Digital workflow for the acquisition and elaboration of 3D data in a monumental complex: the fortress of Saint John the Baptist in Florence

In recent years, the GeCo Laboratory has undertaken numerous projects to digitalize vast and complex buildings; the specific nature of the different projects has resulted in a case-by-case approach, each time working on past experiences and updating not only the hardware and software tools but also the management and processing methods. This paper presents the workflow followed for the survey of the Fortress of Saint John the Baptist in Florence, an on-going interdisciplinary project. Presently Florence’s main trade fair congress centre, at the same time it hosts various buildings that bear witness to the fortress’s life-history, combining constructions from the Medici and Lorraine eras with recently built exhibition facilities. Now new research has been required due to the realization of new pavilions and the regeneration of the whole complex. This has included a critical survey, material testing, diagnostic investigations and stratigraphic analyses to define the building’s state of preservation. The working group comprises specialists from different institutions, amongst which the Italian Military Geographic Institute, the University of Florence, the National Research Council Institute for the Preservation and Enhancement of the Cultural Heritage, and the Florence City Council

The florence baptistery: 3-D Survey as a knowledge tool for historical and structural investigations

The Baptistery of San Giovanni is one of the most important pieces of architecture in Florence. It is an octagonal building, encrusted with marble both internally and externally (including the pyramidal roof) and covered inside by a magnificent dome with sparkling gold mosaics. During Dante’s time, it appeared much older than the other monuments, so its origins were considered as hailing straight from Florence’s most remote and mythical history. Even though we have much more data now, scholars still disagree over the interpretations on the origin and construction sequence of the monument. Survey has always been considered a main instrument for understanding historical architecture, mostly from constructional and structural points of view. During the last century, the Baptistery was surveyed using both traditional techniques and the most up-to-date instruments available at the time, such as topography, close-range photogrammetry and laser scanning. So, a review of those early applications, even if partial or isolated, can significantly attest to the state of the art and evolution of survey techniques. During recent years, the Opera di Santa Maria del Fiore promoted new research and a wide range of diagnostic investigations aimed at acquiring greater knowledge of the monument in anticipation of the cleaning and restoration of the outer wall surfaces during 2015. Among this research, GeCo Lab carried out a new systematic and complete laser scanner survey of the whole Baptistery, acquiring data for the more inaccessible parts that were given little attention during other survey campaigns. First of all, the paper analyses recent contributions given by instrumental surveys in advancing knowledge of the building, with references to the cutting-edge techniques and measurement tools used at the time. Then, it describes the new survey campaign, illustrating the approach followed in the planning, data acquisition and data elaboration phases; finally, it gives examples of some interpretations of the structure stemming from the new acquisitions

HIGH-QUALITY 3D MODELS AND THEIR USE IN A CULTURAL HERITAGE CONSERVATION PROJECT

Cultural heritage digitization and 3D modelling processes are mainly based on laser scanning and digital photogrammetry techniques to produce complete, detailed and photorealistic three-dimensional surveys: geometric as well as chromatic aspects, in turn testimony of materials, work techniques, state of preservation, etc., are documented using digitization processes. The paper explores the topic of 3D documentation for conservation purposes; it analyses how geomatics contributes in different steps of a restoration process and it presents an overview of different uses of 3D models for the conservation and enhancement of the cultural heritage. The paper reports on the project to digitize the earthenware frieze of the Ospedale del Ceppo in Pistoia (Italy) for 3D documentation, restoration work support, and digital and physical reconstruction and integration purposes. The intent to design an exhibition area suggests new ways to take advantage of 3D data originally acquired for documentation and scientific purposes

Laser scanning and modelling of barely visible features: the survey of the Grotto of the Animals at the Villa of Castello (Florence)

The deep fusion of natural and artificial elements typical of Italian Renaissance gardens is particularly evident in the park of Villa di Castello and in the Grotto of the Animals, also called Grotto of the Flood. The soil slope is the essential element of a huge underlying hydraulic machine and it is the result of extensive earthworks which led to the construction of the big retaining wall limiting the grotto and the adjacent fountains. Hence, this grotto represents only the visible part of a mechanism running all around it. It is formed by a single chamber vaulted and covered with sponge-like stones, as well as decorations made of pebbles and shells. The space is divided into three wings, with big marble basins at their end. Over them there are reliefs of animals made of different stones and marbles. Animals recur also in the compositions of fish and shellfish decorating the side basins and in the bronze birds currently kept in the Museo del Bargello. The name “Grotto of the Flood” comes from the water feature that characterised this place: visitors were surprised by tens of jets hidden among the stones in the vault and in the floor. To obtain this effect, the whole grotto is surrounded by multi-storey tunnels, hiding the hydraulic system and people activating the mechanisms. Research agreements were drawn up between the Special Superintendence for the Historical, Artistic and Ethnoanthropological Heritage, the Florence museums group and the GeCO Lab, for the realization of the survey presented in this paper. The task of the GeCO Lab was thus identifying the best solutions to check the spatial relations between the grotto and the area above, as well as the geometric and functional connections between the building and the ancient hydraulic system, composed by pipes and nozzles concealed between the stones. Besides, the overall survey was intended as a documentation of the on-going restoration work

Newer agents for Helicobacter pylori eradication

Helicobacter pylori infection remains widespread internationally, with a definite morbidity and mortality. The efficacy of standard 7–14 day triple therapies is decreasing, mainly due to increasing primary bacterial resistance to antibiotics. Currently, the most effective treatments are either the sequential regimen or the concomitant therapy. Different patents have been registered showing high bactericidal effects in vitro, some of which are active against clarithromycin- and metronidazole-resistant strains, even at low pH values. Among these novel molecules, benzimidazole-derivatives, polycyclic compounds, pyloricidin, and arylthiazole analogues seem to be the more promising. The identification of essential genes for either bacterial colonization or growth represents a route for potential target therapies in the near future

Rapamycin rescues mitochondrial dysfunction in cells carrying the m.8344A > G mutation in the mitochondrial tRNALys

Background: Myoclonus, Epilepsy and Ragged-Red-Fibers (MERRF) is a mitochondrial encephalomyopathy due to heteroplasmic mutations in mitochondrial DNA (mtDNA) most frequently affecting the tRNALys gene at position m.8344A > G. Defective tRNALys severely impairs mitochondrial protein synthesis and respiratory chain when a high percentage of mutant heteroplasmy crosses the threshold for full-blown clinical phenotype. Therapy is currently lim- ited to symptomatic management of myoclonic epilepsy, and supportive measures to counteract muscle weakness with co-factors/supplements. Methods: We tested two therapeutic strategies to rescue mitochondrial function in cybrids and fibroblasts carry- ing different loads of the m.8344A > G mutation. The first strategy was aimed at inducing mitochondrial biogenesis directly, over-expressing the master regulator PGC-1α, or indirectly, through the treatment with nicotinic acid, a NAD+ precursor. The second was aimed at stimulating the removal of damaged mitochondria through prolonged rapamy- cin treatment. Results: The first approach slightly increased mitochondrial protein expression and respiration in the wild type and intermediate-mutation load cells, but was ineffective in high-mutation load cell lines. This suggests that induction of mitochondrial biogenesis may not be sufficient to rescue mitochondrial dysfunction in MERRF cells with high-muta- tion load. The second approach, when administered chronically (4 weeks), induced a slight increase of mitochondrial respiration in fibroblasts with high-mutation load, and a significant improvement in fibroblasts with intermediate- mutation load, rescuing completely the bioenergetics defect. This effect was mediated by increased mitochondrial biogenesis, possibly related to the rapamycin-induced inhibition of the Mechanistic Target of Rapamycin Complex 1 (mTORC1) and the consequent activation of the Transcription Factor EB (TFEB). Conclusions: Overall, our results point to rapamycin-based therapy as a promising therapeutic option for MERRF

Encapsulation of cationic iridium(iii) tetrazole complexes into a silica matrix: Synthesis, characterization and optical properties

Herein we report the easy incorporation of brightly phosphorescent cationic iridium(iii) tetrazole complexes into a silica based matrix via an easily scalable colloidal process. For this purpose, two cationic Ir(iii) emitters bearing 5-aryl tetrazole ligands (R-CN4) were selected: blue [F2IrPTZ-Me]+(C^N = F2ppy; N^N = PTZ-Me-2-(2-methyl-2H-tetrazol-5-yl)pyridine) and red [IrQTZ-Me]+(C^N = ppy; N^N = QTZ-Me-2-(2-methyl-2H-tetrazol-5-yl)quinoline). The cationic complexes were readily adsorbed to negatively charged silica nanoparticles and trapped in the sol-gel matrix. The sol-to-solid phase transfer was performed by using an innovative spray-freeze-drying technique, leading to the formation of phosphorescent solid micro-granules. The structural and optical characterisation of the Ir(iii) complexes together with SiO2nanoparticles, nanosols (Ir@SiO2) and powders (Ir@SiO2powders), revealed how the presence of the Ir(iii)-based complexes did not alter the morphology of the colloidal silica or granulated phases. Moreover, the silica matrix did not interfere with the optical properties of the embedded complexes. The distribution of [F2IrPTZ-Me]+and [IrQTZ-Me]+in the spray-freeze-dried powders was qualitatively evaluated by fluorescence microscopy, revealing how the luminescent particles were homogeneously dispersed all over the silica matrix. Interestingly, in aqueous solution the release of complex [F2IrPTZ-Me]+from the corresponding Ir@SiO2powder is almost negligible, therefore suggesting that a strong interaction occurs between the host-silica matrix and the Ir(iii) guest complex