52 research outputs found
Pulsed extraction of ionization from helium buffer gas
The migration of intense ionization created in helium buffer gas under the
influence of applied electric fields is considered. First the chemical
evolution of the ionization created by fast heavy-ion beams is described.
Straight forward estimates of the lifetimes for charge exchange indicate a
clear suppression of charge exchange during ion migration in low pressure
helium. Then self-consistent calculations of the migration of the ions in the
electric field of a gas-filled cell at the National Superconducting Cyclotron
Laboratory (NSCL) using a Particle-In-Cell computer code are presented. The
results of the calculations are compared to measurements of the extracted ion
current caused by beam pulses injected into the NSCL gas cell.Comment: Accepted for pubilication in Nucl. Instrum. Meth. B, 14 pages, 8
figure
Intensity limitations of a gas cell for stopping, storing and guiding of radioactive ions
The possibility to use a gas cell filled by noble gas (He or Ar) for thermalizing, storing and transporting radioactive ions is explored by studying experimentally ion - electron recombination of stable Ni, resonantly ionized by laser light. Combined with a literature study on ionization chambers, especially developed for high-intensity applications, conclusions are drawn on the maximum intensity of the incoming ion beam. A practical limit is encountered when the space-charge induced voltage fully counteract the applied voltage on the electrodes collecting the electrons
Shape Isomerism at N = 40: Discovery of a Proton Intruder in 67Co
The nuclear structure of 67Co has been investigated through 67Fe beta-decay.
The 67Fe isotopes were produced at the LISOL facility in proton-induced fission
of 238U and selected using resonant laser ionization combined with mass
separation. The application of a new correlation technique unambiguously
revealed a 496(33) ms isomeric state in 67Co at an unexpected low energy of 492
keV. A 67Co level scheme has been deduced. Proposed spin and parities suggest a
spherical (7/2-) 67Co ground state and a deformed first excited (1/2-) state at
492 keV, interpreted as a proton 1p-2h prolate intruder state.Comment: 4 pages, 5 figures, preprint submitted to Physical Review Letter
Dermatomyositis and Breast Calcinosis
The authors report a rare case of dermatomyositis diagnosed at the Mastology Sector of the Division of Gynecology of the Federal University of São Paulo - Escola Paulista de Medicina, which caused breast deformity due to formation of bilateral dystrophic calcifications.Os autores relatam caso raro de dermatomiosite diagnosticado no Setor de Mastologia da Disciplina de Ginecologia da Universidade Federal de São Paulo (UNIFESP) - Escola Paulista de Medicina, causando deformidade mamária devido à formação de calcificações distróficas bilateralmente.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Setor de MastologiaUNIFESP, EPM, Setor de MastologiaSciEL
Dual Chamber Laser Ion Source at Lisol
A new type of the gas cell for the resonance ionization laser ion source at
the Leuven Isotope Separator On Line (LISOL) has been developed and tested at
off-line and on-line conditions. Two-step selective laser ionization is applied
to produce purified beams of radioactive isotopes. The selectivity of the ion
source has been increased by more than one order of magnitude by separation of
the stopping and laser ionization regions. This allows to use electrical fields
for further ion purification.Comment: 14 figure
Furuncular myiasis of the breast caused by the larvae of the Tumbu fly (Cordylobia anthropophaga)
The Laser Ion Source Trap (LIST) coupled to a gas cell catcher
The proof of principle of the Laser Ion Source Trap (LIST) coupled to a gas
cell catcher system has been demonstrated at the Leuven Isotope Separator
On-Line (LISOL). The experiments were carried out by using the modified gas
cell-based laser ion source and the SextuPole Ion Guide (SPIG). Element
selective resonance laser ionization of neutral atoms was taking place inside
the cold jet expanding out of the gas cell catcher. The laser path was oriented
in longitudinal as well as transverse geometries with respect to the atoms
flow. The enhancement of beam purity and the feasibility for in-source laser
spectroscopy were investigated in off-line and on-line conditions.Comment: 11 pages, 13 figure
Cathepsin K induces platelet dysfunction and affects cell signaling in breast cancer - molecularly distinct behavior of cathepsin K in breast cancer
Background: Breast cancer comprises clinically and molecularly distinct tumor subgroups that differ in cell histology and biology and show divergent clinical phenotypes that impede phase III trials, such as those utilizing cathepsin K inhibitors. Here we correlate the epithelial-mesenchymal-like transition breast cancer cells and cathepsin K secretion with activation and aggregation of platelets. Cathepsin K is up-regulated in cancer cells that proteolyze extracellular matrix and contributes to invasiveness. Although proteolytically activated receptors (PARs) are activated by proteases, the direct interaction of cysteine cathepsins with PARs is poorly understood. In human platelets, PAR-1 and -4 are highly expressed, but PAR-3 shows low expression and unclear functions. Methods: Platelet aggregation was monitored by measuring changes in turbidity. Platelets were immunoblotted with anti-phospho and total p38, Src-Tyr-416, FAK-Tyr-397, and TGF beta monoclonal antibody. Activation was measured in a flow cytometer and calcium mobilization in a confocal microscope. Mammary epithelial cells were prepared from the primary breast cancer samples of 15 women with Luminal-B subtype to produce primary cells. Results: We demonstrate that platelets are aggregated by cathepsin K in a dose-dependent manner, but not by other cysteine cathepsins. PARs-3 and -4 were confirmed as the cathepsin K target by immunodetection and specific antagonists using a fibroblast cell line derived from PARs deficient mice. Moreover, through co-culture experiments, we show that platelets activated by cathepsin K mediated the up-regulation of SHH, PTHrP, OPN, and TGF beta in epithelial-mesenchymal-like cells from patients with Luminal B breast cancer. Conclusions: Cathepsin K induces platelet dysfunction and affects signaling in breast cancer cells.Associacao Beneficente de Coleta de Sangue (Colsan)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Univ Fed Sao Paulo, Dept Gynecol, BR-04024002 Sao Paulo, SP, BrazilCOLSAN, Charitable Assoc Blood Collect, BR-04080006 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biophys, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biochem, BR-04024002 Sao Paulo, SP, BrazilAntonio Prudente Fdn, AC Camargo Canc Ctr, AC Camargo Hosp Biobank, Dept Pathol, BR-01509010 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Cellular Gynecol Lab, Dept Gynecol, Rua Napoleao Barros 608, BR-04024002 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Gynecol, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biophys, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Dept Biochem, BR-04024002 Sao Paulo, SP, BrazilUniv Fed Sao Paulo, Cellular Gynecol Lab, Dept Gynecol, Rua Napoleao Barros 608, BR-04024002 Sao Paulo, BrazilFAPESP: 2012/19780-3FAPESP: 2012/19851-8FAPESP: 2009/53766-5Web of Scienc
Low birth weight at term and the presence of fine particulate matter and carbon monoxide in the Brazilian Amazon: a population-based retrospective cohort study
Cathepsin K induces platelet dysfunction and affects cell signaling in breast cancer - molecularly distinct behavior of cathepsin K in breast cancer
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