Estudio observacional: análisis del apoyo social percibido y la percepción subjetiva de recuperación en pacientes con trastorno mental grave

Treball final de Màster Universitari en Rehabilitació Psicosocial en Salut Mental Comunitària. Codi: SAX014. Curs acadèmic 2014-2015Objetivo: Comparar la Escala MARS y la Escala de Apoyo Social Percibido, MSPSS, en el paciente con TMG. Método: Estudio observacional de una muestra de 35 pacientes diagnosticados de TMG en el Hospital Psiquiátrico Román Alberca y Hospital Sta. Lucía entre marzo y junio de 2015. Las variables dependientes fueron el estado de recuperación subjetiva en personas con enfermedad mental grave cuantificada con la escala MARS (Maryland Assessment Recovery Scale) y como variable independiente el Apoyo Social Percibido, cuantificado con la escala MSPSS. Para valorar la relación entre variables, se comparó la distribución de porcentajes de la variables sociodemográficas y por otro lado la variable dependiente con la variable independiente. Para determinar si las diferencias eran estadísticamente significativas se utilizó el cociente de correlación de Pearson.Objective: To compare the MARS Scale and the Scale of Perceived Social Support, MSPSS, in patients with TMG. Method: Observational study of a sample of 35 patients diagnosed with TMG in the Roman Pool Psychiatric Hospital and St. Lucia Hospital between March and June 2015. The dependent variables were the state of subjective recovery in people with severe mental illness quantified with MARS (Maryland Recovery Assessment Scale) as the independent variable and the Perceived Social Support, MSPSS quantified the scale. To assess the relationship between variables, the percentage distribution of sociodemographic variables and on the other hand dependent variable with the independent variable it was compared. To determine whether the differences were statistically significant ratio Pearson correlation was used

Identification of polymers in waste tyre reinforcing fibre by thermal analysis and pyrolysis

The composition of the reinforcing fibre obtained from the recycling of scrap tyres was analyzed with a view to finding the most suitable applications for it. The material (RF) was separated into two different parts i.e. fibre (F) and microfibre (MF) to ensure the maximum homogeneity of the material under study. Thermogravimetric analysis (TG) together with differential scanning calorimetry (DSC) and infrared spectroscopy (FTIR) were used to identify the polymers present in the waste and to determine in what proportions they were present. Determination of the temperatures of maximum volatile matter evolution (Tmax), the yield at the end of the pyrolysis and the melting temperatures yielded very useful results for the identification of the polymers. In order to confirm the results obtained, pyrolysis of the pure polymers was carried out and the decomposition products were analyzed. The results were then compared with the oil composition data derived from the pyrolysis of the waste fibres. Our results showed the presence of two polyamides, rayon, polyester and aramid.The research leading to these results has received funding from theSpanish MICINN Project reference CTM2009-10227. BA thanks the Government of the Principado de Asturias for the award of a predoctoral grant with funds from PCTI-AsturiasPeer reviewe

Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples

Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU·μL−1. This method constitutes an innovative approach that could be potentially used at point of care for rapid mass screening of viral infectious diseases and monitoring of the SARS-CoV-2 pandemic.Instituto de Salud Carlos III COV20-00080 and COV20-00173Ministerio de Ciencia e Innovación EQC2019-006240-PComisión Europea JRC HUMAINT projec

Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept

Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.Ministerio de Ciencia e Innovación, 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease Instituto de Salud Carlos III by Grant Number EQC2019-006240-PComisión Europea, Joint Research Center (JRC) HUMAINT project

Podoplanin associates with CD44 to promote directional cell migration

This article is under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.-- et al.Podoplanin is a transmembrane glycoprotein up-regulated in different human tumors, especially those derived from squamous stratified epithelia (SCCs). Its expression in tumor cells is linked to increased cell migration and invasiveness; however, the mechanisms underlying this process remain poorly understood. Here we report that CD44, the major hyaluronan (HA) receptor, is a novel partner for podoplanin. Expression of the CD44 standard isoform (CD44s) is coordinately up-regulated together with that of podoplanin during progression to highly aggressive SCCs in a mouse skin model of carcinogenesis, and during epithelial-mesenchymal transition (EMT). In carcinoma cells, CD44 and podoplanin colocalize at cell surface protrusions. Moreover, CD44 recruitment promoted by HA-coated beads or cross-linking with a specific CD44 antibody induced corecruitment of podoplanin. Podoplanin-CD44s interaction was demonstrated both by coimmunoprecipitation experiments and, in vivo, by fluorescence resonance energy transfer/fluorescence lifetime imaging microscopy (FRET/FLIM), the later confirming its association on the plasma membrane of cells with a migratory phenotype. Importantly, we also show that podoplanin promotes directional persistence of motility in epithelial cells, a feature that requires CD44, and that both molecules cooperate to promote directional migration in SCC cells. Our results support a role for CD44-podoplanin interaction in driving tumor cell migration during malignancy.This work was supported by grant SAF2007-63821 from the Spanish Ministry of Science and Innovation (to M.Q.), the Royal Society University Research Fellowship (to M.P.), Medical Research Council (to G.E.J.) EU FP7 T3Net Consortium (GEJ), and Cancer Research UK (to G.E.J. and E.M.V.).Peer reviewe

Moxibustión en la malposición fetal

Introducción: La incidencia de la presentación fetal podálica en España es del 3,8%. La moxibustión es parte integral de la milenaria medicina tradicional china. La técnica consiste en la colocación de un palillo incandescente de Artemisia vulgaris a unos pocos centímetros del punto de acupuntura Zhiyin (punto 67 del meridiano de vejiga, ubicado en la base externa de la uña del quinto dedo del pie). Los objetivos son: 1. Conocer el porcentaje de fetos que han rotado a cefálica mediante la moxibustión en gestantes que presenten una malposición fetal a partir de las 32 semanas de embarazo; 2. Identificar las complicaciones materno-fetales en la aplicación de la técnica. Sujetos: Se estudiaron 18 gestantes de más de 32 semanas de embarazo. Material y métodos: Mediante un estudio descriptivo de intervención, analizamos el porcentaje de fetos que rotaron a presentación cefálica. Resultados: Quince gestantes (83,9%) realizaron el tratamiento adecuadamente y las 3 restantes (16,7%) lo realizaron de manera ocasional y terminaron con una cesárea electiva por nalgas. Conclusiones: Parece razonable concluir que el cumplimiento del tratamiento influye en el tipo de parto (χ2= 12,600; gl= 1; p= 0,000), aunque no modifica el Apgar del recién nacido. Esta técnica se presenta como una alternativa económica, segura, sencilla y práctica para la versión fetal de la presentación podálica.Introduction: The incidence of fetal breech presentation in Spain is of 3.8%. Moxibustion is an integral part of the ancient traditional Chinese medicine. The technique involves placing an incandescent stick of Artemisia vulgaris a few inches of the acupuncture point Zhiyin (point 67 of the bladder meridian, located at the outer base of the nail of the fifth toe). The objectives are: 1. To find out what percentage of fetuses have turned around to a cephalic presentation due to moxibustion in pregnant women displaying fetal malposition after the 32nd week of pregnancy; 2. To identify any materno-fetal complications associated with the use of the technique. Subjects: A total of eighteen pregnant women were studied with more than 32 weeks of gestation. Material and methods: Through a descriptive study with one group intervention, we analyzed the percentage of cephalic fetus that rotated with this therapy. Results: Fifteen pregnant women (83.9%) did the treatment properly; three pregnant women (16.7%) carried out the treatment on an occasional basis and ended with a caesarean section due to buttocks. Conclusions: It seems reasonable to say that compliance with the treatment influences the type of delivery (χ2= 12.600; gl= 1; p= 0.000), but does not modify the Apgar of the newborn. This technique is presented as an economic, safe, simple and practical alternative, for the fetal version of breech presentation

Prostaglandin transporter PGT as a new pharmacological target in the prevention of inflammatory cytokine-induced injury in renal proximal tubular HK-2 cells

Aims: Inflammatory cytokines contribute to proximal tubular cell (PTC) injury leading to the deterioration of renal function and acute kidney injury (AKI) development. They also stimulate cyclo‑oxygenase-2 (COX-2)- dependent production and release to the extracellular medium of prostaglandin E2 (PGE2), a mediator of PTC injury. However, in several settings PGE2 re-uptake by prostaglandin transporter (PGT) is critical for PGE2- mediated PTC injury. Here we investigated several deleterious effects of pro-inflammatory cytokines in PTC and their prevention by PGT targeting. Main methods: In human kidney-2 (HK-2) PTC exposed to an inflammatory cytokine cocktail, consisting of interleukins (IL) IL-1α, IL-1β and IL-2, tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), were determined the changes in several parameters related to PTC injury, their dependency on PGE2 (through modulation by antagonists of PGE2 receptors) and the preventive effect of PGT inhibitor bromosulfophthalein. Key findings: The cytokine cocktail induced a COX-2-dependent increase in intracellular PGE2 (iPGE2) and cell death, together to a decrease in cell number and cell proliferation. There was also loss of adherent cells to collagen IV, changes in actin cytoskeleton and loss of monolayer integrity, together to an increase in paracellular permeability. All the changes were sensitive to antagonist of PGE2 receptors AH6809 and were fully prevented by bromosulfophthalein. Significance: These results indicate that PGT-, iPGE2-dependent mechanisms mediate inflammatory cytokineinduced HK-2 cell injury and suggest that treatment with PGT inhibitors might help to prevent AKI induced by sepsis, renal ischemia/reperfusion and other pathological conditions in which inflammatory cytokines contribute to PTC damageThis work was supported by a grant COVID-19 2021 2020/00003/ 016/001/009 from the Universidad de Alcala and a grant Ayudas a la Investigacion ´ Departamento de Biología UAM. This research is part of the project on COVID-19 and diabetes (REACT UE-CM2021-02) funded by the Community of Madrid in agreement with the University of Alcala, ´ and co-funded with REACT-EU resources from the European Regional Development Fund “A way to make Europe

Study of the crystallization in fast sintered Na-rich plagioclase glass-ceramic

A dense, ∼94% of crystalline phase, glass-ceramic based on Na-rich plagioclase feldspar crystallizations has been obtained by a fast sintering process for the first time. By using Sr2+ and Ca2+ as nucleating agents, a unique hierarchical micro-nanostructure is developed in a fast sintering process at 1220 °C. In this work, a thorough study about crystallization processes of this novel material has been extensively carried out through thermal quenching experiments and supported by DTA/TG, Hot stage microscopy, XRD, FE-SEM, TEM/HRTEM and XPS measurements. The study reveals that crystallization takes place in two steps during the sintering process. Microcrystals nucleate on heating at temperatures >900 °C, while nanocrystals start nucleating at the dwelling temperature, 1220 °C. However, it is during holding and cooling time when nanostructures zones grow, and the unique highly crystalline micro-nanostructure is developed. Structural and compositional analysis supported by NMR, shows the main presence of Q4(nAl) albite-like sites and in lesser extent anorthite-like sites, corroborating the formation of a sodic plagioclase structure. The analysis of Si neighbours indicates that chemical environment presents a slight Si/Al disorder at short range.The authors express their thanks to the MINECO project MAT2017- 86450-C4-1-R, and project CDTI of CTV for their financial support. Dra. E. Enriquez is also indebted to MINECO for a ‘‘Torres Quevedo’’ contract (ref: PTQ-14-07289), which is co-financed with European Social Funds

Narrativas, Mindfulness y diálogo colaborativo

En las últimas décadas asistimos a un interés creciente por la integración de Mindfulness en psicoterapia. En este texto se revisan brevemente los resultados de los programas más difundidos, que, desde diferentes escuelas, han incluido Mindfulness como un factor clave para el cambio. En base a las pocas diferencias de resultado que los diferentes programas psicoterapéuticos tienen entre sí, se ofrece una reflexión sobre las áreas de convergencia entre Mindfulness y las corrientes narrativas como marco potencial para los factores comunes y la integración en psicoterapia.In recent decades we have witnessed a growing interest in the integration of Mindfulness in psychotherapy. This text briefly reviews the results of the most widespread programs, which, from different schools, have included Mindfulness as a key factor for change. Based on the few differences in the results of the different psychotherapeutic programs, a reflection is offered on the areas of convergence between Mindfulness and narrative currents as a potential framework for common factors and integration in psychotherap

Iron(iii) oxamato-catalyzed epoxidation of alkenes by dioxygen and pivalaldehyde

A new iron(III)–carbonato monomeric complex of orthophenylenebis( oxamato) (opba) 1 is synthesized, and spectroscopically and structurally characterized; it is a moderately efficient non-heme catalyst for the aerobic epoxidation of alkenes with co-oxidation of pivalaldehyde.Ruiz Garcia, Rafael, [email protected] ; Fernandez Picot, Isabel, [email protected] ; Pedro Llinares, Jose Ramon, [email protected]