Effect of Monoethanolamine on the Lipid Metabolism of Prostate Cancer Cells

The ability of cancer cells to synthesize and store lipids in the form of lipid droplets (LDs) gives them a proliferative advantage over normal cells. Here, we elucidate the effect of monoethanolamine (Etn) on the lipid metabolism of LD-rich prostate carcinoma (PC3) cells. Etn is a ubiquitous molecule that serves as a phospholipid precursor and has been shown to have anticancer activity, although its mechanism of action is not fully understood. PC3 cells were treated with Etn and evaluated with a combination of biochemical and cell biology techniques. Treatment with Etn caused a decrease in cytosolic LD abundance induced by Ca2+-stimulated lipolysis. Our study effectively implicates the disruption of lipid metabolism as a contributor to Etn-induced cell death. This finding opens new avenues for the development of LD-targeted anticancer therapy

Intensive Pedestrain Survey of Three Tracts to be Impacted by the Planned Expansion of the San Antonio International Airport, Bexar County, Texas

During the months of March and April 2005, the Center for Archaeological Research (CAR) of The University of Texas at San Antonio conducted a 100 percent intensive pedestrian survey of three tracts of land (totaling 210 acres) for Adams Environmental, Inc. The project area is located within the boundaries of the San Antonio International Airport and the tracts are owned by the City of San Antonio Department of Aviation. The tracts represent the planned locations for the expansion of two runways and land development adjacent to them. The goal of the archaeological survey was to identify and document all prehistoric and/or historic archaeological sites in the project area. The archaeological survey revealed that there were major disturbances in all three tracts. No prehistoric and/or historic sites were encountered and no additional archaeological work is recommended in association with the runway expansion project. The possibility that an alternative Widely Spaced Runway Option would be pursued for airport expansion was also considered as part of this project. The area that would be impacted by this alternative option is located on the northdescending bank of Salado Creek. Additional archaeological work, in the form of a 100 percent intensive pedestrian survey coupled with a standing structure survey, is recommended within this alternative area if this is the final option chosen for development. All artifacts collected during these investigations are curated at the CAR facility according to Texas Historical Commission guidelines. These investigations were conducted under Texas Antiquities Permit No. 3619, with Dr. Steve Tomka, CAR director, serving as Principal Investigator

Archaeological Survey and Archival Research of the Naegelin Tract (41BX1600) in San Antonio, Bexar County, Texas

On December 2 and 3, 2004, the Center for Archaeological Research (CAR) conducted a Phase I survey of the 108-acre Naegelin Tract in northwest Bexar County, Texas, for Raba-Kistner Consultants, Inc. The proposed development consists of extending De Zavala Road through the southern portion of the tract and Kyle Seale Road through the eastern margin of the tract. Drainage easements are planned to cross through the center of the property and also along the northern and eastern margins of the tract. Thirty-six shovel tests were excavated along 30-meter transects and in areas considered to have high or moderate probability of buried cultural materials. No buried cultural materials were identified by any of the hand-excavated shovel tests. The survey resulted in the identification of eight isolated finds including one heavily fragmented projectile point that may represent a Bulverde dart point. This was the only artifact collected during the survey. No prehistoric sites were identified within the project area. One historic site was identified during the survey (41BX1600). The survey documented the remains of a main stone building and nine outbuildings on the property. Although an age could not be determined based on the sparse artifacts noted on surface, the site appeared to represent a historic homestead built during the late nineteenth or early twentieth centuries. A 1939 aerial photograph of the area shows that many of the outbuildings and the main stone structure had been built by this date. Comprehensive deed research and oral histories conducted between February and June, 2005, helped to establish that the earliest structures on the complex were built in 1888. This research also revealed the presence of an unmarked grave on the property. Subsequent to the pedestrian survey and prior to the completion of the archival research, the main stone building was severely damaged by fire. The fire damage exposed construction and architectural details not visible during the pedestrian survey. During a subsequent site visit, a tenth wood-framed outbuilding was identified. One isolated stone building of the same construction technique and age remained in good condition following the fire until it was recently torn down during asbestos abatement activities. The principal stone-built structures on the compound were exceptional examples of Texas vernacular architecture of the late nineteenth and early twentieth centuries. The interior of the main stone structure had been dilapidated through years of neglect and abandonment, nonetheless, it retained structural integrity and exemplified changes in architectural elements over a period of roughly 10 years of additions to the original core structure. Following the fire, and the razing of the only undamaged building on site, the stone buildings retain no structural integrity and the site is not recommended as eligible for nomination to the National Register of Historic Places or for designation as a State Archeological Landmark. No additional archaeological work is recommended in association with this development. These archaeological investigations were conducted under Texas Antiquities Committee permit number 3597 with Dr. Raymond P. Mauldin, CAR Assistant Director, serving as Principal Investigator. The single prehistoric artifact collected during the survey and a small number of potentially temporally diagnostic artifacts collected from the surface of 41BX1600 following the fire, as well as all project-related documents are curated at the Center for Archaeological Research at The University of Texas at San Antonio

A genome-wide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish

BACKGROUND: The genomic organisation of the Major Histocompatibility Complex (MHC) varies greatly between different vertebrates. In mammals, the classical MHC consists of a large number of linked genes (e.g. greater than 200 in humans) with predominantly immune function. In some birds, it consists of only a small number of linked MHC core genes (e.g. smaller than 20 in chickens) forming a minimal essential MHC and, in fish, the MHC consists of a so far unknown number of genes including non-linked MHC core genes. Here we report a survey of MHC genes and their paralogues in the zebrafish genome. RESULTS: Using sequence similarity searches against the zebrafish draft genome assembly (Zv4, September 2004), 149 putative MHC gene loci and their paralogues have been identified. Of these, 41 map to chromosome 19 while the remaining loci are spread across essentially all chromosomes. Despite the fragmentation, a set of MHC core genes involved in peptide transport, loading and presentation are still found in a single linkage group. CONCLUSION: The results extend the linkage information of MHC core genes on zebrafish chromosome 19 and show the distribution of the remaining MHC genes and their paralogues to be genome-wide. Although based on a draft genome assembly, this survey demonstrates an essentially fragmented MHC in zebrafish

This Month

This Month is a series of collages that depict police violence at civil rights and Black Lives Matter protests. By pairing events from 1964 to contemporary protests that occurred in the same month, the collection draws a connection between the past and present

Desarrollo del Sistema de Comisiones 3S Strategic Software Solutions

La solución aplicada surgió con la necesidad de implementar una herramienta que permita la administración y mantenimiento de las comisiones generadas por las ventas de la empresa, que sea de ayuda para el cumplimiento de metas y objetivos planteados por parte de los empleados de la misma. Entre los problemas resueltos tenemos: Control y seguimiento de las ventas de cada uno de los productos. Unificación y creación de reportes presentados en las reuniones de directorio. Control y seguimiento de las ventas realizadas por cada empleado. Entre los resultados obtenidos tenemos: Permitir liquidar de manera automática y fácil las comisiones de los empleados de la empresa, donde se apliquen los parámetros establecidos para el pago de comisiones según el segmento del mercado. Mejor control de las actividades de los empleados, sobre todo las que no son medibles por sus resultados directos. Se asegura a la empresa que no se supere el % de coste que haya sido asignado al departamento comercial.The applied solution came with the need to implement a tool that allows the administration and maintenance fees generated by sales of the company, which is helpful for meeting goals and objectives by employees thereof. Among the unresolved issues we are: Control and monitoring of the sales of each of the products. Unification and creation of reports presented at board meetings. Control and monitoring of the sales made by each employee. Among the results we are: Allow automatic liquidate and easily commissions of employees of the company, where the parameters established for the payment of fees by market segment apply. Better control of employee activities , especially those that are not measurable by their direct results. Ensure that the company than% of cost that has been assigned to the sales department is not exceeded

Desarrollo del Sistema de Comisiones 3S Strategic Software Solutions

La solución aplicada surgió con la necesidad de implementar una herramienta que permita la administración y mantenimiento de las comisiones generadas por las ventas de la empresa, que sea de ayuda para el cumplimiento de metas y objetivos planteados por parte de los empleados de la misma. Entre los problemas resueltos tenemos: *Control y seguimiento de las ventas de cada uno de los productos. *Unificación y creación de reportes presentados en las reuniones de directorio. *Control y seguimiento de las ventas realizadas por cada empleado. Entre los resultados obtenidos tenemos: *Permitir liquidar de manera automática y fácil las comisiones de los empleados de la empresa, donde se apliquen los parámetros establecidos para el pago de comisiones según el segmento del mercado. *Mejor control de las actividades de los empleados, sobre todo las que no son medibles por sus resultados directos. *Se asegura a la empresa que no se supere el % de coste que haya sido asignado al departamento comercial.The applied solution came with the need to implement a tool that allows the administration and maintenance fees generated by sales of the company, which is helpful for meeting goals and objectives by employees thereof. Among the unresolved issues we are: *Control and monitoring of the sales of each of the products. *Unification and creation of reports presented at board meetings. *Control and monitoring of the sales made by each employee. Among the results we are: *Allow automatic liquidate and easily commissions of employees of the company, where the parameters established for the payment of fees by market segment apply. *Better control of employee activities , especially those that are not measurable by their direct results. *Ensure that the company than of cost that has been assigned to the sales department is not exceed

Edad de diagnóstico vs tratamiento de la displasia del desarrollo de la cadera (DDC) en los pacientes pediátricos atendidos en el servicio de ortopedia de la Novaclínica S.A. de Quito durante el período de enero a diciembre de 2011

Problema: La DDC es una entidad clínica que indirectamente califica la actuación de los servicios de salud de una nación. A pesar de los programas sistemáticos de cribado, los recién nacidos con caderas luxadas siguen siendo diagnosticados tardíamente en la infancia, lo cual retrasa el tratamiento oportuno y empeora el pronóstico1, sobretodo a partir del sexto mes o en aquellos que no responden bien al tratamiento ortopédico puede ser necesaria la cirugía correctora2. Según varios estudios se demuestra una elevada proporción de casos de Displasia del Desarrollo de la Cadera diagnosticados después de los seis meses de edad (un 35% del total de casos) Objetivo: Relacionar la edad de diagnóstico con el tratamiento efectivo de la Displasia del Desarrollo de la Cadera en los pacientes pediátricos atendidos en el Servicio de Ortopedia de la Novaclínica S.A. de Quito durante el período de Enero a Diciembre de 2011. Métodos: Se trata de un estudio de corte transversal, retrospectivo que pretende establecer la relación entre la edad de diagnóstico (precoz, oportuno, tardío) y el tratamiento efectivo de la DDC con respecto a: tiempo (prolongado / no prolongado), tipo de tratamiento (conservador u ortésico / quirúrgico), número de tratamientos (ortésicos / intervenciones quirúrgicas) y probabilidad de intervención quirúrgica. Se realizó una revisión de 1 023 historias clínicas obteniendo una muestra de 306 pacientes con diagnóstico de Displasia del Desarrollo de la Cadera. Resultados: Se calcula una incidencia de DDC típica de 45 por 100 pacientes pediátricos atendidos en la consulta de Ortopedia. El diagnóstico tardío se observó en el 29.7% de los pacientes. La presencia de malformaciones congénitas es un factor de riesgo para diagnóstico tardío. Las pacientes femeninas primogénitas con presentación de nalgas tienen más probabilidad de diagnóstico precoz al igual que las pacientes femeninas primogénitas procedentes de sector rural. El sexo femenino sumado al diagnóstico oportuno es un factor protector para intervención quirúrgica. Así mismo, el sexo femenino sumado al diagnóstico tardío es un factor de riesgo para intervención quirúrgica. El diagnostico tardío incrementa en cuatro veces la probabilidad de tener un tratamiento prolongado y nueve veces la de ser intervenido quirúrgicamente. Conclusiones: La edad de diagnóstico está en relación directa con el tratamiento resolutivo de la DDC en los pacientes pediátricos atendidos en el Servicio de Ortopedia de la Novaclínica S.A. de Quito durante el período de Enero a Diciembre de 2011. La proporción de diagnóstico tardío de DDC en este estudio es elevada, correspondiendo a más de un cuarto de los pacientes. El éxito del tratamiento de la DDC se fundamenta en la edad de instauración del mismo, existiendo una relación directa entre el diagnóstico precoz y el tratamiento efectivo de la DDC

Report on the Archaeological Investigations Conducted Between 1992 and 1993 at the Alamo Sales Museum (41BX6) Bexar County, Texas

Lone Star Archaeological Services, under the direction of Alton K. Briggs, conducted archaeological investigations in the vicinity and under the Sales Museum preceding the Sales Museum expansion project. The work was carried out between July 1991 and April 1993 under Texas Antiquities Committee permit number 1033 with Briggs serving as the Principal Investigator. Following the completion of the fieldwork, Briggs submitted several draft reports in order to fulfill permit requirements. The Texas Historical Commission rejected the draft reports and the permit lapsed into default. In 2003, the Center for Archaeological Research acquired the collection of artifacts recovered during the project and a fraction of the notes generated by the project. This report summarizes the results of the analysis conducted on the collection and describes the excavation results as they can be reconstructed based on the information available to the CAR staff. All artifacts collected during this project and all project-associated documentation is permanently curated at the Center for Archaeological Research according to Texas Historical Commission guidelines

Salivary Cortisol Analysis in Collegiate Female Lacrosse Athletes

International Journal of Exercise Science 16(6): 242-251, 2023. Cortisol is a hormone that corresponds to physiological and emotional stress. The purpose of this study was to 1) evaluate the changes in cortisol in female Division I collegiate lacrosse players (n = 15) throughout the competitive season, and 2) evaluate the correlation between cortisol and athlete wellness and workload. Salivary cortisol samples were collected weekly in the morning throughout the entirety of the 2021 competitive season (12 weeks). Subjective athlete total wellness scores and sub-scores (muscle soreness, sleep quality, fatigue, and stress) were taken on the same days. Objective total weekly Athlete Load (AL, an amalgam workload metric) were tabulated from the previous training week. A significant effect of time was found on wellness (p \u3c 0.001) and AL (p \u3c 0.001) over the twelve weeks with weekly differences, such as weeks with more than one game, weeks with no games, weeks with students in quarantine (not competing), or weeks with academic stressors such as final exams. There were no weekly differences in cortisol (p = 0.058). Cortisol had negligible correlations with wellness (r = -0.010, p = 0.889) and AL (r = 0.083, p = 0.272) during the competitive season. These findings suggest that cortisol changed little for athletes throughout the season although training volume and wellness did. Thus, assessing acute responses of cortisol may prove to be more beneficial to evaluating athletes’ stress