Workplace trade union engagement with European Works Councils and transnational agreements: the case of Volkswagen Europe

This article examines two transnational agreements signed by the Volkswagen European and Global Works Councils, considering their interlinked implementation within subsidiaries in Britain, Italy, Spain and Germany. We demonstrate differing stances and some uncertainty towards principles of co-management, social dialogue and codetermination. These agreements have improved local industrial relations and strengthened cross-national interaction between employee representatives, despite significant differences in orientation regarding how unions should engage with management. However, the emerging international framework has not led to a clear politics of incorporation, with local trade unions being well aware of the risks of co-management and a more business-oriented relationship

Biochemical Characterization of a Trypanosomatid Isolated from the Plant Amaranthus retroflexus

A protozoan flagelate has recently been isolated from Amaranthus retroflexus. This plant grows near economically important crops in southeastern Spain, which are known to be parasitized by Phytomonas spp. The present study focuses on the characterization of the energy metabolism of this new isolate. These flagellates utilize glucose efficiently as their primary energy source, although they are unable to completely degrade it. They excrete ethanol, acetate, glycine, and succinate in lower amount, as well as ammonium. The presence of glycosomes was indicated by the early enzymes of the glycolytic pathway, one enzyme of the glycerol pathway (glycerol kinase), and malate dehydrogenase. No evidence of a fully functional citric-acid cycle was found. In the absence of catalase activity, these flagellates showed significant superoxide dismutase activity located in the glycosomal and cytosolic fractions. These trypanosomes, despite being morphologically and metabolically similar to other Phytomonas isolated from the same area, showed significant differences, suggesting that they are phylogenetically different species

Contribution of microscopy for understanding the mechanism of action against trypanosomatids

Transmission electron microscopy (TEM) has proved to be a useful tool to study the ultrastructural alterations and the target organelles of new antitrypanosomatid drugs. Thus, it has been observed that sesquiterpene lactones induce diverse ultrastructural alterations in both T. cruzi and Leishmania spp., such as cytoplasmic vacuolization, appearance of multilamellar structures, condensation of nuclear DNA, and, in some cases, an important accumulation of lipid vacuoles. This accumulation could be related to apoptotic events. Some of the sesquiterpene lactones (e.g., psilostachyin) have also been demonstrated to cause an intense mitochondrial swelling accompanied by a visible kinetoplast deformation as well as the appearance of multivesicular bodies. This mitochondrial swelling could be related to the generation of oxidative stress and associated to alterations in the ergosterol metabolism. The appearance of multilamellar structures and multiple kinetoplasts and flagella induced by the sesquiterpene lactone psilostachyin C indicates that this compound would act at the parasite cell cycle level, in an intermediate stage between kinetoplast segregation and nuclear division. In turn, the diterpene lactone icetexane has proved to induce the external membrane budding on T. cruzi together with an apparent disorganization of the pericellar cytoskeleton. Thus, ultrastructural TEM studies allow elucidating the possible mechanisms and the subsequent identification of molecular targets for the action of natural compounds on trypanosomatids.Fil: Lozano, Esteban Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Spina Zapata, Renata María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Barrera, Patricia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Tonn, Carlos Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Sosa Escudero, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentin

Stellar metallicity from optical and UV spectral indices: test case for WEAVE-StePS

Context. The upcoming generation of optical spectrographs on four meter-class telescopes, with their huge multiplexing capabilities, excellent spectral resolution, and unprecedented wavelength coverage, will provide high-quality spectra for thousands of galaxies. These data will allow us to examine of the stellar population properties at intermediate redshift, an epoch that remains unexplored by large and deep surveys. Aims. We assess our capability to retrieve the mean stellar metallicity in galaxies at different redshifts and signal-to-noise ratios (S/N), while simultaneously exploiting the ultraviolet (UV) and optical rest-frame wavelength coverage. Methods. The work is based on a comprehensive library of spectral templates of stellar populations, covering a wide range of age and metallicity values and built assuming various star formation histories (SFHs), to cover an observable parameter space with diverse chemical enrichment histories and dust attenuation. We took into account possible observational errors, simulating realistic observations of a large sample of galaxies carried out with WEAVE at the William Herschel Telescope at different redshifts and S/N values. We measured all the available and reliable indices on the simulated spectra and on the comparison library. We then adopted a Bayesian approach to compare the two sets of measurements in order to obtain the probability distribution of stellar metallicity with an accurate estimate of the uncertainties. Results. The analysis of the spectral indices has shown how some mid-UV indices, such as BL3580 and Fe3619, can provide reliable constraints on stellar metallicity, along with optical indicators. The analysis of the mock observations has shown that even at S/N = 10, the metallicity can be derived within 0.3 dex, in particular, for stellar populations older than 2 Gyr. The S/N value plays a crucial role in the uncertainty of the estimated metallicity and so, the differences between S/N = 10 and S/N = 30 are quite large, with uncertainties of ∼ 0.15 dex in the latter case. On the contrary, moving from S/N = 30 to S/N = 50, the improvement on the uncertainty of the metallicity measurements is almost negligible. Our results are in good agreement with other theoretical and observational works in the literature and show how the UV indicators, coupled with classic optical ones, can be advantageous in constraining metallicities. Conclusions. We demonstrate that a good accuracy can be reached on the spectroscopic measurements of the stellar metallicity of galaxies at intermediate redshift, even at low S/N, when a large number of indices can be employed, including some UV indices. This is very promising for the upcoming surveys carried out with new, highly multiplexed, large-field spectrographs, such as StePS at the WEAVE and 4MOST, which will provide spectra of thousands of galaxies covering large spectral ranges (between 3600 and 9000 Å in the observed frame) at relatively high S/N (> 10 Å−1 )

Calidad de vida en asistentes a un programa de actividad física en Bogotá, Colombia

The rise of chronic noncommunicable diseases in older adults is a global public health challenge, so actions to prevent or mitigate them are necessary to improve population health but quality of life. The objective focused on and rated the quality of life in users who regularly attend the Recreovía program. Non-experimental study with descriptive cross-sectional design, conducted in 280 adult women with an average age of 56.71 ± to 10.79 years, inhabitants of the city of Bogotá, who attend the Recreovía program 3 times a week. Quality of life was evaluated with WHOQOL-BREF. A statistically significant relationship was found between quality of life and socioeconomic stratum (p=0,002), between quality of life and physical health (p<0,001) and quality of life and hypertension (p=0,003). The perception of quality of life was weighted as good as referred to by the participants; as there is a relationship between intermediate and structural determinants, it encourages decision-making that actions in the population must transcend beyond collective programs but affect the living and health conditions of the population.El aumento de Enfermedades crónicas no transmisibles en los adultos mayores es un reto en salud pública a nivel mundial, por lo que las acciones que se hagan para prevenir o mitigar estas son necesarias para mejorar la salud de la población sino la calidad de vida. El objetivo se centró en evaluar la calidad de vida en las usuarias que asisten de manera regular al programa de Recreovía. Estudio no experimental con diseño descriptivo de corte transversal, realizado en 280 mujeres adultas con una edad promedio de 56.71 ± 10.79 años, habitantes de la ciudad de Bogotá, que asisten 3 veces por semana al programa de Recreovía. Se evalúo la calidad de vida con el WHOQOL-BREF. Se encontró relación estadísticamente significativa entre la calidad de vida y el estrato socioeconómico (p=0.002), entre la calidad de vida y la salud física (p<0.001) y la calidad de vida e Hipertensión (p=0.003). La percepción de calidad de vida fue ponderada como buena de acuerdo con lo referido por las participantes; al existir relación entre determinantes intermedios y estructurales, alienta a tomadores de decisión que las acciones en la población deben trascender más allá de programas colectivos, sino que afecten las condiciones de vida y salud de la población

HERC6 is the main E3 ligase for global ISG15 conjugation in mouse cells

Type I interferon (IFN) stimulates expression and conjugation of the ubiquitin-like modifier IFN-stimulated gene 15 (ISG15), thereby restricting replication of a wide variety of viruses. Conjugation of ISG15 is critical for its antiviral activity in mice. HECT domain and RCC1-like domain containing protein 5 (HerC5) mediates global ISGylation in human cells, whereas its closest relative, HerC6, does not. So far, the requirement of HerC5 for ISG15-mediated antiviral activity has remained unclear. One of the main obstacles to address this issue has been that no HerC5 homologue exists in mice, hampering the generation of a good knock-out model. However, mice do express a homologue of HerC6 that, in contrast to human HerC6, can mediate ISGylation. Here we report that the mouse HerC6 N-terminal RCC1-like domain (RLD) allows ISG15 conjugation when replacing the corresponding domain in the human HerC6 homologue. In addition, sequences in the C-terminal HECT domain of mouse HerC6 also appear to facilitate efficient ISGylation. Mouse HerC6 paralleled human HerC5 in localization and IFN-inducibility. Moreover, HerC6 knock-down in mouse cells abolished global ISGylation, whereas its over expression enhanced the IFNβ promoter and conferred antiviral activity against vesicular stomatitis virus and Newcastle disease virus. Together these data indicate that HerC6 is likely the functional counterpart of human HerC5 in mouse cells, suggesting that HerC6-/-mice may provide a feasible model to study the role of human HerC5 in antiviral responses

Autoimmunity plays a role in the onset of diabetes after 40 years of age

AIMS/HYPOTHESIS: Type 1 and type 2 diabetes differ with respect to pathophysiological factors such as beta cell function, insulin resistance and phenotypic appearance, but there may be overlap between the two forms of diabetes. However, there are relatively few prospective studies that have characterised the relationship between autoimmunity and incident diabetes. We investigated associations of antibodies against the 65 kDa isoform of GAD (GAD65) with type 1 diabetes and type 2 diabetes genetic risk scores and incident diabetes in adults in European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct, a case-cohort study nested in the EPIC cohort. METHODS: GAD65 antibodies were analysed in EPIC participants (over 40 years of age and free of known diabetes at baseline) by radioligand binding assay in a random subcohort (n = 15,802) and in incident diabetes cases (n = 11,981). Type 1 diabetes and type 2 diabetes genetic risk scores were calculated. Associations between GAD65 antibodies and incident diabetes were estimated using Prentice-weighted Cox regression. RESULTS: GAD65 antibody positivity at baseline was associated with development of diabetes during a median follow-up time of 10.9 years (HR for GAD65 antibody positive vs negative 1.78; 95% CI 1.43, 2.20) after adjustment for sex, centre, physical activity, smoking status and education. The genetic risk score for type 1 diabetes but not type 2 diabetes was associated with GAD65 antibody positivity in both the subcohort (OR per SD genetic risk 1.24; 95% CI 1.03, 1.50) and incident cases (OR 1.97; 95% CI 1.72, 2.26) after adjusting for age and sex. The risk of incident diabetes in those in the top tertile of the type 1 diabetes genetic risk score who were also GAD65 antibody positive was 3.23 (95% CI 2.10, 4.97) compared with all other individuals, suggesting that 1.8% of incident diabetes in adults was attributable to this combination of risk factors. CONCLUSIONS/INTERPRETATION: Our study indicates that incident diabetes in adults has an element of autoimmune aetiology. Thus, there might be a reason to re-evaluate the present subclassification of diabetes in adulthood

Autoimmunity plays a role in the onset of diabetes after 40 years of age

AIMS/HYPOTHESIS: Type 1 and type 2 diabetes differ with respect to pathophysiological factors such as beta cell function, insulin resistance and phenotypic appearance, but there may be overlap between the two forms of diabetes. However, there are relatively few prospective studies that have characterised the relationship between autoimmunity and incident diabetes. We investigated associations of antibodies against the 65 kDa isoform of GAD (GAD65) with type 1 diabetes and type 2 diabetes genetic risk scores and incident diabetes in adults in European Prospective Investigation into Cancer and Nutrition (EPIC)-InterAct, a case-cohort study nested in the EPIC cohort. METHODS: GAD65 antibodies were analysed in EPIC participants (over 40 years of age and free of known diabetes at baseline) by radioligand binding assay in a random subcohort (n = 15,802) and in incident diabetes cases (n = 11,981). Type 1 diabetes and type 2 diabetes genetic risk scores were calculated. Associations between GAD65 antibodies and incident diabetes were estimated using Prentice-weighted Cox regression. RESULTS: GAD65 antibody positivity at baseline was associated with development of diabetes during a median follow-up time of 10.9 years (HR for GAD65 antibody positive vs negative 1.78; 95% CI 1.43, 2.20) after adjustment for sex, centre, physical activity, smoking status and education. The genetic risk score for type 1 diabetes but not type 2 diabetes was associated with GAD65 antibody positivity in both the subcohort (OR per SD genetic risk 1.24; 95% CI 1.03, 1.50) and incident cases (OR 1.97; 95% CI 1.72, 2.26) after adjusting for age and sex. The risk of incident diabetes in those in the top tertile of the type 1 diabetes genetic risk score who were also GAD65 antibody positive was 3.23 (95% CI 2.10, 4.97) compared with all other individuals, suggesting that 1.8% of incident diabetes in adults was attributable to this combination of risk factors. CONCLUSIONS/INTERPRETATION: Our study indicates that incident diabetes in adults has an element of autoimmune aetiology. Thus, there might be a reason to re-evaluate the present subclassification of diabetes in adulthood

The regeneration capacity of the flatworm Macrostomum lignano—on repeated regeneration, rejuvenation, and the minimal size needed for regeneration

The lion’s share of studies on regeneration in Plathelminthes (flatworms) has been so far carried out on a derived taxon of rhabditophorans, the freshwater planarians (Tricladida), and has shown this group’s outstanding regeneration capabilities in detail. Sharing a likely totipotent stem cell system, many other flatworm taxa are capable of regeneration as well. In this paper, we present the regeneration capacity of Macrostomum lignano, a representative of the Macrostomorpha, the basal-most taxon of rhabditophoran flatworms and one of the most basal extant bilaterian protostomes. Amputated or incised transversally, obliquely, and longitudinally at various cutting levels, M. lignano is able to regenerate the anterior-most body part (the rostrum) and any part posterior of the pharynx, but cannot regenerate a head. Repeated regeneration was observed for 29 successive amputations over a period of almost 12 months. Besides adults, also first-day hatchlings and older juveniles were shown to regenerate after transversal cutting. The minimum number of cells required for regeneration in adults (with a total of 25,000 cells) is 4,000, including 160 neoblasts. In hatchlings only 1,500 cells, including 50 neoblasts, are needed for regeneration. The life span of untreated M. lignano was determined to be about 10 months

Optomechanical Crystals

Structured, periodic optical materials can be used to form photonic crystals capable of dispersing, routing, and trapping light. A similar phenomena in periodic elastic structures can be used to manipulate mechanical vibrations. Here we present the design and experimental realization of strongly coupled optical and mechanical modes in a planar, periodic nanostructure on a silicon chip. 200-Terahertz photons are co-localized with mechanical modes of Gigahertz frequency and 100-femtogram mass. The effective coupling length, which describes the strength of the photon-phonon interaction, is as small as 2.9 microns, which, together with minute oscillator mass, allows all-optical actuation and transduction of nanomechanical motion with near quantum-limited sensitivity. Optomechanical crystals have many potential applications, from RF-over-optical communication to the study of quantum effects in mesoscopic mechanical systems.Comment: 16 pages, 7 figure