97 research outputs found
Micro-generador termoeléctrico basado en contactos eléctricos pasantes
Micro-generador termoeléctrico que comprende
porciones (P, N) de material semiconductor
alternadas entre sĂ, que definen filas (3) de material
semiconductor agrupadas formando al menos dos
capas horizontales (4) de material semiconductor,
estando dichas filas separadas verticalmente entre sĂ
por låminas (1) de sustrato eléctricamente aislante y
térmicamente conductor provistas de orificios
pasantes (5) que conectan eléctricamente las
porciones (P, N) de material semiconductor de una
capa (4), con las porciones (N, P) de material
semiconductor de la capa (4) inmediatamente
superior, creando columnas (8) verticales de
termopares (7)Peer reviewedUniversidad PolitĂ©cnica de Valencia, Centro de Transferencia de TecnologĂa, Consejo Superior de Investigaciones CientĂficasB1 Patente sin examen previ
Cova dâEn Pardo (Planes, Alicante). Un avance sobre la secuencia cultural
La valoraciĂłn general de las recientes excavaciones en la Cova dâEn Pardo (1993 â2006) permiten presentar la secuencia de ocupaciĂłn de la cavidad, en lo que la Prehistoria reciente se refiere, desde un NeolĂtico caracterizado por la presencia de cerĂĄmicas impresas hasta el Bronce Final. Se adelanta ahora la revisiĂłn de la sedimentologĂa, nuevas dataciones de radiocarbono y la valoraciĂłn cultural de los distintos niveles a partir de la valoraciĂłn de la cerĂĄmica localizada en las intervenciones de la denominada sala de la derecha.The valuation in general of the recent excavations at the En Pardoâs Cave (1993-2006) presents the sequence of the cavity occupation, as it recent Prehistory refers to, from a Neolithic which characterizes for the presence of printed ceramics to the Final Bronze. It implies to go further with the review of the sedimentology, new radiocarbon data and the cultural evaluation of the different levels obtained from the valuation of the ceramic found during the interventions done at the so-called gallery of the right
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PPAR<i>Îł</i> agonist-loaded PLGA -PEG nanocarriers as a potential treatment for Alzheimerâs disease: in vitro and in vivo studies
Objective: The first aim of this study was to develop a nanocarrier that could transport the peroxisome proliferator-activated receptor agonist, pioglitazone (PGZ) across brain endothelium and examine the mechanism of nanoparticle transcytosis. The second aim was to determine whether these nanocarriers could successfully treat a mouse model of Alzheimerâs disease (AD).
Methods: PGZ-loaded nanoparticles (PGZ-NPs) were synthesized by the solvent displacement technique, following a factorial design using poly (lactic-co-glycolic acid) polyethylene glycol (PLGA-PEG). The transport of the carriers was assessed in vitro, using a human brain endothelial cell line, cytotoxicity assays, fluorescence-tagged nanocarriers, fluorescence-activated cell sorting, confocal and transmission electron microscopy. The effectiveness of the treatment was assessed in APP/PS1 mice in a behavioral assay and by measuring the cortical deposition of ÎČ-amyloid.
Results: Incorporation of PGZ into the carriers promoted a 50x greater uptake into brain endothelium compared with the free drug and the carriers showed a delayed release profile of PGZ in vitro. In the doses used, the nanocarriers were not toxic for the endothelial cells, nor did they alter the permeability of the bloodâbrain barrier model. Electron microscopy indicated that the nanocarriers were transported from the apical to the basal surface of the endothelium by vesicular transcytosis. An efficacy test carried out in APP/PS1 transgenic mice showed a reduction of memory deficit in mice chronically treated with PGZ-NPs. Deposition of ÎČ-amyloid in the cerebral cortex, measured by immunohistochemistry and image analysis, was correspondingly reduced.
Conclusion: PLGA-PEG nanocarriers cross brain endothelium by transcytosis and can be loaded with a pharmaceutical agent to effectively treat a mouse model of AD
PPARÎł agonist-loaded PLGA-PEG nanocarriers as a potencial treatment for Alzheimer's disease: in vitro and in vivo studies.
Objective: The first aim of this study was to develop a nanocarrier that could transport the peroxisome proliferator-activated receptor agonist, pioglitazone (PGZ) across brain endothelium and examine the mechanism of nanoparticle transcytosis. The second aim was to determine whether these nanocarriers could successfully treat a mouse model of Alzheimer's disease (AD). Methods: PGZ-loaded nanoparticles (PGZ-NPs) were synthesized by the solvent displacement technique, following a factorial design using poly (lactic-co-glycolic acid) polyethylene glycol (PLGA-PEG). The transport of the carriers was assessed in vitro, using a human brain endothelial cell line, cytotoxicity assays, fluorescence-tagged nanocarriers, fluorescence-activated cell sorting, confocal and transmission electron microscopy. The effectiveness of the treatment was assessed in APP/PS1 mice in a behavioral assay and by measuring the cortical deposition of ÎČ-amyloid. Results: Incorporation of PGZ into the carriers promoted a 50x greater uptake into brain endothelium compared with the free drug and the carriers showed a delayed release profile of PGZ in vitro. In the doses used, the nanocarriers were not toxic for the endothelial cells, nor did they alter the permeability of the blood-brain barrier model. Electron microscopy indicated that the nanocarriers were transported from the apical to the basal surface of the endothelium by vesicular transcytosis. An efficacy test carried out in APP/PS1 transgenic mice showed a reduction of memory deficit in mice chronically treated with PGZ-NPs. Deposition of ÎČ-amyloid in the cerebral cortex, measured by immunohistochemistry and image analysis, was correspondingly reduced. Conclusion: PLGA-PEG nanocarriers cross brain endothelium by transcytosis and can be loaded with a pharmaceutical agent to effectively treat a mouse model of AD
Study of Regulatory T-Cells in Patients with Gastric Malt Lymphoma: Influence on Treatment Response and Outcome
Purpose
FOXP3+ regulatory T cells (Treg) play an essential role in modulating host responses to tumors and infections. The role of these cells in the pathogenesis of MALT lymphomas remains unknown. The aims of the study were to quantify the number of infiltrating FOXP3+ and CD3+ cells in patients with gastric MALT lymphoma at diagnosis and to study kinetics of these cells and CD20+ tumor cells after treatment and during long-term follow-up.
Methods
FOXP3+, CD3+ and CD20+ cells were analyzed by immunohistochemistry and the number of cells was quantified using a micrometric ocular. Samples of 35 patients with gastric MALT lymphoma at diagnosis and after treatment were included. Diagnostic samples were compared to 19 cases of chronic gastritis and diffuse large B-cell lymphoma (DLBCL) of the stomach.
Results
The median number of FOXP3+ infiltrating cells was higher (27 cells/cm2) in gastric MALT patients than in DLBCL (10 cells; pâ=â0.162) but similar to chronic gastritis (20 cells; pâ=â0.605). No characteristic or specific distribution pattern of infiltrating FOXP3+ cells was found. Gastric MALT lymphoma patients responding to bacterial eradication therapy had higher number of FOXP3+ cells at study entry. Kinetics of both infiltrating FOXP3+ cells and tumor CD20+ cells were strongly dependent on the treatment administered.
Discussion
Gastric MALT lymphomas have a number of Treg cells more similar to chronic gastritis than to DLBCL. Patients with higher number of tumor infiltrating FOXP3+ cells at study entry seem to have better response to antibiotics. Kinetics of Treg and tumor cells are influenced by type of treatmen
PIMEs i finançament: necessitats i alternatives
Postprint (published version
Experiencia inicial unicĂ©ntrica de la duodenoduodenostomĂa para el drenaje exocrino en el trasplante de pĂĄncreas
Trabajo presentado en el 17Âș Congreso de la Societat Catalana de Transplantament, celebrado en Barcelona, del 22 al 24 de marzo de 202
Understanding the first Neolithic occupation of Cova dâEn Pardo (Planes, Alicante): preliminary results of the multidisciplinary analysis of levels VIII and VIIIb
Se presentan los resultados de las excavaciones llevadas a cabo en la Cova dâEn Pardo (Planes, Alicante), concretamente los niveles VIII y VIIIb. El desarrollo de un proyecto multidisciplinar ha permitido caracterizar la ocupaciĂłn de una pequeña cavidad por parte de las primeras comunidades campesinas asociadas al inicio del proceso de neolitizaciĂłn del levante de la penĂnsula IbĂ©rica.We present the results of excavations carried out in the Cova dâEn Pardo (Planes, Alicante), specifically the levels VIII and VIIIb. The development of a multidisciplinary project has allowed characterize the occupation of a small cavity by the first farming communities associated with the Neolithization process of the Levant of Iberian Peninsula.Este trabajo se ha realizado en el marco del proyecto Origins and Spread of Agriculture in the western Mediterranean regiĂłn (ERC-2008-AdG 230561)
Association of Kv1.5 and Kv1.3 contributes to the major voltage-dependent K+ channel in macrophages
Voltage-dependent K(+) (Kv) currents in macrophages are mainly mediated by Kv1.3, but biophysical properties indicate that the channel composition could be different from that of T-lymphocytes. K(+) currents in mouse bone marrow-derived and Raw-264.7 macrophages are sensitive to Kv1.3 blockers, but unlike T-cells, macrophages express Kv1.5. Because Shaker subunits (Kv1) may form heterotetrameric complexes, we investigated whether Kv1.5 has a function in Kv currents in macrophages. Kv1.3 and Kv1.5 co-localize at the membrane, and half-activation voltages and pharmacology indicate that K(+) currents may be accounted for by various Kv complexes in macrophages. Co-expression of Kv1.3 and Kv1.5 in human embryonic kidney 293 cells showed that the presence of Kv1.5 leads to a positive shift in K(+) current half-activation voltages and that, like Kv1.3, Kv1.3/Kv1.5 heteromers are sensitive to r-margatoxin. In addition, both proteins co-immunoprecipitate and co-localize. Fluorescence resonance energy transfer studies further demonstrated that Kv1.5 and Kv1.3 form heterotetramers. Electrophysiological and pharmacological studies of different ratios of Kv1.3 and Kv1.5 co-expressed in Xenopus oocytes suggest that various hybrids might be responsible for K(+) currents in macrophages. Tumor necrosis factor-alpha-induced activation of macrophages increased Kv1.3 with no changes in Kv.1.5, which is consistent with a hyperpolarized shift in half-activation voltage and a lower IC(50) for margatoxin. Taken together, our results demonstrate that Kv1.5 co-associates with Kv1.3, generating functional heterotetramers in macrophages. Changes in the oligomeric composition of functional Kv channels would give rise to different biophysical and pharmacological properties, which could determine specific cellular responses
Strategies for annotation and curation of translational databases: the eTUMOUR project
The eTUMOUR (eT) multi-centre project gathered in vivo and ex vivo magnetic resonance (MR) data, as well as transcriptomic and clinical information from brain tumour patients, with the purpose of improving the diagnostic and prognostic evaluation of future patients. In order to carry this out, among other work, a databaseâthe eTDBâwas developed. In addition to complex permission rules and software and management quality control (QC), it was necessary to develop anonymization, processing and data visualization tools for the data uploaded. It was also necessary to develop sophisticated curation strategies that involved on one hand, dedicated fields for QC-generated meta-data and specialized queries and global permissions for senior curators and on the other, to establish a set of metrics to quantify its contents. The indispensable dataset (ID), completeness and pairedness indices were set. The database contains 1317 cases created as a result of the eT project and 304 from a previous project, INTERPRET. The number of cases fulfilling the ID was 656. Completeness and pairedness were heterogeneous, depending on the data type involved
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