63 research outputs found

    Radio-Frequency Spectroscopy

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    Contains reports on three research projects

    Radio-Frequency Spectroscopy

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    Contains reports on three research projects

    Radio-Frequency Spectroscopy

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    Contains reports on four research projects

    Radio-Frequency Spectroscopy

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    Contains reports on four research projects

    Osteoinduction in human fat derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli

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    Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity
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