The erythrocytic schizogony of two synchronized strains of Plasmodium berghei, NK65 and ANKA, in normocytes and reticulocytes

By a modified Percoll-glucose centrifugation technique the rings and young trophozoites of two strains of Plasmodium berghei, NK65 and ANKA, were separated from the other erythrocytic stages and inoculated into mice. The subsequent infection was followed for ANKA in normal mice and for NK65 in normal mice and in mice with high-grade reticulocytosis induced by injections of phenylhydrazine. The duration of the erythrocytic schizogony of the NK65 strain was shown to be independent of the age of the host cell, and the hour of inoculation did not influence the cycle of the ANKA strain

Pathways between childhood trauma, intimate partner violence, and harsh parenting : findings from the UN multi-country study on men and violence in Asia and the Pacific

2016-2017 > Academic research: refereed > Publication in refereed journal201804_a bcmaVersion of RecordPublishe

Sertoli cells maintain leydig cell number and peritubular myoid cell activity in the adult mouse testis

The Sertoli cells are critical regulators of testis differentiation and development. In the adult, however, their known function is restricted largely to maintenance of spermatogenesis. To determine whether the Sertoli cells regulate other aspects of adult testis biology we have used a novel transgenic mouse model in which Amh-Cre induces expression of the receptor for Diphtheria toxin (iDTR) specifically within Sertoli cells. This causes controlled, cell-specific and acute ablation of the Sertoli cell population in the adult animal following Diphtheria toxin injection. Results show that Sertoli cell ablation leads to rapid loss of all germ cell populations. In addition, adult Leydig cell numbers decline by 75% with the remaining cells concentrated around the rete and in the sub-capsular region. In the absence of Sertoli cells, peritubular myoid cell activity is reduced but the cells retain an ability to exclude immune cells from the seminiferous tubules. These data demonstrate that, in addition to support of spermatogenesis, Sertoli cells are required in the adult testis both for retention of the normal adult Leydig cell population and for support of normal peritubular myoid cell function. This has implications for our understanding of male reproductive disorders and wider androgen-related conditions affecting male health

Risk of HIV transmission through condomless sex in serodifferent gay couples with the HIV-positive partner taking suppressive antiretroviral therapy (PARTNER): final results of a multicentre, prospective, observational study.

Peer reviewe