beginning the problem is introduced and the investigations at IRE related to this area are presented in their context. Then follow the individual papers on the subjects mentioned. At first the experiences with the operation of the experimental facility AUWARM and the newest results in the current testing program are discussed. Therafter the model investigations with hydrogen and deuterium on the problem of hydrogen- and tritium permeation are reported and a computer program for balancing tritium in pebble-bed-HTRs is described. Last notleast the studies on the behaviour of tritium in matrix graphite and the experiments on primary coolant purification by titanium gettering are shortly communicated. The results given in this report are preliminary informations on the actual status of the current investigations
The Influence of Board Structure and Ownership Concentration on GRI Reporting
Purpose – The purpose of this paper is to investigate the relationship
between corporate governance structure and GRI reporting. More
specifically, the study seeks to analyse board independence, board
size and ownership concentration and their relationships with GRI
reporting.
Design/methodology/approach – The hypotheses of the study were
tested in a sample of 287 Brazilian companies listed on the B3, the
Brazilian stock exchange, using logistic regression models. Data from
2013 were collected from the Econoinfo and GRI databases.
Findings –The findings show that there is a positive relationship
between both board independence and GRI reporting and board size
and GRI reporting, and a neutral relationship between ownership
concentration and GRI reporting. These results indicate that the
corporate governance structure influences a company’s decision to
engage in social issue and stakeholders’ relationship activities.
Originality/value – The contribution of this study is it presents
theoretical arguments and empirical evidence regarding the influence
of corporate governance structure on CSD beyond the Anglo-Saxon
context. The results show that good corporate governance practices
cannot be generalized to different contexts
Preorganized tridentate analogues of mixed hydroxyoxime/carboxylate nickel extractants
Simple tridentate ligands can operate as Ni-extractants in the pH-dependent process: 2LHorg + NiSO4 ⇌ [(L)2Ni]org + H2SO4.</p
Chemical Genetic Analysis and Functional Characterization of Staphylococcal Wall Teichoic Acid 2-Epimerases Reveals Unconventional Antibiotic Drug Targets
Here we describe a chemical biology strategy performed in Staphylococcus aureus and Staphylococcus epidermidis to identify MnaA, a 2-epimerase that we demonstrate interconverts UDP-GlcNAc and UDP-ManNAc to modulate substrate levels of TarO and TarA wall teichoic acid (WTA) biosynthesis enzymes. Genetic inactivation of mnaA results in complete loss of WTA and dramatic in vitro β-lactam hypersensitivity in methicillin-resistant S. aureus (MRSA) and S. epidermidis (MRSE). Likewise, the β-lactam antibiotic imipenem exhibits restored bactericidal activity against mnaA mutants in vitro and concomitant efficacy against 2-epimerase defective strains in a mouse thigh model of MRSA and MRSE infection. Interestingly, whereas MnaA serves as the sole 2-epimerase required for WTA biosynthesis in S. epidermidis, MnaA and Cap5P provide compensatory WTA functional roles in S. aureus. We also demonstrate that MnaA and other enzymes of WTA biosynthesis are required for biofilm formation in MRSA and MRSE. We further determine the 1.9Å crystal structure of S. aureus MnaA and identify critical residues for enzymatic dimerization, stability, and substrate binding. Finally, the natural product antibiotic tunicamycin is shown to physically bind MnaA and Cap5P and inhibit 2-epimerase activity, demonstrating that it inhibits a previously unanticipated step in WTA biosynthesis. In summary, MnaA serves as a new Staphylococcal antibiotic target with cognate inhibitors predicted to possess dual therapeutic benefit: as combination agents to restore β-lactam efficacy against MRSA and MRSE and as non-bioactive prophylactic agents to prevent Staphylococcal biofilm formation.publishe
Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins
Physarum polycephalum expresses two closely related, calcium-independent NOSs (nitric oxide synthases). In our previous work, we showed that both NOSs are induced during starvation and apparently play a functional role in sporulation. In the present study, we characterized the genomic structures of both Physarum NOSs, expressed both enzymes recombinantly in bacteria and characterized their biochemical properties. Whereas the overall genomic organization of Physarum NOS genes is comparable with various animal NOSs, none of the exon–intron boundaries are conserved. Recombinant expression of clones with various N-termini identified N-terminal amino acids essential for enzyme activity, but not required for haem binding or dimerization, and suggests the usage of non-AUG start codons for Physarum NOSs. Biochemical characterization of the two Physarum isoenzymes revealed different affinities for L-arginine, FMN and 6R-5,6,7,8-tetrahydro-L-biopterin
- …