Comparaison des qualités sensorielles de la viande et de la carcasse d’agneaux élevés au pâturage en production biologique ou conventionnelle à deux niveaux de disponibilités en herbe

Nous avons comparé les qualités sensorielles des carcasses et des viandes d’agneaux engraissés au pâturage en élevage biologique ou conventionnel (O vs. C) à deux niveaux de disponibilités en herbe (Haut H vs. Bas L). Le profil de croissance a été maintenu similaire entre les deux systèmes de production. L’expérimentation a été conduite pendant deux années avec 12 agneaux mâles castrés de race Limousine dans chaque groupe OH, OL, CH et CL chaque année. Les traitements O et C différaient par le niveau de fertilisation azotée minérale épandu sur les parcelles. Les parcelles expérimentales étaient des repousses après fauche et elles étaient conduites en pâturage tournant pour conduire à un âge moyen des agneaux à l’abattage de 5 et 6 mois dans les lots H et L respectivement. Les côtelettes O ont été moins appréciées que les côtelettes C. L’indice de rouge du muscle longissimus thoracis et lumborum après 2h d’exposition à l’air a été plus élevé chez les agneaux L que chez les agneaux H, indiquant les effets possibles d’une intensification de l’élevage biologique à travers une augmentation du chargement

In situ Laser Induced Breakdown Spectroscopy as a tool to discriminate volcanic rocks and magmatic series, Iceland

This study evaluates the potentialities of a lab-made pLIBS (portable Laser-Induced Breakdown Spectroscopy) to sort volcanic rocks belonging to various magmatic series. An in-situ chemical analysis of 19 atomic lines, including Al, Ba, Ca, Cr, Cu, Fe, Mg, Mn, Na, Si, Sr and Ti, from 21 sampled rocks was performed during a field exploration in Iceland. Iceland was chosen both for the various typologies of volcanic rocks and the rugged conditions in the field in order to test the sturdiness of the pLIPS. Elemental compositions were also measured using laboratory ICP-AES measurements on the same samples. Based on these latter results, which can be used to identify three different groups of volcanic rocks, a classification model was built in order to sort pLIBS data and to categorize unknown samples. Using a reliable statistical scheme applied to LIBS compositional data, the classification capability of the pLIBS system is clearly demonstrated (90-100% success rate). Although this prototype does not provide quantitative measurements, its use should be of particular interest for future geological field investigations

Connecting solar flare hard X-ray spectra to in situ electron spectra A comparison of RHESSI and STEREO/SEPT observations

Aims. We aim to constrain the acceleration, injection, and transport processes of flare-accelerated energetic electrons by comparing their characteristics at the Sun with those injected into interplanetary space.Methods. We have identified 17 energetic electron events well-observed with the SEPT instrument aboard STEREO which show a clear association with a hard X-ray (HXR) flare observed with the RHESSI spacecraft. We compare the spectral indices of the RHESSI HXR spectra with those of the interplanetary electrons. Because of the frequent double-power-law shape of the in situ electron spectra, we paid special attention to the choice of the spectral index used for comparison.Results. The time difference between the electron onsets and the associated type III and microwave bursts suggests that the electron events are detected at 1 AU with apparent delays ranging from 9 to 41 min. While the parent solar activity is clearly impulsive, also showing a high correlation with extreme ultraviolet jets, most of the studied events occur in temporal coincidence with coronal mass ejections (CMEs). In spite of the observed onset delays and presence of CMEs in the low corona, we find a significant correlation of about 0.8 between the spectral indices of the HXR flare and the in situ electrons. The correlations increase if only events with significant anisotropy are considered. This suggests that transport effects can alter the injected spectra leading to a strongly reduced imprint of the flare acceleration.Conclusions. We conclude that interplanetary transport effects must be taken into account when inferring the initial acceleration of solar energetic electron events. Although our results suggest a clear imprint of flare acceleration for the analyzed event sample, a secondary acceleration might be present which could account for the observed delays. However, the limited and variable pitch-angle coverage of SEPT could also be the reason for the observed delays

COIL: a methodology for evaluating malarial complexity of infection using likelihood from single nucleotide polymorphism data.

International audienceComplex malaria infections are defined as those containing more than one genetically distinct lineage of Plasmodium parasite. Complexity of infection (COI) is a useful parameter to estimate from patient blood samples because it is associated with clinical outcome, epidemiology and disease transmission rate. This manuscript describes a method for estimating COI using likelihood, called COIL, from a panel of bi-allelic genotyping assays. COIL assumes that distinct parasite lineages in complex infections are unrelated and that genotyped loci do not exhibit significant linkage disequilibrium. Using the population minor allele frequency (MAF) of the genotyped loci, COIL uses the binomial distribution to estimate the likelihood of a COI level given the prevalence of observed monomorphic or polymorphic genotypes within each sample. COIL reliably estimates COI up to a level of three or five with at least 24 or 96 unlinked genotyped loci, respectively, as determined by in silico simulation and empirical validation. Evaluation of COI levels greater than five in patient samples may require a very large collection of genotype data, making sequencing a more cost-effective approach for evaluating COI under conditions when disease transmission is extremely high. Performance of the method is positively correlated with the MAF of the genotyped loci. COI estimates from existing SNP genotype datasets create a more detailed portrait of disease than analyses based simply on the number of polymorphic genotypes observed within samples. The capacity to reliably estimate COI from a genome-wide panel of SNP genotypes provides a potentially more accurate alternative to methods relying on PCR amplification of a small number of loci for estimating COI. This approach will also increase the number of applications of SNP genotype data, providing additional motivation to employ SNP barcodes for studies of disease epidemiology or control measure efficacy. The COIL program is available for download from GitHub, and users may also upload their SNP genotype data to a web interface for simple and efficient determination of sample COI

Spectral Imager of the Solar Atmosphere: The First Extreme-Ultraviolet Solar Integral Field Spectrograph Using Slicers

Particle acceleration, and the thermalisation of energetic particles, are fundamental processes across the universe. Whilst the Sun is an excellent object to study this phenomenon, since it is the most energetic particle accelerator in the Solar System, this phenomenon arises in many other astrophysical objects, such as active galactic nuclei, black holes, neutron stars, gamma ray bursts, solar and stellar coronae, accretion disks and planetary magnetospheres. Observations in the Extreme Ultraviolet (EUV) are essential for these studies but can only be made from space. Current spectrographs operating in the EUV use an entrance slit and cover the required field of view using a scanning mechanism. This results in a relatively slow image cadence in the order of minutes to capture inherently rapid and transient processes, and/or in the spectrograph slit ‘missing the action’. The application of image slicers for EUV integral field spectrographs is therefore revolutionary. The development of this technology will enable the observations of EUV spectra from an entire 2D field of view in seconds, over two orders of magnitude faster than what is currently possible. The Spectral Imaging of the Solar Atmosphere (SISA) instrument is the first integral field spectrograph proposed for observations at ∼180 Å combining the image slicer technology and curved diffraction gratings in a highly efficient and compact layout, while providing important spectroscopic diagnostics for the characterisation of solar coronal and flare plasmas. SISA’s characteristics, main challenges, and the on-going activities to enable the image slicer technology for EUV applications are presented in this paper

STIX X-ray microflare observations during the Solar Orbiter commissioning phase

Context. The Spectrometer/Telescope for Imaging X-rays (STIX) is the hard X-ray instrument onboard Solar Orbiter designed to observe solar flares over a broad range of flare sizes. Aims. We report the first STIX observations of solar microflares recorded during the instrument commissioning phase in order to investigate the STIX performance at its detection limit. Methods. STIX uses hard X-ray imaging spectroscopy in the range between 4-150 keV to diagnose the hottest flare plasma and related nonthermal electrons. This first result paper focuses on the temporal and spectral evolution of STIX microflares occuring in the Active Region (AR) AR12765 in June 2020, and compares the STIX measurements with Earth-orbiting observatories such as the X-ray Sensor of the Geostationary Operational Environmental Satellite (GOES/XRS), the Atmospheric Imaging Assembly of the Solar Dynamics Observatory, and the X-ray Telescope of the Hinode mission. Results. For the observed microflares of the GOES A and B class, the STIX peak time at lowest energies is located in the impulsive phase of the flares, well before the GOES peak time. Such a behavior can either be explained by the higher sensitivity of STIX to higher temperatures compared to GOES, or due to the existence of a nonthermal component reaching down to low energies. The interpretation is inconclusive due to limited counting statistics for all but the largest flare in our sample. For this largest flare, the low-energy peak time is clearly due to thermal emission, and the nonthermal component seen at higher energies occurs even earlier. This suggests that the classic thermal explanation might also be favored for the majority of the smaller flares. In combination with EUV and soft X-ray observations, STIX corroborates earlier findings that an isothermal assumption is of limited validity. Future diagnostic efforts should focus on multi-wavelength studies to derive differential emission measure distributions over a wide range of temperatures to accurately describe the energetics of solar flares. Conclusions. Commissioning observations confirm that STIX is working as designed. As a rule of thumb, STIX detects flares as small as the GOES A class. For flares above the GOES B class, detailed spectral and imaging analyses can be performed

Plasmodium chabaudi chabaudi malaria parasites can develop stable resistance to atovaquone with a mutation in the cytochrome b gene

<p>Abstract</p> <p>Background</p> <p><it>Plasmodium falciparum</it>, has developed resistance to many of the drugs in use. The recommended treatment policy is now to use drug combinations. The atovaquone-proguanil (AP) drug combination, is one of the treatment and prophylaxis options. Atovaquone (ATQ) exerts its action by inhibiting plasmodial mitochondria electron transport at the level of the cytochrome bc1 complex. <it>Plasmodium falciparum in vitro </it>resistance to ATQ has been associated with specific point mutations in the region spanning codons 271-284 of the <it>cytochrome b </it>gene. ATQ -resistant <it>Plasmodium yoelii </it>and <it>Plasmodium berghei </it>lines have been obtained and resistant lines have amino acid mutations in their CYT <it>b </it>protein sequences. <it>Plasmodium chabaudi </it>model for studying drug-responses and drug-resistance selection is a very useful rodent malaria model but no ATQ resistant parasites have been reported so far. The aim of this study was to determine the ATQ sensitivity of the <it>P. chabaudi </it>clones, to select a resistant parasite line and to perform genotypic characterization of the <it>cytb </it>gene of these clones.</p> <p>Methods</p> <p>To select for ATQ resistance, <it>Plasmodium. chabaudi chabaudi </it>clones were exposed to gradually increasing concentrations of ATQ during several consecutive passages in mice. <it>Plasmodium chabaudi cytb </it>gene was amplified and sequenced.</p> <p>Results</p> <p>ATQ resistance was selected from the clone AS-3CQ. In order to confirm whether an heritable genetic mutation underlies the response of AS-ATQ to ATQ, the stability of the drug resistance phenotype in this clone was evaluated by measuring drug responses after (i) multiple blood passages in the absence of the drug, (ii) freeze/thawing of parasites in liquid nitrogen and (iii) transmission through a mosquito host, <it>Anopheles stephensi</it>. ATQ resistance phenotype of the drug-selected parasite clone kept unaltered. Therefore, ATQ resistance in clone AS-ATQ is genetically encoded. The Minimum Curative Dose of AS-ATQ showed a six-fold increase in MCD to ATQ relative to AS-3CQ.</p> <p>Conclusions</p> <p>A mutation was found on the <it>P. chabaudi cytb </it>gene from the AS-ATQ sample a substitution at the residue Tyr268 for an Asn, this mutation is homologous to the one found in <it>P. falciparum </it>isolates resistant to ATQ.</p

Imported Plasmodium falciparum malaria in HIV-infected patients: a report of two cases

As HIV becomes a chronic infection, an increasing number of HIV-infected patients are travelling to malaria-endemic areas. Association of malaria with HIV/AIDS can be clinically severe. Severe falciparum malaria is a medical emergency that is associated with a high mortality, even when treated in an Intensive Care Unit. This article describes two cases of HIV-positive patients, who returned from malaria-endemic areas and presented a parasitaemia > 5% of erythrocytes and clinical signs of severe falciparum malaria, both with > 350 CD4 cell count/μl, absence of chemoprophylaxis and successful response. Factors like drug interactions and the possible implication of anti-malarial therapy bioavailability are all especially interesting in HIV-malaria co-infections

Confirmation of emergence of mutations associated with atovaquone-proguanil resistance in unexposed Plasmodium falciparum isolates from Africa

BACKGROUND: In vitro and in vivo resistance of Plasmodium falciparum to atovaquone or atovaquone-proguanil hydrochloride combination has been associated to two point mutations in the parasite cytochrome b (cytb) gene (Tyr268Ser and Tyr268Asn). However, little is known about the prevalence of codon-268 mutations in natural populations of P. falciparum without previous exposure to the drug in Africa. METHODS: The prevalence of codon-268 mutations in the cytb gene of African P. falciparum isolates from Nigeria, Malawi and Senegal, where atovaquone-proguanil has not been introduced for treatment of malaria was assessed. Genotyping of the cytb gene in isolates of P. falciparum was performed by PCR-restriction fragment length polymorphism and confirmed by sequencing. RESULTS: 295 samples from Nigeria (111), Malawi (91) and Senegal (93) were successfully analyzed for detection of either mutant Tyr268Ser or Tyr268Asn. No case of Ser268 or Asn268 was detected in cytb gene of parasites from Malawi or Senegal. However, Asn268 was detected in five out of 111 (4.5%) unexposed P. falciparum isolates from Nigeria. In addition, one out of these five mutant Asn268 isolates showed an additional cytb mutation leading to a Pro266Thr substitution inside the ubiquinone reduction site. CONCLUSION: No Tyr268Ser mutation is found in cytb of P. falciparum isolates from Nigeria, Malawi or Senegal. This study reports for the first time cytb Tyr268Asn mutation in unexposed P. falciparum isolates from Nigeria. The emergence in Africa of P. falciparum isolates with cytb Tyr268Asn mutation is a matter of serious concern. Continuous monitoring of atovaquone-proguanil resistant P. falciparum in Africa is warranted for the rational use of this new antimalarial drug, especially in non-immune travelers

Alpine ethnobotany in Italy: traditional knowledge of gastronomic and medicinal plants among the Occitans of the upper Varaita valley, Piedmont

A gastronomic and medical ethnobotanical study was conducted among the Occitan communities living in Blins/Bellino and Chianale, in the upper Val Varaita, in the Piedmontese Alps, North-Western Italy, and the traditional uses of 88 botanical taxa were recorded. Comparisons with and analysis of other ethnobotanical studies previously carried out in other Piemontese and surrounding areas, show that approximately one fourth of the botanical taxa quoted in this survey are also known in other surrounding Occitan valleys. It is also evident that traditional knowledge in the Varaita valley has been heavily eroded. This study also examined the local legal framework for the gathering of botanical taxa, and the potential utilization of the most quoted medicinal and food wild herbs in the local market, and suggests that the continuing widespread local collection from the wild of the aerial parts of Alpine wormwood for preparing liqueurs (Artemisia genipi, A. glacialis, and A. umbelliformis) should be seriously reconsidered in terms of sustainability, given the limited availability of these species, even though their collection is culturally salient in the entire study area