Loss of S-nitrosoglutathione reductase disturbs phytohormone homeostasis and regulates shoot side branching and fruit growth in tomato

S-Nitrosoglutathione plays a central role in nitric oxide (NO) homeostasis, and S-nitrosoglutathione reductase (GSNOR) regulates the cellular levels of S-nitrosoglutathione across kingdoms. Here, we investigated the role of endogenous NO in shaping shoot architecture and controlling fruit set and growth in tomato (Solanum lycopersicum). SlGSNOR silencing promoted shoot side branching and led to reduced fruit size, negatively impacting fruit yield. Greatly intensified in slgsnor knockout plants, these phenotypical changes were virtually unaffected by SlGSNOR overexpression. Silencing or knocking out of SlGSNOR intensified protein tyrosine nitration and S-nitrosation and led to aberrant auxin production and signaling in leaf primordia and fruit-setting ovaries, besides restricting the shoot basipetal polar auxin transport stream. SlGSNOR deficiency triggered extensive transcriptional reprogramming at early fruit development, reducing pericarp cell proliferation due to restrictions on auxin, gibberellin, and cytokinin production and signaling. Abnormal chloroplast development and carbon metabolism were also detected in early-developing NO-overaccumulating fruits, possibly limiting energy supply and building blocks for fruit growth. These findings provide new insights into the mechanisms by which endogenous NO fine-tunes the delicate hormonal network controlling shoot architecture, fruit set, and post-anthesis fruit development, emphasizing the relevance of NO-auxin interaction for plant development and productivity.This work was supported by the São Paulo Research Foundation (FAPESP) (grants 16/02033-1, 16/01128-9 17/17935-3, 18/16389-8, 18/06436-9, 20/03720-8, 21/05714-8), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (grants 422287/2018-0, 305012/2018-5), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES) – Finance Code 00

Progress in the determination of the J/ψ−πJ/\psi-\pi cross section

Improving previous calculations, we compute the $J/\psi \pi\to {charmed mesons}$ cross section using QCD sum rules. Our sum rules for the $J/\psi \pi\to \bar{D} D^*$, $D \bar{D}^*$, ${\bar D}^* D^*$ and ${\bar D} D$ hadronic matrix elements are constructed by using vaccum-pion correlation functions, and we work up to twist-4 in the soft-pion limit. Our results suggest that, using meson exchange models is perfectly acceptable, provided that they include form factors and that they respect chiral symmetry. After doing a thermal average we get $\sim 0.3$ mb at T=150\MeV.Comment: 22 pages, RevTeX4 including 7 figures in ps file

Permanent Genetic Resources added to Molecular Ecology Resources Database 1 February 2013-31 March 2013

This article documents the addition of 142 microsatellite marker loci to the Molecular Ecology Resources database. Loci were developed for the following species: Agriophyllum squarrosum, Amazilia cyanocephala, Batillaria attramentaria, Fungal strain CTeY1 (Ascomycota), Gadopsis marmoratus, Juniperus phoenicea subsp. turbinata, Liriomyza sativae, Lupinus polyphyllus, Metschnikowia reukaufii, Puccinia striiformis and Xylocopa grisescens. These loci were cross-tested on the following species: Amazilia beryllina, Amazilia candida, Amazilia rutila, Amazilia tzacatl, Amazilia violiceps, Amazilia yucatanensis, Campylopterus curvipennis, Cynanthus sordidus, Hylocharis leucotis, Juniperus brevifolia, Juniperus cedrus, Juniperus osteosperma, Juniperus oxycedrus, Juniperus thurifera, Liriomyza bryoniae, Liriomyza chinensis, Liriomyza huidobrensis and Liriomyza trifolii. © 2013 John Wiley & Sons Ltd.Peer Reviewe

Taxonomy based on science is necessary for global conservation

Peer reviewe

Patch-level facilitation fosters high-Andean plant diversity at regional scales

Aim: Local effects of ecosystem engineers on biodiversity can scale up to the landscape level, stressing the importance of ecological processes as determinants of species richness at larger spatial scales. In harsh environments, cushion plants often act as ecosystem engineers given their ability to buffer extreme abiotic conditions, thus providing unique and more favorable niches for the establishment of less stress-tolerant plant species. We assessed if facilitation by cushion plants influences patterns of plant diversity at increasing spatial scales. Location: Northern Patagonian Andes, Argentina. Methods: Based on plant species records within and outside cushions, we compared plant diversity in the presence and absence of cushions at the community, mountain and regional scale. Specifically, observed and estimated numbers of species occurring in either cushion plants or in the surrounding open areas were compared with the species numbers of the open areas. Results: The presence of cushion plants significantly increased species richness at all spatial levels analyzed. At the patch level, a higher number of species was recorded growing within cushion plants than in open area plots of similar size. Consistently, hypothetical communities lacking cushion plants showed significantly lower species richness than observed communities with cushion plants. These differences in species richness at patch and community scales increased with altitude, hence facilitation by cushion plants became more important at higher elevations. Moreover, according to asymptotic non-parametric estimators, cushion plants could increase overall regional species richness up to 40%. Conclusions: Our findings suggest that about one third of the high-Andean flora of the region owes its presence to the existence of nursing plants, defying the view that abiotic factors alone are enough to fully determine species occurrences at large spatial scales. This study provides evidence that local-scale ecological processes can be crucial in promoting and maintaining biodiversity at any spatial scale.Fil: Gavini, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Ezcurra, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; ArgentinaFil: Aizen, Marcelo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigaciones en Biodiversidad y Medioambiente. Universidad Nacional del Comahue. Centro Regional Universidad Bariloche. Instituto de Investigaciones en Biodiversidad y Medioambiente; Argentin