Efecto de la fertilización nitrogenada y la edad del rebrote sobre el valor nutritivo de Brachiaria brizantha cv. "Marandú"

In Brachiaria brizantha cv. Marandú, it was evaluated the nutritional value. Nutritional value was considered: crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and in vitro digestibility of dry matter (IVDDM). The variables were evaluated for age of regrowth (28, 56, 84 and 112 days) and fertilization (F0, F1, F2, F3 and F4). We determined the nutritional value. CP content showed interaction between age and fertilization. Nitrogen fertilization can increase the proportion of PB. The average values of regrowth age were 46, 48, 60, 71, and 77 g CP kg DM-1 to F1, F2, F3, F4 and F5 respectively. It was a positive answer to fertilization (627-645 g NDF kg DM-1 to F1 and F4) and age (567-683 g kg DM-1 from 28 to 112 days). In turn, fertilization had no effect on the ADF and if there was a positive response from the age of regrowth, with average values of 278 to 399 g kg DM-1 ADF at 28 and 112 days respectively. The IVDDM was affected by the age ranging from 603 to 370 g kg DM-1 from 28 days to 112 days. Age regrowth is the most important factor in the nutritional value.Se evaluó en Brachiaria brizantha cv Marandú, el valor nutritivo en función de la edad del rebrote (28, 56, 84 y 112 días) y fertilización (F1, F2, F3, F4 y F5). Se determinó proteína bruta (PB), fibra detergente neutro (FDN), fibra detergente ácido (FDA) y digestibilidad in vitro de la materia seca (DIVMS). El contenido de PB, mostró interacción entre edad de rebrote y fertilización. Los valores promedio por edad fueron de 46; 48; 60; 71; y 77 g PB Kg MS-1 para F1, F2, F3, F4 y F5 respectivamente. La fertilización nitrogenada permitió incrementar la proporción de PB. Sobre FDN hubo una respuesta positiva de la fertilización (627 a 645 g FDN Kg MS-1 para F1 y F5) y de la edad (567 a 683 g Kg MS-1 de los 28 a los 112 días) y sobre FDA hubo diferencias significativas en función de la edad, (con valores de 278 a 399 g FDA Kg MS-1 a los 28 y 112 días respectivamente). La DIVMS presentó diferencias significativas en función de la edad de rebrote variando de 603 a 370 g Kg MS-1 desde los 28 días a 112 días. En los parámetros analizados la edad de rebrote fue un factor determinante en el valor nutritivo

Tratamiento multimodal de contextos

We take as starting point the context logic studied in (Grossi, Meyer, and Dignum 2008) and (Aucher et al. 2009), which was concerned with the development of a dynamic logic of rules. Here we focus on issues of Formal Epistemology and omit the addition of deontic operators.We define some basic logics that are useful for the study of epistemic actions such as expanding, contracting or revising a theory taking into account different kinds of inferential results

Prospects for practice-based philosophy of logic

We explore prospects for practice-based approaches to logical theory, in particular the link between classical and intuitionistic logic and the inferential structure of traditional practices of representation & argument in science and mathematics. After discussing some key notions about practice, we outline the connection between representation practices and classical logic, and then consider a spectrum of actual practices followed or proposed by (real) scientists. Intuitionistic logic helps to clarify the potential of practice-based approaches for understanding pluralism, and to hammer some key points about the general thesis.Ministerio de Economía y Competitividad FFI2009-1002

Newborn screening for presymptomatic diagnosis of complement and phagocyte deficiencies

The clinical outcomes of primary immunodeficiencies (PIDs) are greatly improved by accurate diagnosis early in life. However, it is not common to consider PIDs before the manifestation of severe clinical symptoms. Including PIDs in the nation-wide newborn screening programs will potentially improve survival and provide better disease management and preventive care in PID patients. This calls for the detection of disease biomarkers in blood and the use of dried blood spot samples, which is a part of routine newborn screening programs worldwide. Here, we developed a newborn screening method based on multiplex protein profiling for parallel diagnosis of 22 innate immunodeficiencies affecting the complement system and respiratory burst function in phagocytosis. The proposed method uses a small fraction of eluted blood from dried blood spots and is applicable for population-scale performance. The diagnosis method is validated through a retrospective screening of immunodeficient patient samples. This diagnostic approach can pave the way for an earlier, more comprehensive and accurate diagnosis of complement and phagocytic disorders, which ultimately lead to a healthy and active life for the PID patientsThis work was supported by the Swedish Research Council (VR) and grants provided by the Stockholm County Council (ALF)

Adjuvant therapy sparing in rectal cancer achieving complete response after chemoradiation

AIM: To evaluate the long-term results of conventional chemoradiotherapy and laparoscopic mesorectal excision in rectal adenocarcinoma patients without adjuvant therapy. METHODS: Patients with biopsy-proven adenocarcinoma of the rectum staged cT3-T4 by endoscopic ultrasound or magnetic resonance imaging received neoadjuvant continuous infusion of 5-fluorouracil for five weeks and concomitant radiotherapy. Laparoscopic surgery was planned after 5-8 wk. Patients diagnosed with ypT0N0 stage cancer were not treated with adjuvant therapy according to the protocol. Patients with ypT1-2N0 or ypT3-4 or N+ were offered 5-fluorouracil-based adjuvant treatment on an individual basis. An external cohort was used as a reference for the findings. RESULTS: One hundred and seventy six patients were treated with induction chemoradiotherapy and 170 underwent total mesorectal excision. Cancer staging of ypT0N0 was achieved in 26/170 (15.3%) patients. After a median follow-up of 58.3 mo, patients with ypT0N0 had five-year disease-free and overall survival rates of 96% (95%CI: 77-99) and 100%, respectively. We provide evidence about the natural history of patients with localized rectal cancer achieving a complete response after preoperative chemoradiation. The inherent good prognosis of these patients will have implications for clinical trial design and care of patients. CONCLUSION: Withholding adjuvant chemotherapy after complete response following standard neoadjuvant chemoradiotherapy and laparoscopic mesorectal excision might be safe within an experienced multidisciplinary team

Regulation of cell death receptor S-nitrosylation and apoptotic signaling by Sorafenib in hepatoblastoma cells

Nitric oxide (NO) plays a relevant role during cell death regulation in tumor cells. The overexpression of nitric oxide synthase type III (NOS-3) induces oxidative and nitrosative stress, p53 and cell death receptor expression and apoptosis in hepatoblastoma cells. S-nitrosylation of cell death receptor modulates apoptosis. Sorafenib is the unique recommended molecular-targeted drug for the treatment of patients with advanced hepatocellular carcinoma. The present study was addressed to elucidate the potential role of NO during Sorafenib-induced cell death in HepG2 cells. We determined the intra- and extracellular NO concentration, cell death receptor expression and their S-nitrosylation modifications, and apoptotic signaling in Sorafenib-treated HepG2 cells. The effect of NO donors on above parameters has also been determined. Sorafenib induced apoptosis in HepG2 cells. However, low concentration of the drug (10nM) increased cell death receptor expression, as well as caspase-8 and -9 activation, but without activation of downstream apoptotic markers. In contrast, Sorafenib (10 µM) reduced upstream apoptotic parameters but increased caspase-3 activation and DNA fragmentation in HepG2 cells. The shift of cell death signaling pathway was associated with a reduction of S-nitrosylation of cell death receptors in Sorafenib-treated cells. The administration of NO donors increased S-nitrosylation of cell death receptors and overall induction of cell death markers in control and Sorafenib-treated cells. In conclusion, Sorafenib induced alteration of cell death receptor S-nitrosylation status which may have a relevant repercussion on cell death signaling in hepatoblastoma cells.Instituto de Salud Carlos III PI13/00021Ministerio de Economía y Competitividad BFU2012-32056Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía BIO-0216Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía CTS-6264Consejería de Salud, Junta de Andalucía PI13/ 0002

Abducción y semántica de teoría de juegos

An agency-inspired inferential game is proposed in which two agents (abductive and deductive) interact. It depicts hybrid inferencial contexts where deductive and abductive steps are mixed. The abductive agent can use results obtained by the deductive one but not vice versa due to the abductive process do not always generate deductive valid formulas.

Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity

Acknowledgements This work was supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01- 0145-FEDER-000013), the Fundação para a Ciência e Tecnologia (FCT) (SFRH/BD/136814/2018 to S.M.G., SFRH/BD/141127/2018 to C.D.O., PD/BD/137680/2018 to D.A., IF/00474/2014 to N.S.O., IF/01390/2014 to E.T., IF/00959/2014 to S.C., IF/00021/2014 to R.S., PTDC/SAU-SER/29635/2017 and CEECIND/04601/2017 to C.C., and CEECIND/03628/2017 to A.C.), the Institut Mérieux (Mérieux Research Grant 2017 to C.C.), and the European Society of Clinical Microbiology and Infectious Diseases (ESCMID Research Grant 2017 to A.C.). M.G.N. was supported by a Spinoza grant of the Netherlands Organization for Scientific Research. A.A.B. was supported by the Deutsche Forschungsgemeinschaft Collaborative Research Center/Transregio TR124 FungiNet (project A1). G.D.B. was funded by the Wellcome Trust (102705), the MRC Centre for Medical Mycology and the University of Aberdeen (MR/N006364/1).Peer reviewedPublisher PD

HTLV-1 infection in solid organ transplant donors and recipients in Spain

HTLV-1 infection is a neglected disease, despite infecting 10-15 million people worldwide and severe illnesses develop in 10% of carriers lifelong. Acknowledging a greater risk for developing HTLV-1 associated illnesses due to immunosuppression, screening is being widely considered in the transplantation setting. Herein, we report the experience with universal HTLV testing of donors and recipients of solid organ transplants in a survey conducted in Spain. All hospitals belonging to the Spanish HTLV network were invited to participate in the study. Briefly, HTLV antibody screening was performed retrospectively in all specimens collected from solid organ donors and recipients attended since the year 2008. A total of 5751 individuals were tested for HTLV antibodies at 8 sites. Donors represented 2312 (42.2%), of whom 17 (0.3%) were living kidney donors. The remaining 3439 (59.8%) were recipients. Spaniards represented nearly 80%. Overall, 9 individuals (0.16%) were initially reactive for HTLV antibodies. Six were donors and 3 were recipients. Using confirmatory tests, HTLV-1 could be confirmed in only two donors, one Spaniard and another from Colombia. Both kidneys of the Spaniard were inadvertently transplanted. Subacute myelopathy developed within 1 year in one recipient. The second recipient seroconverted for HTLV-1 but the kidney had to be removed soon due to rejection. Immunosuppression was stopped and 3 years later the patient remains in dialysis but otherwise asymptomatic. The rate of HTLV-1 is low but not negligible in donors/recipients of solid organ transplants in Spain. Universal HTLV screening should be recommended in all donor and recipients of solid organ transplantation in Spain. Evidence is overwhelming for very high virus transmission and increased risk along with the rapid development of subacute myelopathy