Oral alterations and oral care in bone marrow transplant patients

Os cuidados gerais relativos ao paciente submetido ao transplante de medula óssea (TMO) incluem avaliações odontológicas rotineiras, as quais devem estar inseridas em um contexto multiprofissional. A cavidade oral constitui um sítio propício a infecções com grande potencial de desenvolvimento de bacteremia, sendo que lesões infecciosas devem ser previamente tratadas e controladas pelo cirurgião-dentista. O objetivo desta revisão é discutir questões em destaque na literatura nacional e internacional referentes aos quadros inflamatórios e infecciosos orais de importância para o paciente transplantado de medula óssea, tanto os predisponentes a complicações durante o transplante, quanto os que ocorrem durante e após a terapia mielossupressora. Destaca-se na literatura a doença periodontal avançada, a qual constitui um quadro infeccioso crônico que deve ser evitado ou controlado durante o TMO, principalmente devido à presença de S. viridans. Os fatores de risco para mucosite oral (OM), doença do enxerto contra o hospedeiro (DECH) e xerostomia ainda não estão definidos, principalmente para OM e DECH. São citadas na literatura alternativas promissoras de tratamento para OM, tais como crioterapia, administração de fatores de crescimento e laserterapia. O risco aumentado de cárie é controverso e, dentre as lesões fúngicas e virais, destacam-se as infecções orais e de orofaringe por Candida e pela família de herpesvírus, de importância clínica considerável. Em pacientes pediátricos são relevantes as alterações craniofaciais e dentárias, decorrentes principalmente da radioterapia.General care in bone marrow transplant (BMT) patients includes routine dental evaluations, which must be included in a multi-professional context. The oral cavity is a site that favors infections with high potential for consequent bacteremia and so infectious lesions must be treated or controlled by the dentist. The aim of this review is to discuss key questions in national and international literature with reference to oral inflammatory conditions of BMT patients, both those with predisposition to complications during the transplant and those that emerge during and after myelosuppression therapy. The literature emphasizes advanced periodontal disease, which is a chronic infectious condition that must be avoided or controlled during BMT, particularly because of the presence of S. viridans. The risk factors for oral mucositis, graft-versus-host disease (GVHD) and xerostomia have not yet been defined, particularly for oral mucositis and GVHD. Promising alternatives in the treatment of oral mucositis, including cryotherapy, administration of growth factors and laser therapy, are emphatically mentioned. In children, craniofacial and dental alterations, particularly resulting from radiotherapy are relevant. The increased risk of caries is controversial, and among fungal and viral lesions, oral and oropharyngeal infections by Candida and the herpes virus are identified as being of considerable clinical importance

Red Iberoamericana de Toxicología y Seguiridad Química

La Red Iberoamericana de Toxicología y Seguridad Química RITSQ, se inicia en marzo de 2008 y desde entonces ha tenido 67.479 visitas a la página web de la misma, se han registrado 1.133 personas de 41 países y desde entonces hemos realizado y presentado 57 carteles en Reuniones, Conferencias y Reuniones donde se mantienen de forma constante los Objetivos de la RITSQ: 1. Coordinar la participación de los diferentes grupos existentes en universidades y organismos de investigación de Iberoamérica, implicados en estudios relacionados con la Toxicología, 2. Fortalecer la colaboración y el intercambio académico entre los programas de Doctorado y Maestría de diferentes países iberoamericanos que tengan como objeto el estudio y la investigación en Toxicología o áreas relacionadas, 3. Favorecer la realización de proyectos de investigación conjuntos entre docentes e investigadores de Iberoamérica, pasantías estudiantiles y eventos académicos; 4. Profundizar en el estudio de métodos de ensayo de corta y larga duración utilizados en la evaluación de la carcinogenicidad, la mutagenicidad y la toxicidad para la reproducción de sustancias y mezclas de productos químicos, 5. Desarrollar y estandarizar métodos analíticos para la identificación y determinación de biomarcadores de exposición, efecto y .susceptibilidad para sustancias y productos químicos en el hombre y el medio ambiente; 6. Aplicar métodos de evaluación del riesgo para la salud humana y el medio ambiente de sustancias y productos químicos, 7. Fomentar el intercambio científico de profesionales interesados alimentaria; y 8. Propiciar el uso de métodos alternativos a la experimentación animal (www.remanet.net).Peer reviewe

Red Iberoamericana de Toxicología y Seguridad Química

La Red Iberoamericana de Toxicología y Seguridad Química RITSQ, se inicia en marzo de 2008 y desde entonces ha tenido 69.930 visitas a la página web de la misma, se han registrado 1.133 personas de 41 países y desde entonces hemos realizado y presentado 66 carteles en Reuniones, Conferencias y Reuniones donde se mantienen de forma constante los Objetivos de la RITSQ: 1. Coordinar la participación de los diferentes grupos existentes en universidades y organismos de investigación de Iberoamérica, implicados en estudios relacionados con la Toxicología, 2. Fortalecer la colaboración y el intercambio académico entre los programas de Doctorado y Maestría de diferentes países iberoamericanos que tengan como objeto el estudio y la investigación en Toxicología o áreas relacionadas, 3. Favorecer la realización de proyectos de investigación conjuntos entre docentes e investigadores de Iberoamérica, pasantías estudiantiles y eventos académicos; 4. Profundizar en el estudio de métodos de ensayo de corta y larga duración utilizados en la evaluación de la carcinogenicidad, la mutagenicidad y la toxicidad para la reproducción de sustancias y mezclas de productos químicos, 5. Desarrollar y estandarizar métodos analíticos para la identificación y determinación de biomarcadores de exposición, efecto y .susceptibilidad para sustancias y productos químicos en el hombre y el medio ambiente; 6. Aplicar métodos de evaluación del riesgo para la salud humana y el medio ambiente de sustancias y productos químicos, 7. Fomentar el intercambio científico de profesionales interesados alimentaria; y 8. Propiciar el uso de métodos alternativos a la experimentación animal (www.remanet.net).Peer reviewe

Red Iberoamericana de Toxicología y Seguridad Química

La Red Iberoamericana de Toxicología y Seguridad Química RITSQ, se inicia en marzo de 2008 y desde entonces ha tenido 70.104 visitas a la página web de la misma, se han registrado 1.133 personas de 41 países y desde entonces hemos realizado y presentado 66 carteles en Reuniones, Conferencias y Reuniones donde se mantienen de forma constante los Objetivos de la RITSQ: 1. Coordinar la participación de los diferentes grupos existentes en universidades y organismos de investigación de Iberoamérica, implicados en estudios relacionados con la Toxicología, 2. Fortalecer la colaboración y el intercambio académico entre los programas de Doctorado y Maestría de diferentes países iberoamericanos que tengan como objeto el estudio y la investigación en Toxicología o áreas relacionadas, 3. Favorecer la realización de proyectos de investigación conjuntos entre docentes e investigadores de Iberoamérica, pasantías estudiantiles y eventos académicos; 4. Profundizar en el estudio de métodos de ensayo de corta y larga duración utilizados en la evaluación de la carcinogenicidad, la mutagenicidad y la toxicidad para la reproducción de sustancias y mezclas de productos químicos, 5. Desarrollar y estandarizar métodos analíticos para la identificación y determinación de biomarcadores de exposición, efecto y .susceptibilidad para sustancias y productos químicos en el hombre y el medio ambiente; 6. Aplicar métodos de evaluación del riesgo para la salud humana y el medio ambiente de sustancias y productos químicos, 7. Fomentar el intercambio científico de profesionales interesados alimentaria; y 8. Propiciar el uso de métodos alternativos a la experimentación animal (www.remanet.net).Peer reviewe

The molecular environment of the pillar-like features in the H ii region G46.5–0.2

At the interface of HII regions and molecular gas, peculiar structuresappear, some of them with pillar-like shapes. Understanding their originis important for characterizing triggered star formation and the impactof massive stars on the interstellar medium. In order to study themolecular environment and influence of radiation on two pillar-likefeatures related to the H ii region G46.5-0.2, we performed molecularline observations with the Atacama Submillimeter Telescope Experimentand spectroscopic optical observations with the Isaac Newton Telescope.From the optical observations, we identified the star that is excitingthe H ii region as spectral type O4-6. The molecular data allowed us tostudy the structure of the pillars and an HCO+ cloud lyingbetween them. In this HCO+ cloud, which has no well-defined12CO counterpart, we found direct evidence of star formation:two molecular outflows and two associated near-IR nebulosities. Theoutflow axis orientation is perpendicular to the direction of theradiation flow from the HII region. Several Class I sources are alsoembedded in this HCO+ cloud, showing that it is usual thatyoung stellar objects (YSOs) form large associations occupying a cavitybounded by pillars. On the other hand, it was confirmed that theradiation-driven implosion (RDI) process is not occurring in one of thepillar tips.Fil: Paron, Sergio Ariel. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Celis Peña, Mariela Fernanda. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Ortega, Martin Eduardo. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Fariña, Cecilia Silvia. Isaac Newton Group Of Telescopes, Las Palmas, Iac; EspañaFil: Petriella, Alberto. Consejo Nacional de Investigaciónes Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Astronomía y Física del Espacio. - Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Astronomía y Física del Espacio; ArgentinaFil: Rubio, M.. Departamento de Astronomía, Universidad de Chile; ChileFil: Ashley, R. P.. University Of Warwick; Reino Unid

Androgen responsive intronic non-coding RNAs

BACKGROUND: Transcription of large numbers of non-coding RNAs originating from intronic regions of human genes has been recently reported, but mechanisms governing their biosynthesis and biological functions are largely unknown. In this work, we evaluated the existence of a common mechanism of transcription regulation shared by protein-coding mRNAs and intronic RNAs by measuring the effect of androgen on the transcriptional profile of a prostate cancer cell line. RESULTS: Using a custom-built cDNA microarray enriched in intronic transcribed sequences, we found 39 intronic non-coding RNAs for which levels were significantly regulated by androgen exposure. Orientation-specific reverse transcription-PCR indicated that 10 of the 13 were transcribed in the antisense direction. These transcripts are long (0.5–5 kb), unspliced and apparently do not code for proteins. Interestingly, we found that the relative levels of androgen-regulated intronic transcripts could be correlated with the levels of the corresponding protein-coding gene (asGAS6 and asDNAJC3) or with the alternative usage of exons (asKDELR2 and asITGA6) in the corresponding protein-coding transcripts. Binding of the androgen receptor to a putative regulatory region upstream from asMYO5A, an androgen-regulated antisense intronic transcript, was confirmed by chromatin immunoprecipitation. CONCLUSION: Altogether, these results indicate that at least a fraction of naturally transcribed intronic non-coding RNAs may be regulated by common physiological signals such as hormones, and further corroborate the notion that the intronic complement of the transcriptome play functional roles in the human gene-expression program

Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60mg/kg i.v., 10 days) and non diabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Pro-reitoria de Pesquisa da Universidade de Sao Paulo (PRP/USP, Projeto I and Novos Docentes)Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Lab Immunoendocrinol,FCF, Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Med, Rheumatol Div, Sao Paulo, SP, BrazilDepartment of Medicine, Rheumatology Division, Universidade Federal de São Paulo, São Paulo, SP, BrazilFAPESP: 2010/02272-0FAPESP: 2012/23998-4FAPESP: 2013/20904-1FAPESP: 2014/05214-1FAPESP: 2017/05100-4CNPq: 470523/2013-1CNPq: 301617/2016-3Web of Scienc

Studies on benthic communities of rocky shores on the Brazilian coast and climate change monitoring: status of knowledge and challenges

Um grupo de trabalho de costões rochosos (GT) integrado à ReBentos (Rede de Monitoramento de Habitats Bentônicos Costeiros), e vinculado à Sub-Rede Zonas Costeiras da Rede Clima (MCT) e ao Instituto Nacional de Ciência e Tecnologia para Mudanças Climáticas (INCT-MC), foi criado para estudar a vulnerabilidade das comunidades bentônicas dos costões rochosos e os efeitos das alterações ambientais sobre a respectiva biota. A presente síntese foi um dos produtos desse GT e teve como objetivo principal levantar e revisar o conhecimento existente sobre as comunidades bentônicas de costões rochosos na costa brasileira, a biodiversidade associada, e verificar as potencialidades de estudos futuros para uma previsão/mensuração mais acurada dos efeitos das mudanças climáticas sobre os ambientes e sua biota.A rocky shores working group (WG) integrated with ReBentos (Monitoring Network for Coastal Benthic Habitats; Rede de Monitoramento de Habitats Bentônicos Costeiros) was created and linked to the Coastal Zones Sub Network of the Climate Network (MCT; Sub-Rede Zonas Costeiras da Rede Clima) and to the National Institute of Science and Technology for Climate Change (INCT-MC; Instituto Nacional de Ciência e Tecnologia para Mudanças Climáticas), to study the vulnerability of benthic communities on rocky shores and the effects of environmental changes on biomes in such environments along the Brazilian coast. The synthesis presented here was one of the products of this GT, and aimed to collect and review existing knowledge on benthic communities present on rocky shores of the Brazilian coast, their associated biodiversity, and the potential of future studies to accurately predict/measure the effects of climate change on such environments and their biota

Towards a Multisemiotic and Multimodal Editor

Mobile devices, such as tablets and smartphones, are increasingly present in the everyday life of young people. Despite such insertion, the use of those devices in scenarios such as m-learning is still a missed opportunity. In a previous work we verified that an editor that aggregates various kinds of media can help students in their studies. With that motivation in mind, we realized the Multimodal Editor – a useful tool to leverage the multimedia authorship. In this paper we describe our trajectory toward the Editor – requirements gathering, implementation, challenges, usage scenario and evaluation.Facultad de Informátic

Maternal Fibroblast Growth Factor 21 Levels Decrease during Early Pregnancy in Normotensive Pregnant Women but Are Higher in Preeclamptic Women—A Longitudinal Study

Fibroblast growth factor 21 (FGF-21) is an endocrine factor involved in glucose and lipid metabolism that exerts pleiotropic effects. The aim of this study was to investigate the serum FGF-21 profile in healthy and mild preeclamptic pregnant women at each trimester of pregnancy; (2) Methods: Serum FGF-21 levels were determined by ELISA in a nested case-control study within a longitudinal cohort study that included healthy (n = 54) and mild preeclamptic (n = 20) pregnant women, women at three months after delivery (n = 20) and eumenorrheic women during the menstrual cycle (n = 20); (3) Results: FGF-21 levels were significantly lower in the mid-luteal phase compared to the early follicular phase of the menstrual cycle in eumenorrheic women (p < 0.01). Maternal levels of FGF-21 were significantly lower in the first and second trimesters and peaked during the third trimester in healthy pregnant women (p < 0.01). Serum levels of FGF-21 in healthy pregnant were significantly lower in the first and second trimester of pregnancy compared with the follicular phase of the menstrual cycle and postpartum (p < 0.01). Serum FGF-21 levels were significantly higher in preeclamptic compared to healthy pregnant women during pregnancy (p < 0.01); (4) Conclusions: These results suggest that a peak of FGF-21 towards the end of pregnancy in healthy pregnancy and higher levels in preeclamptic women might play a critical role that contributes to protecting against the negatives effects of high concentrations of non-esterified fatty acids (NEFA) and hypertensive disorder. Furthermore, FGF-21 might play an important role in reproductive function in healthy eumenorrheic women during the menstrual cycle