1,766 research outputs found
Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro
Microtubule structure and functions have been widely studied in vitro and in
cells. Research has shown that cysteines on tubulin play a crucial role in the
polymerization of microtubules. Here, we show that blocking sulfhydryl groups
of cysteines in taxol-stabilized polymerized microtubules with a commonly used
chemical crosslinker prevents temporal end-to-end annealing of microtubules in
vitro. This can dramatically affect the length distribution of the
microtubules. The crosslinker sulfosuccinimidyl
4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a
maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups
and primary amines, respectively. Interestingly, addition of a maleimide dye
alone does not show the same interference with annealing in stabilized
microtubules. This study shows that the sulfhydryl groups of cysteines of
tubulin that are vital for the polymerization are also important for the
subsequent annealing of microtubules.Comment: 3 figure
Does The Girl You Left Behind Ever Wish You Back Again? : Waltz Song
https://digitalcommons.library.umaine.edu/mmb-vp/5632/thumbnail.jp
When Darling Bess First Whispered Yes
https://digitalcommons.library.umaine.edu/mmb-vp/4824/thumbnail.jp
The Girl I Loved In Auld Lang Syne
https://digitalcommons.library.umaine.edu/mmb-vp/4202/thumbnail.jp
When The Springtime Brings The Roses, Jessie Dear
https://digitalcommons.library.umaine.edu/mmb-vp/5058/thumbnail.jp
My Heart\u27s To-Night In Texas : By The Silvery Rio Grande
https://digitalcommons.library.umaine.edu/mmb-vp/2184/thumbnail.jp
When the Evening Bells Are Chiming Songs of Auld Lang Syne
https://digitalcommons.library.umaine.edu/mmb-vp/5132/thumbnail.jp
When It\u27s Moonlight On The Prairie : There\u27s A Parson Only Twenty Miles Away
https://digitalcommons.library.umaine.edu/mmb-vp/5012/thumbnail.jp
I Never Believed In Angels Until I Met You : Girl Of The Cross
https://digitalcommons.library.umaine.edu/mmb-vp/5244/thumbnail.jp
Daddy\u27s Little Tom Boy Girl
https://digitalcommons.library.umaine.edu/mmb-vp/4958/thumbnail.jp
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