8 research outputs found
Alternativas asociativas para el abordaje integral de la actividad ganadera de peque帽os productores en el dpto. Molinos, Valle Calchaqu铆, Salta
La presente experiencia desarrollada a partir del a帽o 2003 hasta la actualidad se encuentra ubicada en el Valle Calchaqu铆 Norte, Departamento de Molinos, Provincia de Salta.
Involucra a m谩s de 350 familias campesinas pobladores de la alta monta帽a.
Las mismas viven en condiciones de marginalidad y pobreza donde su base productiva y econ贸mica se caracteriza por la cr铆a extensiva de ganado bovino y ovino complementada con la agricultura.
El trabajo desarrollado en el presente art铆culo surge a partir de la intervenci贸n del equipo t茅cnico perteneciente a la Agencia de Extensi贸n Rural del INTA Seclant谩s articulado con el equipo de la ONG Red Valles de Altura quienes, a partir de la generaci贸n de espacios participativos junto a las comunidades campesinas, inician un proceso de desarrollo end贸geno.
En los mencionados espacios se utilizaron metodolog铆as que promovieron participaci贸n como Diagn贸sticos Rurales Participativos y desde los cuales se evidenci贸 la necesidad de un abordaje integral a problem谩ticas de producci贸n y comercializaci贸n del ganado mayor y menor.
En este proceso se fueron fortaleciendo las capacidades locales, se promovi贸 y ejercit贸 el trabajo organizativo de las comunidades en conjunto con el equipo t茅cnico por lo que se logr贸 la implementaci贸n de 14 botiquines sanitarios de sanidad animal autogestivos; construcci贸n de sistemas de mangas y b谩sculas; mejora de instalaciones ganaderas y corrales; introducci贸n de gen茅tica; conformaci贸n de la Comisi贸n Zonal Alto Valle Calchaqu铆, asumiendo la coordinaci贸n aut贸noma de la Campa帽a anual de Lucha contra la Fiebre Aftosa; organizaci贸n y ejecuci贸n durante 6 a帽os de la Feria Campesina y ; Muestra Ganadera del Departamento de Molinos; consolidaci贸n de una Cooperativa Agropecuaria que aborda la resoluci贸n de limitantes de comercializaci贸n de la producci贸n ganadera y la inserci贸n en un mercado formal que le permite mejorar la calidad de vida de las familias involucradas.Trabajo galardonado con el Premio Biog茅nesis Bag贸, versi贸n 2011Academia Nacional de Agronom铆a y Veterinari
Alternativas asociativas para el abordaje integral de la actividad ganadera de peque帽os productores en el dpto. Molinos, Valle Calchaqu铆, Salta
La presente experiencia desarrollada a partir del a帽o 2003 hasta la actualidad se encuentra ubicada en el Valle Calchaqu铆 Norte, Departamento de Molinos, Provincia de Salta.
Involucra a m谩s de 350 familias campesinas pobladores de la alta monta帽a.
Las mismas viven en condiciones de marginalidad y pobreza donde su base productiva y econ贸mica se caracteriza por la cr铆a extensiva de ganado bovino y ovino complementada con la agricultura.
El trabajo desarrollado en el presente art铆culo surge a partir de la intervenci贸n del equipo t茅cnico perteneciente a la Agencia de Extensi贸n Rural del INTA Seclant谩s articulado con el equipo de la ONG Red Valles de Altura quienes, a partir de la generaci贸n de espacios participativos junto a las comunidades campesinas, inician un proceso de desarrollo end贸geno.
En los mencionados espacios se utilizaron metodolog铆as que promovieron participaci贸n como Diagn贸sticos Rurales Participativos y desde los cuales se evidenci贸 la necesidad de un abordaje integral a problem谩ticas de producci贸n y comercializaci贸n del ganado mayor y menor.
En este proceso se fueron fortaleciendo las capacidades locales, se promovi贸 y ejercit贸 el trabajo organizativo de las comunidades en conjunto con el equipo t茅cnico por lo que se logr贸 la implementaci贸n de 14 botiquines sanitarios de sanidad animal autogestivos; construcci贸n de sistemas de mangas y b谩sculas; mejora de instalaciones ganaderas y corrales; introducci贸n de gen茅tica; conformaci贸n de la Comisi贸n Zonal Alto Valle Calchaqu铆, asumiendo la coordinaci贸n aut贸noma de la Campa帽a anual de Lucha contra la Fiebre Aftosa; organizaci贸n y ejecuci贸n durante 6 a帽os de la Feria Campesina y ; Muestra Ganadera del Departamento de Molinos; consolidaci贸n de una Cooperativa Agropecuaria que aborda la resoluci贸n de limitantes de comercializaci贸n de la producci贸n ganadera y la inserci贸n en un mercado formal que le permite mejorar la calidad de vida de las familias involucradas.Trabajo galardonado con el Premio Biog茅nesis Bag贸, versi贸n 2011Academia Nacional de Agronom铆a y Veterinari
Aplicaciones de herramientas de investigaci贸n acci贸n participativa para purificaci贸n y calentamiento de agua para uso sanitario en comunidades andinas aisladas de Salta
En este trabajo se aborda la problem谩tica de la adopci贸n y apropiaci贸n de tecnolog铆as para el aprovechamiento de la energ铆a solar en comunidades campesinas aisladas. Se describe una metodolog铆a de trabajo que aplica herramientas de Investigaci贸n Acci贸n Participativa a una experiencia que es llevada a cabo en dos comunidades rurales de Salta: Esquina de Guardia, donde las acciones se orientaron a resolver el problema de aguas arsenicales a trav茅s de destiladores solares; y Cabrer铆as, donde la comunidad demandaba agua caliente para uso sanitario. Un primer an谩lisis, nos lleva a concluir que no existe un procedimiento metodol贸gico 煤nico para abordar las experiencias de transferencia y apropiaci贸n tecnol贸gica, estando estos condicionados al contexto en que se realizan.The problem that is focus this work is the existing difficulty to realise the adoption and appropriation of technology, with the consequent failure of many projects and the lack of methodologies that favor the participation of recipients in the transfer. This paper describes a methodology that applies tools of Participatory Action Research, which supports a process of technology transfer and ongoing ownership. This experience is conducted in two rural communities of Salta: Esquina de Guardia, where actions were aimed at solving the problem of arsenic in water through solar stills, and Cabrer铆as, where the community, in a process of improving quality of life, demanding hot water for sanitary use. An initial analysis leads us to conclude that there is not single methodological approach to address the experiences of technology transfer and appropriation, being those conditioned to the context in which they perform.Asociaci贸n Argentina de Energ铆as Renovables y Medio Ambiente (ASADES
Aplicaciones de herramientas de investigaci贸n acci贸n participativa para purificaci贸n y calentamiento de agua para uso sanitario en comunidades andinas aisladas de Salta
En este trabajo se aborda la problem谩tica de la adopci贸n y apropiaci贸n de tecnolog铆as para el aprovechamiento de la energ铆a solar en comunidades campesinas aisladas. Se describe una metodolog铆a de trabajo que aplica herramientas de Investigaci贸n Acci贸n Participativa a una experiencia que es llevada a cabo en dos comunidades rurales de Salta: Esquina de Guardia, donde las acciones se orientaron a resolver el problema de aguas arsenicales a trav茅s de destiladores solares; y Cabrer铆as, donde la comunidad demandaba agua caliente para uso sanitario. Un primer an谩lisis, nos lleva a concluir que no existe un procedimiento metodol贸gico 煤nico para abordar las experiencias de transferencia y apropiaci贸n tecnol贸gica, estando estos condicionados al contexto en que se realizan.The problem that is focus this work is the existing difficulty to realise the adoption and appropriation of technology, with the consequent failure of many projects and the lack of methodologies that favor the participation of recipients in the transfer. This paper describes a methodology that applies tools of Participatory Action Research, which supports a process of technology transfer and ongoing ownership. This experience is conducted in two rural communities of Salta: Esquina de Guardia, where actions were aimed at solving the problem of arsenic in water through solar stills, and Cabrer铆as, where the community, in a process of improving quality of life, demanding hot water for sanitary use. An initial analysis leads us to conclude that there is not single methodological approach to address the experiences of technology transfer and appropriation, being those conditioned to the context in which they perform.Asociaci贸n Argentina de Energ铆as Renovables y Medio Ambiente (ASADES
Ultrastructural study of the TG180 murine sarcoma cell invasion by Toxoplasma gondii: comparison between in vivo and in vitro cell cultures
Infection of non-adherent TG180 murine sarcoma cells with Toxoplasma gondii was compared, at the ultrastructural level, in both in vivo and in vitro conditions. Suspensions of 3.0 x 10(6) TG180 cells infected in vitro with 1.0 x 10(6) parasites of the RH strain were harvested between the first and 6th day post-infection and processed for transmission electron microscopy. In vivo infection was made by intraperitoneal inoculation in mice of 1.0 x 10(6) TG180 cells, that were co-inoculated with a parasite suspension at the same cell concentration. Cells were harvested 10, 20, 30 min and 24, 48 h post-inoculation and processed for transmission electron microscopy at the same conditions of the in vitro culture. It was observed TG180 murine sarcoma cells with intense and equivalent intracellular parasitism in both conditions. Host cells with parasitophorous vacuoles containing up to 16 parasites, as well as parasites undergoing mitoses or presenting a bradyzoite-like morphology, were frequently seen in both culture methods
Macrophage Migration Inhibitory Factor (MIF) Prevents Maternal Death, but Contributes to Poor Fetal Outcome During Congenital Toxoplasmosis
Migration inhibitory factor (MIF) is a pro-inflammatory cytokine that plays important roles in physiology, pathology, immunology and parasitology, including the control of infection by protozoa parasites such as Toxoplasma gondii. As the MIF function in congenital toxoplasmosis is not fully elucidated yet, the present study brings new insights for T. gondii infection in the absence of MIF based on pregnant C57BL/6MIF-/- mouse models. Pregnant C57BL/6MIF-/- and C57BL/6WT mice were infected with 05 cysts of T. gondii (ME49 strain) on the first day of pregnancy (dop) and were euthanized at 8 dop. Non-pregnant and non-infected females were used as control. Our results demonstrated that MIF-/- mice have more accentuated change in body weight and succumbed to infection first than their WT counterparts. Otherwise, pregnancy outcome was less destructive in MIF-/- mice compared to WT ones, and the former had an increase in the mast cell recruitment and IDO expression and consequently presented less inflammatory cytokine production. Also, MIF receptor (CD74) was upregulated in MIF-/- mice, indicating that a compensatory mechanism may be required in this model of study. The global absence of MIF was associated with attenuation of pathology in congenital toxoplasmosis, but resulted in female death probably because of uncontrolled infection. Altogether, ours results demonstrated that part of the immune response that protects a pregnant female from T. gondii infection, favors fetal damage
A Transcript Finishing Initiative for Closing Gaps in the Human Transcriptome
We report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST clusters, mapped against the genomic sequence. Each pair of EST clusters selected for experimental validation was designated a transcript finishing unit (TFU). A total of 489 TFUs were selected for validation, and an overall efficiency of 43.1% was achieved. We generated a total of 59,975 bp of transcribed sequences organized into 432 exons, contributing to the definition of the structure of 211 human transcripts. The structure of several transcripts reported here was confirmed during the course of this project, through the generation of their corresponding full-length cDNA sequences. Nevertheless, for 21% of the validated TFUs, a full-length cDNA sequence is not yet available in public databases, and the structure of 69.2% of these TFUs was not correctly predicted by computer programs. The TF strategy provides a significant contribution to the definition of the complete catalog of human genes and transcripts, because it appears to be particularly useful for identification of low abundance transcripts expressed in a restricted set of tissues as well as for the delineation of gene boundaries and alternatively spliced isoforms