Bones And Base Pairs: A Look Inside American G.i.’s Missing in Action

Identification of remains of missing servicemen lost overseas is a very difficult task. Identification based on osteology supplemented by identification based on DNA analysis optimizes the chances of positively identifying a soldier. For this thesis project, I examined how both osteology and mitochondrial DNA (mtDNA) can be employed in identifications. To learn more about the way that DNA can be employed in identification cases, I worked on a project that studied the genetic ancestry of an indigenous Mexican population known as the Popoluca. The Popoluca are a Totonocan speaking people which are divided into four social groups spread across 25 towns and hamlets in Veracruz. The mtDNAs of Popoluca individuals were surveyed for sequence variation. The results of the study indicated that the Popoluca are comprised of individuals belonging to either haplogroups A or C, with the exception of one individual from haplogroup B. The Popoluca individuals also contained some haplotypes that were unique to them compared to other Native Mexican populations. Such uniqueness demonstrates how the comparison of mtDNA could prove useful in identification cases

Men Are from Quartile One, Women Are from? Relative Age Effect in European Soccer and the Influence of Age, Success, and Playing Status

The relative age effect (RAE) is characterised by an overrepresentation of athletes born earlier in the selection year. Whilst an RAE is consistently evident in male soccer, examinations in female players remain limited. The aim of the present study was to examine the influence of sex, as well as age, success, and playing status in European soccer players. The sample consisted of a total of 6546 soccer players from 55 soccer nations that competed in recent European Championship qualification campaigns. Results indicated an evident RAE in male [p = 0.017] but not female [p = 0.765] players. Male players were over-represented by players born in the first quartile for the U17 [p < 0.001] and U19 [p = 0.001] levels, however, this over-representation did not transfer to senior levels. No RAE was observed at any level for female players. Inside each age group, a slight selection bias towards those born in the first quartile for successful squads was observed but did not significantly differentiate between qualification status for either male or female players. Results from this study highlight the disparity in RAE prevalence between male and female players and raise further questions regarding the value of selecting relatively older players to metrics of success, transition, and selection for senior international soccer

Interdisciplinary doctoral research networks: enhancers and inhibitors of social capital development

Interdisciplinary research networks are increasing, with professionals encouraged to undertake research across disciplines to increase innovation, creativity and knowledge. More recently, this interdisciplinary focus is being mirrored by the establishment of interdisciplinary doctoral research networks. But do these networks work? And if so, how and why? We employ social capital theory to (a) understand the lived experiences of students in interdisciplinary doctoral programmes and (b) build programme design theory to support the development of social capital within such programmes. We present the results of 28 semi-structured interviews conducted with doctoral students from three European Union funded interdisciplinary research training networks to understand how they perceive the enhancers, inhibitors and manifestations of social capital within their networks. Key themes revolve around ‘extracting value from the interdisciplinary process’, ‘motivating students throughout the interdisciplinary programme journey’, and ‘relating to others both within and external to the programme’. We propose a framework for interdisciplinary programme design

Specific Adherence of Sporangia of a Paenibacillus Sp. Bacterium to Heterorhabditis Spp. Nematodes. Hitching a Ride to Lunch?

The fact that entomopathogenic nematodes of the genera Heterorhabditis and Stemernema are normally found in mutuahstic association with the bacteria Photorhabdus spp. and Xenorhabdus spp , respectively, has long been universally accepted However, the extent and nature of their interaction with bacteria other than these, under natural conditions, is less well known. There have been a number of reports of other bacteria being isolated from entomopathogenic nematodes, particularly from Stemernema spp. (reviewed by Boemare et al, 1998a). Jackson et al (1995) reported the occurrence oí Providencia rettgeri with a number of strains of Heterorhabditis spp. originating from different geographical regions. Boemare et al (1998b) point out that écologiste will tend to harvest nematodes resulting from "successful" parasitisms, i e. those where the cadaver is not more rapidly putrefied by the presence of co-associated bacteria other that the natural symbiont. With this in mind it is conceivable that we may under-estimate the frequency of association of these nematodes with other bacteria

Long-Term Safety Evaluation of Ubrogepant for the Acute Treatment of Migraine: Phase 3, Randomized, 52-Week Extension Trial.

OBJECTIVE: To evaluate the long-term safety and tolerability of ubrogepant for the acute treatment of migraine. BACKGROUND: Ubrogepant is an oral, calcitonin gene-related receptor antagonist in development for the acute treatment of migraine. The efficacy of ubrogepant was demonstrated in 2 phase 3 trials in which a significant improvement was observed in migraine headache pain, migraine-associated symptoms, and ability to function. METHODS: This was a phase 3, multicenter, randomized, open-label, 52-week extension trial. Adults with migraine with or without aura entered the trial after completing one of 2 phase 3 lead-in trials and were re-randomized 1:1:1 to usual care, ubrogepant 50 mg, or ubrogepant 100 mg. Randomization to ubrogepant dose was blinded. Those randomized to usual care continued to treat migraine attacks with their own medication. The usual care arm was included in this trial to capture background rates of hepatic laboratory parameters and contextualize hepatic safety assessments. Safety and tolerability were the primary outcome measures. The safety population for the ubrogepant arms included all randomized participants who received at least 1 dose of treatment. All cases of alanine aminotransferase (ALT)/aspartate aminotransferase (AST) elevations of ≥3 times the upper limit of normal were adjudicated by an independent panel of liver experts who were blinded to dose. RESULTS: The safety population included 1230 participants (404 in the ubrogepant 50-mg group, 409 in the ubrogepant 100-mg group, and 417 in the usual care group). Participants were on average 42 years of age, 90% (1106/1230) female and 85% (1043/1230) white, with an average BMI of 30 kg/m CONCLUSIONS: Long-term intermittent use of ubrogepant 50 and 100 mg given as 1 or 2 doses per attack for the acute treatment of migraine was safe and well tolerated, as indicated by a low incidence of treatment-related TEAEs and SAEs and discontinuations due to adverse events in this 1-year trial

Relative age effect across the talent identification process of youth female soccer players in the United States: influence of birth year, position, biological maturation, and skill level

The aims of the study were to examine the relative age effect (RAE) in youth female soccer players in the United States (US) and the influence of birth year, playing position, estimated maturation and skill ratings. The sample consisted of 3,364 youth female soccer players who were active in the 2021–2022 US soccer season across three main stages of the talent identification (TID) process for Youth National Team (YNT) players (i.e., Club, TID Center, and YNT). A prevalent RAE for players born in Q1 was present in the full sample. A significant prevalence for Q1 players were identified for both Club and TID Center, but not YNT. A significant RAE prevalence for Q1 players was identified for most of the age groups from U13–U18 at Club (except U18) and TID Center (except U17). Significant RAEs prevalence for players born in Q1 were found in Goalkeepers, Center Backs, Midfielders, and Center Forwards at Club and TID Center (except Wide Forwards). The data identified a consistent RAE prevalence for Q1 players in early and on-time-maturers across all levels. An even birthdate spread was evident in YNT with a prevalence for Q4 players and a higher percentage of late maturers than elsewhere in the TID process. Results reinforce evidence indicating RAEs still exist in soccer, yet show for the first time within a youth female soccer TID process, the influence of contextual factors on the prevalence of RAE. This information can be used to advance TID and development across the US soccer landscape

The Effects of Protein Kinase C Beta II Peptide Modulation on Superoxide Release in Rat Polymorphonuclear Leukocytes

Phorbol 12-myristate 13-acetate (PMA; a diacylglycerol mimetic) is known to augment polymorphonuclear leukocyte (PMN) superoxide (SO) release via protein kinase C (PKC) activation. However, the role of PKC beta II (βII) mediating this response is not known. It’s known that myristic acid (myr-) conjugation facilitates intracellular delivery of the cargo sequence, and that putative PKCβII activator and inhibitor peptides work by augmenting or attenuating PKCβII translocation to cell membrane substrates (e.g. NOX-2). Therefore, we hypothesize that myr- conjugated PKCβII peptide-activator (N-myr-SVEIWD; myr-PKCβ+) would increase PMA-induced rat PMN SO release, whereas, myr-PKCβII peptide-inhibitor (N-myr-SLNPEWNET; myr-PKCβ-) would attenuate this response compared to non-drug treated controls. Rat PMNs (5x106) were incubated for 15min at 370C in the presence/absence of myr-PKCβ+/- (20 μM) or SO dismutase (SOD;10μg/mL; n=8) as positive control. PMA (100nM) induced PMN SO release was measured spectrophotometrically at 550nm via reduction of ferricytochrome c for 390 sec. PMN SO release increased absorbance to 0.39±0.04 in non-drug treated controls (n=28), and 0.49±0.05 in myr-PKCβ+(n=16). This response was significantly increased from 180 seconds to 240 seconds (p\u3c0.05). By contrast, myr-PKCβ- (0.26±0.03; n=14) significantly attenuated PMA-induced SO release compared to non-drug controls and myr-PKCβ+ (p\u3c0.05). SOD-treated samples showed \u3e90% reduction of PMA-induced SO release and was significantly different from all groups (p\u3c0.01). Cell viability ranged between 94± to 98±2% in all groups as determined by 0.2% trypan blue exclusion. Preliminary results suggest that myr-PKCβ- significantly attenuates PMA-induced SO release, whereas myr-PKCβ+ significantly augments PMA-induced SO release, albeit transiently. Additional dose response and western blot experiments are planned with myr-PKCβ+/- in PMA-induced PMN SO release assays. This research was supported by the Department of Bio-Medical Sciences and the Division of Research at PCOM and by Young Therapeutics, LLC

Protein Kinase C Beta II Peptide Inhibitor Elicits Robust Effects on Attenuating Myocardial Ischemia/Reperfusion Injury

Reperfusion injury contributes to myocardial tissue damage following a heart attack partly due to the generation of reactive oxygen species (ROS) upon cardio-angioplasty. Protein kinase C beta II (PKCβII) inhibition during reperfusion with peptide inhibitor (N-myr-SLNPEWNET; PKCβII-) decreases ROS release and leukocyte infiltration in rat hind-limb and myocardial ischemia/reperfusion (I/R) studies, respectively. However, the role of activating PKCβII during reperfusion has not been previously determined. In this study, we hypothesize that myristoylated (myr)-PKCβII- will decrease infarct size and improve post-reperfused cardiac function compared to untreated controls, whereas PKCβII peptide activator (N-myr-SVEIWD; myr-PKCβII+) will show no improvement compared to control. Myristoylation of PKCβII peptides facilitate their entry into the cell in order to affect PKCβII activity by either augmenting or attenuating its translocation to cell membrane proteins, such as NOX-2. Isolated perfused rat hearts were subjected to global I(30min)/R(50min) and infused with myr-PKCβII+ (20μM; n=9), myr-PKCβII- (20µM; n=8), or plasma (control; n=9) at reperfusion. Hearts were frozen (-20oC), sectioned and stained using 1% triphenyltetrazolium chloride to differentiate necrotic tissue. The measurement of Left ventricular (LV) cardiac function was determined using a pressure transducer and infarct size was calculated as percent dead tissue vs. total heart tissue weight. Myr-PKCβII- significantly improved LV end-diastolic pressure 37±7 mmHg compared to control (58±5; p\u3c0.01) and myr-PKCβII+ (58±4; p\u3c0.01). Myr-PKCβII- significantly reduced infarct size to 14±3% compared to control (26±5%; p\u3c0.01), while myr-PKCβII+ (25±3%) showed no difference. The data indicate that myr-PKCβII- may be a putative treatment to reduce myocardial reperfusion injury when given to heart attack patients during cardio-angioplasty. Future studies are planned to determine infarct size by Image J analysis

Roadmaps to Utopia: Tales of the Smart City

Notions of the Smart City are pervasive in urban development discourses. Various frameworks for the development of smart cities, often conceptualized as roadmaps, make a number of implicit claims about how smart city projects proceed but the legitimacy of those claims is unclear. This paper begins to address this gap in knowledge. We explore the development of a smart transport application, MotionMap, in the context of a £16M smart city programme taking place in Milton Keynes, UK. We examine how the idealized smart city narrative was locally inflected, and discuss the differences between the narrative and the processes and outcomes observed in Milton Keynes. The research shows that the vision of data-driven efficiency outlined in the roadmaps is not universally compelling, and that different approaches to the sensing and optimization of urban flows have potential for empowering or disempowering different actors. Roadmaps tend to emphasize the importance of delivering quick practical results. However, the benefits observed in Milton Keynes did not come from quick technical fixes but from a smart city narrative that reinforced existing city branding, mobilizing a growing network of actors towards the development of a smart region. Further research is needed to investigate this and other smart city developments, the significance of different smart city narratives, and how power relationships are reinforced and constructed through them

Galaxy Zoo : 3D – crowdsourced bar, spiral, and foreground star masks for MaNGA target galaxies

Funding: Funding for the Sloan Digital Sky Survey IV has been provided by the Alfred P. Sloan Foundation, the U.S. Department of Energy Office of Science, and the Participating Institutions. We gratefully acknowledge the National Science Foundation’s support of the Keck Northeast Astronomy Consortium’s REU program through grants AST-1005024 and AST-1950797, the KINSC (Koshland Integrated Natural Sciences Centre) at Haverford College for Summer Scholar funding, and the Ogden Trust, UK for support for summer undergraduate internships.The challenge of consistent identification of internal structure in galaxies – in particular disc galaxy components like spiral arms, bars, and bulges – has hindered our ability to study the physical impact of such structure across large samples. In this paper we present Galaxy Zoo: 3D (GZ:3D) a crowdsourcing project built on the Zooniverse platform that we used to create spatial pixel (spaxel) maps that identify galaxy centres, foreground stars, galactic bars, and spiral arms for 29 831 galaxies that were potential targets of the MaNGA survey (Mapping Nearby Galaxies at Apache Point Observatory, part of the fourth phase of the Sloan Digital Sky Surveys or SDSS-IV), including nearly all of the 10 010 galaxies ultimately observed. Our crowdsourced visual identification of asymmetric internal structures provides valuable insight on the evolutionary role of non-axisymmetric processes that is otherwise lost when MaNGA data cubes are azimuthally averaged. We present the publicly available GZ:3D catalogue alongside validation tests and example use cases. These data may in the future provide a useful training set for automated identification of spiral arm features. As an illustration, we use the spiral masks in a sample of 825 galaxies to measure the enhancement of star formation spatially linked to spiral arms, which we measure to be a factor of three over the background disc, and how this enhancement increases with radius.Publisher PDFPeer reviewe