1,502 research outputs found
Super-resolution provided by the arbitrarily strong superlinearity of the blackbody radiation
Blackbody radiation is a fundamental phenomenon in nature, and its explanation by Planck marks a cornerstone in the history of Physics. In this theoretical work, we show that the spectral radiance given by Planck's law is strongly superlinear with temperature, with an arbitrarily large local exponent for decreasing wavelengths. From that scaling analysis, we propose a new concept of super-resolved detection and imaging: if a focused beam of energy is scanned over an object that absorbs and linearly converts that energy into heat, a highly nonlinear thermal radiation response is generated, and its point spread function can be made arbitrarily smaller than the excitation beam focus. Based on a few practical scenarios, we propose to extend the notion of super-resolution beyond its current niche in microscopy to various kinds of excitation beams, a wide range of spatial scales, and a broader diversity of target objects
Ultrastructural anatomy of nodes of Ranvier in the peripheral nervous system as revealed by STED microscopy.
We used stimulated emission depletion (STED) superresolution microscopy to analyze the nanoscale organization of 12 glial and axonal proteins at the nodes of Ranvier of teased sciatic nerve fibers. Cytoskeletal proteins of the axon (betaIV spectrin, ankyrin G) exhibit a high degree of one-dimensional longitudinal order at nodal gaps. In contrast, axonal and glial nodal adhesion molecules [neurofascin-186, neuron glial-related cell adhesion molecule (NrCAM)] can arrange in a more complex, 2D hexagonal-like lattice but still feature a âź190-nm periodicity. Such a lattice-like organization is also found for glial actin. Sodium and potassium channels exhibit a one-dimensional periodicity, with the Nav channels appearing to have a lower degree of organization. At paranodes, both axonal proteins (betaII spectrin, Caspr) and glial proteins (neurofascin-155, ankyrin B) form periodic quasiâone-dimensional arrangements, with a high degree of interdependence between the position of the axonal and the glial proteins. The results indicate the presence of mechanisms that finely align the cytoskeleton of the axon with the one of the Schwann cells, both at paranodal junctions (with myelin loops) and at nodal gaps (with microvilli). Taken together, our observations reveal the importance of the lateral organization of proteins at the nodes of Ranvier and pave the way for deeper investigations of the molecular ultrastructural mechanisms involved in action potential propagation, the formation of the nodes, axonâglia interactions, and demyelination diseases
Ultrafast spectroscopy of single molecules
We present a single-molecule study on femtosecond dynamics in multichromophoric systems, combining fs pump-probe, emission-spectra and fluorescence-lifetime analysis. At the single molecule level a wide range of exciton delocalisation lengths and energy redistribution times is revealed. Next, two color pump-probe experiments are presented as a step to addressing ultrafast energy transfer in individual complexes
Dynamic L-type CaV1.2 channel trafficking facilitates CaV1.2 clustering and cooperative gating.
L-type CaV1.2 channels are key regulators of gene expression, cell excitability and muscle contraction. CaV1.2 channels organize in clusters throughout the plasma membrane. This channel organization has been suggested to contribute to the concerted activation of adjacent CaV1.2 channels (e.g. cooperative gating). Here, we tested the hypothesis that dynamic intracellular and perimembrane trafficking of CaV1.2 channels is critical for formation and dissolution of functional channel clusters mediating cooperative gating. We found that CaV1.2 moves in vesicular structures of circular and tubular shape with diverse intracellular and submembrane trafficking patterns. Both microtubules and actin filaments are required for dynamic movement of CaV1.2 vesicles. These vesicles undergo constitutive homotypic fusion and fission events that sustain CaV1.2 clustering, channel activity and cooperative gating. Our study suggests that CaV1.2 clusters and activity can be modulated by diverse and unique intracellular and perimembrane vesicular dynamics to fine-tune Ca2+ signals
Lower Bounds for the Graph Homomorphism Problem
The graph homomorphism problem (HOM) asks whether the vertices of a given
-vertex graph can be mapped to the vertices of a given -vertex graph
such that each edge of is mapped to an edge of . The problem
generalizes the graph coloring problem and at the same time can be viewed as a
special case of the -CSP problem. In this paper, we prove several lower
bound for HOM under the Exponential Time Hypothesis (ETH) assumption. The main
result is a lower bound .
This rules out the existence of a single-exponential algorithm and shows that
the trivial upper bound is almost asymptotically
tight.
We also investigate what properties of graphs and make it difficult
to solve HOM. An easy observation is that an upper
bound can be improved to where
is the minimum size of a vertex cover of . The second
lower bound shows that the upper bound is
asymptotically tight. As to the properties of the "right-hand side" graph ,
it is known that HOM can be solved in time and
where is the maximum degree of
and is the treewidth of . This gives
single-exponential algorithms for graphs of bounded maximum degree or bounded
treewidth. Since the chromatic number does not exceed
and , it is natural to ask whether similar
upper bounds with respect to can be obtained. We provide a negative
answer to this question by establishing a lower bound for any
function . We also observe that similar lower bounds can be obtained for
locally injective homomorphisms.Comment: 19 page
Functionally distinct and selectively phosphorylated GPCR subpopulations co-exist in a single cell.
G protein-coupled receptors (GPCRs) transduce pleiotropic intracellular signals in a broad range of physiological responses and disease states. Activated GPCRs can undergo agonist-induced phosphorylation by G protein receptor kinases (GRKs) and second messenger-dependent protein kinases such as protein kinase A (PKA). Here, we characterize spatially segregated subpopulations of β2-adrenergic receptor (β2AR) undergoing selective phosphorylation by GRKs or PKA in a single cell. GRKs primarily label monomeric β2ARs that undergo endocytosis, whereas PKA modifies dimeric β2ARs that remain at the cell surface. In hippocampal neurons, PKA-phosphorylated β2ARs are enriched in dendrites, whereas GRK-phosphorylated β2ARs accumulate in soma, being excluded from dendrites in a neuron maturation-dependent manner. Moreover, we show that PKA-phosphorylated β2ARs are necessary to augment the activity of L-type calcium channel. Collectively, these findings provide evidence that functionally distinct subpopulations of this prototypical GPCR exist in a single cell
Anharmonicity of a Gatemon Qubit with a Few-Mode Josephson Junction
Coherent operation of gate-voltage-controlled hybrid transmon qubits
(gatemons) based on semiconductor nanowires was recently demonstrated. Here we
experimentally investigate the anharmonicity in epitaxial InAs-Al Josephson
junctions, a key parameter for their use as a qubit. Anharmonicity is found to
be reduced by roughly a factor of two compared to conventional metallic
junctions, and dependent on gate voltage. Experimental results are consistent
with a theoretical model, indicating that Josephson coupling is mediated by a
small number of highly transmitting modes in the semiconductor junction
Switchable Fluorescent and Solvatochromic Molecular Probes Based on 4-Amino-N-methylphthalimide and a Photochromic Diarylethene
New fluorescent photochromic compounds (1-H and 1-Boc)have been synthesized and characterized in different solvents.The fluorescence emission can be switched âonâ andâoffâ with visible light and UV, respectively, by means of thephotochromic reaction. The emission wavelength and efficiencystrongly depend on the polarity of the solvent. Thecompounds show a positive solvatochromic effect in theemission maxima, and their fluorescence quantum yield decreasesas the solventâs polarity increases (from cyclohexaneto dioxane). In solvents more polar than dioxane the emissionis too weak and therefore undetectable, and thus 1-H and 1-Boc behave as ânormalâ photochromic compounds. The photochromic reaction is also sensitive to the environment. A decreaseof more than an order of magnitude was found for thequantum yield of the colouring reaction (ÎŚOFCF) for 1-H inethanol compared with cyclohexane, and an about threefolddecrease in ÎŚOFCF was observed for the compound 1-Bocin polar solvents (compared with apolar solvents). For bothcompounds the ring-opening reaction was found not to dependenton the solvent. The novel fluorescent molecularswitches 1-H and 1-Boc are able to probe the polarity of theirmicroenvironment.Fil: Yan, Sergey F.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Max Planck Institute for Biophysical Chemistry; Alemania. Consejo Nacional de Investigaciones CientĂficas y TĂŠcnicas. Oficina de CoordinaciĂłn Administrativa Ciudad Universitaria. Instituto de QuĂmica, FĂsica de los Materiales, Medioambiente y EnergĂa. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de QuĂmica, FĂsica de los Materiales, Medioambiente y EnergĂa; ArgentinaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemani
Subcortical cytoskeleton periodicity throughout the nervous system
Superresolution fluorescence microscopy recently revealed a ~190ânm periodic cytoskeleton lattice consisting of actin, spectrin, and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is a structural feature of all neurons and how it is modified when axons are ensheathed by myelin forming glial cells is not known. Here, STED nanoscopy is used to demonstrate that this structure is a commonplace of virtually all neuron types in vitro. To check how the subcortical meshwork is modified during myelination, we studied sciatic nerve fibers from adult mice. Periodicity of both actin and spectrin was uncovered at the internodes, indicating no substantial differences between unmyelinated and myelinated axons. Remarkably, the actin/spectrin pattern was also detected in glial cells such as cultured oligodendrocyte precursor cells. Altogether our work shows that the periodic subcortical cytoskeletal meshwork is a fundamental characteristic of cells in the nervous system and is not a distinctive feature of neurons, as previously thought
- âŚ