208 research outputs found
Comparative QSAR analyses of competitive CYP2C9 inhibitors using three-dimensional molecular descriptors
One of the biggest challenges in QSAR studies
using three-dimensional descriptors is to generate
the bioactive conformation of the molecules. Com parative QSAR analyses have been performed on a
dataset of 34 structurally diverse and competitive
CYP2C9 inhibitors by generating their lowest
energy conformers as well as additional multiple
conformers for the calculation of molecular de scriptors. Three-dimensional descriptors account ing for the spatial characteristics of the molecules
calculated using E-Dragon were used as the inde pendent variables. The robustness and the predic tive performance of the developed models were
verified using both the internal [leave-one-out
(LOO)] and external statistical validation (test set
of 12 inhibitors). The best models (MLR using GET AWAY descriptors and partial least squares using
3D-MoRSE) were obtained by using the multiple
conformers for the calculation of descriptors and
were selected based upon the higher external pre diction (R2
test values of 0.65 and 0.63, respectively)
and lower root mean square error of prediction
(0.48 and 0.48, respectively). The predictive ability
of the best model, i.e., MLR using GETAWAY de scriptors was additionally verified on an external
test set of quinoline-4-carboxamide analogs and
resulted in an R2
test value of 0.6. These simple and
alignment-independent QSAR models offer the
possibility to predict CYP2C9 inhibitory activity of
chemically diverse ligands in the absence of X-ray
crystallographic information of target protein
structure and can provide useful insights about
the ADMET properties of candidate molecules in
the early phases of drug discovery.info:eu-repo/semantics/publishedVersio
QSAR models for prediction of PPARδ agonistic activity of indanylacetic acid derivatives
Peroxisome Proliferator Activated Receptor b/d (PPAR b/d), one of three PPAR isoforms
is a member of nuclear receptor superfamily and ubiquitously expressed in several
metabolically active tissues such as liver, muscle, and fat. Tissue specific expression and
knock-out studies suggest a role of PPARd in obesity and metabolic syndrome. Specific
and selective PPARd ligands may play an important role in the treatment of metabolic
disorders. Indanylacetic acid derivatives reported as potent and specific ligands against
PPARd have been studied for the Quantitative Structure – Activity Relationships
(QSAR). Molecules were represented by chemical descriptors that encode constitutional,
topological, geometrical, and electronic structure features. Four different approaches, i.e.,
random selection, hierarchical clustering, k-means clustering, and sphere exclusion
method were used to classify the dataset into training and test subsets. Forward stepwise
Multiple Linear Regression (MLR) approach was used to linearly select the subset of
descriptors and establish the linear relationship with PPARd agonistic activity of the
molecules. The models were validated internally by Leave One Out (LOO) and externally
for the prediction of test sets. The best subset of descriptors was then fed to the Artificial
Neural Networks (ANN) to develop non-linear models. Statistically significant MLR
models; with R2 varying from 0.80 to 0.87 were generated based on the different training
and test set selection methods. Training of ANNs with different architectures for the
different training and test selection methods resulted in models with R2 values varying
from 0.83 to 0.94, which indicates the high predictive ability of the models.info:eu-repo/semantics/publishedVersio
SitCon: binding site residue conservation visualization and protein sequence-to-function tool
We introduce SitCon (SITe CONservation), a program designed to explore
conservation of functionally important sites in a series of hypothetically homologous
candidate protein structures, given amino acid sequence as an input. This can especially be
useful when looking for an unknown function of a protein. SitCon exploits the fact that
binding sites of proteins are preserved better than the overall residue sequence
conservation. To test the capability of unknown function prediction, we randomly chose
known function proteins from Caenorhabditis elegans genome. To imitate a behavior of an
unknown function target, only the low homology proteins with 0.01 E-score 100 were
analyzed as templates. Out of 29 enzyme targets, SitCon was able to provide various hints
about their function in at least 69% of the cases. For the eight nonenzyme targets, the
predictions matched in only 25% of the cases. SitCon was also tested for a capability to
predict presence or absence of metal-containing heterogroups in the target enzymes with
80% success rate. Because this algorithm is not based on specific protein signatures, it
may allow detection of overlooked relationships between proteins. SitCon is also very
effective as a tool allowing visual comparison of binding site residue conservation between
the target and homologous templates side-by-side.info:eu-repo/semantics/publishedVersio
Quantitative structure-activity relationship models with receptor-dependent descriptors for predicting peroxisome proliferator-activated receptor activities of thiazolidinedione and oxazolidinedione derivatives
A quantitative structure–activity relationship study
has been carried out, in which the relationship
between the peroxisome proliferator-activated
receptor a and the peroxisome proliferator activated receptor c agonistic activities of thiazo lidinedione and oxazolidinedione derivatives and
quantitative descriptors, Vsite calculated in a
receptor-dependent manner is modeled. These
descriptors quantify the volume occupied by the
optimized ligands in regions that are either com mon or specific to the superimposed binding sites
of the targets under consideration. The quantita tive structure–activity relationship models were
built by forward stepwise linear regression model ing for a training set of 27 compounds and vali dated for a test set of seven compounds, resulting
in a squared correlation coefficient value of 0.90
for peroxisome proliferator-activated receptor a
and of 0.89 for peroxisome proliferator-activated
receptor c. The leave-one-out cross-validation and
test set predictability squared correlation coeffi cient values for these models were 0.85 and 0.62
for peroxisome proliferator-activated receptor a
and 0.89 and 0.50 for peroxisome proliferator-acti vated receptor c respectively. A dual peroxisome
proliferator-activated receptor model has also
been developed, and it indicates the structural
features required for the design of ligands with
dual peroxisome proliferator-activated receptor
activity. These quantitative structure–activity
relationship models show the importance of the
descriptors here introduced in the prediction
and interpretation of the compounds affinity and
selectivity.info:eu-repo/semantics/publishedVersio
Generation of artificial neural networks models in anticancer study
Artificial neural networks (ANNs) have several
applications; one of them is the prediction of biological
activity. Here, ANNs were applied to a set of 32 compounds
with anticancer activity assayed experimentally against two
cancer cell lines (A2780 and T-47D). Using training and
test sets, the obtained correlation coefficients between
experimental and calculated values of activity, for A2780,
were 0.804 and 0.829, respectively, and for T-47D, we got
0.820 for the training set and 0.927 for the test set. Com paring multiple linear regression and ANN models, the
latter were better suited in establishing relationships
between compounds’ structure and their anticancer activity.info:eu-repo/semantics/publishedVersio
Caffeoylquinic acids as inhibitors for HIV-I protease and HIV-I integrase: a molecular docking study
Caffeoylquinic acids are ubiquitous phenolic compounds with several health benefits to humans and they
have been shown to be promisinganti-HIV compounds. In this work, molecular docking was used to study
the inhibition of HIV-I integrase and protease using caffeoylquinic acids. It was possible to establish that the
naturally occurring caffeoylquinic acids are not suitable as inhibitors for protease but are very good
inhibitors for integrase. A new binding site was found for 3, 4-O-di-Caffeoylquinic acid between the chains of
HIV-I integrase that could possibly lead to a disruption of the catalytic process of HIV-I integrase.info:eu-repo/semantics/publishedVersio
Protein-ligand docking study: diterpenes from Juniperus brevifolia as anticancer and antimicrobial agentes
REDCAT: Natural Products and related Redox Catalysts: Basic Research and Applications in Medicine and Agriculture, Aveiro, 25-27 Novembro de 2012.From leaves of Juniperus brevifolia, an endemic conifer from Azores, were isolated and structurally characterized, several dehydroabietane and sandaracopimarane derivatives. Some of them (1-4), displayed antiproliferative activity against cancer cell lines (HeLa, A-549 and MCF-7) and bactericidal effect against Bacillus cereus at different concentrations tested. However, it is not known how these compounds interact with most often proteins involved in the antimicrobial and cytotoxic mechanisms. Protein-ligand docking is mainly used to predict (energy and conformation wise) how small molecules bind to a protein of known 3D structure and to predict possible molecular targets for a set of compounds. In this work, the docking studies were performed, using the FlexScreen program, in order to pick molecular targets from a large set of common anticancer (63) and antimicrobial (39) targets to the selected compounds 1-4. The predicted interactions established between the compounds under study and the anticancer targets revealed that the compounds 1 and 3 interact preferentially with phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 2, whereas compounds 2 and 4 interact preferentially with human mitochondrial peptide deformylase and -tubulin, respectively. Studying the interactions between the compounds 1 and 3 and the antimicrobial targets we predict that these compounds interact preferentially with RNA polymerase and peptide deformylase. These results provide additional understanding of the cytotoxic and antimicrobial effects of diterpenes studied. These preliminary computational docking predictions of therapeutic targets were established working with just 4 compounds, and to obtain more reliable predictions the number of compounds needs to be increased.Thanks are due to the University of Azores, FCT, FEDER, BIOPHARMAC - MAC/1/C104 and Project PEst-OE/QUI/UI0674/2011
Prediction of terpenoid toxicity based on a quantitative structure–activity relationship model
Terpenoids, including monoterpenoids (C10), norisoprenoids (C13), and sesquiterpenoids
(C15), constitute a large group of plant-derived naturally occurring secondary metabolites with
highly diverse chemical structures. A quantitative structure–activity relationship (QSAR) model to
predict terpenoid toxicity and to evaluate the influence of their chemical structures was developed in
this study by assessing in real time the toxicity of 27 terpenoid standards using the Gram-negative
bioluminescent Vibrio fischeri. Under the test conditions, at a concentration of 1 µM, the terpenoids
showed a toxicity level lower than 5%, with the exception of geraniol, citral, (S)-citronellal, geranic
acid, (±)-α-terpinyl acetate, and geranyl acetone. Moreover, the standards tested displayed a
toxicity level higher than 30% at concentrations of 50–100 µM, with the exception of (+)-valencene,
eucalyptol, (+)-borneol, guaiazulene, β-caryophellene, and linalool oxide. Regarding the functional
group, terpenoid toxicity was observed in the following order: alcohol > aldehyde ~ ketone >
ester > hydrocarbons. The CODESSA software was employed to develop QSAR models based on
the correlation of terpenoid toxicity and a pool of descriptors related to each chemical structure.
The QSAR models, based on t-test values, showed that terpenoid toxicity was mainly attributed
to geometric (e.g., asphericity) and electronic (e.g., maximum partial charge for a carbon (C) atom
(Zefirov’s partial charge (PC)) descriptors. Statistically, the most significant overall correlation was the
four-parameter equation with a training coefficient and test coefficient correlation higher than 0.810
and 0.535, respectively, and a square coefficient of cross-validation (Q2
) higher than 0.689. According
to the obtained data, the QSAR models are suitable and rapid tools to predict terpenoid toxicity in a
diversity of food products.info:eu-repo/semantics/publishedVersio
Phosphorylation of mineralocorticoid receptor ligand binding domain impairs receptor activation and has a dominant negative effect over non-phosphorylated receptors
Post-translational modification of steroid receptors allows
fine-tuning different properties of this family of proteins,
including stability, activation, or interaction with co-regulators.
Recently, a novel effect of phosphorylation on steroid receptor
biology was described. Phosphorylation of human mineralocor ticoid receptor (MR) on Ser-843, a residue placed on the ligand
binding domain, lowers affinity for agonists, producing inhibi tion of gene transactivation. We now show that MR inhibition
by phosphorylation occurs even at high agonist concentration,
suggesting that phosphorylation may also impair coupling
between ligand binding and receptor activation. Our results
demonstrate that agonists are able to induce partial nuclear
translocation of MR but fail to produce transactivation due at
least in part to impaired co-activator recruitment. The inhibi tory effect of phosphorylation on MR acts in a dominant-nega tive manner, effectively amplifying its functional effect on gene
transactivation.info:eu-repo/semantics/publishedVersio
Structure and ligand-based design of P-glycoprotein inhibitors: a historical perspective
Computer-assisted drug design (CADD) is a valuable approach for the discovery of new chemical entities in the field of cancer
therapy. There is a pressing need to design and develop new, selective, and safe drugs for the treatment of multidrug resistance (MDR)
cancer forms, specifically active against P-glycoprotein (P-gp). Recently, a crystallographic structure for mouse P-gp was obtained.
However, for decades the design of new P-gp inhibitors employed mainly ligand-based approaches (SAR, QSAR, 3D-QSAR and phar macophore studies), and structure-based studies used P-gp homology models. However, some of those results are still the pillars used as a
starting point for the design of potential P-gp inhibitors. Here, pharmacophore mapping, (Q)SAR, 3D-QSAR and homology modeling,
for the discovery of P-gp inhibitors are reviewed. The importance of these methods for understanding mechanisms of drug resistance at a
molecular level, and design P-gp inhibitors drug candidates are discussed. The examples mentioned in the review could provide insights
into the wide range of possibilities of using CADD methodologies for the discovery of efficient P-gp inhibitors.info:eu-repo/semantics/publishedVersio
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