Changes in flavour influencing parameters of coldstored orange fruit

Orange fruits may undergo changes in sensory quality during prolonged cold storage, probably associated with decreased emission of those volatile compounds characterising aroma profile of fresh fruits, as well as with development of off-flavours. The purpose of this work was to evaluate modifications in aroma of two blood varieties (‘Tarocco’ and ‘Moro’) and a blond variety (cv. ‘Washington navel’) of orange fruit [Citrus sinensis (L.) Osbeck] after storage at 6 °C and 90-95 % RH during 60 days. Standard quality parameters (juice yield, TSS, TA, pH, vitamin C) in addition to anthocyanin content, volatile flavour compounds in fruit juice and biogenic amines) were determined during fruit storage. Moreover, the spoilage level was assessed by a sensory panel. GC-MS analysis showed the formation of detrimental volatile components such as p-vinylguaiacol and ethanol in blood varieties. In addition, oxidation products of limonene such as α-terpineol in both blood and blond varieties were identified. The predominant biogenic amine was putrescine in all cultivars. In fruit of the blood varieties its levels increased through storage time, the highest levels being present after 60 days of storage, while in fruit of the blond variety putrescine levels increased more slightly. These results are in accordance with those obtained by sensory evaluation that has shown an increase of off-flavours in the last period of storage for blood orange fruits

Innovative tools for nitrogen fertilization traceability in organic farming products: a cauliflower case study

Different research works have been carried out over the years to investigate new and reliable systems to test the authenticity of products obtained using organic cultivation methods. Based on a previously proposed integrated approach for discriminating organic from conventional products through the acquisition of isotopic data and other chemical and biochemical parameters, we herein report the results of an open-field cultivation case study for cauliflower crop. Experiments were carried out on soil, leaves, and corymb samples of cauliflowers grown using six different nitrogen fertilization treatments (organic, conventional, and mixed at different % of mineral fertilizers). The results of this study have shown that a multivariate analysis of isotopic data (13C/12C; 15N/14N, 34S/32S, 2H/1H, and 18O/16O isotopic ratios) combined with other parameters (fresh weight, total soluble solids, total acidity, cut resistance, CIE L*, a*, b* color indices, head height, head diameter, ascorbic acid content, total polyphenols, and ORAC units) performed using the linear discriminant analysis method gives researchers the possibility to discriminate organic products from conventional ones. Our study highlighted that the different isotopic signatures impressed on the cauliflowers by the different nitrogenous sources combined with the qualitative pattern of the crop, significantly affected by the different treatments, could effectively be jointly used to trace the organic origin of the cro

Total humerus replacement for osteosarcoma with proximal part of humerus: a case report

Incisional biopsy and intramedullary pinning were performed for pathological fracture associated with a malignant bone tumor of the proximal humerus. Osteosarcoma, for which preoperative chemotherapy had been performed, was confirmed by postoperative pathological examination. To achieve wide resection and acquire a safe resected margin, total humerus replacement was performed, and the whole humerus was reconstructed using the Howmedica Modular Reconstruction system. The patient resumed normal activities, although mild contracture of the elbow joint remains 8 years after surgery

Wholegrain Durum Wheat Bread Fortified With Citrus Fibers: Evaluation of Quality Parameters During Long Storage

The aim of this work was to evaluate the effect of the addition of citrus fibers, from blood orange and lemon peels to produce a functional durum wheat bread. Breads fortified in fiber were packaged under a modified atmosphere (MAP) and stored at 25°C up to 120 days. No significant differences were observed with respect to the specific volume and weight, internal structure, pH and titratable acidity among the bread samples obtained using different types and percentages of fibers. Storage time, at 30 up to 90 days, affected significantly the bread firmness and caused significant differences in 5-hydroxymethylfurfural (HMF) levels in all bread samples. In fortified breads with citrus fibers the yeast and mold counts showed values of approximately 1 log10 cfu/g for the first 30 days and 3.5 log10 cfu/g at the end of storage. The results of the sensory evaluation highlight that loaves enriched with blood orange and lemon fibers showed a citrus flavor but had a similar overall evaluation respect to control bread produced without addition of citrus fiber. The results of this study showed that the addition up to 2% of blood orange and lemon fibers in wheat whole durum flour is a possible strategy to produce “high fibre” bread

Orange juice processing using a continuous flow ultrasound-assisted supercritical CO2 system: Microbiota inactivation and product quality

[EN] The feasibility of using supercritical CO2 assisted by ultrasound (SC-CO2-HPU) in continuous mode (3.06 min residence time) for the non-thermal pasteurization of orange juice was evaluated. The proposed technology was effective for microbial inactivation; complete inactivation was obtained for E. coli and total aerobic mesophilic bacteria while 99.7% reduction for S. cerevisiae. Results showed that the SC-CO2-HPU treatment brought about small changes in the pH, Brix and titratable acidity of the juice. Furthermore, although SC-CO2-HPU technology produced a higher browning index (211%) and greater changes in color, it was possible to improve the cloud of juice by 173%; what is more, a smaller percentage of phenolic compounds (6.5%) and ascorbic acid (5.5%) was lost compared to the thermally pasteurized juice (10% decrease in both parameters). Moreover, the antioxidant capacity could be increased (12%) with respect to the natural juice. Therefore, SC-CO2-HPU technology appears to be effective for microbial pasteurization and the mild process conditions used could lead to an increase in the juice quality. Industrial relevance: The demand for high quality processed foods which preserve their natural and fresh-like characteristics has awakened a growing interest in non-thermal technologies. The ultrasound-assisted SC-CO2 continuous system is an innovative non-thermal technology that could represent a development in the area of emerging technologies. This technology allows high quality products to be obtained by preserving their natural bioactive compound content while maintaining their fresh-like organoleptic characteristics. In fact, food experts working in academia, industry or governmental agencies worldwide foresee that non-thermal emerging technologies will be among the most impactful novel food processing technologies for the next decade in terms of product commercialization.This work was supported by the PROMETEOII\2014\005 project financed by the Generalitat Valenciana (Conselleria d'Educació, Cultura i Esport, Valencia, Spain). The authors acknowledge the Consejo Nacional de Ciencia y Tecnología (CONACyT) for the scholarship awarded to PhD Student Paniagua-Martínez, I.Paniagua-Martínez, I.; Mulet Pons, A.; García Alvarado, MÁ.; Benedito Fort, JJ. (2018). Orange juice processing using a continuous flow ultrasound-assisted supercritical CO2 system: Microbiota inactivation and product quality. Innovative Food Science & Emerging Technologies. 47:362-370. https://doi.org/10.1016/j.ifset.2018.03.024S3623704

Beneficial Effects of Pomegranate Peel Extract and Probiotics on Pre-adipocyte Differentiation

The beneficial effects of pomegranate are due to the ellagitannins and anthocyanins content, which are protective toward a wide variety of diseases including inflammatory diseases. Many investigators have reported that pomegranate waste (peel and seeds) extracts, made from waste product of industrial processing, show free radical scavenger and a potent antioxidant capacity. Pomegranate extracts (PEs) were also reported to possess noteworty antibacterial, antiviral, hypolipidemic, and anti-inflammatory bioactivities thanks to the polyphenolic compounds content, which includes punicalagins, gallic acid, and ellagic acid derivatives. The focus of the present manuscript was to study the prebiotic potentiality of a PE, soluble in water, and characterized through HPLC–PDA–ESI/MSn for its phenolic content. Moreover, since it has been reported that pomegranate extracts decreased the level of lipids in the blood and that a number of probiotic strains have been shown to affect adipogenesis in cell culture, this study was also performed to test the in vitro effects of PE and probiotic L. rhamnosus GG ATCC 53103 strain (LGG) on 3T3-L1 cell line. PE and probiotics substantially reduced the triglyceride content and intracellular lipid increase, compared to the control group. However, the combination treatment of PE and LGG filtered spent broth (SB) was the most effective in reducing triglyceride content and intracellular lipid accumulation. The mRNA expression levels of the main transcriptional factors implicated in adipocyte differentiation were substantially lower in 3T3-L1 cells treated with PE and LGG filtered SB. These results evidenced that a synergistic effect of probiotics and polyphenols contained in PE may affect in vitro adipogenesis and may contribute in development of new nutraceutical/probiotic-based remedies to prevent and to treat obesity

Use of antibiotic spacers for knee endoprosthesis infections treatment

OBJCTIVE: The aim of this study is to evaluate the use of cement spacers impregnated with antibiotics for the treatment of infections in the nonconventional endoprostheses of the knee. METHODOLOGY: We have treated seven patients since 2004 (of which six were submitted to surgery in our service and one patient had been submitted to a primary tumor surgery in another removal service) with deep infection in knee tumor prosthesis. All patients were submitted to endoprosthesis removal and reconstructed with antibiotic cement spacer. All patients were monitored both clinically and by lab tests as for monitoring the evolution, being considered able for reviews after 6 (six) months without infections signs. RESULTS: We have noted a small predominance of infectious processes on the prosthesis inserted on proximal tibia as compared with distal femur (57.1% x 42.9%). The mean follow-up time of patients was 68.2 months. During the follow up, one patient died as a result of the root disease. Six patients out of seven were regarded as cured and one persisted with infection signs and symptoms. CONCLUSION: The results obtained up to date have motivated us to continue using this method of treatment.OBJETIVO: O objetivo do estudo é avaliar a utilização dos espaçadores de cimento acrílico com antibiótico no tratamento das infecções em endopróteses não convencionais de joelho. MÉTODO: Desde de 2004 foram tratados sete pacientes (seis pacientes operados no nosso serviço e um paciente que havia sido submetido a cirurgia primária do tumor em outro serviço) com infecção peri-endoprótese não convencional de joelho. Todos pacientes foram submetidos a retirada da endoprótese e reconstrução com espaçador com cimento acrílico com antibiótico. Todos os pacientes foram monitorados clínica e laboratorialmente quanto ao controle da evolução, sendo considerados aptos para a revisão e recolocação de endoprótese após 06 (seis) meses sem sinais infecciosos RESULTADOS: Notamos um discreto predomínio do do processo infeccioso nas próteses realizadas na tíbia proximal em comparação com o fêmur distal (57,1% x 42,9%). O seguimento médio dos pacientes foi 68,2 meses. Durante o seguimento, um paciente faleceu devido a doença de base. Dos sete pacientes, 6 foram considerados curados e um persistiu com sinais e sintomas de infecção. CONCLUSÃO: Os resultados obtidos até o momento tem motivado a continuidade deste método de tratamento.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de Ortopedia e TraumatologiaUNIFESP, EPM, Depto. de Ortopedia e TraumatologiaSciEL