Evaluation of Biomass and Vegetative Propagation of Spilanthes Oleracea Jacq. (Asteraceae)

Spilanthes oleracea Jacq. is an herbaceous plant whose scientific literature attributes among others, anti-malarial and anti-bilharzia properties. These virtues justify the placing on the market of drugs based on the plant. Our study consisted on the one hand to evaluate the biomass of the plant on a soil of dune amended and on soil of unamended dune and to test its vegetative multiplication by transplanting, cuttings and layering. The results show that the growth of the species is greater on dune soil amended with an average biomass of 106.06 g compared to 71.06 g for un-amended soil plants. The transplanting of the plants and the layering were techniques that made it possible to multiply the plants. Spilanthes oleracea Jacq. can be produced using this agronomic data

Synthèse Et Caractérisation De La Structure Du [{4-[(1HBenzo[D]Imidazol-1-Yl)Méthyl]- 1H-1,2,3-Triazol-1 Yl}(Benzamido)Méthyl]Phospho nate De Diéthyle Par La Spectroscopie RMN 1D Et 2D

Le composé [{4-[(1H-benzo[d]imidazol1-yl)méthyl]-1H-1,2,3-triazol-1- yl}(benzamido) méthyl] phosphonate de diéthyle ester synthétisé de façon régiosélective, par une réaction de cycloaddition dipolaire -1,3 entre l’αazidoamino méthylphosphonate de diéthyle et le 1-(prop-2-yn-1-yl)-1Hbenzo[d]imidazole. La réaction de cyclisation par Chimie click est réalisée dans un mélange de solvant eau/éthanol (50/50) en présence de sulfate de cuivre pentahydraté et de l’ascorbate de sodium en quantité catalytique. Le dérivé bihétérocyclique de l’analogue phosphonique de la glycine est ainsi obtenu avec un excellent rendement (90%), sous forme de solide blanc après chromatographie sur colonne de gel de silice (acétate d’éthyle/hexane : 1/1) et recristallisation dans un mélange éther/hexane. La caractérisation de la structure attribuée de l’isomère -1,4, a été réalisée par le biais de la spectroscopie RMN 1D et 2D ainsi que sur la base des données de la littérature concernant la réaction de cycloaddition par Chimie Click. La 2D Spectroscopie RMN a joué un rôle fondamentale grâce aux différentes corrélations observées entre les hydrogènes et les carbones adjacents et aussi entre les hydrogènes et les carbones lointains. The compound, diethyl [{4-[(1H-benzo[d]imidazol-1-yl)methyl]-1H1,2,3-triazol-1-yl} (benzamido)methyl]phosphonate, was prepared in a regioselective manner, through 1,3-dipolar cycloaddition reaction between αazidoaminomethyl phosphonate and 1-(prop-2-yn-1-yl)-1Hbenzo[d]imidazole. The cyclization reaction by click chemistry was carried out in a water/ethanol solvent mixture (50/50), in the presence of copper sulfate pentahydrate and sodium ascorbate in catalytic quantities. The biheterocyclic derivative of the phosphonic analog of glycine was obtained with an excellent yield (90%), as a white solid after chromatography on a silica gel column (ethyl acetate/hexane: 1/1) and recrystallization in an ether/hexane mixture. The characterization of the structure of the resulting 1,4-isomer was performed by 1D and 2D NMR spectroscopy as well as on the basis of data from the literature concerning the cycloaddition reaction by Chemistry Click. The 2D NMR Spectroscopy played a fundamental role through different correlations observed between the hydrogens and adjacent carbons and also between hydrogens and distant carbons

Validation of an UV-Visible spectrophotometry assay method for the determination of chlorpheniramine maleate tablets without prior extraction

Methods proposed by pharmacopeias to check the quality of chlorpheniramine maleate tablets are multi steps methods which involve extraction and present issues with repeatability. An alternative method is proposed with a sensitive, reliable, simple and rapid UV-VISIBLE spectrophotometry method developed for the determination without extraction of chlorpheniramine maleate in tablets. The method was validated using the accuracy profile approach with an accuracy ranging from 99.70 to 100.46%. Analysis was done using 0.25 mol/L sulfuric acid, distilled water, and raw material in a room at 23 °C for 25 to 30 minutes. Chlorpheniramine maleate concentration varied from 0.018 to 0.03 mg/mL. The method was found to be specific with the appearance of the corresponding maxima at 265 nm and a correlation coefficient (R2 ) of 0.9993. Limits of detection and quantification were respectively 1.39 × 10-4and 2.26 × 10-3 mg/mL. The tolerance limits for the different concentration levels (75, 100 and 125%) were respectively: 88.37; 92.74 and91.62% for lower limits and 111.03; 108.17 and 108.02% for upper limits. It was observed that the tolerance limits were within the limits of acceptability set at 20%. Consequently, the method was declared valid and reliable for routine analysis of chlorpheniramine maleate in tablets containing chlorphenamine as active principle.Keywords : Chlorpheniramine maleate, tablets, accuracy profile, assay method without extraction, validatio

Functionalization of amorphous nitrogenated carbon thin film electrodes for improved detection of cadmium vs. copper cations

International audienceThe specificity of a sensor relative to specific cations can be modified by functionalization of amorphous nitrogenated carbon thin film (a-C:Nx) electrodes. This surface modification was performed through the reduction of diazonium salts presenting different functionalities which could interact selectively with ions in solution. Cyclic voltammetry from +0.3 to −1.0 V vs. SCE was used for the grafting process leading to the formation of covalent CC bonds onto the film surface. The precursor concentration and number of cycles were optimized and correlated to the specificity and sensitivity for copper and/or cadmium ion detection. The coverage of the electrode was checked by cyclic voltammetry measurements using the Fe(CN)63−/4− system, interface charge transfer and contact angle. It showed that the electroreduction of diazonium salt and grafting of substituted aryl groups was effective on the surface of the a-C:Nx electrodes. A greater sensitivity of the electrode to Cd2+ was observed for the highest concentrations of precursors (4-amino benzene carboxylic acid (4-ABA)) compared to the pristine electrodes. Moreover, a marked decrease of cadmium sensitivity is noted after grafting but at the same time with a decreased influence of copper on the Cd2+ signal, indicating a better specificity of these functionalized sensor

Anti-Inflammatulatory Activity of Hydro-Ethanolic Bark Extracts of Piliostigma reticulatum Hochst (Caesalpiniaceae) and Analysis by HPLC-DAD and HPLC-MS of its Methanol Fraction

Use of plants as remedies dates back to the time of the oldest civilizations. Among these active ingredients, polyphenols play an important role. Piliostigma reticulatum, a plant whose barks are rich in condensed tannin is often used as an anti-inflammatory. The purpose of this work was to correlate the anti-inflammatory activity of extracts with their chemical composition through chromatography fractionation analyses (HPLC-DAD and HPLC-MS). The barks were extracted by moderate hydroethanol decoction followed by silica gel splitting with successively ethyl acetate, methanol and water. Anti-inflammatory activity was evaluated by the method of carrageenan induced hind paw edema in Wistar rats.  The chemical study was carried out by HPLC-DAD and HPLC-MS after isolation of the compounds by fractionation on Sephadex LH-20 gel. The bark hydroethanolic extract had shown ability to inhibit significantly the inflammation induced by carraghenan. Among the hydroethanolic bark extract fractions that methanol fraction had the best activity at 10 mg/kg with inhibition percentages similar to those of acetylsalicylic acid with 48.51% ± 2.26; 54.71% ± 5.13; 64.19% ± 6.70 vs. 54.69% ± 7.23; 57.83% ± 9; 65.13% ± 8.44 respectively. The HPLC-DAD analysis showed that the SF5 sub-fraction presented the best chromatogram with several peaks, three of which were high intensity. The latter would correspond to monomer, dimer and trimer of catechin according to the weights [M-H] + m / z: 291.07; 579.18; 867.27 obtained by HPLC-MS. The compounds responsible for anti-inflammatory activity would be condensed tannins. The latter would consist mainly of catechin oligomers.  Keys words: Piliostigma reticulatum, bark, anti-inflammatory activity, HPLC-DAD, HPLC-M

Icacina senegalensis (Icacinaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity

<p>Abstract</p> <p>Background</p> <p>With the aim of discovering new natural active extracts against malaria parasites, <it>Icacina senegalensis </it>was selected after an ethnopharmacological survey conducted on plants used in traditional malaria treatment in Senegal.</p> <p>Methods</p> <p>Different concentrations of the plant extract and fractions were tested on synchronized <it>Plasmodium falciparum </it>cultures at the ring stage using the parasite lactate dehydrogenase assay. Their haemolytic activity and <it>in vitro </it>cytoxicity were evaluated. The chromatographic profiles of active fractions were also established.</p> <p>Results</p> <p>The plant extract and fractions revealed anti-plasmodial activity (IC₅₀< 5 μg/mL) with no toxicity (Selectivity indexes >10). The dichloromethane fraction showed stronger anti-plasmodial activity than the total extract.</p> <p>Conclusion</p> <p>Anti-plasmodial activity and toxicity of <it>I. senegalensis </it>are reported for the first time and showed promising results in malaria field research.</p

Evaluation de l’activité antioxydante des extraits des feuilles de Aphania senegalensis (Sapindaceae) et de Saba senegalensis (Apocynaceae)

Les plantes traditionnelles présentent généralement de nombreuses propriétés thérapeutiques. L’objectif de la présente étude consistait à évaluer l’activité antioxydante des extraits des feuilles de Aphania senegalensis et de Saba senegalensis par spectrophotométrie moléculaire au moyen des méthodes de piégeage des radicaux libres 2,2-diphényl-1-picryl-hydrazyle (DPPH•) et acide 2,2’-azino-bis-(3-éthylbenzothiazoline-6- sulfonique) (ABTS+•). Une extraction éthanolique des feuilles de ces deux plantes a été effectuée au Soxhlet. Les deux extraits secs, redissouts dans de l’eau, ont été fractionnés en utilisant successivement l’hexane, le dichlorométhane et l’acétate d’éthyle. Les propriétés antioxydantes des extraits et celles de leurs différentes fractions ont été évaluées à différentes concentrations : 5, 10, 25 et 150 μg/ml. Les pourcentages d’inhibition (PI) expriment l’effet antioxydant mesuré. Une activité de piégeage des deux radicaux libres a été associée aux deux extraits et à l’ensemble des fractions. Pour les tests d’inhibition de l’absorbance du radical DPPH•, les PI ont varié de (22,20±0,03)% à (91,30±0,08)%. Avec le radical ABTS+•, les PI ont varié de (54,37±0,02)% à (99,13±0,01)%. Les extraits éthanoliques des feuilles de Aphania senegalensis et de Saba senegalensis et leurs différentes fractions présentent ainsi un pouvoir antioxydant.© 2015 International Formulae Group. All rights reserved.Mots clés: Plante médicinale, ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6 sulfonic acid)), DPPH (2,2- diphényl-1-picryl-hydrazyle), spectrophotométrie moléculaireEnglish Title: Antioxidant activity of leaves extracts of Aphania senegalensis (Sapindaceae) and Saba senegalensis (Apocynaceae)English AbstractSeveral therapeutic properties are often associated with traditional plants. The antioxidant properties of Aphania senegalensis and Saba senegalensis leaf extracts were evaluated by molecular spectrophotometry and using two radical scavenging methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and 2,2’-azino bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The leaves of each plant were extracted with ethanol  using a Soxhlet extractor apparatus. The two dry ethanolic extracts were dissolved in water then fractionated using successively hexane, dichloromethane and ethyl acetate. The antioxidant activities of the extracts and their different fractions were determined at various concentrations: 5, 10, 25 and 150 μg/ml. The antioxidant capacity was expressed as percent inhibition (PI). The extracts and their different fractions scavenged DPPH• and ABTS+• free radicals. The DPPH assay showed PI varying from 22.20±0.03% to 91.30±0.08%. With the ABTS+• radical, the PI varied from 54.37±0.02% to 99.13±0.01%. The ethanolic extracts of Aphania senegalensis and Saba senegalensis as well as their fractions showed antioxidant capacities.© 2015 International Formulae Group. All rights reserved.Keywords: Medicinal plant, ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6 sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl), molecular spectrophotometr