Multi-contact epineural electrical stimulation to restore upper-limb functions

International audienceWe investigated the feasibility of a novel approach aiming at restoring functional movements in completely paralyzed upper limbs using neural stimulation. Two multi-contact cuff electrodes were wrapped around radial and median nerves, unilaterally, in 2 individuals with complete high tetraplegia. Electrodes were maintained for 28 days. Advanced stimulation configurations were implemented to achieve selective activation of fascicles and elicit different graspings. A control interface was developed to allow users to pilot movements of their hand by triggering stimulation. Both participants were able to execute palmar and key pinch grasping during functional tasks

Coastal monitoring solutions of the geomorphological response of beach-dune systems using multi-temporal LiDAR datasets (Vendée coast, France)

International audienceThree beach and dune systems located in the northeastern part of the Bay of Biscay in France were monitored over 5 years with a time series of three airborne LiDAR datasets. The three study sites illustrate a variety of morphological beach types found in this region. Reproducible monitoring solutions adapted to basic and complex beach and dune morphologies using LiDAR time series were investigated over two periods bounded by the three surveys. The first period (between May 2008 and August 2010) is characterized by a higher prevalence of storm events, and thus has a greater potential for eroding the coast, than the second period (between August 2010 and September 2013). During the first period, the central and northeastern part of the Bay of Biscay was notably impacted by Storm Xynthia, with water levels and wave heights exceeding the 10-year return period and 1-year return period, respectively. Despite differences in dune morphology between the sites, the dune crest (Dhigh) and the dune base (Dlow) are efficiently extracted from each DEM. Based on the extracted dune base, an original shoreline mobility indicator is built displaying a combination of the horizontal and vertical migrations of this geomorphic indicator between two LiDAR datasets. A 'Geomorphic Change Detection' is also completed by computing DEMs of Difference (DoD) resulting in segregated maps of erosion and deposition and sediment budgets. Accounting for the accuracy of LiDAR datasets, a probabilistic approach at a 95% confidence interval is used as a threshold for the Geomorphic Change Detection showing more reliable results. However, caution should be taken when interpreting thresholded maps of changes and sediment budgets because some beach processes may be masked, especially on wide tidal beaches, by only keeping the most significant changes. The results of the shoreline mobility and Geomorphic Change Detection show a high variability in the beach responses between and within the three study sites, explained mainly by beach orientation and local factors. Despite variable site-specific mechanisms, the recovery processes redistribute the available sand more on the upper parts of the beach, producing significant deposition generally in the form of embryo dunes. The monitoring of the beach and dune systems with airborne LiDAR datasets reveals that the three study sites show diverse behaviours during the first period likely associated with storms, while the analysis show more homogenous beach responses during the second period likely associated with a recovery phase

AGILIS: Restoring functional grasping in individuals with tetraplegy using epineural electrodes

International audienceWe propose a novel approach to restore grasping in individuals with complete tetraplegia using epineural stimulation. Two multi-contact cuff electrodes were positioned around radial and median nerves in 2 volunteers during a surgery. The electrodes were maintained for 28 days. A user interface allowed the triggering of pre-programmed stimulation sequences on demand by executing stereotyped movements or by contracting voluntarily muscle on the contralateral shoulder. The stimulation selectivity obtained with the epineural electrodes was sufficient to obtain functional palmar and key pinch grip

Activating effective functional hand movements in individuals with complete tetraplegia through neural stimulation

International audienceAbstract Individuals with complete cervical spinal cord injury suffer from a permanent paralysis of upper limbs which prevents them from achieving most of the activities of daily living. We developed a neuroprosthetic solution to restore hand motor function. Electrical stimulation of the radial and median nerves by means of two epineural electrodes enabled functional movements of paralyzed hands. We demonstrated in two participants with complete tetraplegia that selective stimulation of nerve fascicles by means of optimized spreading of the current over the active contacts of the multicontact epineural electrodes induced functional and powerful grasping movements which remained stable over the 28 days of implantation. We also showed that participants were able to trigger the activation of movements of their paralyzed limb using an intuitive interface controlled by voluntary actions and that they were able to perform useful functional movements such as holding a can and drinking through a straw

Oligonucleotide-polyethylenimine complexes targeting retinal cells: structural analysis and application to anti-TGFbeta-2 therapy.

PURPOSE: The aim of this study was to characterize oligonucleotide-polyethylenimine (ODN/PEI) complex preparation for potential transfection of retinal cells in vitro and in vivo. METHODS: The effect of medium preparation [HEPES-buffered saline (HBS), water] on particle size and morphology was evaluated. Cultured Lewis rat retinal Müller glial (RMG) cells were transfected using fluorescein isothiocyanate (FITC)-ODN/PEI complexes specifically directed at transforming growth factor beta (TGFbeta)-2. Efficacy of transfection was evaluated using confocal microscopy, and regulation of gene expression was assayed using quantitative real-time RT-PCR and ELISA assay. One, 24, and 72 h after injection of FITC-ODN/PEI complexes into the vitreous of rat eyes, their distribution was analyzed on eye sections. RESULTS: Complexes prepared in HBS were smaller than complexes prepared in pure water and presented a core-shell structure. These particles showed a high cellular internalization efficacy, along with a significant and specific down-regulation of TGFbeta-2 expression and production in RMG cells, correlating with specific inhibition of cell growth at 72 h. In vivo, complexes efficiently transfect retinal cells and follow a transretinal migration at 24 h. After 72 h, ODN seems to preferentially target RMG cells without inducing any detectable toxicity. CONCLUSIONS: Specific down-regulation of TGFbeta-2 expression using ODN/PEI complexes may have potential interest for the treatment of retinal diseases associated with glial proliferation

MACVIA-LR (Fighting Chronic Diseases for Active and Healthy Ageing in Languedoc-Roussillon): A Success Story of the European Innovation Partnership on Active and Healthy Ageing.

International audienceThe Région Languedoc Roussillon is the umbrella organisation for an interconnected and integrated project on active and healthy ageing (AHA). It covers the 3 pillars of the European Innovation Partnership on Active and Healthy Ageing (EIP on AHA): (A) Prevention and health promotion, (B) Care and cure, (C) and (D) Active and independent living of elderly people. All sub-activities (poly-pharmacy, falls prevention initiative, prevention of frailty, chronic respiratory diseases, chronic diseases with multimorbidities, chronic infectious diseases, active and independent living and disability) have been included in MACVIA-LR which has a strong political commitment and involves all stakeholders (public, private, patients, policy makers) including CARSAT-LR and the Eurobiomed cluster. It is a Reference Site of the EIP on AHA. The framework of MACVIA-LR has the vision that the prevention and management of chronic diseases is essential for the promotion of AHA and for the reduction of handicap. The main objectives of MACVIA-LR are: (i) to develop innovative solutions for a network of Living labs in order to reduce avoidable hospitalisations and loss of autonomy while improving quality of life, (ii) to disseminate the innovation. The three years of MACVIA-LR activities are reported in this paper