Influence of Temperature and Copper on Oxalobacteraceae in Soil Enrichments

7 páginas.-- 3 figuras.-- 3 tablas.-- 30 referenciasβ-Proteobacteria is one of the most abundant phylum in soils, including autotrophic and heterotrophic ammonium-consumers with relevance in N circulation in soils. The effects of high-temperature events and phytosanitary treatments, such as copper amendments, on soil bacterial communities relevant to N-cycling remain to be studied. As an example, South Portugal soils are seasonally exposed to high-temperature periods, the temperature at the upper soil layers can reach over 40 °C. Here, we evaluated the dynamics of mesophilic and thermophilic bacteria from a temperate soil, in particular of heterotrophic β-Proteobacteria, regarding the ammonium equilibrium, as a function of temperature and copper treatment. Soil samples were collected from an olive orchard in southern Portugal. Selective enrichments were performed from samples under different conditions of temperature (30 and 50 °C) and copper supplementation (100 and 500 µM) in order to mime seasonal variations and phytosanitary treatments. Changes in the microbial communities under these conditions were examined by denaturing gradient gel electrophoresis, a molecular fingerprint technique. At moderate temperature—30 °C—either without or with copper addition, dominant members were identified as different strains belonging to genus Massilia, a genus of the Oxalobacteraceae (β-Proteobacteria), while at 50 °C, members of the Brevibacillus genus, phylum Firmicutes were also represented. Ammonium production during bacterial growth at moderate and high temperatures was not affected by copper addition. Results indicate that both copper and temperature selected specific tolerant bacterial strains with consequences for N-cycling in copper-treated orchards.This work was funded by FEDER Funds through the Operational Programme for Competitiveness Factors COMPETE and National Funds through FCT Foundation for Science and Technology under the Strategic Project PEst-C/AGR/UI0115/2011. We thank Eng. G. Pinheiro, from Eugénio Almeida Foundation (FEA) for providing access to the FEA olive orchard.Peer reviewe

Evolución de los deltas submarinos de los ríos Guadalfeo y Adra en respuesta a las variaciones de los aportes sedimentarios

The Guadalfeo and the Adra submarine deltas off the northern coast of the Alboran Sea have been built up under the direct influence of short and mountainous rivers. The area is subjected to strong climatic seasonality, with sporadic winter torrential floods and high summer aridity. In addition numerous anthropogenic activities have affected these systems, mostly during the last two centuries. In order to decode the influence of climatic variability and anthropogenic impacts on sediment supplies during the recent past, five sediment cores were collected from the Guadalfeo and Adra submarine deltas. Benthic foraminiferal and sedimentological analyses, combined with radiocarbon dating, were performed. The impact of torrential floods alternating with periods of low rainfall or dry periods were recorded in the Adra and Guadalfeo prodeltas. Periods with low abundance of benthic foraminifera and high amounts of coarse-grained sediments, were interpreted as the result of enhanced sediment supply to the shelf triggered by major flood events. On the other hand, periods with high amounts of fine-grained sediments and high abundances of colonizers and opportunistic foraminiferal species indicate the establishment of new environments with distinct ecological constraints. These environments were driven by lower sediment supplies during low rainfall or dry periods. The most recent sedimentation seems to reflect the human interventions in the rivers basins, such as deviation of the main river courses and dams construction, which reduced the sediment input and promoted the deposition of shallow-water submarine deltas.Versión del edito

The evolution of the Guadalfeo submarine delta (northern Alboran Sea) during the last ca. 200 years

The Guadalfeo submarine delta is located on the northern Alboran Sea shelf in the western Mediterranean Sea. The sedimentary dynamics of the deltaic system is governed by the discharge of one of the major rivers in this area draining the western sector of the near-coastal Sierra Nevada Mountains. The area is under the influence of a Mediterranean climate, with high spatial and temporal (i.e., inter- and intra-annual) rain variability. Major anthropogenic forcing affected the river system during the 1930’s, with the deviation of the main river channel 2.5 km to the west, to its present position. More recently, the construction of Béznar (1977-1985) and Rules (1993-2003) dams have also contributed to limit the amount of sediments exported to the deltaic system. In order to understand the interaction between river discharges and the evolution of the submarine delta at different timescales, sediment cores were collected off the ancient (core 13) and present-day (cores12 and 15) river courses. A chronological framework was performed and combined with sedimentological and benthic foraminiferal analyses. Radiocarbon dating of plant debris from the base of the cores indicates that the sedimentary record goes back 200 years. In core 13, the variations between coarse and fine fractions along the core and the upward increase of benthic foraminiferal population density, would indicate that deposition possibly occurred until the deviation of the main river course to its present position. In the lower part of core 12, the strong alternation between coarse and fine sediment textures and the variable amounts of benthic foraminiferal species are interpreted as the result of an active fluvial regime. The upper part, with high percentages of fine sediments and high values of population density, could be attributed to the stabilization of the river course in its present-day location. Core 15, located at 11 m water depth, showed the highest content of gravel in the lower part of the core, high contents of silt at two core depths and increased percentages of sand to the top, indicating the strong influence of human interventions in the river basin and consequent changes in the sediment supply to the Guadalfeo submarine delta.info:eu-repo/semantics/publishedVersio

Multi-proxy evidence of rainfall variability recorded in subaqueous deltaic deposits off the Adra River, southeast Iberian Peninsula

The Adra River deltaic system, southeast Iberian Peninsula, shows a steep topography and is subjected to strong climatic seasonality. This system has been affected by alternating wet and dry periods, and it has also undergone numerous anthropogenic activities such as deforestation, mining activities, river channel deviations and dam construction, particularly during the last two centuries. Two sediment cores were retrieved off the Adra River, from the western (MS_V9) and eastern (MS_V4) lobes of the subaqueous deltaic deposit. A multi-proxy study was carried out, including grain size, benthic foraminiferal assemblages, magnetic susceptibility and geochemical element analyses, in order to understand the sedimentary expression of recent climatic cycles and anthropogenic interventions in the river basin. Periods of increased deposition of coarse-grained sediments, low absolute abundance of benthic foraminifera and high elemental ratios indicative of terrigenous contributions, were interpreted as periods of increased sediment supply to the shelf. Four flooding events were recorded in core MS_V9 and three events on core MS_V4, of which two were observed in both cores. They were related to periods with major floods that were documented on the southern Iberian Peninsula around 1770e1810 and 1860 e1870 AD. On the other hand, sediment core intervals exhibiting increasing proportion of fine-grained sediments and higher abundances of foraminiferal species assigned as successful colonizers (Textularia earlandi) and opportunistic species that feed on bacteria or terrestrial organic matter (Bolivina ordinaria, Bulimina elongata, Eggerelloides scaber and Ammonia beccarii or tepida), indicate the establishment of new environments with new ecological constraints. They were related to significant decreases of terrigenous sediment input during low rainfall or dry periods. The increase of opportunistic species feeding on fresh phytodetritus (Nonionella iridea, Nonionella stella, Nonionella sp., Brizalina dilatata, Epistominella vitrea and Bolivinellina pseudopuntata), under more stable environmental conditions, also point to a stronger marine influence on the prodeltaic environments during these periods. The human interventions on the river basin after 1872 AD, with the deviation of the main river channel to the east, led to a drastic reduction of the sediment exported to the western delta lobe. This study showed that the sedimentation on the Adra subaqueous deltaic deposit was mainly controlled by rainfall variability from 1663 to 1872 AD, and afterwards by anthropogenic interventions.En prensa2,247

Optimal opportunistic screening of atrial fibrillation using pulse palpation in cardiology outpatient clinics: Who and how

Atrial fibrillation (AF) remain a prevalent undiagnosed condition frequently encountered in primary care.We aimed to find the parameters that optimize the diagnostic accuracy of pulse palpation to detect AF. We also aimed to create a simple algorithm for selecting which individuals would benefit from pulse palpation and, if positive, receive an ECG to detect AF.Nurses from four Cardiology outpatient clinics palpated 7,844 pulses according to a randomized list of arterial territories and durations of measure and immediately followed by a 12-lead ECG, which we used as the reference standard. We calculated the sensitivity and specificity of the palpation parameters. We also assessed whether diagnostic accuracy depended on the nurse's experience or on a list of clinical factors of the patients. With this information, we estimated the positive predictive values and false omission rates according to very few clinical factors readily available in primary care (age, sex, and diagnosis of heart failure) and used them to create the algorithm.The parameters associated with the highest diagnostic accuracy were palpation of the radial artery and classifying as irregular those palpations in which the nurse was uncertain about pulse regularity or unable to palpate pulse (sensitivity = 79%; specificity = 86%). Specificity decreased with age. Neither the nurse's experience nor any investigated clinical factor influenced diagnostic accuracy. We provide the algorithm to select the ≥40 years old individuals that would benefit from a pulse palpation screening: a) do nothing in <60 years old individuals without heart failure; b) do ECG in ≥70 years old individuals with heart failure; c) do radial pulse palpation in the remaining individuals and do ECG if the pulse is irregular or you are uncertain about its regularity or unable to palpate it.Opportunistic screening for AF using optimal pulse palpation in candidate individuals according to a simple algorithm may have high effectiveness in detecting AF in primary care

Accurate Atmospheric Parameters at Moderate Resolution Using Spectral Indices: Preliminary Application to the MARVELS Survey

Studies of Galactic chemical and dynamical evolution in the solar neighborhood depend on the availability of precise atmospheric parameters (Teff, [Fe/H] and log g) for solar-type stars. Many large-scale spectroscopic surveys operate at low to moderate spectral resolution for efficiency in observing large samples, which makes the stellar characterization difficult due to the high degree of blending of spectral features. While most surveys use spectral synthesis, in this work we employ an alternative method based on spectral indices to determine the atmospheric parameters of a sample of nearby FGK dwarfs and subgiants observed by the MARVELS survey at moderate resolving power (R~12,000). We have developed three codes to automatically normalize the observed spectra, measure the equivalent widths of the indices and, through the comparison of those with values calculated with pre-determined calibrations, derive the atmospheric parameters of the stars. The calibrations were built using a sample of 309 stars with precise stellar parameters obtained from the analysis of high-resolution FEROS spectra. A validation test of the method was conducted with a sample of 30 MARVELS targets that also have reliable atmospheric parameters from high-resolution spectroscopic analysis. Our approach was able to recover the parameters within 80 K for Teff, 0.05 dex for [Fe/H] and 0.15 dex for log g, values that are lower or equal to the typical external uncertainties found between different high-resolution analyzes. An additional test was performed with a subsample of 138 stars from the ELODIE stellar library and the literature atmospheric parameters were recovered within 125 K for Teff, 0.10 dex for [Fe/H] and 0.29 dex for log g. These results show that the spectral indices are a competitive tool to characterize stars with the intermediate resolution spectra.Comment: Accepted for publication in AJ. Abstract edited to comply with arXiv standards regarding the number of character

Abundance to age ratios in the HARPS-GTO sample with Gaia DR2: Chemical clocks for a range of [Fe/H]

[ABRIDGED] The purpose of this work is to evaluate how several elements produced by different nucleosynthesis processes behave with stellar age and provide empirical relations to derive stellar ages from chemical abundances. We derive different sets of ages using Gaia parallaxes for a sample of more than 1000 FGK dwarf stars for which he have spectra from the HARPS-GTO program. We analyze the temporal evolution of different abundance ratios to find the best chemical clocks. We find that [$\alpha$/Fe] ratio (average of Mg, Si and Ti), [O/Fe] and [Zn/Fe] are good age proxies with a lower dispersion than the age-metallicity dispersion. Several abundance ratios present a significant correlation with age for chemically separated thin disk stars (i.e. low-$\alpha$) but in the case of the chemically defined thick disk stars (i.e. high-$\alpha$) only the elements Mg, Si, Ca and TiII show a clear correlation with age. We find that the thick disk stars are more enriched in light-s elements than thin disk stars of similar age. The maximum enrichment of s-process elements in the thin disk occurs in the youngest stars which in turn have solar metallicity. The slopes of the [X/Fe]-age relations are quite constant for O, Mg, Si, Ti, Zn, Sr and Eu regardless of the metallicity. However, this is not the case for Al, Ca, Cu and most of the s-process elements, which display very different trends depending on the metallicity. This demonstrates the limitations of using simple linear relations based on certain abundance ratios to obtain ages for stars of different metallicities. Finally, we show that by using 3D relations with a chemical clock and two stellar parameters (either Teff, [Fe/H] or stellar mass) we can explain up to 89% of age variance in a star. A similar result is obtained when using 2D relations with a chemical clock and one stellar parameter, being up to a 87% of the variance explained.Comment: accepted for publication in A&A, version after language revisio