Temporal and spatial monitoring of mobile nanoparticles in a vineyard soil: Evidence of nanoaggregate formation

Mechanisms of formation, stabilization, liberation, transport and deposition of nanoparticles and their relationship to contaminant transport remain scarcely investigated in natural porous media. This study investigated nanoparticles mobilized in the pore space of a French vineyard soil by observing mobile soil-derived organic matter (SOM) and minerals in pore fluids over an 8-month monitoring period. Samples were collected in situ and investigated by transmission electron microscopy coupled to electron-dispersive spectroscopy. The main types of nanoparticles transported within the soil were clay, bacteria, SOM and nanoaggregates. Nanometric clay particles were enriched in various metals (Fe, Zn, As and Pb) and organically-derived constituents. Analyses of bacteria showed enrichments in Pb. SOM consisted of small carbon-based particles (\u3c200 nm) with slight enrichments in various metals. The fourth dominant particle type consisted of the association of particles forming organo-mineral nanoaggregates. Based on the study of more than 22 500 individual particles, we propose a schematic interpretation of the evolution of the distribution of particles with depth in a soil profile. The increase of nanoaggregates with depth in the soil seemed to be largely controlled by the ionic strength of soil water and soil hydrodynamics. Seasonal variations in temperature also appear to affect nanoaggregation. Based on the architecture of the nanoaggregates, we propose an improvement of pre-existing models of microaggregation by focusing on early aggregation stages suggesting the importance of bacteria and electrostatic interactions. The process of nanoaggregation can enhance the net reactivity of soil with respect to transported suspended matter, including heavy metals, and can initiate the process of C sequestration. © 2010 The Authors. Journal compilation © 2010 British Society of Soil Science

Bmcc1s, a Novel Brain-Isoform of Bmcc1, Affects Cell Morphology by Regulating MAP6/STOP Functions

The BCH (BNIP2 and Cdc42GAP Homology) domain-containing protein Bmcc1/Prune2 is highly enriched in the brain and is involved in the regulation of cytoskeleton dynamics and cell survival. However, the molecular mechanisms accounting for these functions are poorly defined. Here, we have identified Bmcc1s, a novel isoform of Bmcc1 predominantly expressed in the mouse brain. In primary cultures of astrocytes and neurons, Bmcc1s localized on intermediate filaments and microtubules and interacted directly with MAP6/STOP, a microtubule-binding protein responsible for microtubule cold stability. Bmcc1s overexpression inhibited MAP6-induced microtubule cold stability by displacing MAP6 away from microtubules. It also resulted in the formation of membrane protrusions for which MAP6 was a necessary cofactor of Bmcc1s. This study identifies Bmcc1s as a new MAP6 interacting protein able to modulate MAP6-induced microtubule cold stability. Moreover, it illustrates a novel mechanism by which Bmcc1 regulates cell morphology

Non-Photochemical Quenching in Cryptophyte Alga Rhodomonas salina Is Located in Chlorophyll a/c Antennae

Photosynthesis uses light as a source of energy but its excess can result in production of harmful oxygen radicals. To avoid any resulting damage, phototrophic organisms can employ a process known as non-photochemical quenching (NPQ), where excess light energy is safely dissipated as heat. The mechanism(s) of NPQ vary among different phototrophs. Here, we describe a new type of NPQ in the organism Rhodomonas salina, an alga belonging to the cryptophytes, part of the chromalveolate supergroup. Cryptophytes are exceptional among photosynthetic chromalveolates as they use both chlorophyll a/c proteins and phycobiliproteins for light harvesting. All our data demonstrates that NPQ in cryptophytes differs significantly from other chromalveolates – e.g. diatoms and it is also unique in comparison to NPQ in green algae and in higher plants: (1) there is no light induced xanthophyll cycle; (2) NPQ resembles the fast and flexible energetic quenching (qE) of higher plants, including its fast recovery; (3) a direct antennae protonation is involved in NPQ, similar to that found in higher plants. Further, fluorescence spectroscopy and biochemical characterization of isolated photosynthetic complexes suggest that NPQ in R. salina occurs in the chlorophyll a/c antennae but not in phycobiliproteins. All these results demonstrate that NPQ in cryptophytes represents a novel class of effective and flexible non-photochemical quenching

Conformational Altered p53 as an Early Marker of Oxidative Stress in Alzheimer's Disease

In order to study oxidative stress in peripheral cells of Alzheimer's disease (AD) patients, immortalized lymphocytes derived from two peculiar cohorts of patients, referring to early onset AD (EOSAD) and subjects harboured AD related mutation (ADmut), were used. Oxidative stress was evaluated measuring i) the typical oxidative markers, such as HNE Michel adducts, 3 Nitro-Tyrosine residues and protein carbonyl on protein extracts, ii) and the antioxidant capacity, following the enzymatic kinetic of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRD). We found that the signs of oxidative stress, measured as oxidative marker levels, were evident only in ADmut but not in EOSAD patients. However, oxidative imbalance in EOSAD as well as ADmut lymphocytes was underlined by a reduced SOD activity and GRD activity in both pathological groups in comparison with cells derived from healthy subjects. Furthermore, a redox modulated p53 protein was found conformational altered in both EOSAD and ADmut B lymphocytes in comparison with control cells. This conformational altered p53 isoform, named “unfolded p53”, was recognized by the use of two specific conformational anti-p53 antibodies. Immunoprecipitation experiments, performed with the monoclonal antibodies PAb1620 (that recognizes p53wt) and PAb240 (that is direct towards unfolded p53), and followed by the immunoblotting with anti-4-hydroxynonenal (HNE) and anti- 3-nitrotyrosine (3NT) antibodies, showed a preferential increase of nitrated tyrosine residues in unfolded p53 isoform comparing to p53 wt protein, in both ADmut and EOSAD. In addition, a correlation between unfolded p53 and SOD activity was further found. Thus this study suggests that ROS/RNS contributed to change of p53 tertiary structure and that unfolded p53 can be considered as an early marker of oxidative imbalance in these patients

In vitro nuclear interactome of the HIV-1 Tat protein

<p>Abstract</p> <p>Background</p> <p>One facet of the complexity underlying the biology of HIV-1 resides not only in its limited number of viral proteins, but in the extensive repertoire of cellular proteins they interact with and their higher-order assembly. HIV-1 encodes the regulatory protein Tat (86–101aa), which is essential for HIV-1 replication and primarily orchestrates HIV-1 provirus transcriptional regulation. Previous studies have demonstrated that Tat function is highly dependent on specific interactions with a range of cellular proteins. However they can only partially account for the intricate molecular mechanisms underlying the dynamics of proviral gene expression. To obtain a comprehensive nuclear interaction map of Tat in T-cells, we have designed a proteomic strategy based on affinity chromatography coupled with mass spectrometry.</p> <p>Results</p> <p>Our approach resulted in the identification of a total of 183 candidates as Tat nuclear partners, 90% of which have not been previously characterised. Subsequently we applied <it>in silico </it>analysis, to validate and characterise our dataset which revealed that the Tat nuclear interactome exhibits unique signature(s). First, motif composition analysis highlighted that our dataset is enriched for domains mediating protein, RNA and DNA interactions, and helicase and ATPase activities. Secondly, functional classification and network reconstruction clearly depicted Tat as a polyvalent protein adaptor and positioned Tat at the nexus of a densely interconnected interaction network involved in a range of biological processes which included gene expression regulation, RNA biogenesis, chromatin structure, chromosome organisation, DNA replication and nuclear architecture.</p> <p>Conclusion</p> <p>We have completed the <it>in vitro </it>Tat nuclear interactome and have highlighted its modular network properties and particularly those involved in the coordination of gene expression by Tat. Ultimately, the highly specialised set of molecular interactions identified will provide a framework to further advance our understanding of the mechanisms of HIV-1 proviral gene silencing and activation.</p

Protective intraoperative ventilation with higher versus lower levels of positive end-expiratory pressure in obese patients (PROBESE): study protocol for a randomized controlled trial

Background: Postoperative pulmonary complications (PPCs) increase the morbidity and mortality of surgery in obese patients. High levels of positive end-expiratory pressure (PEEP) with lung recruitment maneuvers may improve intraoperative respiratory function, but they can also compromise hemodynamics, and the effects on PPCs are uncertain. We hypothesized that intraoperative mechanical ventilation using high PEEP with periodic recruitment maneuvers, as compared with low PEEP without recruitment maneuvers, prevents PPCs in obese patients. Methods/design The PRotective Ventilation with Higher versus Lower PEEP during General Anesthesia for Surgery in OBESE Patients (PROBESE) study is a multicenter, two-arm, international randomized controlled trial. In total, 2013 obese patients with body mass index ≥35 kg/m2 scheduled for at least 2 h of surgery under general anesthesia and at intermediate to high risk for PPCs will be included. Patients are ventilated intraoperatively with a low tidal volume of 7 ml/kg (predicted body weight) and randomly assigned to PEEP of 12 cmH2O with lung recruitment maneuvers (high PEEP) or PEEP of 4 cmH2O without recruitment maneuvers (low PEEP). The occurrence of PPCs will be recorded as collapsed composite of single adverse pulmonary events and represents the primary endpoint. Discussion To our knowledge, the PROBESE trial is the first multicenter, international randomized controlled trial to compare the effects of two different levels of intraoperative PEEP during protective low tidal volume ventilation on PPCs in obese patients. The results of the PROBESE trial will support anesthesiologists in their decision to choose a certain PEEP level during general anesthesia for surgery in obese patients in an attempt to prevent PPCs. Trial registration ClinicalTrials.gov identifier: NCT02148692. Registered on 23 May 2014; last updated 7 June 2016. Electronic supplementary material The online version of this article (doi:10.1186/s13063-017-1929-0) contains supplementary material, which is available to authorized users

Résultats d'essais du cyclotron de grenoble

The internal beam acceptance of the Grenoble Cyclotron was successfully tested in october 1967. Then after assembly of the extraction system in the machine, acceptance on a proton extracted beam was analysed in july 1968 at an energy 50 MeV. The extraction efficiency was often as high as 70 % between the internal probe and the beam exit port. This high efficiency comes from using the first harmonic of the magnetic field. Other tests were performed at various energies (19, 30, 40 MeV) and for different particles (α) . These show the possibilities of this machine for the easy extraction of beams of different particles at various energies.Les essais de réception en faisceau interne du Cyclotron de Grenoble ont eu lieu en octobre 1967 et ont été couronnés de succès ; le système d'extraction a ensuite été monté dans la machine et les essais de réception du cyclotron en faisceau extrait (protons) ont eu lieu en juillet 1968. L'énergie du faisceau était de 50 MeV. Le rendement d'extraction du cyclotron atteint couramment 70 % entre sonde interne et fenêtre de sortie du faisceau ; ce haut rendement étant dû à l'emploi de l'harmonique 1 de champ magnétique. D'autres essais ont eu lieu à d'autres énergies (40, 30, 19 MeV) et pour d'autres particules (α) montrant ainsi les possibilités de cette machine quant à la facilité d'extraction aux différentes énergies et pour différentes particules

Résultats d'essais du cyclotron de grenoble

Les essais de réception en faisceau interne du Cyclotron de Grenoble ont eu lieu en octobre 1967 et ont été couronnés de succès ; le système d'extraction a ensuite été monté dans la machine et les essais de réception du cyclotron en faisceau extrait (protons) ont eu lieu en juillet 1968. L'énergie du faisceau était de 50 MeV. Le rendement d'extraction du cyclotron atteint couramment 70 % entre sonde interne et fenêtre de sortie du faisceau ; ce haut rendement étant dû à l'emploi de l'harmonique 1 de champ magnétique. D'autres essais ont eu lieu à d'autres énergies (40, 30, 19 MeV) et pour d'autres particules (α) montrant ainsi les possibilités de cette machine quant à la facilité d'extraction aux différentes énergies et pour différentes particules