Revisiting core issues in dynamic assessment

Dynamic assessment is currently poised at a juncture at which theoretical and practical assessment resolutions are necessitated. Such issues concern theoretical approaches towards  psycho-educational assessment.  In order to partially explore these basic assessment approaches, a questionnaire was delivered via electronic mail to one hundred internationally, currently active dynamic assessment researchers and practitioners. The findings from the responses formed the basis for an informal content analysis, which was conducted utilising themes as primary meaning unit and word counts as secondary meaning unit of analyses. The one common and uniting feature about the current research in this area is the broad range of theoretical approaches towards assessment and the current lack of unanimity across types of approaches. Responses showed that varied theoretical frameworks are employed in dynamic assessments which do not necessarily cohere with other traditional approaches. It is contended that an exploratory revisiting of core assessment approaches would assist in positioning practitioners’ and researchers’ theoretical approaches in future assessments

A review of South African research in the field of dynamic assessment

Dynamic assessment, which is often characterised by the learning potential approach across the world and in South Africa, is receiving more attention from educators and research practitioners alike. When compared to the status of international research, local dynamic assessment research can still be regarded as being in its infancy. A selection of studies conducted within this domain was analysed and the results carefully assessed in terms of positive and negative findings to serve as an indication of the trends that this discipline may face in South Africa. The main findings indicate that although the field is still being researched today, there has been a decrease in the number of studies as well as a concomitant decrease in the implementation of dynamic research efforts. The reasons cited are a lack of time, costs, inefficiencies and also confusion as to what dynamic assessment entails. There is, as yet, no consistent definition of dynamic assessment in South Africa, which makes it all the harder to entrench dynamic assessment as a methodology and implement it on as wide a scale as possible

Application of magnesium hydroxide and barium hydroxide for the removal of metals and sulphate from mine water

The proposed magnesium-barium-oxide process consists of metal removal with Mg(OH)2, magnesium and sulphate removal with Ba(OH)2 and calcium removal with CO2. The raw materials, Mg(OH)2 and Ba(OH)2 are recovered from the BaSO4 and Mg(OH)2 sludges that are produced. Laboratory studies showed that metals are removed to low levels. This includes iron(II), the dominant metal ion in mine water, which is first oxidised to iron(III), whereafter it precipitates as Fe(OH)3 resulting in residual levels of Fe(II) in the mine water of less than 20 mg/ℓ. Sulphate is also removed to less than 25 mg/ℓ. The final sulphate concentration is a function of the amount of Ba(OH)2 dosed, as the amount of sulphate removed is stoichiometrically equivalent to the Ba(OH)2 dosage. During CO2-dosing, CaCO3 is precipitated to the saturation level of CaCO3.Keywords: Magnesium hydroxide; barium hydroxide; sulphate removal; water treatmen

PCOC National Report on Outcomes in Palliative Care in Australia July to December 2011

The Palliative Care Outcomes Collaboration (PCOC) was established in mid-2005 and is funded under the National Palliative Care Program supported by the Australian Government Department of Health and Ageing. The goal of the PCOC is to use standardised, validated, clinical assessment tools to benchmark and measure outcomes in palliative care; and assist palliative care services to improve the quality of care. Further information on the tools can be found at www.pcoc.org.au. Each service involved in PCOC submits data every six months. The data are then collated and fed back to services to inform service improvement. Participation in PCOC is voluntary. There are three levels of data items - patient, episode and phase. The broad detail is found at the patient level, where the data items look at patient demographics. At the episode level, the items focus on characterising each setting of palliative care. They also describe the reasons behind why and how palliative care episodes start/end, the level of support patients receive both before and after an episode and (where applicable) the setting in which the patient died. The clinical focus of the data is at the phase level. The items at this level describe the patient\u27s stage of illness, functional impairment as well as their levels of pain and symptom distress. The items at the phase level are used to quantify patient outcomes, and are the focus of the PCOC benchmarks. The current PCOC data set (Version 2) was introduced in July 2007 following consultation with palliative care services and approval by PCOC\u27s Scientific and Clinical Advisory Committee. The data set includes five clinical assessment tools: Phases of Care, Palliative Care Problem Severity Score (PCPSS), Symptom Assessment Scale (SAS), Australia-modified Karnofsky Performance Status Scale (AKPS) and Resource Utilisation Groups - Activities of Daily Living (RUG-ADL). The items included in the PCOC data set serve the dual purpose of: - defining a common clinical language to allow communication between palliative care providers - facilitating the routine collection of national palliative care data for the purpose of reporting and benchmarking to drive quality improvement. Revised phase definitions were implemented in January 2012 but the data in this report does not reflect the revised definitions

Cell adhesion and cortex contractility determine cell patterning in the Drosophila retina

Hayashi and Carthew (Nature 431 [2004], 647) have shown that the packing of cone cells in the Drosophila retina resembles soap bubble packing, and that changing E- and N-cadherin expression can change this packing, as well as cell shape. The analogy with bubbles suggests that cell packing is driven by surface minimization. We find that this assumption is insufficient to model the experimentally observed shapes and packing of the cells based on their cadherin expression. We then consider a model in which adhesion leads to a surface increase, balanced by cell cortex contraction. Using the experimentally observed distributions of E- and N-cadherin, we simulate the packing and cell shapes in the wildtype eye. Furthermore, by changing only the corresponding parameters, this model can describe the mutants with different numbers of cells, or changes in cadherin expression.Comment: revised manuscript; 8 pages, 6 figures; supplementary information not include

Grapevine leafroll-associated virus 3.

Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a general overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classification of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional serological assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics. The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management

Patient Outcomes in Palliative Care, Report 13 (January - June 2012) - Western Australia

PCOC aims to assist services to improve the quality of the palliative care they provide through the analysis and benchmarking of patient outcomes. In this, the thirteenth PCOC report, data submitted for the January - June 2012 period are summarised and patient outcomes benchmarked to enable participating services to assess their performance and identify areas in which they may improve. This report is broken into four sections: Section 1 provides a summary of the data included in this report. Section 2 summarises each of the four outcome measures and presents national benchmarking results for a selection of these measures. Section 3 presents a more detailed analysis of the outcome measures and benchmarks. Section 4 provides descriptive analysis at each of the patient, episode and phase data levels. In each of the four sections, data and analysis for services in WA is presented alongside the national figures for comparative purposes. The national figures reflect all palliative care services who submitted data for the January - June 2012 period. A full list of these services can be found at www.pcoc.org.au The four outcome measures included in this report were first introduced in the reporting period January to June 2009 (Report 7). There is strong sectoral support for national benchmarks and a consensus that such benchmarks can drive service innovation regardless of model of care. Benchmarking provides opportunities to understand the services that are provided, the outcomes patients experience and also to generate research opportunities focused on how to demonstrate variations in practice and outcomes

BDE-209 in the Australian environment: desktop review

The commercial polybrominated diphenyl ether (PBDE) flame retardant mixture c-decaBDE is now being considered for listing on the Stockholm Convention on Persistent Organic Pollutants. The aim of our study was to review the literature regarding the use and detection of BDE-209, a major component of c-decaBDE, in consumer products and provide a best estimate of goods that are likely to contain BDE-209 in Australia. This review is part of a larger study, which will include quantitative testing of items to assess for BDE-209. The findings of this desktop review will be used to determine which items should be prioritized for quantitative testing. We identified that electronics, particularly televisions, computers, small household appliances and power boards, were the items that were most likely to contain BDE-209 in Australia. Further testing of these items should include items of various ages. Several other items were identified as high priority for future testing, including transport vehicles, building materials and textiles in non-domestic settings. The findings from this study will aid in the development of appropriate policies, should listing of c-decaBDE on the Stockholm Convention and Australia's ratification of that listing proceed

Transmission of Foot-and-Mouth Disease SAT2 Viruses at the Wildlife-Livestock Interface of Two Major Transfrontier Conservation Areas in Southern Africa.

Over a decade ago, foot-and-mouth disease (FMD) re-emerged in Southern Africa specifically in beef exporting countries that had successfully maintained disease-free areas in the past. FMD virus (FMDV) serotype SAT2 has been responsible for a majority of these outbreaks. Epidemiological studies have revealed the importance of the African buffalo as the major wildlife FMD reservoir in the region. We used phylogeographic analysis to study dynamics of FMD transmission between buffalo and domestic cattle at the interface of the major wildlife protected areas in the region currently encompassing two largest Transfrontier conservation areas: Kavango-Zambezi (KAZA) and Great Limpopo (GL). Results of this study showed restricted local occurrence of each FMDV SAT2 topotypes I, II, and III, with occasional virus migration from KAZA to GL. Origins of outbreaks in livestock are frequently attributed to wild buffalo, but our results suggest that transmission from cattle to buffalo also occurs. We used coalescent Bayesian skyline analysis to study the genetic variation of the virus in cattle and buffalo, and discussed the association of these genetic changes in the virus and relevant epidemiological events that occurred in this area. Our results show that the genetic diversity of FMDV SAT2 has decreased in buffalo and cattle population during the last decade. This study contributes to understand the major dynamics of transmission and genetic variation of FMDV SAT2 in Southern Africa, which will could ultimately help in designing efficient strategies for the control of FMD at a local and regional level

An improved liquid chromatography tandem mass spectrometry (LC-MS/MS) method for quantification of dexmedetomidine concentrations in samples of human plasma

Dexmedetomidine (DMET) is a sedative, analgesic and anxiolytic with minimum adverse respiratory effects. An LC-MS/MS bioanalytical method has been developed and validated to accurately measure DMET concentrations in samples of human plasma. The method overcomes difficulties in the extraction and quantification of DMET due to the fact that it binds strongly to glass and plastic tubes, as well as solid phase extraction (SPE) cartridges. Human plasma (50 μL) was mixed with the internal standard (IS) (DMET-d4) solution (100 μL) and 0.1% formic acid (50 μL) and extracted using Oasis HLB 1 CC (30 mg) solid phase extraction (SPE) cartridges (Waters®). The glass tubes were coated with bovine serum albumin (BSA) 0.5% (20 μL) before eluting DMET and the IS. After evaporation under nitrogen at room temperature, the analytes were reconstituted in 20% acetonitrile in 0.1% formic acid in water and transferred to silanized glass vials. An electrospray ionisation (ESI) mass spectrometry method in positive mode was created and the precursor/product transitions (m/z) were 201.1 → 95.0 (DMET) and 204.9 → 99.0 (IS). The method was robust and fully validated based on the 2012 EMEA guideline for bioanalytical method validation in the concentration range of 0.5-20 ng/mL. Using this assay, we showed that DMET binds strongly to Extracorporeal Membrane Oxygenation (ECMO) circuits, consistent with expectations for small lipophilic compounds