INFOGRAPHIC MODELING BASED ON 3D LASER SURVEYING FOR INFORMED UNIVERSAL DESIGN IN ARCHAEOLOGICAL AREAS: THE CASE OF OPPIDUM OF THE ANCIENT CITY OF TUSCULUM

The valorisation of archaeological sites represents a fundamental action for the social and economic development of a country. An archaeological park is often a territory characterized by significant testimonies of antiquity of great landscape value. For this reason, it should be configured as an authentic outdoor museum, enriched by natural, environmental, architectural and urban components. In order to fulfill these requirements, it is fundamental the elaboration of a coherent scientific project of preservation, fruition and valorisation of the area, which merge the different components necessary for the establishment of an archaeological museum-park. One of the most critical aspects related to the fruition of archaeological sites is the accessibility to areas and routes, not always – if ever – designed for people with reduced mobility, also temporary (for example elderly, obese, visually impaired, etc.). In general, an established principle used in the new design is to pay attention to the so-called wide users, in accordance with the international guidelines summarized in the concept of Universal Design. In particular, this paper presents the use of three-dimensional models obtained from laser scanning surveys for the design of walking trails for people with reduced mobility in the Tusculum Archaeological-Cultural Park. The work was based on the fundamental implementation of the three-dimensional survey with terrestrial laser scanning for the construction and the control of the complex morphology of the site, and on the subsequent integration of models of the intervention in the three-dimensional reality "as-built" of the site. The obtained infographic model allowed to study and simulate the impact of the routes for people with reduced mobility, and to verify its efficiency in the historical and landscape context. Moreover, it was possible to verify the construction of other facilities in the real conditions of the site

Fermentation of Vaccinium floribundum Berries with Lactiplantibacillus plantarum Reduces Oxidative Stress in Endothelial Cells and Modulates Macrophages Function

Accumulating evidence suggests that high consumption of natural antioxidants promotes health by reducing oxidative stress and, thus, the risk of developing cardiovascular diseases. Similarly, fermentation of natural compounds with lactic acid bacteria (LAB), such as Lactiplantibacillus plantarum, enhances their beneficial properties as regulators of the immune, digestive, and cardiovascular system. We investigated the effects of fermentation with Lactiplantibacillus plantarum on the antioxidant and immunomodulatory effects of Pushgay berries (Vaccinium floribundum, Ericaceae family) in human umbilical vein endothelial cells (HUVECs) and macrophage cell line RAW264.7. Polyphenol content was assayed by Folin-Ciocalteu and HPLC-MS/MS analysis. The effects of berries solutions on cell viability or proliferation were assessed by WST8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt and Lactate dehydrogenase (LDH) release, Trypan blue exclusion test, and Alamar blue assay. Antioxidant activity was evaluated by a cell-based chemiluminescent probe for the detection of intracellular H2O2 production in HUVECs. Heme oxygenase-1 (HO-1) expression levels were investigated by RT-qPCR. Glutathione reductase (GR), glutathione peroxidase (Gpx), superoxide dismutase (SOD), and catalase (CAT) activities, as markers of intracellular antioxidant defense, were evaluated by spectrophotometric analysis. The immunomodulatory activity was examined in RAW 264.7 by quantification of inducible nitric oxide synthase (iNOS) and Tumor Necrosis Factor-alpha (TNF alpha) by RT-qPCR. Data showed that fermentation of Pushgay berries (i) enhances the content of quercetin aglycone, and (ii) increases their intracellular antioxidant activity, as indicated by the reduction in H2O2-induced cell death and the decrease in H2O2-induced HO-1 gene expression in HUVECs treated for 24 h with fermented berries solution (10 mu g/mL). Moreover, treatment with Pushgay berries for 72 h (10 mu g/mL) promotes cells growth in RAW 264.7, and only fermented Pushgay berries increase the expression of iNOS in the same cell line. Taken together, our results show that LAB fermentation of Pushgay berries enhances their antioxidant and immunomodulatory properties

Invasive fungal diseases in haematopoietic cell transplant recipients and in patients with acute myeloid leukaemia or myelodysplasia in Brazil

AbstractInvasive fungal disease (IFD) shows distinct regional incidence patterns and epidemiological features depending on the geographic region. We conducted a prospective survey in eight centres in Brazil from May 2007 to July 2009. All haematopoietic cell transplant (HCT) recipients and patients with acute myeloid leukaemia (AML) or myelodysplasia (MDS) were followed from admission until 1 year (HCT) or end of consolidation therapy (AML/MDS). The 12-month cumulative incidence (CI) of proven or probable IFD was calculated, and curves were compared using the Grey test. Among 237 AML/MDS patients and 700 HCT recipients (378 allogeneic, 322 autologous), the 1-year CI of IFD in AML/MDS, allogeneic HCT and autologous HCT was 18.7%, 11.3% and 1.9% (p <0.001), respectively. Fusariosis (23 episodes), aspergillosis (20 episodes) and candidiasis (11 episodes) were the most frequent IFD. The 1-year CI of aspergillosis and fusariosis in AML/MDS, allogeneic HCT and autologous HCT were 13.4%, 2.3% and 0% (p <0.001), and 5.2%, 3.8% and 0.6% (p 0.01), respectively. The 6-week probability of survival was 53%, and was lower in cases of fusariosis (41%). We observed a high burden of IFD and a high incidence and mortality for fusariosis in this first multicentre epidemiological study of IFD in haematological patients in Brazil

A SURVEY of CHRONIC GVHD and OTHER OUTCOMES - A SNAPSHOT of BRAZILIAN ALLOGENEIC HEMATOPOIETIC CELL TRANSPLANTATION (HCT) CENTERS

Univ Fed Parana, BR-80060000 Curitiba, Parana, BrazilNatl Canc Inst, Rio de Janeiro, BrazilState Univ São Paulo, São Paulo, BrazilUniv Estadual Campinas, Campina, BrazilUniv Fed Rio de Janeiro, Rio de Janeiro, BrazilAmaral Carvalho Hosp, Jau, BrazilAmaral Carvalho Hosp, São Paulo, BrazilUniversidade Federal de São Paulo, São Paulo, BrazilAlbert Einstein Hosp, São Paulo, BrazilCanc Hosp Barretos, São Paulo, BrazilUniv Fed Rio Grande do Sul, Porto Alegre, RS, BrazilPernambuco Blood Bank, Recife, PE, BrazilUniv Fed Minas Gerais, Belo Horizonte, MG, BrazilUniv State Univ São Paulo, Ribeirao Preto, BrazilFred Hutchinson Canc Res Ctr, Seattle, WA 98104 USAUniversidade Federal de São Paulo, São Paulo, BrazilWeb of Scienc

Anales de Edafología y Agrobiología Tomo 36 Número 1-2

Duración efectiva del índice de sequedad, por M. P. Garmendía y J. Garmendía.-- Adsorción y evolución de manganeso en arcillas, por O. Carpena, I. Tovar, A. Lax y F. Costa.-- Ecología de leguminosas en relación con algunos factores ambientales en Guadalajara. I. Aspectos florísticos y relación con la clase ele suelo, por M. Morey.-- Variaciones del contenido de nitrógeno en una plantación de Lolium perenne,por Esther Simón Martínez.-- Predicción de temperaturas máximas diarias, por E. Hernández, J. A. Hernández, J. F. Sánchez y J. Garmendía.-- Efectos del almacenaje sobre las propiedades físicas y biológicas de muestras tamizadas de suelos orgánicos, por F. Díaz-Fierros Viqueiro.-- Morfometría del cuarzo y circón aplicada al estudio genético de un suelo policíclico, por M. C. Villar Celorio.-- Contribución al estudio de la terra rossa española. II. Mineralogía de la fracción arcilla, por L. J. Alías, M. Nieto y J. Albaladejo.-- Entisoles del Campo de Cartagena (Murcia). Características generales y mineralógicas, por L. J. Alías y R. Ortiz Silla.-- Estudio sobre la composición química de variedades de almendra del sureste español, por F. Romojaro, J. F. García y F. J. López Andreu.-- Componentes del plátano canario y sus variaciones durante la maduración, por A. Carlos Blesa, M. A. Rodríguez Raymond y A. Maestre.-- Contribución al estudio de la platanera canaria. Relación entre la actividad respiratoria y la maduración de los plátanos, por A. Carlos Blesa, M. A Rodríguez Raymoud, C. D. Lorenzo e Isabel López.-- Notas.-- Reestructuración del C. S. I. C.-- Nombramiento del Prof. Casas Peláez como Presidente del C. S. I. C. 1.-- Carta del Presidente del C. S. I. C. al personal del mismo.-- Nombramiento del Prof. Snárez y Suárez como Director general de Educación Bastea.-- 6.° Curso Internacional de Fertilidad de Suelos y Nutrición Vegetal.—8ª Reunión Internacional de Micromorfología de Suelos.—19ª Conferencia General de la UNESCO.-- Sociedad Española de Ciencia del Suelo.-- Nombramiento de Secretario del Centro de Edafología y Biología Aplicada de Salamanca.-- Dimisión del Director del Centre de Edafología y Biología Aplicada del Cuarto (Sevilla).-- Propuesta de Director del Centro de Edafología y Biología Aplicada del Cuarto.-- Viaje del Prof. Troncoso.-- Grupo Español de Trabajo del Cuaternario.-- Autorizaciones para realizar función docente.-- Invitaciones a Profesores extranjeros.-- Programa de cooperación internacional con lberoamérica: bolsas de estudio y viaje .-- Conferencia del Prof. Salerno.-- Viaje realizado a Hispanoamérica por el Dr. D. Francisco Girela Vilchez.-- Creación del Centro de Formaci.ón y Promoción de Personal del C. S. I. C. (C. F. P. P.)Peer reviewed2019-08.- CopyBook.- Libnova.- Biblioteca ICA

Substrate specificity of the TRAMP nuclear surveillance complexes

During nuclear surveillance in yeast and human cells, the RNA exosome functions together with the TRAMP complexes. Here, the authors defined the protein composition of the TRAMP complexes and identified specific RNA binding sites for the different TRAMP components

Safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: SETOPROB study

We previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036. These strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low pH. In the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy volunteers in three Spanish cities was carried out to evaluate the tolerance, safety, gut colonization and immunomodulatory effects of these three probiotics. Volunteers underwent a 15-day washout period, after which they were randomly divided into 5 groups that received daily a placebo, a capsule containing one of the 3 strains or a capsule containing a mixture of two strains for 30 days. The intervention was followed by another 15-day washout period. Patients did not consume fermented milk for the entire duration of the study. Gastrointestinal symptoms, defecation frequency and stool consistency were not altered by probiotic intake. No relevant changes in blood and serum, as well as no adverse events occurred during or after treatment. Probiotic administration slightly modified bacterial populations in the volunteers’ feces. Intestinal persistence occurred in volunteers who received L. rhamnosus CNCM I-4036. Administration of B. breve CNCM I-4035 resulted in a significant increase in fecal secretory IgA content. IL-4 and IL-10 increased, whereas IL-12 decreased in the serum of volunteers treated with any of the three strains. These results demonstrate that the consumption of these three bacterial strains was safe and exerted varying degrees of immunomodulatory effects.Part of the research currently in progress in the authors' laboratory is funded by the company Hero Spain, S. A. through the grant #3582 managed by the Fundacion General Empresa-Universidad de Granada