The relevance of caseous lymphadenitis as a cause of culling in adult sheep

Four hundred and ninety-eight culled sheep received at the Ruminant Clinical Service of the Veterinary Faculty of Zaragoza, Spain, were examined in life and after humanitarian sacrifice in order to reach the final diagnosis of the cause of culling and to evaluate the presence of caseous lymphadenitis (CLA) lesions. One hundred and forty-seven of the 498 studied animals (29.52%) showed CLA compatible lesions that were subsequently confirmed by Corynebacterium pseudotuberculosis isolation. One hundred and seven of the 147 CLA affected animals presenting the visceral clinical form of the disease (72.79%), while only 32 animals were affected by the superficial form (21.77%). In addition, eight animals were found to be affected in both the visceral and the superficial presentations (5.44%). Eighty-four of the 147 CLA-affected animals (57.14%) did not show any concurrent disease, considering, in this case, CLA the main cause of culling (84/498: 16.87%). In the superficial presentation, the retropharyngeal lymph node, as a sole lesion, was the most frequently affected (13/32: 40.63%). Further, in the visceral form of the disease, 85.06% of the affected animals had the lesions located in the respiratory system (91/107: 85.06%). CLA was revealed as an important cause of culling in sheep production

Variable Shifts in the Autumn Migration Phenology of Soaring Birds in Southern Spain

While alteration of the migratory habits of birds is widely regarded as one of the most evident ecological effects of climate change, studies reporting shifts in migration phenology for long-lived, long-distance migrants have been few. Using time series of count data collected in southern Spain during autumn migration, we examined the magnitude and direction of phenological shifts for six common species of soaring birds. Many current methods for investigating phenological change rely on continuous data sets; however, these data may be unavailable for a variety of reasons. We used a cross-correlation analysis, which allowed us to compare recent data on the timing of migration from 1999–2011 to a historic data set collected during 1976–1977. The direction of phenological shifts for autumn migration was species-specific. White Storks Ciconia ciconia and Black Kites Milvus migrans appeared to have delayed passage, Black Storks Ciconia nigra and European Honey Buzzards Pernis apivorus have advanced their migratory timing, and we found no clear phenological change for Short-toed Eagles Circaetus gallicus or Booted Eagles Hieraaetus pennatus

Plasma chemistry and hematology reference values in wild nestlings of White-tailed Sea Eagles (Haliaeetus albicilla): effects of age, sex and hatching date

Studies on normal concentration of blood constituents of free-living birds are not very common. An adequate knowledge of blood chemistry is greatly recommended for those projects involving research and management of populations as far as they can be valuable for the assessment of the nutritional levels and health status of species. No previous published reports on these parameters from this species are available. The aim of this study was to obtain representative reference measures for hematologic and biochemical values in free-living clinically healthy wild White-tailed Sea Eagle nestlings (Haliaeetus albicilla). In addition, we investigated potential relationships between blood parameters, sex, age and hatching dates. Blood samples were obtained as part of routine monitoring and management when wild chicks were removed from their nest as part of a reintroduction program prerelease health check. A total of 83 nestlings, 43 males and 40 females, between 41 and 66 days of age (mean = 54.22, SD = 5.7), were sampled. Significant differences between sexes were found. Among hematological parameters, MCH, lymphocytes P and thrombocytes showed significant differences between males and females. In biochemical parameters, significant differences were found only in calcium, CK and LDH between sexes. No effect was found of age of the nestling when the sample was taken in any of the analyzed hematological parameters. No other significant relationships were found between biochemical parameters and other considered explanatory variables. Hatching date showed no relationship with blood parameters excepting urea. Urea was the only variable showing a strong relationship with hatching date, with those nestlings hatching later in the season showing higher urea concentration

Hacking Techniques Improve Health and Nutritional Status of Nestling White-tailed Eagles

Abstract Birds of prey frequently feature in reintroductions and the hacking technique is typically used. Hacking involves removing large nestlings from donor populations, transferring them to captivity, feeding them ad libitum. Potentially, via the hacking method, the stress of captivity and disruption of parental feeding may be detrimental. Alternatively, the provision of ad libitum food may be advantageous. Although hacking has underpinned reintroduction project successes there has been no research on how the method may affect the health and nutritional status of translocated birds during captivity. We compared blood chemistry data from 55 young White‐tailed Eagles, translocated from Norway as part of the species' reintroduction to Scotland, from sampling soon after arriving in captivity and again (≈42 days later) before their release. Numerous significant differences between the first and second samples were found, but no significant interactions showed that the sexes responded similarly to captivity. According to hematological and biochemical metrics, individuals showed several changes during captivity, including in red blood cell parameters, plasma proteins, and white cellular parameters related to the immune system, that indicated improved health status. Captivity with ad libitum food was associated with decreased urea and uric acid values: high values can indicate nutritional stress. Urea values became more normally distributed before release, indicating that ad libitum food had reduced nutritional differences between early nestlings in the season and later ones. Despite plentiful food, both sexes lost body mass before release, suggesting an inherent physiological mechanism to improve flight performance in fledglings. We conclude that hacking improved the health and nutritional status of released eagles which is likely to enable birds to cope with greater costs of exploratory behavior which they may require in reintroduction projects. In this context, we note the absence of survival differences between hacked and wild raptors in previous research

Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster.

Sequence variants in cis-acting enhancers are important for polygenic disease, but their role in Mendelian disease is poorly understood. Redundancy between enhancers that regulate the same gene is thought to mitigate the pathogenic impact of enhancer mutations. Recent findings, however, have shown that loss-of-function mutations in a single enhancer near PTF1A cause pancreas agenesis and neonatal diabetes. Using mouse and human genetic models, we show that this enhancer activates an entire PTF1A enhancer cluster in early pancreatic multipotent progenitors. This leading role, therefore, precludes functional redundancy. We further demonstrate that transient expression of PTF1A in multipotent progenitors sets in motion an epigenetic cascade that is required for duct and endocrine differentiation. These findings shed insights into the genome regulatory mechanisms that drive pancreas differentiation. Furthermore, they reveal an enhancer that acts as a regulatory master key and is thus vulnerable to pathogenic loss-of-function mutations

Improving speech intelligibility in hearing aids. Part I: Signal processing algorithms

[EN] The improvement of speech intelligibility in hearing aids is a traditional problem that still remains open and unsolved. Modern devices may include signal processing algorithms to improve intelligibility: automatic gain control, automatic environmental classification or speech enhancement. However, the design of such algorithms is strongly restricted by some engineering constraints caused by the reduced dimensions of hearing aid devices. In this paper, we discuss the application of state-of-theart signal processing algorithms to improve speech intelligibility in digital hearing aids, with particular emphasis on speech enhancement algorithms. Different alternatives for both monaural and binaural speech enhancement have been considered, arguing whether they are suitable to be implemented in a commercial hearing aid or not.This work has been funded by the Spanish Ministry of Science and Innovation, under project TEC2012-38142-C04-02.Ayllón, D.; Gil Pita, R.; Rosa Zurera, M.; Padilla, L.; Piñero Sipán, MG.; Diego Antón, MD.; Ferrer Contreras, M.... (2014). Improving speech intelligibility in hearing aids. Part I: Signal processing algorithms. Waves. 6:61-71. http://hdl.handle.net/10251/57901S6171

Improving speech intelligibility in hearing aids. Part II: Quality assessment

[EN] Subjective tests are the most reliable methods for quantifying the perceived speech intelligibility, but the process to perform these tests usually is time consuming and cost expensive. For this reason, different objective measures have been proposed in the literature to evaluate the intelligibility and/or quality of speech in such a way that cooperation of human listeners is not necessary. In this paper, we describe a wide range of subjective tests reported in the literature, focusing on those proposed to evaluate speech intelligibility of Spanish language, not only for normal hearing listeners, but for hearing impaired as well. Afterwards we summarize the most common objective measures of speech quality, and finally we perform a comparison between them and some subjective speech intelligibility tests. In the subjective tests, clean Spanish speech material has been contaminated with different real background noises: cafeteria and outside traffic noise. Results show that Short-Time Objective Intelligibility (STOI) and Perceptual Evaluation of Speech Quality (PESQ) indices present a better correlation and a lower mean square error when predicting intelligibility compared to other objective measures tested.This work has been supported by European Union ERDF and Spanish Government through TEC2012-38142-C04 project, and Generalitat Valenciana through PROMETEOII/2014/003 project. Participation of author A. Padilla has been supported by a postdoctoral fellowship from Conacyt (Mexico). The authors wish to acknowledge Prof. Felipe Orduña for his insightful comments that contributed to improve the manuscript, and to everyone who participated in the listening tests.Padilla, A.; Piñero Sipán, MG.; Diego Antón, MD.; Ferrer Contreras, M.; González Téllez, A.; Ayllón, D.; Gil Pita, R.... (2014). Improving speech intelligibility in hearing aids. Part II: Quality assessment. Waves. 6:73-85. http://hdl.handle.net/10251/57900S7385

Ecological specialization to fluctuating resources prevents long-distance migratory raptors from becoming sedentary on islands.

Background The adaptive transition between behavioral strategies, such as the shift from migratoriness to sedentariness, remains an outstanding question in evolutionary ecology. Density-dependent variation in the age of first breeding has been proposed as a feasible mechanism through which long-lived migratory birds with deferred sexual maturity should become sedentary to persist on islands. Although this pattern seems to hold for most raptors and herons, a few exceptions have been identified. One of these exceptions is the Eleonora's falcon, a long-distance migratory bird, which shows one of the most peculiar adaptations in the timing of reproduction and food requirements among raptors. Methodology/Principal Findings Here, we compiled data concerning demography, banding recoveries and satellite tracking of Eleonora's falcons to discuss likely explanations for the exceptional behavior of this insular long-distance migratory species. Conclusions/Significance New data reveal that Eleonora's falcons do return to the natal colonies in their first year and young birds are able to breed. However, in contrast to previous hypothesis, the highly specialized strategy of this and other ecologically similar species, as well as the virtual lack of food during winter at breeding areas prevent them from becoming sedentary on islands. Although the ultimate mechanisms underlying the process of sedentarization remain poorly understood, the evidence provided reveal the existence of important trade-offs associated with ecological specialization that may become particularly relevant in the present context of global change

A Genome-Wide siRNA Screen to Identify Modulators of Insulin Sensitivity and Gluconeogenesis

BACKGROUND: Hepatic insulin resistance impairs insulin's ability to suppress hepatic glucose production (HGP) and contributes to the development of type 2 diabetes (T2D). Although the interests to discover novel genes that modulate insulin sensitivity and HGP are high, it remains challenging to have a human cell based system to identify novel genes. METHODOLOGY/PRINCIPAL FINDINGS: To identify genes that modulate hepatic insulin signaling and HGP, we generated a human cell line stably expressing beta-lactamase under the control of the human glucose-6-phosphatase (G6PC) promoter (AH-G6PC cells). Both beta-lactamase activity and endogenous G6PC mRNA were increased in AH-G6PC cells by a combination of dexamethasone and pCPT-cAMP, and reduced by insulin. A 4-gene High-Throughput-Genomics assay was developed to concomitantly measure G6PC and pyruvate-dehydrogenase-kinase-4 (PDK4) mRNA levels. Using this assay, we screened an siRNA library containing pooled siRNA targeting 6650 druggable genes and identified 614 hits that lowered G6PC expression without increasing PDK4 mRNA levels. Pathway analysis indicated that siRNA-mediated knockdown (KD) of genes known to positively or negatively affect insulin signaling increased or decreased G6PC mRNA expression, respectively, thus validating our screening platform. A subset of 270 primary screen hits was selected and 149 hits were confirmed by target gene KD by pooled siRNA and 7 single siRNA for each gene to reduce G6PC expression in 4-gene HTG assay. Subsequently, pooled siRNA KD of 113 genes decreased PEPCK and/or PGC1alpha mRNA expression thereby demonstrating their role in regulating key gluconeogenic genes in addition to G6PC. Last, KD of 61 of the above 113 genes potentiated insulin-stimulated Akt phosphorylation, suggesting that they suppress gluconeogenic gene by enhancing insulin signaling. CONCLUSIONS/SIGNIFICANCE: These results support the proposition that the proteins encoded by the genes identified in our cell-based druggable genome siRNA screen hold the potential to serve as novel pharmacological targets for the treatment of T2D

Clogging transition of many-particle systems flowing through bottlenecks

When a large set of discrete bodies passes through a bottleneck, the flow may become intermittent due to the development of clogs that obstruct the constriction. Clogging is observed, for instance, in colloidal suspensions, granular materials and crowd swarming, where consequences may be dramatic. Despite its ubiquity, a general framework embracing research in such a wide variety of scenarios is still lacking. We show that in systems of very different nature and scale -including sheep herds, pedestrian crowds, assemblies of grains, and colloids- the probability distribution of time lapses between the passages of consecutive bodies exhibits a power-law tail with an exponent that depends on the system condition. Consequently, we identify the transition to clogging in terms of the divergence of the average time lapse. Such a unified description allows us to put forward a qualitative clogging state diagram whose most conspicuous feature is the presence of a length scale qualitatively related to the presence of a finite size orifice. This approach helps to understand paradoxical phenomena, such as the faster-is-slower effect predicted for pedestrians evacuating a room and might become a starting point for researchers working in a wide variety of situations where clogging represents a hindrance