Production and quality of floral buds for rose cv. Vega (Rosa SP.) cultivated under plastic with different years of use

Objetivou-se, neste trabalho, avaliar a produção e qualidade de botões de rosa cultivar Vega ( Rosa sp. ), cultivada sob cobertura plástica com diferentes anos de uso. Para tanto foram selecionados 15 vãos de estufas com plásticos colocados em 1998, 1999, 2002, 2003, e testemunha (sem uso anterior), com três repetições, avaliados por duas maneiras: na primeira os frutos foram colhidos através de uma poda (como procedimento do cultivo comercial); na segunda foi realizada uma poda rasa em uma área de 1 m² para poder acompanhar o crescimento da haste floral desde o início da formação até a fase de botão floral. No primeiro caso foram realizadas 4 coletas ao longo do período do experimento, nas quais foram coletadas 4 hastes florais por tratamento, totalizando 60 flores por data de coleta. No segundo caso foi avaliado o aparecimento dos botões florais dos novos brotos em quatro datas até a formação das hastes florais comerciais, e então coletadas 13 hastes florais por tratamento. Não houve diferença estatística entre os anos de uso dos plásticos de 1998, 1999, 2002 e 2003 nas variáveis: comprimento, diâmetro e comprimento dos botões florais, massa fresca e seca das pétalas, para as condições e cultivar analisados. Com isso o produtor ganha um período maior de utilização do plástico gerando um custo menor, sem perdas na produção.The aim of this study was to evaluate the production and quality of floral buds of rose cv. Vega (Rosa sp.) cultivated underneath a plastic film used as greenhouse covering, with different years of use. In order to evaluate the bud production and quality, 15 greenhouse gaps with plastic covering dating from 1998, 1999, 2002, 2003, were selected; and the control covering (never used before) with three replications each. Two types of evaluation were made for this analysis: the first one, the fruits were harvested by means of pruning (as the commercial use standard procedure); in the second evaluation a clear cut pruning was conducted in an area of 1m² with the purpose of determining the development of the flower buds from the pruning to the commercial flower stalk. On the first evaluation, 4 harvestings were conducted during the entire experiment period, in which 4 flower stalks per treatments were collected, totaling 60 flowers per collecting date. On the second evaluation, floral buds emerging on new shoots were determined in four different dates until commercial flower stalks complete formation was achieved, then 13 flower stalks per treatment were collected. There was no statistical difference among the plastic films with different years of use regarding stalk length and diameter, flower bud diameter and length, dry and fresh petal mass under the analyzed conditions and cultivars, translating into a larger period of plastic covering use without loss in the production at a low production cost.Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES

Production and quality of floral buds for rose cv. Vega (Rosa SP.) cultivated under plastic with different years of use

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Objetivou-se, neste trabalho, avaliar a produção e qualidade de botões de rosa cultivar Vega ( Rosa sp. ), cultivada sob cobertura plástica com diferentes anos de uso. Para tanto foram selecionados 15 vãos de estufas com plásticos colocados em 1998, 1999, 2002, 2003, e testemunha (sem uso anterior), com três repetições, avaliados por duas maneiras: na primeira os frutos foram colhidos através de uma poda (como procedimento do cultivo comercial); na segunda foi realizada uma poda rasa em uma área de 1 m² para poder acompanhar o crescimento da haste floral desde o início da formação até a fase de botão floral. No primeiro caso foram realizadas 4 coletas ao longo do período do experimento, nas quais foram coletadas 4 hastes florais por tratamento, totalizando 60 flores por data de coleta. No segundo caso foi avaliado o aparecimento dos botões florais dos novos brotos em quatro datas até a formação das hastes florais comerciais, e então coletadas 13 hastes florais por tratamento. Não houve diferença estatística entre os anos de uso dos plásticos de 1998, 1999, 2002 e 2003 nas variáveis: comprimento, diâmetro e comprimento dos botões florais, massa fresca e seca das pétalas, para as condições e cultivar analisados. Com isso o produtor ganha um período maior de utilização do plástico gerando um custo menor, sem perdas na produção.The aim of this study was to evaluate the production and quality of floral buds of rose cv. Vega (Rosa sp.) cultivated underneath a plastic film used as greenhouse covering, with different years of use. In order to evaluate the bud production and quality, 15 greenhouse gaps with plastic covering dating from 1998, 1999, 2002, 2003, were selected; and the control covering (never used before) with three replications each. Two types of evaluation were made for this analysis: the first one, the fruits were harvested by means of pruning (as the commercial use standard procedure); in the second evaluation a clear cut pruning was conducted in an area of 1m² with the purpose of determining the development of the flower buds from the pruning to the commercial flower stalk. On the first evaluation, 4 harvestings were conducted during the entire experiment period, in which 4 flower stalks per treatments were collected, totaling 60 flowers per collecting date. On the second evaluation, floral buds emerging on new shoots were determined in four different dates until commercial flower stalks complete formation was achieved, then 13 flower stalks per treatment were collected. There was no statistical difference among the plastic films with different years of use regarding stalk length and diameter, flower bud diameter and length, dry and fresh petal mass under the analyzed conditions and cultivars, translating into a larger period of plastic covering use without loss in the production at a low production cost.33spe20112018Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES_Brasi

Production and quality of floral buds for rose cv. Vega (Rosa SP.) cultivated under plastic with different years of use

Objetivou-se, neste trabalho, avaliar a produção e qualidade de botões de rosa cultivar Vega ( Rosa sp. ), cultivada sob cobertura plástica com diferentes anos de uso. Para tanto foram selecionados 15 vãos de estufas com plásticos colocados em 1998, 1999, 2002, 2003, e testemunha (sem uso anterior), com três repetições, avaliados por duas maneiras: na primeira os frutos foram colhidos através de uma poda (como procedimento do cultivo comercial); na segunda foi realizada uma poda rasa em uma área de 1 m2 para poder acompanhar o crescimento da haste floral desde o início da formação até a fase de botão floral. No primeiro caso foram realizadas 4 coletas ao longo do período do experimento, nas quais foram coletadas 4 hastes florais por tratamento, totalizando 60 flores por data de coleta. No segundo caso foi avaliado o aparecimento dos botões florais dos novos brotos em quatro datas até a formação das hastes florais comerciais, e então coletadas 13 hastes florais por tratamento. Não houve diferença estatística entre os anos de uso dos plásticos de 1998, 1999, 2002 e 2003 nas variáveis: comprimento, diâmetro e comprimento dos botões florais, massa fresca e seca das pétalas, para as condições e cultivar analisados. Com isso o produtor ganha um período maior de utilização do plástico gerando um custo menor, sem perdas na produção.33esp.20112018COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informaçãoThe aim of this study was to evaluate the production and quality of floral buds of rose cv. Vega (Rosa sp.) cultivated underneath a plastic film used as greenhouse covering, with different years of use. In order to evaluate the bud production and quality, 15 greenhouse gaps with plastic covering dating from 1998, 1999, 2002, 2003, were selected; and the control covering (never used before) with three replications each. Two types of evaluation were made for this analysis: the first one, the fruits were harvested by means of pruning (as the commercial use standard procedure); in the second evaluation a clear cut pruning was conducted in an area of 1m2 with the purpose of determining the development of the flower buds from the pruning to the commercial flower stalk. On the first evaluation, 4 harvestings were conducted during the entire experiment period, in which 4 flower stalks per treatments were collected, totaling 60 flowers per collecting date. On the second evaluation, floral buds emerging on new shoots were determined in four different dates until commercial flower stalks complete formation was achieved, then 13 flower stalks per treatment were collected. There was no statistical difference among the plastic films with different years of use regarding stalk length and diameter, flower bud diameter and length, dry and fresh petal mass under the analyzed conditions and cultivars, translating into a larger period of plastic covering use without loss in the production at a low production cost

Genome-wide association and HLA fine-mapping studies identify risk loci and genetic pathways underlying allergic rhinitis

Allergic rhinitis is the most common clinical presentation of allergy, affecting 400 million people worldwide, with increasing incidence in westernized countries1,2. To elucidate the genetic architecture and understand the underlying disease mechanisms, we carried out a meta-analysis of allergic rhinitis in 59,762 cases and 152,358 controls of European ancestry and identified a total of 41 risk loci for allergic rhinitis, including 20 loci not previously associated with allergic rhinitis, which were confirmed in a replication phase of 60,720 cases and 618,527 controls. Functional annotation implicated genes involved in various immune pathways, and fine mapping of the HLA region suggested amino acid variants important for antigen binding. We further performed genome-wide association study (GWAS) analyses of allergic sensitization against inhalant allergens and nonallergic rhinitis, which suggested shared genetic mechanisms across rhinitis-related traits. Future studies of the identified loci and genes might identify novel targets for treatment and prevention of allergic rhinitis

Milk quality improvement after implantation of good manufacturing practices in milking in 19 cities of the central region of Paraná / <br> Melhoria da qualidade do leite a partir da implantação de boas práticas de higiene na ordenha em 19 municípios da região central do Paraná

The milk production in Brazil presents efficiency and quality problems concerning the raw material, reducing its competitiveness. The poor quality of raw milk is associated with faults in handling and milking hygiene conditions, high indices of mastitis, inadequate maintenance and disinfection of the equipment, inefficient or inexistent refrigeration, disqualified workforce, among others. The aim of this study was to evaluate the efficiency of implantation of simple, inexpensive and easily adopted Good Manufacturing Practices (GMP) on dairy farms. Quality parameters researched were total bacterial count (TBC) and somatic cell count (SCC), which indicates respectively milking hygiene and mammary gland health. Forty six raw milk samples from 19 cities of the central region of Paraná were analyzed, among them, 32 (69,57%) had manual milking and 14 (30,43%) mechanic milking. The implantation of GMP resulted in average reduction of 87,90% to TBC on manual milking farms and 86,99% on mechanic milking farms. Regarding SCC, the average reduction was 33,94% in manual milking farms and 51,85% in mechanics milking farms. The implemented practices had shown efficient in all of the studied conditions of production and installations.<p><p>O setor leiteiro brasileiro apresenta problemas de eficiência produtiva e de qualidade da matéria-prima e, por isso, perde em competitividade. A má qualidade do leite cru está relacionada a fatores como deficiências no manejo e higiene da ordenha, índices elevados de mastite, manutenção e desinfecção inadequadas dos equipamentos, refrigeração ineficiente ou inexistente e mão de obra desqualificada, entre outros. O objetivo deste trabalho foi avaliar a eficiência da implantação de Boas Práticas na ordenha na ordenha, simples e baratas, que pudessem ser facilmente introduzidas pelos produtores de leite. Os parâmetros de qualidade considerados foram a contagem de células somáticas (CCS), indicativo de sanidade da glândula mamária, e a contagem bacteriana total (CBT), indicativo de higiene de ordenha. Foram analisadas 46 amostras de leite cru de 19 municípios da região central do Paraná, sendo 32 (69,57%) de propriedades com ordenha manual e 14 (30,43%) com ordenha mecânica. Após a implantação das práticas houve uma redução média de 87,90% na CBT nas propriedades com ordenha manual e 86,99% nas propriedades com ordenha mecânica. Com relação a CCS, a redução média foi de 33,94% em propriedades com ordenha manual e 51,85% em propriedades com ordenha mecânica. As práticas implantadas se mostraram eficientes nos diferentes sistemas de produção

Comparative retrospective study on the modalities of biopsying peripheral neuroblastic tumors: a report from the Italian Pediatric Surgical Oncology Group (GICOP)

Background: Peripheral neuroblastic tumors are the most common extracranial solid neoplasms in children. Early and adequate tissue sampling may speed up the diagnostic process and ensure a prompt start of optimal treatment whenever needed. Different biopsy techniques have been described. The purpose of this multi-center study is to evaluate the accuracy and safety of the various examined techniques and to determine whether a preferential procedure exists. Methods: All children who underwent a biopsy, from January 2010 to December 2014, as a result of being diagnosed with a peripheral neuroblastic tumor, were retrospectively reviewed. Data collected included patients\u2019 demographics, clinical presentation, intraoperative technical details, postoperative parameters, complications, and histology reports. The Mann\u2013Whitney U and Fisher's exact tests were used for statistical analysis. Results: The cohort included 100 patients, 32 of whom underwent an incisional biopsy (performed through open or minimally invasive access) (Group A), and the remaining 68 underwent multiple needle-core biopsies (either imaging-guided or laparoscopy/thoracoscopy-assisted) (Group B). Comparing the two groups revealed that Group A patients had a higher rate of complications, a greater need for postoperative analgesia, and required red blood cell transfusion more often. Overall adequacy rate was 94%, without significant differences between the two groups (100% vs. 91.2% for Group A and Group B, respectively, P = 0.0933). Conclusions: Both incision and needle-core biopsying methods provided sub-optimal to optimal sampling adequacy rates in children affected by peripheral neuroblastic tumors. However, the former method was associated with a higher risk of both intraoperative and postoperative complications compared with the latter

Resection of primary tumor in stage 4S neuroblastoma: a second study by the Italian Neuroblastoma Group

Purpose To clarify the role of primary tumor resection in stage 4S neuroblastoma. Methods We investigated a cohort of 172 infants diagnosed with stage 4S neuroblastoma between 1994 and 2013. Of 160 evaluable patients, 62 underwent upfront resection of the primary tumor and 98 did not. Results Five-year progression-free and overall survival were significantly better in those who had undergone upfront surgery (83.6% vs 64.2% and 96.8% vs 85.7%, respectively). One post-operative death and four non-fatal complications occurred in the resection group. Three patients who had not undergone resection died of chemotherapy-related toxicity. Thirteen patients underwent late surgery to remove a residual tumor, without complications: all but one alive. Outcomes were better in patients diagnosed from 2000 onwards. Conclusion Infants diagnosed with stage 4S neuroblastoma who underwent upfront tumor resection had a better outcome. However, this result cannot be definitely attributed to surgery, since these patients were selected on the basis of their favorable presenting features. Although the question of whether to operate or not at disease onset is still unsolved, this study confirms the importance of obtaining enough adequate tumor tissue to enable histological and biological studies to properly address treatment, to achieve the best possible outcome