Screening of Aflatoxin B1 in Laboratory Rat Feed

Many hazards can interfere with the safety of the feeding stuffs intended to provide nutrients to experimental  animals. Chemical and biological contaminants of laboratory animal feed can be a problem for toxicological  and immunological research. Aflatoxin B1 (AFB1) is a secondary toxic metabolite, produced by  the ubiquitous fungal genera, Aspergillus. AFB1 is particularly dangerous for health, inducing cancer of the  urinary tract or liver carcinoma. The aim of this preliminary screening was to evaluate the presence of AFB1  in 31 samples of laboratory rat feed using a method validated in-house, then separated by High Pressure  Liquid Chromatography (HPLC) coupled to a fluorescence detector. The detection limit (limit of detection:  LOD) and the quantification limit (LOQ) were 0.2 and 0.4 ìg/kg respectively. Recoveries ranged from 58.0  to 74.5% for spiked samples. The immunoaffinity approach was significantly faster than methods employing  conventional chromatography clean-up (Sep-Pak Classic Florisil and Sep-Pak ClassicC18 cartridges).  Aflatoxins were not detected in any analysed sample.

Identificação de vias de transição agroecológica com base na Abordagem Global da Exploração Agrícola: o caso dos criadores de gado familiares no norte do Uruguai

Agroecology represents one of the main alternatives to production models generated by the green revolution, both in Uruguay and other countries. It appears as a response to climate change, biodiversity management, nature resource restoration, and, more recently, as a response to the COVID-19 pandemic crisis. In Uruguay, agroecology is marginal despite the existence of a law for a National Plan to Encourage Production with Agroecological Bases since 2018. Moreover, research on agroecological transition processes is very incipient. This study aims to identify agroecological practices and possible transition paths towards agroecology of family livestock farmers in northern Uruguay. The methodology used focuses the study on technical, social and organizational practices, allowing access to the operation scheme and understanding the decision-making processes in family-type production systems. The socio-productive practices revealed in the research allowed the elaboration of a typology that represents possible ways for these livestock farmers to move towards agroecological production and management of their farms. The results of this study can be used to strengthen processes of conception or co-conception by selecting and disseminating innovation with actions and public policies closer to farmers.La agroecología representa una de las principales alternativas a modos de producción generados por la revolución verde, tanto en Uruguay como en otros países. Aparece como una respuesta al cambio climático, a la gestión de la biodiversidad y a la restauración de los recursos naturales; y más recientemente como respuesta a la crisis de la pandemia COVID-19. En Uruguay la agroecología, a pesar de que existe una Ley de Plan Nacional para el Fomento de la Producción con Bases Agroecológicas desde 2018, es marginal y la investigación sobre procesos de transición agroecológica es muy incipiente. El objetivo de este trabajo es identificar prácticas agroecológicas y posibles caminos de transición hacia la agroecología de los ganaderos familiares del norte uruguayo utilizando una metodología que se centra en el estudio de las prácticas técnicas, sociales y organizativas, lo cual permite acceder al esquema de funcionamiento y comprender los procesos de toma de decisiones en los sistemas de producción de tipo familiar. Las prácticas socio-productivas evidenciadas en la investigación permitieron elaborar una tipología que representa posibles caminos por los cuales estos ganaderos transitan hacia una producción y gestión agroecológica de las explotaciones. Los resultados de este estudio pueden ser utilizados para fortalecer procesos de concepción o de co-concepción, a través de la selección y difusión de innovaciones con acciones y políticas públicas más cercanas a los productores.A agroecologia representa uma das principais alternativas aos modos de produção gerados pela revolução verde, tanto no Uruguai como em outros países. Aparece como uma resposta às alterações climáticas, à gestão da biodiversidade e à restauração dos recursos naturais; e mais recentemente como uma resposta à crise pandêmica da COVID-19. No Uruguai, a agroecologia é marginal, apesar da existência de uma Lei do Plano Nacional para a Promoção da Produção de Base Agroecológica desde 2018, e a investigação sobre processos de transição agroecológica é muito incipiente. O objetivo deste trabalho é identificar práticas agroecológicas e possíveis vias de transição para a agroecologia dos criadores de gado familiares no norte do Uruguai, utilizando uma metodologia centrada no estudo das práticas técnicas, sociais e organizacionais, que permite o acesso ao esquema de funcionamento e a compreensão dos processos de tomada de decisão em sistemas de produção de tipo familiar. As práticas socio-produtivas reveladas pela investigação permitiram elaborar uma tipologia que representa as possíveis formas de evolução destes criadores de gado para a produção e gestão agro-ecológica das suas explorações. Os resultados deste estudo podem ser utilizados para reforçar os processos de concepção ou co-concepção, através da seleção e divulgação de inovações com ações e políticas públicas mais próximas dos produtores

Pediatric hepatic hemangioendothelioma: clinical and imaging characteristics. Case report and literature review

Se presenta un caso de hemangioendotelioma (HE) hepático infantil en un recién nacido masculino quien consultó por presentar síntomas de falla cardíaca. Se hace revisión de sus manifestaciones clínicas y sus características imagenológicas. [Ferreira HH, Archila MM. Hemangioendotelioma hepático infantil: características clínicas e imaginológicas. Presentación de un caso y revisión de la literaturaWe report a case of infantile hepatic hemangioendothelioma (HE) in a newborn male with congestive heart failure. We review the clinical and radiological findings. [Ferreira HH, Archila MM. infan-tile hepatic hemangioendothelioma: Clinical and imaginological characteristics. Case presentation and literature review. MedUNA

Role of serotiny on Pinus pinaster Aiton germination and its relation to mother plant age and fire severity

erotiny degree may hypothetically depend on mother plant age and fire severity, and fire severity can strongly affect the regeneration of Pinus pinaster Aiton, which is an obligate seeder species with aerial seed banks stocked in cones showing a certain degree of serotiny. The main objective of this study was to evaluate the effect of serotiny and mother plant age on seed germination of P. pinaster in relation to fire severity. For this purpose, cones were collected in two stands from mother trees of different ages. Serotinous cones were opened in oven at increasing temperatures to determine the number of seeds released. The length of cones and the number and weight of seeds were measured. Seeds from both classes of mother plant age were subjected to 34 different treatments simulating different fire severity levels, and their germination was recorded. The results showed that serotiny was significantly higher in cones from young mother trees. Seed germination in the control groups from serotinous cones was always higher than that from non-serotinous cones without thermal shock. Fire severity decreased seed germination as fire severity increases in both mother age classes. Mother plant age was related to the serotiny level, which was higher in young than in old trees, suggesting a strong selection in the population due to a recent fire.This work is part of the research projects AGL2013-48189-C2-2-R (Ministerio de Economía y Competividad, Madrid, Spain, and ERDF founding), AGL2017-86075-C2-2-R (Ministerio de Ciencia, Educación y Universidades, Madrid, Spain, and ERDF founding), LE001P17 (Junta de Castilla y León, Valladolid, Spain), ED431C2019/07 and ED431E 2018/09 (Xunta de Galicia, Santiago de Compostela, Spain)S

Structural invariance of multiple intelligences, based on the level of execution

The independence of multiple intelligences (MI) of Gardner’s theory has been debated since its conception. This article examines whether the one- factor structure of the MI theory tested in previous studies is invariant for low and high ability students. Two hundred ninety-four children (aged 5 to 7) participated in this study. A set of Gardner’s Multiple Intelligence assessment tasks based on the Spectrum Project was used. To analyze the invariance of a general dimension of intelligence, the different models of behaviours were studied in samples of participants with different performance on the Spectrum Project tasks with Multi-Group Confi rmatory Factor Analysis (MGCFA). Results suggest an absence of structural invariance in Gardner’s tasks. Exploratory analyses suggest a three-factor structure for individuals with higher performance levels and a two-factor structure for individuals with lower performance levels

São Camilo de Léllis: Caridade e bondade na prestação do cuidado

O objeto do estudo é a representação pictórica do cuidado prestado por Camilo de Léllis, tendo por objetivo: apresentar a trajetória de vida de Camilo de Lellis; mostrar os cuidados prestados por Camilo de Léllis, por meio da representação pictórica de Pierre Hubert Subleyras (1745) e comentar a trajetória dos cuidados por ele prestada, como contribuição para a construção do conhecimento da História da Enfermagem. Método: Os passos seguidos para atendimento da investigação foram à busca de literatura de aderência articulada à representação pictórica proposta. Isto implicou, em síntese, na apresentação da biografia de Camilo de Léllis e a decodificação dos atributos em tela analisada. O resultado evidenciou o cuidado, para além do corpo físico, mas da alma, na cura das enfermidades com normas e sistematização no século XVIII.El objeto de estudio es la representación pictórica de los cuidados prestados por Camilo de Lelis, teniendo como objetivo: presentar la trayectoria de vida de Camillus de Lellis; mostrar los cuidados realizados por Camilo de Léllis, por medio de la representación pictórica del Pierre Hubert Subleyras (1745) y comentar su trayectoria de los cuidados como contribución a la construcción del conocimiento de la historia de la enfermería. Método: los pasos seguidos para la investigación se ciñeron a la búsqueda de la literatura de la propuesta de adhesión articulada representación pictórica propuesta. Esto exigió, en resumen, la presentación de la biografía de Camilo de Léllis y la descodificación de los atributos de pantalla examinados. El resultado mostró el cuidado, además del cuerpo físico, sino el alma, la curación de enfermedades con estándares y sistematización en el siglo XVIII.The object of this study is the pictorial representation of the care provided by Camilo de Léllis, which has as objective: to present the life trajectory of Camillus de Lellis; to show the care provided by Camilo de Léllis, through the pictorial representation of Pierre Hubert Subleyras (1745) and comment the trajectory of caring given by him, as a contribution to the construction of the knowledge of the history of nursing. Method: steps followed for the investigation were the search of the literature of the articulated pictorial representation adhesion proposal. This entailed, in summary, a presentation of a biography of Camilo de Léllis and the decoding of the screen attributes examined. The result showed a care of the soul, beyond the physical body, in healing of illnesses with standards and systematization in the 18th century

The Covid-19 explosion in the state of Amapá: how is the most preserved region in the Brazilian Amazon currently fighting the SARS-COV 2 pandemic?

The state of Amapá is located in the extreme north of Brazil, within the Amazon rainforest and is crossed by the Equator. It has a hot and humid climate with rains that last 8 months a year and 4 months of unrelenting sun that melts rubber from car seals, fries eggs on the floor and even cooks a whole egg tub, in case you forget in a car exposed to the sun . It was believed that with this potent solar incidence, the Sars-COV 2 virus would not have so much impact in this region, a terrible mistake! Today Amapá has the highest incidence of Covid-19 in the whole of Brazil, with a maid of 600 cases per hundred thousand inhabitants and in the Amazon it is the 3rd in deaths and loses in this item only to the state of Amazonas and Pará.The state of Amapá is located in the extreme north of Brazil, within the Amazon rainforest and is crossed by the Equator. It has a hot and humid climate with rains that last 8 months a year and 4 months of unrelenting sun that melts rubber from car seals, fries eggs on the floor and even cooks a whole egg tub, in case you forget in a car exposed to the sun . It was believed that with this potent solar incidence, the Sars-COV 2 virus would not have so much impact in this region, a terrible mistake! Today Amapá has the highest incidence of Covid-19 in the whole of Brazil, with a maid of 600 cases per hundred thousand inhabitants and in the Amazon it is the 3rd in deaths and loses in this item only to the state of Amazonas and Pará

Fourth International Conference on Sustainable Tourism Planet, People, Peace (P3)

El texto relata los principales puntos de discusión presentados en la IV Conferencia Internacional de Turismo Sostenible Planeta, Personas, Paz; con énfasis en los desafíos que presenta el desarrollo del turismo desde una perspectiva de sostenibilidad. Se presenta también la estructura y concepto del evento.El texto relata los principales puntos de discusión presentados en la IV Conferencia Internacional de Turismo Sostenible Planeta, Personas, Paz; con énfasis en los desafíos que presenta el desarrollo del turismo desde una perspectiva de sostenibilidad. Se presenta también la estructura y concepto del evento.The following text recounts the main discussions presented in the IV International Conference on Sustainable Tourism Planet, People, Peace; emphasizing in the challenges of tourism development from a sustainability perspective. It also provides the structure and concept of the event

From prima donna to teacher. Two female pioneers in singing education in the Nineteenth Century: Virginia Boccabadati and Matilde Esteban

During the 19th-century, the teaching of singing became a career opportunity for the women who had worked in the lyrical stage. The core objective of this article is to reclaim the role of women in music education by studying two prime donnewho, after building a career as successful professional singers, became singing teachers and were pioneers in publishing their methods for female voice education. This research is based on the review and analysis of documentation, mostly historical. By looking at the biographies of the Italian singer, Virginia Boccabadati (1830-1922) and the Spanish singer, Matilde Esteban (1841-1915), we can discover the context in which their treatises were published and the image they offer of woman as a singer and as a student. By choosing women who were each other's contemporary, but from different countries, helps us to observe the obvious points related to gender determinants that their treatises had in common